| 质粒名称: | pGP-CMV-jGCaMP8m |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 162372 |
| 存储实验室: | GENIE Project |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | ThermoFisher Invitrogen |
| 空载体大小: | -- |
| 完整质粒大小: | 5224 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达 |
| 筛选标记: | 新霉素（G418筛选） |
| 载体抗性: | 卡那霉素，50μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | jGCaMP8m |
| 物种来源: | R. norvegicus (rat), G. gallus (chicken); A. victoria (jellyfish) |
| 插入大小: | 1269 bp |
| 基因突变: | A25G F286Y |
| GenBank编号 | -- |
| 启动子: | CMV |
| 标签/融合蛋白: | 6xHis（插入件上的N端子） |
| 克隆方法: | Gibson Cloning |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pGP-CMV-jGCaMP8m was a gift from GENIE Project (Addgene plasmid # 162372 ; http://n2t.net/addgene:162372 ; RRID:Addgene_162372) |
| 参考文献部分: | jGCaMP8 Fast Genetically Encoded Calcium Indicators. Zhang , Yan; Rózsa, Márton; Bushey, Daniel; Zheng, Jihong; Reep, Daniel; Broussard, Gerard Joey; Tsang, Arthur; Tsegaye, Getahun; Patel, Ronak; Narayan, Sujatha; Li, Jing Xuan; Zhang, Rongwei; Ahrens, Misha B.; Turner, Glenn C.; Wang, Samuel S.-H.; Svoboda, Karel; Korff, Wyatt; Schreiter, Eric R.; Hasseman, Jeremy P.; Kolb, Ilya; Looger, Loren L. Janelia Research Campus. Online resource. 2020 10.25378/janelia.13148243.v1 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGP-CMV-jGCaMP8m 5224 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Mammalian expression of ultrafast protein calcium sensor.
ACCESSION .
VERSION .
KEYWORDS pGP-CMV-jGCaMP8m
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5224)
AUTHORS Zhang , Yan; Rozsa, Marton; Bushey, Daniel; Zheng, Jihong; Reep,
Daniel; Broussard, Gerard Joey; Tsang, Arthur; Tsegaye, Getahun;
Patel, Ronak; Narayan, Sujatha; Li, Jing Xuan; Zhang, Rongwei;
Ahrens, Misha B.; Turner, Glenn C.; Wang, Samuel S.-H.; Svoboda,
Karel; Korff, Wyatt; Schreiter, Eric R.; Hasseman, Jeremy P.; Kolb,
Ilya; Looger, Loren L
TITLE jGCaMP8 Fast Genetically Encoded Calcium Indicators
JOURNAL Janelia Research Campus. Online resource. 2020
REFERENCE 2 (bases 1 to 5224)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5224
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 86..389
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 390..593
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 544..564
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 650..667
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind 949..970
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(1076..1097)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1337..1356)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
polyA_signal 2037..2158
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2074..2093)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2128..2147
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2165..2620)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2302..2323)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2514..2533
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2647..2751
/gene="bla"
/label=AmpR promoter
promoter 2753..3110
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2776..2796)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2961..3096
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3023..3042
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3145..3939
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3199..3218)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3809..3828
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4127..4146)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4171..4218
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4547..5135
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5036..5055
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ggataaccgt attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag
61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
601 tgaaccgtca gatccgctag cgctaccgga ctcagatctc gccaccatgc atcatcacca
661 tcatcacacg cgtcgcaaga agaccttcaa ggaggtggcc accgccgtga agatcatcgg
721 ccgcctgatg ggcctgaaga tcaacgtcta tatcaaggcc gacaagcaga agaacggcat
781 caaggcgaac ttccacatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca
841 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
901 gagcgtggag tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct
961 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac
1021 cggagggagc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
1081 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga
1141 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
1201 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
1261 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg
1321 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
1381 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
1441 cctggggcac aagctggagt acaacctgcc ggaccaactg actgaagagc agatcgcaga
1501 atacaaagag gctttctccc tatttgacaa ggacggggat gggacaataa caaccaagga
1561 gctggggacg gtgatgcggt ctctggggca gaaccccaca gaagcagagc tgcaggacat
1621 gatcaatgaa gtagatgccg acggtgacgg cacaatcgac ttccctgagt tcctgacaat
1681 gatggcaaga aaaatgaaat acagggacac ggaagaagaa attagagaag cgttcggtgt
1741 gtttgataag gatggcaatg gctacatcag tgcagcagag cttcgccacg tgatgacaaa
1801 ccttggagag aagttaacag atgaagaggt tgatgaaatg atcagggaag cagacatcga
1861 tggggatggt caggtaaact acgaagagtt tgtacaaatg atgacagcga agtaagcggc
1921 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
1981 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
2041 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
2101 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag
2161 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
2221 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
2281 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
2341 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
2401 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
2461 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
2521 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
2581 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
2641 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
2701 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
2761 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
2821 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
2881 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
2941 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
3001 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
3061 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
3121 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
3181 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
3241 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
3301 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
3361 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
3421 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
3481 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
3541 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
3601 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
3661 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
3721 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
3781 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
3841 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
3901 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
3961 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
4021 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
4081 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
4141 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
4201 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
4261 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
4321 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
4381 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
4441 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
4501 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
4561 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
4621 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
4681 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
4741 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
4801 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
4861 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
4921 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
4981 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
5041 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
5101 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
5161 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
5221 ctgt
//
