pGWB18

LOCUS       pGWB18               17409 bp    DNA     circular SYN 16-AUG-2007
DEFINITION  Gateway binary vector pGWB18 DNA, complete sequence.
ACCESSION   AB289781
VERSION     AB289781.1
KEYWORDS    .
SOURCE      Gateway binary vector pGWB18
  ORGANISM  Gateway binary vector pGWB18
            other sequences; artificial sequences; vectors.
REFERENCE   1
  AUTHORS   Nakagawa,T., Kurose,T., Hino,T., Tanaka,K., Kawamukai,M., Niwa,Y.,
            Toyooka,K., Matsuoka,K., Jinbo,T. and Kimura,T.
  TITLE     Development of series of gateway binary vectors, pGWBs, for
            realizing efficient construction of fusion genes for plant
            transformation
  JOURNAL   J. Biosci. Bioeng. 104 (1), 34-41 (2007)
   PUBMED   17697981
REFERENCE   2  (bases 1 to 17409)
  AUTHORS   Nakagawa,T. and Kimura,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2007) Tsuyoshi Nakagawa, Shimane University,
            Center for Integrated Research in Science; 1060 Nishikawatsu,
            Matsue, Shimane 690-8504, Japan
            (E-mail:tnakagaw@life.shimane-u.ac.jp, Tel:81-852-32-6595,
            Fax:81-852-32-6109)
COMMENT     constructed using pBI121.
FEATURES             Location/Qualifiers
     source          1..17409
                     /organism="Gateway binary vector pGWB18"
                     /mol_type="other DNA"
                     /db_xref="taxon:419562"
     misc_feature    complement(2454..2478)
                     /note="T-DNA right border"
     regulatory      2519..2825
                     /regulatory_class="promoter"
                     /note="NOS promoter"
     gene            2838..3632
                     /gene="nptII"
     CDS             2838..3632
                     /gene="nptII"
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase II"
                     /protein_id="BAF45017.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      4022..4277
                     /regulatory_class="terminator"
                     /note="NOS terminator"
     regulatory      4974..5808
                     /regulatory_class="promoter"
                     /note="35S promoter"
     misc_feature    5827..5997
                     /note="4xMyc"
     misc_feature    complement(6007..6131)
                     /note="attR1"
     gene            6252..6911
                     /gene="cat"
     CDS             6252..6911
                     /gene="cat"
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="BAF45018.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            7253..7558
                     /gene="ccdB"
     CDS             7253..7558
                     /gene="ccdB"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="BAF45019.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     misc_feature    7599..7723
                     /note="attR2"
     regulatory      7749..8001
                     /regulatory_class="terminator"
                     /note="NOS terminator"
     regulatory      complement(8430..8682)
                     /regulatory_class="terminator"
                     /note="NOS terminator"
     gene            complement(9150..10175)
                     /gene="hpt"
     CDS             complement(9150..10175)
                     /gene="hpt"
                     /codon_start=1
                     /transl_table=11
                     /product="hygromycin phosphotransferase"
                     /protein_id="BAF45020.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE"
     regulatory      complement(10179..10617)
                     /regulatory_class="promoter"
                     /note="35S promoter"
     misc_feature    complement(11272..11297)
                     /note="T-DNA left border"
ORIGIN      
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 atgagcgggt taattaacgg tgaacaaaag ctaatctccg aggaagactt gaacggtgaa
     5881 caaaaattaa tctcagaaga agacttgaac ggactcgacg gtgaacaaaa gttgatttct
     5941 gaagaagatt tgaacggtga acaaaagcta atctccgagg aagacttgaa cggtagcgct
     6001 gttatcacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
     6061 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
     6121 cagtcactat ggcggcccct cgaggggccg cattaggcac cccaggcttt acactttatg
     6181 cttccggctc gtataatgtg tggattttga gttaggatcc ggcgagattt tcaggagcta
     6241 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     6301 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     6361 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     6421 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
     6481 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     6541 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     6601 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     6661 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     6721 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     6781 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg
     6841 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     6901 gcggggcgta aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc
     6961 gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa
     7021 gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct
     7081 caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc
     7141 cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc
     7201 cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg aaatgcagtt
     7261 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga
     7321 tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc
     7381 agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat
     7441 gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct
     7501 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat
     7561 gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg
     7621 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
     7681 tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtgataacc tagagctcga
     7741 atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     7801 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     7861 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     7921 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     7981 tgtcatctat gttactagat cgggaattag cttcatcaac gcaagacatg cgcacgaccg
     8041 tctgacagga gaggaatttc cgacgagcac agaaaggact tgctcttgga cgtaggccta
     8101 tttctcaggc acatgtatca agtgttcgga cgtgggtttt cgatggtgta tcagccgccg
     8161 ccaactggga gatgaggagg ctttcttggg gggcagtcag cagttcattt cacaagacag
     8221 aggaacttgt aaggagatgc actgatttat cttggcgcaa accagcagga cgaattagtg
     8281 ggaatagccc gcgaatatct aagttatgcc tgtcggcatg agcagaaact tccaattcga
     8341 aacagtttgg agaggttgtt tttgggcata ccttttgtta gtcagcctct cgattgctca
     8401 tcgtcattac acagtaccga agtttgatcg atctagtaac atagatgaca ccgcgcgcga
     8461 taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg
     8521 cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat
     8581 tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg
     8641 attcaatctt aagaaacttt attgccaaat gtttgaacga tctgcttcga cgcactcctt
     8701 ctttactcca ccatctcgtc cttattgaaa acgtgggtag caccaaaacg aatcaagtcg
     8761 ctggaactga agttaccaat cacgctggat gatttgccag ttggattaat cttgcctttc
     8821 cccgcatgaa taatattgat gaatgcatgc gtgaggggta tttcgatttt ggcaatagct
     8881 gcaattgccg cgacatcctc caacgagcat aattcttcag aaaaatagcg atgttccatg
     8941 ttgtcagggc atgcatgatg cacgttatga ggtgacggtg ctaggcagta ttccctcaaa
     9001 gtttcatagt cagtatcata ttcatcattg cattcctgca agagagaatt gagacgcaat
     9061 ccacacgctg cggcaacctt ccggcgttcg tggtctattt gctcttggac gttgcaaacg
     9121 taagtgttgg atcccggtcg gcatctactc tattcctttg ccctcggacg agtgctgggg
     9181 cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt
     9241 ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat
     9301 cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg
     9361 tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc
     9421 ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag
     9481 atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct
     9541 gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc
     9601 cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac
     9661 gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg
     9721 catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac
     9781 ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc
     9841 agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg
     9901 gagatgcaat aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg
     9961 agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag
    10021 ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct
    10081 tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc
    10141 tcgacagacg tcgcggtgag ttcaggcttt ttcatatcgg ggtcgtcctc tccaaatgaa
    10201 atgaacttcc ttatatagag gaagggtctt gcgaaggata gtgggattgt gcgtcatccc
    10261 ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt
    10321 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg
    10381 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct
    10441 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc
    10501 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt
    10561 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttgacggat ctctaggacg
    10621 cgtcctagaa gctaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
    10681 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
    10741 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg cccgctcctt
    10801 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
    10861 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
    10921 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
    10981 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
    11041 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggaa ccaccatcaa
    11101 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
    11161 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccacccc
    11221 agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac
    11281 cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat
    11341 caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa
    11401 ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg
    11461 gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt
    11521 tgctgcctgt gatcaaatat catctccctc gcagagatcc gaattatcag ccttcttatt
    11581 catttctcgc ttaaccgtga caggctgtcg atcttgagaa ctatgccgac ataataggaa
    11641 atcgctggat aaagccgctg aggaagctga gtggcgctat ttctttagaa gtgaacgttg
    11701 acgatatcaa ctcccctatc cattgctcac cgaatggtac aggtcgggga cccgaagttc
    11761 cgactgtcgg cctgatgcat ccccggctga tcgaccccag atctggggct gagaaagccc
    11821 agtaaggaaa caactgtagg ttcgagtcgc gagatccccc ggaaccaaag gaagtaggtt
    11881 aaacccgctc cgatcaggcc gagccacgcc aggccgagaa cattggttcc tgtaggcatc
    11941 gggattggcg gatcaaacac taaagctact ggaacgagca gaagtcctcc ggccgccagt
    12001 tgccaggcgg taaaggtgag cagaggcacg ggaggttgcc acttgcgggt cagcacggtt
    12061 ccgaacgcca tggaaaccgc ccccgccagg cccgctgcga cgccgacagg atctagcgct
    12121 gcgtttggtg tcaacaccaa cagcgccacg cccgcagttc cgcaaatagc ccccaggacc
    12181 gccatcaatc gtatcgggct acctagcaga gcggcagaga tgaacacgac catcagcggc
    12241 tgcacagcgc ctaccgtcgc cgcgaccccg cccggcaggc ggtagaccga aataaacaac
    12301 aagctccaga atagcgaaat attaagtgcg ccgaggatga agatgcgcat ccaccagatt
    12361 cccgttggaa tctgtcggac gatcatcacg agcaataaac ccgccggcaa cgcccgcagc
    12421 agcataccgg cgacccctcg gcctcgctgt tcgggctcca cgaaaacgcc ggacagatgc
    12481 gccttgtgag cgtccttggg gccgtcctcc tgtttgaaga ccgacagccc aatgatctcg
    12541 ccgtcgatgt aggcgccgaa tgccacggca tctcgcaacc gttcagcgaa cgcctccatg
    12601 ggctttttct cctcgtgctc gtaaacggac ccgaacatct ctggagcttt cttcagggcc
    12661 gacaatcgga tctcgcggaa atcctgcacg tcggccgctc caagccgtcg aatctgagcc
    12721 ttaatcacaa ttgtcaattt taatcctctg tttatcggca gttcgtagag cgcgccgtgc
    12781 gtcccgagcg atactgagcg aagcaagtgc gtcgagcagt gcccgcttgt tcctgaaatg
    12841 ccagtaaagc gctggctgct gaacccccag ccggaactga ccccacaagg ccctagcgtt
    12901 tgcaatgcac caggtcatca ttgacccagg cgtgttccac caggccgctg cctcgcaact
    12961 cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa tccgatccgc
    13021 acatgaggcg gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag ctgaaatagt
    13081 cgaacatccg tcgggccgtc ggcgacagct tgcggtactt ctcccatatg aatttcgtgt
    13141 agtggtcgcc agcaaacagc acgacgattt cctcgtcgat caggacctgg caacgggacg
    13201 ttttcttgcc acggtccagg acgcggaagc ggtgcagcag cgacaccgat tccaggtgcc
    13261 caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg cattcctcgg
    13321 ccttcgtgta ataccggcca ttgatcgacc agcccaggtc ctggcaaagc tcgtagaacg
    13381 tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta ctccaacacc tgctgccaca
    13441 ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc acgtccttgt
    13501 tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg ttgcgcgtgg
    13561 tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc ggccacggcg
    13621 caatatcgaa caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt ttcagcaacg
    13681 cggcctgctt ggcctcgctg acctgttttg ccaggtcctc gccggcggtt tttcgcttct
    13741 tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct gccgcctcct
    13801 gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc gggcagggca gggggagcca
    13861 gttgcacgct gtcgcgctcg atcttggccg tagcttgctg gaccatcgag ccgacggact
    13921 ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg gcatcctcgg
    13981 cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac gtccgattca
    14041 ttcaccctcc ttgcgggatt gccccgactc acgccggggc aatgtgccct tattcctgat
    14101 ttgacccgcc tggtgccttg gtgtccagat aatccacctt atcggcaatg aagtcggtcc
    14161 cgtagaccgt ctggccgtcc ttctcgtact tggtattccg aatcttgccc tgcacgaata
    14221 ccagcgaccc cttgcccaaa tacttgccgt gggcctcggc ctgagagcca aaacacttga
    14281 tgcggaagaa gtcggtgcgc tcctgcttgt cgccggcatc gttgcgccac atctaggtac
    14341 taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc
    14401 agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg
    14461 acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag
    14521 ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag
    14581 acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta
    14641 aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag
    14701 taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg
    14761 atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt
    14821 tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc
    14881 cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat
    14941 agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc
    15001 atttttaaat ataggttttc attttctccc accagcttat ataccttagc aggagacatt
    15061 ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt
    15121 ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag
    15181 aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat
    15241 accagaaaac agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg
    15301 agccgatttt gaaaccacaa ttatgggtga tgctgccaac ttactgattt agtgtatgat
    15361 ggtgtttttg aggtgctcca gtggcttctg tgtctatcag ctgtccctcc tgttcagcta
    15421 ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctat ctctgctctc
    15481 actgccgtaa aacatggcaa ctgcagttca cttacaccgc ttctcaaccc ggtacgcacc
    15541 agaaaatcat tgatatggcc atgaatggcg ttggatgccg ggcaacagcc cgcattatgg
    15601 gcgttggcct caacacgatt ttacgtcact taaaaaactc aggccgcagt cggtaacctc
    15661 gcgcatacag ccgggcagtg acgtcatcgt ctgcgcggaa atggacgaac agtggggcta
    15721 tgtcggggct aaatcgcgcc agcgctggct gttttacgcg tatgacagtc tccggaagac
    15781 ggttgttgcg cacgtattcg gtgaacgcac tatggcgacg ctggggcgtc ttatgagcct
    15841 gctgtcaccc tttgacgtgg tgatatggat gacggatggc tggccgctgt atgaatcccg
    15901 cctgaaggga aagctgcacg taatcagcaa gcgatatacg cagcgaattg agcggcataa
    15961 cctgaatctg aggcagcacc tggcacggct gggacggaag tcgctgtcgt tctcaaaatc
    16021 ggtggagctg catgacaaag tcatcgggca ttatctgaac ataaaacact atcaataagt
    16081 tggagtcatt acccaattat gatagaattt acaagctata aggttattgt cctgggtttc
    16141 aagcattagt ccatgcaagt ttttatgctt tgcccattct atagatatat tgataagcgc
    16201 gctgcctatg ccttgccccc tgaaatcctt acatacggcg atatcttcta tataaaagat
    16261 atattatctt atcagtattg tcaatatatt caaggcaatc tgcctcctca tcctcttcat
    16321 cctcttcgtc ttggtagctt tttaaatatg gcgcttcata gagtaattct gtaaaggtcc
    16381 aattctcgtt ttcatacctc ggtataatct tacctatcac ctcaaatggt tcgctgggtt
    16441 tatcgcaccc ccgaacacga gcacggcacc cgcgaccact atgccaagaa tgcccaaggt
    16501 aaaaattgcc ggccccgcca tgaagtccgt gaatgccccg acggccgaag tgaagggcag
    16561 gccgccaccc aggccgccgc cctcactgcc cggcacctgg tcgctgaatg tcgatgccag
    16621 cacctgcggc acgtcaatgc ttccgggcgt cgcgctcggg ctgatcgccc atcccgttac
    16681 tgccccgatc ccggcaatgg caaggactgc cagcgctgcc atttttgggg tgaggccgtt
    16741 cgcggccgag gggcgcagcc cctgggggga tgggaggccc gcgttagcgg gccgggaggg
    16801 ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct
    16861 ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca ggttagcggt
    16921 ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg gacagcccct
    16981 caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt gtcaaggatc
    17041 gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc
    17101 ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt
    17161 gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg
    17221 ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg
    17281 ccaagttttc cgcgaggtat ccacaacgcc ggcggccgcg gtgtctcgca cacggcttcg
    17341 acggcgtttc tggcgcgttt gcagggccat agacggccgc cagcccagcg gcgagggcaa
    17401 ccagcccgg
//