质粒名称: | pSinREP5-iAchSnFR |
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出品公司: | Addgene |
目录编号: | 140643 |
存储实验室: | J. Julius Zhu |
载体骨架基本信息 |
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载体名称: | pSinREP5 |
载体出品公司: | -- |
空载体大小: | 9951 bp |
完整质粒大小: | 11826 bp |
载体修饰: | -- |
载体类型: | 哺乳细胞表达 |
筛选标记: | -- |
载体抗性: | 氨苄霉素 |
生长温度: | 37 ℃ |
宿主菌: | NEB Stable |
拷贝数: | -- |
插入基因信息 |
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插入基因名称: | Ach sensor V9 |
物种来源: | -- |
插入大小: | 1844 bp |
基因突变: | -- |
GenBank编号 | -- |
启动子: | SP6 |
标签/融合蛋白: | -- |
克隆方法: | Gibson克隆 |
5’克隆位点: | -- |
3’克隆位点: | -- |
5’测序引物 | AGCATAGTACATTTCATCTG |
3’测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pSinREP5-iAchSnFR was a gift from J. Julius Zhu (Addgene plasmid # 140643 ; http://n2t.net/addgene:140643 ; RRID:Addgene_140643) |
买家导航
pSinREP5iAchSnFR载体基本信息
pSinREP5iAchSnFR载体图谱质粒图谱和多克隆位点信息
pSinREP5iAchSnFR载体简介
pSinREP5iAchSnFR载体序列
LOCUS pSinREP5-iAchSnFR 11844 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Acetylcholine sensor for sindbis virus. ACCESSION . VERSION . KEYWORDS pSinREP5-iAchSnFR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11844) TITLE A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms and flies JOURNAL Unpublished REFERENCE 2 (bases 1 to 11844) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11844 /organism="synthetic DNA construct" /mol_type="other DNA" sig_peptide 1..63 /product="leader sequence from mouse immunoglobulin kappa light chain" /label=Ig-kappa leader primer_bind 513..534 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(637..658) /label=EGFP-N /note="EGFP, reverse primer" CDS 1645..1674 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 1678..1824 /codon_start=1 /product="transmembrane domain from platelet derived growth factor receptor beta" /label=PDGFR-beta TM domain /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ KKPR" promoter 2307..2411 /gene="bla" /label=AmpR promoter CDS 2412..3272 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2630..2649) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3443..4031 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3932..3951 /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter 4119..4137 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 4119..4136 /label=SP6 /note="SP6 promoter, forward primer" ORIGIN 1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt 61 gacagatctg cgaacgacac cgtagttgtg ggctcgatca tctttacaga agggattatc 121 gtcgcaaaca tggtggcaga gatgattgag gcgcatacag accttaaggt ggttcgcaaa 181 ctgaaccttg gcggggtcaa cgttaacttt gaagccatta aacgcggagg tgcgaataat 241 ggtattgaca tttacgtgga gtacactggg cacggtcttg tggatattct ggggttcccg 301 gagccgaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc gaacttcaag 361 atccgccaca acgtggagga cggcagcgtg cagctcgccg accactacca gcagaacacc 421 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgtg 481 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc 541 gccgggatca ctctcggcat ggacgagctg tacaagggcg gtaccggagg gagcatgagc 601 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta 661 aacggccaca agttcagcgt gcgcggcgag ggcgagggcg atgccaccaa cggcaagctg 721 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 781 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac 841 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatcag cttcaaggac 901 gacggcacct acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 961 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 1021 tacaactttc cgccgcccgc gactactgat ccagaaggtg catacgaaac cgtgaagaag 1081 gagtacaaac gtaaatggaa tattgtatgg ctcaaaccac tgggattcaa caatacgtat 1141 acgcttaccg ttaaagacga actggcgaaa cagtataacc ttaaaacctt cagtgactta 1201 gcgaaaatct cggataagct gattctgggt gcaacgatgt tctttttaga agggcccgat 1261 ggttacccag gcctgcaaaa actgtacaat ttcaaattca agcacaccaa aagcatggac 1321 atgggtattc gctataccgc cattgataat aacgaagttc aggtaattga tgcctgggcc 1381 actgatggct tgctggtgag ccacaaatta aaaattctgg aggatgataa agcgttcttc 1441 ccgccgtatt atgctgcccc catcatccgt caggatgtct tagataagca tcctgaactg 1501 aaggacgtgc tgaacaaact cgcgaatcaa atttcactgg aagaaatgca gaaactgaat 1561 tacaaggtgg acggtgaggg tcaggaccca gcgaaagtag ctaaggagtt tttgaaagag 1621 aaaggtttaa ttctgcaggt cgacgaacaa aaactcatct cagaagagga tctgaatgct 1681 gtgggccagg acacgcagga ggtcatcgtg gtgccacact ccttgccctt taaggtggtg 1741 gtgatctcag ccatcctggc cctggtggtg ctcaccatca tctcccttat catcctcatc 1801 atgctttggc agaagaagcc acgttaggcg gcgtgagcat gcaggccttg ggcccaatga 1861 tccgaccagc aaaactcgat gtacttccga ggaactgatg tgcataatgc atcaggctgg 1921 tacattagat ccccgcttac cgcgggcaat atagcaacac taaaaactcg atgtacttcc 1981 gaggaagcgc agtgcataat gctgcgcagt gttgccacat aaccactata ttaaccattt 2041 atctagcgga cgccaaaaac tcaatgtatt tctgaggaag cgtggtgcat aatgccacgc 2101 agcgtctgca taacttttat tatttctttt attaatcaac aaaattttgt ttttaacatt 2161 tcaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaagg ggaattcctc gataattaag 2221 cggccgctcg agcggccgct taattaatcg aggggaatta attcttgaag acgaaagggc 2281 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 2341 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 2401 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 2461 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 2521 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 2581 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 2641 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc 2701 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 2761 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 2821 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 2881 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 2941 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 3001 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 3061 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 3121 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 3181 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 3241 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 3301 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 3361 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 3421 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 3481 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 3541 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 3601 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 3661 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 3721 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 3781 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 3841 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 3901 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 3961 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 4021 gcctatggaa aaacgccagc aacgcgagct cgtatggaca tattgtcgtt agaacgcggc 4081 tacaattaat acataacctt atgtatcata cacatacgat ttaggtgaca ctatagattg 4141 acggcgtagt acacactatt gaatcaaaca gccgaccaat tgcactacca tcacaatgga 4201 gaagccagta gtaaacgtag acgtagaccc ccagagtccg tttgtcgtgc aactgcaaaa 4261 aagcttcccg caatttgagg tagtagcaca gcaggtcact ccaaatgacc atgctaatgc 4321 cagagcattt tcgcatctgg ccagtaaact aatcgagctg gaggttccta ccacagcgac 4381 gatcttggac ataggcagcg caccggctcg tagaatgttt tccgagcacc agtatcattg 4441 tgtctgcccc atgcgtagtc cagaagaccc ggaccgcatg atgaaatatg ccagtaaact 4501 ggcggaaaaa gcgtgcaaga ttacaaacaa gaacttgcat gagaagatta aggatctccg 4561 gaccgtactt gatacgccgg atgctgaaac accatcgctc tgctttcaca acgatgttac 4621 ctgcaacatg cgtgccgaat attccgtcat gcaggacgtg tatatcaacg ctcccggaac 4681 tatctatcat caggctatga aaggcgtgcg gaccctgtac tggattggct tcgacaccac 4741 ccagttcatg ttctcggcta tggcaggttc gtaccctgcg tacaacacca actgggccga 4801 cgagaaagtc cttgaagcgc gtaacatcgg actttgcagc acaaagctga gtgaaggtag 4861 gacaggaaaa ttgtcgataa tgaggaagaa ggagttgaag cccgggtcgc gggtttattt 4921 ctccgtagga tcgacacttt atccagaaca cagagccagc ttgcagagct ggcatcttcc 4981 atcggtgttc cacttgaatg gaaagcagtc gtacacttgc cgctgtgata cagtggtgag 5041 ttgcgaaggc tacgtagtga agaaaatcac catcagtccc gggatcacgg gagaaaccgt 5101 gggatacgcg gttacacaca atagcgaggg cttcttgcta tgcaaagtta ctgacacagt 5161 aaaaggagaa cgggtatcgt tccctgtgtg cacgtacatc ccggccacca tatgcgatca 5221 gatgactggt ataatggcca cggatatatc acctgacgat gcacaaaaac ttctggttgg 5281 gctcaaccag cgaattgtca ttaacggtag gactaacagg aacaccaaca ccatgcaaaa 5341 ttaccttctg ccgatcatag cacaagggtt cagcaaatgg gctaaggagc gcaaggatga 5401 tcttgataac gagaaaatgc tgggtactag agaacgcaag cttacgtatg gctgcttgtg 5461 ggcgtttcgc actaagaaag tacattcgtt ttatcgccca cctggaacgc agacctgcgt 5521 aaaagtccca gcctctttta gcgcttttcc catgtcgtcc gtatggacga cctctttgcc 5581 catgtcgctg aggcagaaat tgaaactggc attgcaacca aagaaggagg aaaaactgct 5641 gcaggtctcg gaggaattag tcatggaggc caaggctgct tttgaggatg ctcaggagga 5701 agccagagcg gagaagctcc gagaagcact tccaccatta gtggcagaca aaggcatcga 5761 ggcagccgca gaagttgtct gcgaagtgga ggggctccag gcggacatcg gagcagcatt 5821 agttgaaacc ccgcgcggtc acgtaaggat aatacctcaa gcaaatgacc gtatgatcgg 5881 acagtatatc gttgtctcgc caaactctgt gctgaagaat gccaaactcg caccagcgca 5941 cccgctagca gatcaggtta agatcataac acactccgga agatcaggaa ggtacgcggt 6001 cgaaccatac gacgctaaag tactgatgcc agcaggaggt gccgtaccat ggccagaatt 6061 cctagcactg agtgagagcg ccacgttagt gtacaacgaa agagagtttg tgaaccgcaa 6121 actataccac attgccatgc atggccccgc caagaataca gaagaggagc agtacaaggt 6181 tacaaaggca gagcttgcag aaacagagta cgtgtttgac gtggacaaga agcgttgcgt 6241 taagaaggaa gaagcctcag gtctggtcct ctcgggagaa ctgaccaacc ctccctatca 6301 tgagctagct ctggagggac tgaagacccg acctgcggtc ccgtacaagg tcgaaacaat 6361 aggagtgata ggcacaccgg ggtcgggcaa gtcagctatt atcaagtcaa ctgtcacggc 6421 acgagatctt gttaccagcg gaaagaaaga aaattgtcgc gaaattgagg ccgacgtgct 6481 aagactgagg ggtatgcaga ttacgtcgaa gacagtagat tcggttatgc tcaacggatg 6541 ccacaaagcc gtagaagtgc tgtacgttga cgaagcgttc gcgtgccacg caggagcact 6601 acttgccttg attgctatcg tcaggccccg caagaaggta gtactatgcg gagaccccat 6661 gcaatgcgga ttcttcaaca tgatgcaact aaaggtacat ttcaatcacc ctgaaaaaga 6721 catatgcacc aagacattct acaagtatat ctcccggcgt tgcacacagc cagttacagc 6781 tattgtatcg acactgcatt acgatggaaa gatgaaaacc acgaacccgt gcaagaagaa 6841 cattgaaatc gatattacag gggccacaaa gccgaagcca ggggatatca tcctgacatg 6901 tttccgcggg tgggttaagc aattgcaaat cgactatccc ggacatgaag taatgacagc 6961 cgcggcctca caagggctaa ccagaaaagg agtgtatgcc gtccggcaaa aagtcaatga 7021 aaacccactg tacgcgatca catcagagca tgtgaacgtg ttgctcaccc gcactgagga 7081 caggctagtg tggaaaacct tgcagggcga cccatggatt aagcagctca ctaacatacc 7141 taaaggaaac tttcaggcta ctatagagga ctgggaagct gaacacaagg gaataattgc 7201 tgcaataaac agccccactc cccgtgccaa tccgttcagc tgcaagacca acgtttgctg 7261 ggcgaaagca ttggaaccga tactagccac ggccggtatc gtacttaccg gttgccagtg 7321 gagcgaactg ttcccacagt ttgcggatga caaaccacat tcggccattt acgccttaga 7381 cgtaatttgc attaagtttt tcggcatgga cttgacaagc ggactgtttt ctaaacagag 7441 catcccacta acgtaccatc ccgccgattc agcgaggccg gtagctcatt gggacaacag 7501 cccaggaacc cgcaagtatg ggtacgatca cgccattgcc gccgaactct cccgtagatt 7561 tccggtgttc cagctagctg ggaagggcac acaacttgat ttgcagacgg ggagaaccag 7621 agttatctct gcacagcata acctggtccc ggtgaaccgc aatcttcctc acgccttagt 7681 ccccgagtac aaggagaagc aacccggccc ggtcgaaaaa ttcttgaacc agttcaaaca 7741 ccactcagta cttgtggtat cagaggaaaa aattgaagct ccccgtaaga gaatcgaatg 7801 gatcgccccg attggcatag ccggtgcaga taagaactac aacctggctt tcgggtttcc 7861 gccgcaggca cggtacgacc tggtgttcat caacattgga actaaataca gaaaccacca 7921 ctttcagcag tgcgaagacc atgcggcgac cttaaaaacc ctttcgcgtt cggccctgaa 7981 ttgccttaac ccaggaggca ccctcgtggt gaagtcctat ggctacgccg accgcaacag 8041 tgaggacgta gtcaccgctc ttgccagaaa gtttgtcagg gtgtctgcag cgagaccaga 8101 ttgtgtctca agcaatacag aaatgtacct gattttccga caactagaca acagccgtac 8161 acggcaattc accccgcacc atctgaattg cgtgatttcg tccgtgtatg agggtacaag 8221 agatggagtt ggagccgcgc cgtcataccg caccaaaagg gagaatattg ctgactgtca 8281 agaggaagca gttgtcaacg cagccaatcc gctgggtaga ccaggcgaag gagtctgccg 8341 tgccatctat aaacgttggc cgaccagttt taccgattca gccacggaga caggcaccgc 8401 aagaatgact gtgtgcctag gaaagaaagt gatccacgcg gtcggccctg atttccggaa 8461 gcacccagaa gcagaagcct tgaaattgct acaaaacgcc taccatgcag tggcagactt 8521 agtaaatgaa cataacatca agtctgtcgc cattccactg ctatctacag gcatttacgc 8581 agccggaaaa gaccgccttg aagtatcact taactgcttg acaaccgcgc tagacagaac 8641 tgacgcggac gtaaccatct attgcctgga taagaagtgg aaggaaagaa tcgacgcggc 8701 actccaactt aaggagtctg taacagagct gaaggatgaa gatatggaga tcgacgatga 8761 gttagtatgg attcatccag acagttgctt gaagggaaga aagggattca gtactacaaa 8821 aggaaaattg tattcgtact tcgaaggcac caaattccat caagcagcaa aagacatggc 8881 ggagataaag gtcctgttcc ctaatgacca ggaaagtaat gaacaactgt gtgcctacat 8941 attgggtgag accatggaag caatccgcga aaagtgcccg gtcgaccata acccgtcgtc 9001 tagcccgccc aaaacgttgc cgtgcctttg catgtatgcc atgacgccag aaagggtcca 9061 cagacttaga agcaataacg tcaaagaagt tacagtatgc tcctccaccc cccttcctaa 9121 gcacaaaatt aagaatgttc agaaggttca gtgcacgaaa gtagtcctgt ttaatccgca 9181 cactcccgca ttcgttcccg cccgtaagta catagaagtg ccagaacagc ctaccgctcc 9241 tcctgcacag gccgaggagg cccccgaagt tgtagcgaca ccgtcaccat ctacagctga 9301 taacacctcg cttgatgtca cagacatctc actggatatg gatgacagta gcgaaggctc 9361 acttttttcg agctttagcg gatcggacaa ctctattact agtatggaca gttggtcgtc 9421 aggacctagt tcactagaga tagtagaccg aaggcaggtg gtggtggctg acgttcatgc 9481 cgtccaagag cctgccccta ttccaccgcc aaggctaaag aagatggccc gcctggcagc 9541 ggcaagaaaa gagcccactc caccggcaag caatagctct gagtccctcc acctctcttt 9601 tggtggggta tccatgtccc tcggatcaat tttcgacgga gagacggccc gccaggcagc 9661 ggtacaaccc ctggcaacag gccccacgga tgtgcctatg tctttcggat cgttttccga 9721 cggagagatt gatgagctga gccgcagagt aactgagtcc gaacccgtcc tgtttggatc 9781 atttgaaccg ggcgaagtga actcaattat atcgtcccga tcagccgtat cttttcctct 9841 acgcaagcag agacgtagac gcaggagcag gaggactgaa tactgactaa ccggggtagg 9901 tgggtacata ttttcgacgg acacaggccc tgggcacttg caaaagaagt ccgttctgca 9961 gaaccagctt acagaaccga ccttggagcg caatgtcctg gaaagaattc atgccccggt 10021 gctcgacacg tcgaaagagg aacaactcaa actcaggtac cagatgatgc ccaccgaagc 10081 caacaaaagt aggtaccagt ctcgtaaagt agaaaatcag aaagccataa ccactgagcg 10141 actactgtca ggactacgac tgtataactc tgccacagat cagccagaat gctataagat 10201 cacctatccg aaaccattgt actccagtag cgtaccggcg aactactccg atccacagtt 10261 cgctgtagct gtctgtaaca actatctgca tgagaactat ccgacagtag catcttatca 10321 gattactgac gagtacgatg cttacttgga tatggtagac gggacagtcg cctgcctgga 10381 tactgcaacc ttctgccccg ctaagcttag aagttacccg aaaaaacatg agtatagagc 10441 cccgaatatc cgcagtgcgg ttccatcagc gatgcagaac acgctacaaa atgtgctcat 10501 tgccgcaact aaaagaaatt gcaacgtcac gcagatgcgt gaactgccaa cactggactc 10561 agcgacattc aatgtcgaat gctttcgaaa atatgcatgt aatgacgagt attgggagga 10621 gttcgctcgg aagccaatta ggattaccac tgagtttgtc accgcatatg tagctagact 10681 gaaaggccct aaggccgccg cactatttgc aaagacgtat aatttggtcc cattgcaaga 10741 agtgcctatg gatagattcg tcatggacat gaaaagagac gtgaaagtta caccaggcac 10801 gaaacacaca gaagaaagac cgaaagtaca agtgatacaa gccgcagaac ccctggcgac 10861 tgcttactta tgcgggattc accgggaatt agtgcgtagg cttacggccg tcttgcttcc 10921 aaacattcac acgctttttg acatgtcggc ggaggatttt gatgcaatca tagcagaaca 10981 cttcaagcaa ggcgacccgg tactggagac ggatatcgca tcattcgaca aaagccaaga 11041 cgacgctatg gcgttaaccg gtctgatgat cttggaggac ctgggtgtgg atcaaccact 11101 actcgacttg atcgagtgcg cctttggaga aatatcatcc acccatctac ctacgggtac 11161 tcgttttaaa ttcggggcga tgatgaaatc cggaatgttc ctcacacttt ttgtcaacac 11221 agttttgaat gtcgttatcg ccagcagagt actagaagag cggcttaaaa cgtccagatg 11281 tgcagcgttc attggcgacg acaacatcat acatggagta gtatctgaca aagaaatggc 11341 tgagaggtgc gccacctggc tcaacatgga ggttaagatc atcgacgcag tcatcggtga 11401 gagaccacct tacttctgcg gcggatttat cttgcaagat tcggttactt ccacagcgtg 11461 ccgcgtggcg gatcccctga aaaggctgtt taagttgggt aaaccgctcc cagccgacga 11521 cgagcaagac gaagacagaa gacgcgctct gctagatgaa acaaaggcgt ggtttagagt 11581 aggtataaca ggcactttag cagtggccgt gacgacccgg tatgaggtag acaatattac 11641 acctgtccta ctggcattga gaacttttgc ccagagcaaa agagcattcc aagccatcag 11701 aggggaaata aagcatctct acggtggtcc taaatagtca gcatagtaca tttcatctga 11761 ctaataccac aacaccacca cctctagcgc taccggactc agatctcgag ctcaagcttc 11821 gaattcggct tggggatatc cacc //
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pSinREP5iAchSnFR载体质粒应用举例
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