| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pCMV-SPORT6.1 |
| 质粒类型: | 哺乳动物表达载体；cDNA克隆载体；I.M.A.G.E.载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点；Gateway载体 |
| 启动子: | CMV |
| 载体大小: | 4177 bp |
| 5' 测序引物及序列: | CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’ |
| 3' 测序引物及序列: | SV40pA rev 5'GAAATTTGTGATGCTATTGC' |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 哺乳动物细胞 |
| 备注: |
pCMV-SPORT6.1有多个限制性酶切位点，又可用于Gateway克隆，用于cDNA文库的构建和表达。 |
| 产品目录号: | -- |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pCMV-SPORT6.1载体质粒基本信息
pCMV-SPORT6.1质粒图谱载体图谱和pCMV-SPORT6.1载体序列质粒序列多克隆位点信息
pCMV-SPORT6.1质粒载体简介
pCMV-SPORT6.1质粒序列载体序列
LOCUS Exported 4177 bp ds-DNA circular SYN 12-AUG-2016
DEFINITION Gateway(R) library vector for cloning and transient mammalian cell
expression of cDNAs. See pCMV SPORT6 for an older version of this
vector.
KEYWORDS pCMV SPORT6.1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4177)
AUTHORS Invitrogen (Life Technologies)
COMMENT The cDNA is inserted between bases 781-782, destroying an EcoRV site
present in the original vector.
FEATURES Location/Qualifiers
source 1..4177
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
polyA_signal 125..259
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind 664..680
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 691..709
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 711..735
/gene="mutant version of attB"
/bound_moiety="BP Clonase(TM)"
/note="attB2"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 736..806
/note="MCS"
protein_bind complement(807..831)
/gene="mutant version of attB"
/bound_moiety="BP Clonase(TM)"
/note="attB1"
/note="recombination site for the Gateway(R) BP reaction"
promoter complement(832..850)
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(861..877)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(1003..1206)
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer 1207..1510
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
rep_origin complement(1970..2558)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2729..3589)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3590..3694)
/gene="bla"
/note="AmpR promoter"
rep_origin complement(3721..4176)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
61 attacgccag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
121 gatcgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
181 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
241 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
301 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
361 tgatcatgaa cagactgtga ggactgaggg gcctgaaatg agccttggga ctgtgaatct
421 aaaatacaca aacaattaga atcactagct cctgtgtata atattttcat aaatcatact
481 cagtaagcaa aactctcaag cagcaagcat atgcagctag tttaacacat tatacactta
541 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca
601 caccacagaa gtaaggttcc ttcacaaaga tcccaagcta gcagttttcc cagtcacgac
661 gttgtaaaac gacggccagt gcctagctta taatacgact cactataggg accactttgt
721 acaagaaagc tgggtacgcg taagcttggg cccctcgagg gatactctag agcggccgcc
781 catcccggga attccggacc ggtaccagcc tgcttttttg tacaaacttg ttctatagtg
841 tcacctaaat aggcctaatg gtcatagctg tttcctgtgt gaaattgtta tccgctccgc
901 ggcctaggct agagtccgga ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca
961 gcgtggatgg cgtctccagg cgatctgacg gttcactaaa cgagctctgc ttatatagac
1021 ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt
1081 tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag
1141 acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg
1201 catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca
1261 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
1321 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
1381 ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat
1441 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
1501 ttatgtaacg acatgcatct aatgagtgaa agggcctcgt actacgccta tttttatagg
1561 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
1621 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
1681 aataaccctg ataaatgctt caataatatt gaaaaacgcg cgaattgcaa gctctgcatt
1741 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
1801 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
1861 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
1921 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
1981 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
2041 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
2101 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
2161 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
2221 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
2281 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
2341 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
2401 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
2461 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
2521 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
2581 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
2641 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
2701 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
2761 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
2821 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
2881 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
2941 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
3001 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
3061 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
3121 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
3181 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
3241 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
3301 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
3361 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
3421 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
3481 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
3541 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
3601 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
3661 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
3721 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
3781 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
3841 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
3901 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
3961 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
4021 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
4081 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
4141 cacccgccgc gcttaatgcg ccgctacagg gcgcgtc
//
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