质粒名称: | pPAmCherry1-C1 |
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出品公司: | Addgene |
目录编号: | 31929 |
存储实验室: | Vladislav Verkhusha |
载体骨架基本信息 |
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载体名称: | pEGFP-C1 |
载体出品公司: | Clontech |
载体抗性: | 卡那霉素 |
空载体大小: | 4008 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 哺乳细胞表达 |
筛选标记: | 新霉素(G418) |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | PAmCherry1,photoactivatable dark-to-red mCherry red fluorescent protein |
物种来源: | -- |
插入大小: | 708 bp |
基因突变: | E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/I203R compared to mCherry (but numbering is relative to EGFP). |
GenBank编号 | -- |
启动子 | CMV |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | NheI(销毁时未知) |
3' 克隆位点: | BsrGI(销毁时未知) |
5' 测序引物 | cggtaggcgtgtacggtgggag |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pPAmCherry1-C1 was a gift from Vladislav Verkhusha (Addgene plasmid # 31929 ; http://n2t.net/addgene:31929 ; RRID:Addgene_31929) |
参考文献部分: | Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Subach FV, Patterson GH, Manley S, Gillette JM, Lippincott-Schwartz J, Verkhusha VV. Nat Methods. 2009 Feb;6(2):153-9. Epub 2009 Jan 25. 10.1038/nmeth.1298 PubMed 19169259 |
买家导航
pPAmCherry1C1载体基本信息
pPAmCherry1C1载体图谱质粒图谱和多克隆位点信息
pPAmCherry1C1载体简介
pPAmCherry1C1载体序列
LOCUS pPAmCherry1-C1 4716 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pPAmCherry1-C1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4716) AUTHORS Subach FV, Patterson GH, Manley S, Gillette JM, Lippincott-Schwartz J, Verkhusha VV TITLE Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. JOURNAL Nat Methods. 2009 Feb;6(2):153-9. Epub 2009 Jan 25. PUBMED 19169259 REFERENCE 2 (bases 1 to 4716) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.biofeng.com/ FEATURES Location/Qualifiers source 1..4716 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 519..539 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 601..610 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 607..1314 /codon_start=1 /product="photoactivatable monomeric derivative of DsRed fluorescent protein (Subach et al., 2009)" /label=PAmCherry1 /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(752..770) /label=mCherry-R /note="mCherry, reverse primer" misc_feature 1315..1380 /label=MCS /note="multiple cloning site" polyA_signal 1504..1625 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1541..1560) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1595..1614 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1632..2087) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1769..1790) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1981..2000 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2114..2218 /gene="bla" /label=AmpR promoter promoter 2220..2577 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2243..2263) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2428..2563 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2490..2509 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2612..3406 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2666..2685) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3276..3295 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3594..3613) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3638..3685 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4014..4602 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4503..4522 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcggtc 601 gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcattaagga gttcatgcgc 661 ttcaaggtgc acatggaggg gtccgtgaac ggccacgtgt tcgagatcga gggcgagggc 721 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc 781 ctgcccttca cctgggacat cctgagccct cagttcatgt acggctccaa tgcctacgtg 841 aagcaccccg ccgacatccc cgactacttt aagctgtcct tccccgaggg cttcaagtgg 901 gagcgcgtga tgaaattcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg 961 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc 1021 cccgtgatgc agaagaagac catgggctgg gaggccctct ccgagcggat gtaccccgag 1081 gacggcgccc tgaagggcga ggtcaagccc agagtgaagc tgaaggacgg cggccactac 1141 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac 1201 aacgtcaacc gcaagctgga catcaccagc cacaacgagg actacaccat cgtggagcag 1261 tacgagagag ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtccgga 1321 ctcagatctc gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc 1381 accggatcta gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct 1441 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt 1501 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 1561 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 1621 tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta 1681 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 1741 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 1801 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 1861 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 1921 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 1981 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 2041 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt 2101 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 2161 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc 2221 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 2281 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 2341 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 2401 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 2461 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 2521 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat 2581 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg 2641 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 2701 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 2761 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct 2821 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 2881 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 2941 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 3001 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 3061 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 3121 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 3181 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 3241 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 3301 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 3361 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg 3421 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc 3481 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc 3541 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca 3601 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa 3661 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct 3721 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca 3781 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc 3841 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa 3901 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 3961 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 4021 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 4081 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 4141 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 4201 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 4261 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 4321 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 4381 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 4441 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 4501 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 4561 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 4621 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 4681 cctgattctg tggataaccg tattaccgcc atgcat //
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