| 质粒名称: | FU-tet-o-hSox2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 19779 |
| 存储实验室: | Konrad Hochedlinger |
|
载体骨架基本信息 |
|
| 载体名称: | FUdeltaGW |
| 完整质粒大小: | 9217bp |
| 载体类型: | 哺乳细胞表达、 Lentiviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Sox2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 950bp |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoRI (unknown if destroyed) |
| 3’克隆位点: | EcoRI (unknown if destroyed) |
| 5’测序引物 | CMV-F |
|
文献引用方法 |
|
| 材料和方法部分: | FU-tet-o-hSox2 was a gift from Konrad Hochedlinger (Addgene plasmid # 19779 ; http://n2t.net/addgene:19779 ; RRID:Addgene_19779) |
| 参考文献部分: | A high-efficiency system for the generation and study of human induced pluripotent stem cells. Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger K. Cell Stem Cell. 2008 Sep 11. 3(3):340-5. 10.1016/j.stem.2008.08.003 PubMed 18786420 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS FU-tet-o-hSox2 9217 bp ds-DNA circular SYN 16-AUG-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS FU-tet-o-hSox2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9217)
AUTHORS Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger
K
TITLE A high-efficiency system for the generation and study of human
induced pluripotent stem cells.
JOURNAL Cell Stem Cell. 2008 Sep 11. 3(3):340-5.
PUBMED 18786420
REFERENCE 2 (bases 1 to 9217)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 16, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9217
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 772..792
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1680..1913
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2098..2142
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2440..2557
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 2610..2628
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2652..2670
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2694..2712
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2736..2754
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
promoter 2787..2825
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2822..2846
/label=LNCX
/note="Human CMV promoter, forward primer"
misc_feature 3909..4497
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3962..3982)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4380..4391)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(4500..4516)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4501..4517)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 5022..5202
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(5228..5245)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5234..5458
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5504..5932
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5591..5610)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5801..5822
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5941..5961)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5946..6275
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6126..6261
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6188..6207
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 6323..6370
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 6389..6763
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 6893..7014
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6930..6949)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6984..7003
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7063..7079)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7063..7079)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7076..7098)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7087..7103
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7111..7141)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7156..7177
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7294..7311)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7465..8053)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7545..7564)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8224..9084)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8847..8866
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9085..9189)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
2581 agatccagtt tggttatcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
2641 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
2701 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
2761 aaagtcgagc tcggtacccg ggtcgaggta ggcgtgtacg gtgggaggcc tatataagca
2821 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
2881 atagaagaca ccgggaccga tccagcctcc gcggccccga attcgccacc atgtacaaca
2941 tgatggagac ggagctgaag ccgccgggcc cgcagcaaac ttcggggggc ggcggcggca
3001 actccaccgc ggcggcggcc ggcggcaacc agaaaaacag cccggaccgc gtcaagcggc
3061 ccatgaatgc cttcatggtg tggtcccgcg ggcagcggcg caagatggcc caagagaacc
3121 ccaagatgca caactcggag atcagcaagc gcctgggcgc cgagtggaaa cttttgtcgg
3181 agacggagaa gcggccgttc atcgacgagg ctaagcggct gcgagcgctg cacatgaagg
3241 agcacccgga ttataaatac cggccccggc ggaaaaccaa gacgctcatg aagaaggata
3301 agtacacgct gcccggcggg ctgctggccc ccggcggcaa tagcatggcg agcggggtcg
3361 gggtgggcgc cggcctgggc gcgggcgtga accagcgcat ggacagttac gcgcacatga
3421 acggctggag caacggcagc tacagcatga tgcaggacca gctgggctac ccgcagcacc
3481 cgggcctcaa tgcgcacggc gcagcgcaga tgcagcccat gcaccgctac gacgtgagcg
3541 ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg cccacctaca
3601 gcatgtccta ctcgcagcag ggcacccctg gcatggctct tggctccatg ggttcggtgg
3661 tcaagtccga ggccagctcc agcccccctg tggttacctc ttcctcccac tccagggcgc
3721 cctgccaggc cggggacctc cgggacatga tcagcatgta tctccccggc gccgaggtgc
3781 cggaacccgc cgcccccagc agacttcaca tgtcccagca ctaccagagc ggcccggtgc
3841 ccggcacggc cattaacggc acactgcccc tctcacacat gtgagaattc gatatcaagc
3901 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
3961 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
4021 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
4081 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
4141 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
4201 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
4261 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
4321 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
4381 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
4441 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
4501 gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
4561 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
4621 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
4681 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc
4741 ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag
4801 ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
4861 aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc
4921 tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag
4981 catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
5041 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
5101 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
5161 gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc
5221 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
5281 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
5341 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
5401 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
5461 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
5521 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
5581 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
5641 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
5701 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
5761 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
5821 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
5881 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
5941 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
6001 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
6061 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
6121 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
6181 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
6241 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
6301 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
6361 ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct
6421 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
6481 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
6541 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
6601 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
6661 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
6721 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
6781 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
6841 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
6901 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
6961 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
7021 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
7081 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
7141 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
7201 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
7261 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
7321 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
7381 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
7441 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
7501 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
7561 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
7621 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
7681 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
7741 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
7801 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
7861 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
7921 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
7981 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
8041 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
8101 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
8161 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
8221 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
8281 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
8341 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
8401 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
8461 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
8521 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
8581 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
8641 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
8701 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
8761 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
8821 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
8881 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
8941 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
9001 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
9061 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
9121 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
9181 ggttccgcgc acatttcccc gaaaagtgcc acctgac
//
