| 出品公司: | SIGMA |
|---|---|
| 载体名称: | pFLAG-CMV-2 |
| 质粒类型: | 哺乳动物表达载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 克隆方法: | 多克隆位点；限制性内切酶 |
| 启动子: | CMV |
| 载体大小: | 4679 bp |
| 5' 测序引物及序列: | CMV Forward：CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | hGH-pA-R：CCAGCTTGGTTCCCAATAGA |
| 载体标签: | FLAG tag (N-末端） |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 无 |
| 克隆菌株: | -- |
| 宿主细胞（系）: | 常用细胞系，如293、Hela等 |
| 备注: |
CMV启动子驱动目的蛋白高 水平表达； FLAG标签含8个氨基酸残基（DYKDDDDK）， 一般位于融合蛋白的外表面，具有高度免疫 灵敏性，便于检测和纯化目的蛋白； |
| 产品目录号: | E7398 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pFLAG-CMV-2载体质粒基本信息
pFLAG-CMV-2质粒图谱载体图谱和pFLAG-CMV-2载体序列质粒序列多克隆位点信息
pFLAG-CMV-2质粒载体简介
pFLAG-CMV-2质粒序列载体序列
LOCUS pFLAG-CMV-2 4679 bp DNA SYN
DEFINITION pFLAG-CMV-2
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4679
/organism="pFLAG-CMV-2"
/mol_type="other DNA"
misc_feature complement(12..155)
/label="lacZ_a"
misc_feature 126..148
/label="M13_pUC_fwd_primer"
promoter 141..157
/label="M13_forward20_primer"
promoter 319..895
/label="CMV_immearly_promoter"
misc_feature 398..685
/label="CAG_enhancer"
promoter 841..912
/label="CMV_promoter"
misc_feature 852..872
/label="CMV_fwd_primer"
misc_feature 896..915
/label="pCEP_fwd_primer"
terminator 1030..1496
/label="hGH_PA_terminator"
misc_feature complement(1666..1686)
/label="pBABE_3_primer"
misc_feature complement(1672..1887)
/label="SV40_enhancer"
promoter 1684..1952
/label="SV40_promoter"
rep_origin 1851..1928
/label="SV40_origin"
misc_feature 1913..1932
/label="SV40pro_F_primer"
promoter complement(2039..2057)
/label="T7_promoter"
misc_feature complement(2084..2106)
/label="M13_pUC_rev_primer"
promoter complement(2120..2149)
/label="lac_promoter"
gene complement(3232..4092)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(3232..4092)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(4134..4162)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 4241..4547
/label="f1_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
901 tcgtttagtg aaccgtcaga attgatctac catggactac aaagacgatg acgacaagct
961 tgcggccgcg aattcatcga tagatctgat atcggtacca gtcgactcta gaggatcccg
1021 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
1081 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
1141 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa
1201 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
1261 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
1321 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
1381 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
1441 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga
1501 ttttaaaata actataccag caggaggacg tccagacaca gcataggcta cctggccatg
1561 cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc gttgggtcca
1621 ctcagtagat gcctgttgaa ttgggtacgc ggccagcttg gctgtggaat gtgtgtcagt
1681 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
1741 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
1801 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
1861 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
1921 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
1981 gaggcctagg cttttgcaaa aagctcctcg aggaactgaa aaaccagaaa gttaattccc
2041 tatagtgagt cgtattaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
2101 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
2161 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
2221 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
2281 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
2341 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
2401 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
2461 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
2521 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
2581 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
2641 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
2701 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
2761 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
2821 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
2881 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
2941 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
3001 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
3061 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
3121 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
3181 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
3241 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
3301 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
3361 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
3421 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
3481 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
3541 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
3601 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
3661 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
3721 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
3781 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
3841 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
3901 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
3961 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
4021 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
4081 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
4141 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
4201 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
4261 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
4321 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
4381 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
4441 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
4501 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
4561 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
4621 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattt
//
pFLAG-CMV-2载体图谱质粒图谱pdf版和pFLAG-CMV-2载体序列质粒序列等相关资料下载
pFLAG-CMV-2质粒载体应用举例
- 上一篇：pFLAG-CMV-3
- 下一篇：pcDNA3.3-TOPO
