| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-Hom-Mem1 |
| 质粒类型: | 慢病毒载体；双顺反子载体；信号通路载体；亚细胞定位载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 8411 bp |
| 5' 测序引物及序列: | CMV Forward： CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | DmrB |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | 慢病毒载体pLVX-Hom-Mem1表达DmrB融合蛋白；利用信号受体的二聚化作用研究细胞信号通路；DmrB蛋白加了膜定位信号。 |
| 产品目录号: | 635073 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
注：
When cloning your gene of interest into the pLVX-Hom-Mem1 vector, make certain that the N-myr-signal is located on the N-terminus of the DmrB fusion protein. The N‐myr‐signal is NOT functional if localized in the middle or at the C‐terminus of a protein of interest. Your gene must either be cloned into the 3’MCS, or if cloned into the 5’ MCS, which is located upstream of the N‐myr signal, you must include a separate N‐myr signal sequence on the forward primer used to amplify your gene of interest.
载体序列
LOCUS pLVX-Hom-Mem1 8841 bp ds-DNA circular SYN 30-JUL-2013
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
KEYWORDS pLVX-Hom-Mem1 Vector
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8841)
AUTHORS Clontech Laboratories Inc.
FEATURES Location/Qualifiers
source 1..8841
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label="5' LTR"
misc_feature 681..806
/label="Packaging signal"
misc_feature 1303..1536
/label="RRE"
misc_feature 2028..2144
/label="cPPT/CTS"
enhancer 2201..2504
/label="CMV enhancer"
promoter 2505..2708
/label="CMV promoter"
misc_feature 2803..2820
/label="5' MCS"
misc_feature 2821..2862
/label="N-myr signal"
CDS 2869..3189
/label="DmrB"
CDS 3196..3516
/label="DmrB"
misc_feature 3518..3531
/label="3' MCS"
misc_feature 3544..4117
/label="IRES"
CDS 4150..4749
/label="PuroR"
misc_feature 4763..5351
/label="WPRE"
LTR 5559..6192
/label="3' LTR"
rep_origin complement(6723..7308)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7479..8339)
/label="AmpR"
promoter complement(8340..8444)
/label="AmpR promoter"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt
2821 atggggagta gcaagagcaa gcctaaggac cccagccagc gctctagagg cgtccaagtc
2881 gaaaccatta gtcccggcga tggcagaaca tttcctaaaa ggggacaaac atgtgtcgtc
2941 cattatacag gcatgttgga ggacggcaaa aaggtggaca gtagtagaga tcgcaataaa
3001 cctttcaaat tcatgttggg aaaacaagaa gtcattaggg gatgggagga gggcgtggct
3061 caaatgtccg tcggccaacg cgctaagctc accatcagcc ccgactacgc atacggcgct
3121 accggacatc ccggaattat tccccctcac gctaccttgg tgtttgacgt cgaactgttg
3181 aagctcgaga ctagaggagt gcaggtggag actatctccc caggagacgg gcgcaccttc
3241 cccaagcgcg gccagacctg cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa
3301 gttgattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa gcaggaggtg
3361 atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc caaactgact
3421 atatctccag attatgccta tggtgccact gggcacccag gcatcatccc accacatgcc
3481 actctcgtct tcgatgtgga gcttctaaaa ctggaaagcg gccgcggatc ctacgtatga
3541 ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
3601 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
3661 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
3721 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
3781 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
3841 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
3901 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
3961 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
4021 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
4081 cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc cacaaggaga
4141 cgaccttcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
4201 cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
4261 gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
4321 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
4381 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
4441 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
4501 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
4561 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
4621 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
4681 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
4741 ggtgcctaga cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac
4801 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
4861 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
4921 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
4981 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
5041 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
5101 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
5161 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
5221 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
5281 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
5341 cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca
5401 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
5461 gaggtgggtt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
5521 tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca
5581 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
5641 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
5701 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
5761 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
5821 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
5881 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
5941 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
6001 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
6061 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
6121 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
6181 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
6241 aatgaatatc agagagtgag aggccttgac attgctagcg ttttaccgtc gacctctagc
6301 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
6361 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
6421 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
6481 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
6541 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
6601 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
6661 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
6721 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
6781 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
6841 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
6901 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
6961 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
7021 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
7081 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
7141 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
7201 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
7261 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
7321 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
7381 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
7441 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
7501 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
7561 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
7621 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
7681 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
7741 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
7801 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
7861 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
7921 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
7981 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
8041 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
8101 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
8161 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
8221 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
8281 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
8341 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
8401 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
8461 gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta
8521 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
8581 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
8641 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
8701 agtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
8761 gtatttgtta aatatgtact acaaacttag tagtttttaa agaaattgta tttgttaaat
8821 atgtactaca aacttagtag t
//
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