| 出品公司: | Addgene |
|---|---|
| 载体名称: | pLKO.3G |
| 质粒类型: | 慢病毒系统载体；RNAi |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | U6 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8174bp |
| 5' 测序引物及序列: | GACTATCATATGCTTACCGT |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillian |
| 筛选标记: | eGFP |
| 备注: | 克隆菌株Stbl3 |
| 产品目录号: | 14748 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | 慢病毒 |
买家导航
pLKO.3G载体质粒基本信息
pLKO.3G质粒图谱载体图谱和pLKO.3G载体序列质粒序列多克隆位点信息
pLKO.3G质粒载体简介
pLKO.3G质粒序列载体序列
LOCUS pLKO.3G 8174 bp DNA SYN
DEFINITION pLKO.3G
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..8174
/organism="pLKO.3G"
/mol_type="other DNA"
misc_feature 168..183
/label="cPPT"
CDS 693..1652
/label="ORF frame 3"
gene 936..1649
/label="EGFP"
/gene="EGFP"
misc_feature complement(978..999)
/label="EGFP_N_primer"
misc_feature 1116..1145
/label="Y66 (EGFP)"
misc_feature 1586..1607
/label="EGFP_C_primer"
misc_feature 1673..2260
/label="WPRE"
CDS 1761..2312
/label="ORF frame 3"
CDS 1774..2544
/label="ORF frame 1"
misc_feature complement(2263..2279)
/label="pBluescriptKS_primer"
misc_feature 2448..2469
/label="U3PPT"
misc_feature 2448..2463
/label="cPPT"
misc_feature 2465..2517
/label="delta_U3"
misc_feature 2518..2698
/label="HIV-1_5_LTR"
misc_feature 2518..2698
/label="truncHIV-1_3_LTR"
terminator 2773..2892
/label="SV40_PA_terminator"
misc_feature 2861..2880
/label="EBV_rev_primer"
rep_origin 2910..3008
/label="SV40_origin"
misc_feature 2993..3012
/label="SV40pro_F_primer"
promoter complement(3087..3105)
/label="T7_promoter"
promoter complement(3115..3131)
/label="M13_forward20_primer"
misc_feature complement(3124..3146)
/label="M13_pUC_fwd_primer"
misc_feature 3124..3272
/label="lacZ_a"
rep_origin 3290..3596
/label="f1_origin"
promoter 3789..3817
/label="AmpR_promoter"
gene 3859..4719
/label="Ampicillin"
/gene="Ampicillin"
CDS 3859..4719
/label="ORF frame 1"
rep_origin 4874..5493
/label="pBR322_origin"
promoter 5802..5831
/label="lac_promoter"
misc_feature 5845..5867
/label="M13_pUC_rev_primer"
promoter 5866..5884
/label="M13_reverse_primer"
promoter 5901..5920
/label="T3_promoter"
misc_feature 6174..6354
/label="HIV-1_5_LTR"
misc_feature 6174..6354
/label="truncHIV-1_3_LTR"
misc_feature 6465..6509
/label="HIV-1_psi_pack"
CDS 6897..7754
/label="ORF frame 3"
misc_feature 7019..7252
/label="RRE"
promoter 7983..65
/label="hU6_promoter"
misc_feature 8169..14
/label="LKO1_5_primer"
ORIGIN
1 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
61 ggacgaaaca ccggtccgca ggtatgcttg cgtgaattcc acctcgagct acttaattaa
121 accagttttc gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg
181 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
241 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
301 gcagagatcc actttggccg cggctcgagg gggttggggt tgcgcctttt ccaaggcagc
361 cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc
421 gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct
481 acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg
541 cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca
601 gacggacagc gccagggagc aatggcagcg cgccgaccgc ggaagccgca cgtctcacta
661 gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg
721 tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc
781 gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat
841 tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc
901 tctctcccca gggggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca
961 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
1021 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
1081 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
1141 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
1201 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
1261 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
1321 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
1381 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
1441 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
1501 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
1561 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
1621 tcactctcgg catggacgag ctgtacaagt aagatatcaa gcttatcgat aatcaacctc
1681 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
1741 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
1801 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
1861 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
1921 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
1981 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
2041 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
2101 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
2161 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
2221 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
2281 aacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
2341 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
2401 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
2461 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
2521 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
2581 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
2641 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
2701 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
2761 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
2821 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
2881 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
2941 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
3001 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
3061 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
3121 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
3181 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
3241 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
3301 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
3361 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
3421 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
3481 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
3541 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
3601 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
3661 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
3721 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
3781 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
3841 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
3901 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
3961 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
4021 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
4081 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
4141 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
4201 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
4261 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
4321 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
4381 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
4441 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
4501 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
4561 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
4621 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
4681 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
4741 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
4801 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
4861 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
4921 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
4981 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
5041 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
5101 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
5161 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
5221 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
5281 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
5341 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
5401 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
5461 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
5521 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
5581 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
5641 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
5701 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
5761 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
5821 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
5881 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
5941 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
6001 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
6061 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
6121 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
6181 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
6241 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
6301 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
6361 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
6421 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
6481 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
6541 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
6601 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
6661 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
6721 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
6781 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
6841 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
6901 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
6961 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
7021 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
7081 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
7141 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
7201 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
7261 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
7321 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
7381 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
7441 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
7501 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
7561 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
7621 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
7681 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
7741 gatcacgaga ctagcctcga ccacgacgca atggagaaag acggagagcg ccaacggcgt
7801 ccatctcgaa ggagtcgcca gcgataaccg gagtagttga aatggtaata agacgaccaa
7861 tctgaccagc aaggaagcca agatgggaaa ggtcatgcgg catacgctcg gcgccagttt
7921 gaatattaga cataatttat cctcaagtaa gggggtacac gcgtcagtcg agtcctgtcg
7981 acaaggtcgg gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat
8041 acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag atattagtac
8101 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
8161 ttaaaatgga ctat
//
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