| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pDONR221-P5P2 |
| 质粒类型: | Mltisite-Gateway载体；DONR载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | 无 |
| 克隆方法: | Gateway；BP反应 |
| 载体大小: | 4774bp |
| 5' 测序引物及序列: | M13/pUC fwd 5'CCCAGTCACGACGTTGTAAAACG 3' |
| 3' 测序引物及序列: | M13/pUC rev 5' AGCGGATAACAATTTCACACAGG 3' |
| 载体标签: | 无 |
| 载体抗性: | 卡那霉素，氯霉素（仅空载体） |
| 真核筛选标记: | 无 |
| 克隆菌株: | 阳性筛选用DB3.1 ，阴性筛选用OmniMAX2-T1 |
| 备注: |
pDONR221-P5P2载体是MultiSite Gateway载体， 目标载体如 pAd/PL-DEST、pcDNA6/V5-PL-DEST载体，是研究启动子的理想工具。 |
| 产品目录号: | 12537-102 |
| 病毒/非病毒: | 非病毒 |
买家导航
pDONR221-P5P2载体质粒基本信息
pDONR221-P5P2质粒图谱载体图谱和pDONR221-P5P2载体序列质粒序列多克隆位点信息
pDONR221-P5P2质粒载体简介
pDONR221-P5P2质粒序列载体序列
LOCUS Exported 4774 bp ds-DNA circular SYN 10-AUG-2016
DEFINITION synthetic circular DNA
KEYWORDS pDONR221-P5P2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4774)
AUTHORS Biofeng Lab.
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..4774
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 268..295
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 387..473
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 537..553
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 570..801
/gene="mutant version of attP"
/bound_moiety="BP Clonase(TM)"
/note="attP5"
/note="recombination site for the Gateway(R) BP reaction"
CDS complement(1197..1502)
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/note="ccdB"
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
CDS complement(1852..2505)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG"
promoter complement(2506..2608)
/note="cat promoter"
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
protein_bind complement(2753..2984)
/gene="mutant version of attP"
/bound_moiety="BP Clonase(TM)"
/note="attP2"
/note="recombination site for the Gateway(R) BP reaction
(pDONR(TM)201 version)"
promoter complement(3016..3034)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3039..3055)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 3168..3977
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 4124..4712
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtatacaaa
661 agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
721 aaaacagact acataatact gtaaaacaca acatatccag tcactatgaa tcaactactt
781 agatggtatt agtgacctgt agtcgaccga cagccttcca aatgttcttc gggtgatgct
841 gccaacttag tcgaccgaca gccttccaaa tgttcttctc aaacggaatc gtcgtatcca
901 gcctactcgc tattgtcctc aatgccgtat taaatcataa aaagaaataa gaaaaagagg
961 tgcgagcctc ttttttgtgt gacaaaataa aaacatctac ctattcatat acgctagtgt
1021 catagtcctg aaaatcatct gcatcaagaa caatttcaca actcttatac ttttctctta
1081 caagtcgttc ggcttcatct ggattttcag cctctatact tactaaacgt gataaagttt
1141 ctgtaatttc tactgtatcg acctgcagac tggctgtgta taagggagcc tgacatttat
1201 attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca
1261 gccacttctt ccccgataac ggagaccggc acactggcca tatcggtggt catcatgcgc
1321 cagctttcat ccccgatatg caccaccggg taaagttcac gggagacttt atctgacagc
1381 agacgtgcac tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc
1441 tgtacatcca caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc
1501 atttcaccag cccctgttct cgtcagcaaa agagccgttc atttcaataa accgggcgac
1561 ctcagccatc ccttcctgat tttccgcttt ccagcgttcg gcacgcagac gacgggcttc
1621 attctgcatg gttgtgctta ccagaccgga gatattgaca tcatatatgc cttgagcaac
1681 tgatagctgt cgctgtcaac tgtcactgta atacgctgct tcatagcata cctctttttg
1741 acatacttcg ggtatacata tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat
1801 acgcatactg ttatctggct tttagtaagc cggatccacg cggcgtttac gccccccctg
1861 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
1921 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
1981 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
2041 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
2101 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
2161 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
2221 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
2281 catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
2341 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
2401 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
2461 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
2521 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
2581 aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg
2641 cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac
2701 aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc aacttagtcg actacaggtc
2761 actaatacca tctaagtagt tgattcatag tgactggata tgttgtgttt tacagtatta
2821 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
2881 ttctcgttca gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
2941 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgcagg
3001 gcggccgcga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
3061 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
3121 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
3181 aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
3241 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc
3301 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
3361 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
3421 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat
3481 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
3541 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
3601 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
3661 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc
3721 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
3781 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
3841 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
3901 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
3961 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc
4021 tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt
4081 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
4141 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
4201 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
4261 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
4321 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
4381 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
4441 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
4501 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
4561 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
4621 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
4681 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
4741 tcctggcctt ttgctggcct tttgctcaca tgtt
//
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