pLKO.1-puro

下面是pLKO.1-puro载体序列，AgeI和EcorI之间含有一个18 bp的shRNA插入片段情况下的载体序列。

LOCUS       pLKO.1 puro        7050 bp    DNA     circular     15-JUL-2022
DEFINITION  pLKO.1 puro
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|-14481672|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLKO.1 puro|
COMMENT     VNTAUTHORNAME|Biofeng.com|
COMMENT     VNTAUTHOREML|order@biofeng.com|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="pLKO.1 puro"
                     /mol_type="other DNA"
                     /vntifkey="98"
     CDS             289..1128
                     /vntifkey="4"
                     /label=ORF\frame\1
     CDS             complement(334..1179)
                     /vntifkey="4"
                     /label=ORF\frame\1
     gene            529..1128
                     /gene="puro (variant)"
                     /vntifkey="60"
     misc_feature    1239..1260
                     /vntifkey="21"
                     /label=U3PPT
     misc_feature    1239..1254
                     /vntifkey="21"
                     /label=cPPT
     misc_feature    1256..1308
                     /vntifkey="21"
                     /label=delta_U3
     misc_feature    1309..1489
                     /vntifkey="21"
                     /label=HIV-1_5_LTR
     misc_feature    1309..1489
                     /vntifkey="21"
                     /label=truncHIV-1_3_LTR
     terminator      1564..1683
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    1652..1671
                     /vntifkey="21"
                     /label=EBV_rev_primer
     rep_origin      1701..1799
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    1784..1803
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     promoter        complement(1878..1896)
                     /vntifkey="30"
                     /label=T7_promoter
     promoter        complement(1906..1922)
                     /vntifkey="30"
                     /label=M13_forward20_primer
     misc_feature    complement(1915..1937)
                     /vntifkey="21"
                     /label=M13_pUC_fwd_primer
     misc_feature    1915..2063
                     /vntifkey="21"
                     /label=lacZ_a
     rep_origin      2081..2387
                     /vntifkey="33"
                     /label=f1_origin
     promoter        2580..2608
                     /vntifkey="30"
                     /label=AmpR_promoter
     gene            2650..3510
                     /gene="Ampicillin"
                     /vntifkey="60"
     CDS             2650..3510
                     /vntifkey="4"
                     /label=ORF\frame\1
     rep_origin      3665..4284
                     /vntifkey="33"
                     /label=pBR322_origin
     promoter        4593..4622
                     /vntifkey="30"
                     /label=lac_promoter
     misc_feature    4636..4658
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        4657..4675
                     /vntifkey="30"
                     /label=M13_reverse_primer
     promoter        4692..4711
                     /vntifkey="30"
                     /label=T3_promoter
     misc_feature    4965..5145
                     /vntifkey="21"
                     /label=HIV-1_5_LTR
     misc_feature    4965..5145
                     /vntifkey="21"
                     /label=truncHIV-1_3_LTR
     misc_feature    5256..5300
                     /vntifkey="21"
                     /label=HIV-1_psi_pack
     CDS             5688..6545
                     /vntifkey="4"
                     /label=ORF\frame\3
     misc_feature    5810..6043
                     /vntifkey="21"
                     /label=RRE
     promoter        6579..6811
                     /vntifkey="30"
                     /label=hU6_promoter
     misc_feature    6741..6760
                     /vntifkey="21"
                     /label=LKO1_5_primer
     misc_feature    6885..6900
                     /vntifkey="21"
                     /label=cPPT
     CDS             complement(6966..530)
                     /vntifkey="4"
                     /label=ORF\frame\2
BASE COUNT     1803 a      1748 c      1862 g      1637 t 
ORIGIN
        1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 
       61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 
      121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 
      181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 
      241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 
      301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 
      361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 
      421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 
      481 gaccgaatca ccgacctctc tccccagggg gatccaccgg agcttaccat gaccgagtac 
      541 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 
      601 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 
      661 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 
      721 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 
      781 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 
      841 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 
      901 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 
      961 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 
     1021 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 
     1081 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac 
     1141 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcggtacc tttaagacca 
     1201 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 
     1261 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg 
     1321 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 
     1381 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 
     1441 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca 
     1501 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg 
     1561 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 
     1621 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 
     1681 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc 
     1741 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 
     1801 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 
     1861 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt 
     1921 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 
     1981 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 
     2041 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 
     2101 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 
     2161 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 
     2221 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 
     2281 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 
     2341 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 
     2401 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 
     2461 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 
     2521 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 
     2581 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 
     2641 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 
     2701 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 
     2761 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 
     2821 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 
     2881 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 
     2941 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 
     3001 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 
     3061 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 
     3121 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 
     3181 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 
     3241 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 
     3301 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 
     3361 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 
     3421 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 
     3481 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 
     3541 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 
     3601 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 
     3661 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 
     3721 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 
     3781 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 
     3841 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 
     3901 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 
     3961 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 
     4021 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 
     4081 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 
     4141 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 
     4201 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 
     4261 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 
     4321 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 
     4381 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 
     4441 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 
     4501 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 
     4561 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 
     4621 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 
     4681 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt 
     4741 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct 
     4801 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg 
     4861 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc 
     4921 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag 
     4981 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 
     5041 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 
     5101 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag 
     5161 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga 
     5221 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 
     5281 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 
     5341 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 
     5401 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 
     5461 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 
     5521 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 
     5581 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 
     5641 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 
     5701 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 
     5761 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 
     5821 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg 
     5881 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 
     5941 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 
     6001 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 
     6061 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 
     6121 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca 
     6181 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag 
     6241 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc 
     6301 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt 
     6361 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga 
     6421 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag 
     6481 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag 
     6541 actagcctcg agcggccgcc cccttcaccg agggcctatt tcccatgatt ccttcatatt 
     6601 tgcatatacg atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa 
     6661 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt 
     6721 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt 
     6781 tcttggcttt atatatcttg tggaaaggac gaaacaccgg tccgcaggta tgcacgcgtg 
     6841 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga 
     6901 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta 
     6961 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca 
     7021 gagatccact ttggccgcgg ctcgaggggg 
//