| 质粒名称: | pGIPZ-PD-L1-EGFP |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 120933 |
| 存储实验室: | Mien-Chie Hung |
|
载体骨架基本信息 |
|
| 载体名称: | pGIPZ |
| 完整质粒大小: | 12666 bp |
| 载体类型: | 细菌细胞表达 |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | PD-L1-EGFP |
| 物种来源: | H. sapiens (human) |
|
文献引用方法 |
|
| 材料和方法部分: | pGIPZ-PD-L1-EGFP was a gift from Mien-Chie Hung (Addgene plasmid # 120933 ; http://n2t.net/addgene:120933 ; RRID:Addgene_120933) |
| 参考文献部分: | Exosomal PD-L1 harbors active defense function to suppress T cell killing of breast cancer cells and promote tumor growth. Yang Y, Li CW, Chan LC, Wei Y, Hsu JM, Xia W, Cha JH, Hou J, Hsu JL, Sun L, Hung MC. Cell Res. 2018 Aug;28(8):862-864. doi: 10.1038/s41422-018-0060-4. Epub 2018 Jun 29. 10.1038/s41422-018-0060-4 PubMed 29959401 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGIPZ-PD-L1-EGFP 12666 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Express PD-L1-EGFP fusion protein in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pGIPZ-PD-L1-EGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12666)
AUTHORS Yang Y, Li CW, Chan LC, Wei Y, Hsu JM, Xia W, Cha JH, Hou J, Hsu JL,
Sun L, Hung MC
TITLE Exosomal PD-L1 harbors active defense function to suppress T cell
killing of breast cancer cells and promote tumor growth.
JOURNAL Cell Res. 2018 Aug;28(8):862-864. doi: 10.1038/s41422-018-0060-4.
Epub 2018 Jun 29.
PUBMED 29959401
REFERENCE 2 (bases 1 to 12666)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..12666
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 155..788
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 835..960
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1457..1690
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1875..1919
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2217..2334
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
CDS complement(2399..2773)
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
regulatory 2770..2779
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
promoter complement(2783..2801)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(2793..2840)
/label=EM7 promoter
/note="synthetic bacterial promoter "
enhancer 2945..3248
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3249..3452
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 3403..3423
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 3449..3473
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 4465..4474
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4471..5190
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 4471..5187
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4516..4537)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4777..4796)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5124..5145
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 5226..5798
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(5393..5410)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 5620..5639
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 5801..6403
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind 6300..6320
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
ncRNA 6410..6531
/label=5' miR-30a
/note="sequence upstream of the 71-nt precursor of the
human miR-30a microRNA (Zeng et al., 2002)"
/ncRNA_class="miRNA"
ncRNA 6559..6573
/label=miR-30a loop
/note="loop from the the 71-nt precursor of the human
miR-30a microRNA (Zeng et al., 2002)"
/ncRNA_class="miRNA"
ncRNA 6601..6727
/label=3' miR-30a
/note="sequence downstream of the 71-nt precursor of the
human miR-30a microRNA (Zeng et al., 2002)"
/ncRNA_class="miRNA"
misc_feature 6795..7383
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6848..6868)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(7266..7277)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 7590..7823
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(7924..7941)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 7930..8154
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 8200..8628
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8287..8306)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8497..8518
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(8637..8657)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 8642..8971
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 8822..8957
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8884..8903
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 9020..10045
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
polyA_signal 10175..10296
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10345..10361)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10345..10361)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(10358..10380)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 10369..10385
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(10393..10423)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10438..10459
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(10576..10593)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(10747..11335)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10827..10846)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(11506..12366)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 12129..12148
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(12367..12471)
/gene="bla"
/label=AmpR promoter
polyA_signal 12519..12653
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
61 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
121 gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga
181 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
241 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
301 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
361 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
421 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
481 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
541 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
601 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
661 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
721 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
781 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
841 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
901 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
961 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
1021 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
1081 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
1141 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
1201 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
1261 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
1321 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
1381 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
1441 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
1501 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
1561 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
1621 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
1681 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
1741 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
1801 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
1861 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
1921 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
1981 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
2041 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
2101 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
2161 acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt
2221 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
2281 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
2341 tattacaggg acagcagaga tccagtttgg ttagtaccgg gcccgctcta gtccggaatc
2401 agtcctgctc ctcggccacg aagtgcacgc agttgccggc cgggtcgcgc agggcgaact
2461 cccgccccca cggctgctcg ccgatctcgg tcatggccgg cccggaggcg tcccggaagt
2521 tcgtggacac gacctccgac cactcggcgt acagctcgtc caggccgcgc acccacaccc
2581 aggccagggt gttgtccggc accacctggt cctggaccgc gctgatgaac agggtcacgt
2641 cgtcccggac cacaccggcg aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt
2701 cggtccagaa ctcgaccgct ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg
2761 tcaacttggc catggtggcc ctcctatagt gagtcgtatt atactatgcc gatatactat
2821 gccgatgatt aattgtcaac acgtgctgca ggtccgaggt tctagacgta ttaccgccat
2881 gcattagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
2941 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
3001 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
3061 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
3121 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
3181 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
3241 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
3301 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
3361 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
3421 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
3481 gacgccatcc acgctgtttt gacctccata gaagacaccg actctactag aggatctgcc
3541 accatcgata tgaggatatt tgctgtcttt atattcatga cctactggca tttgctgaac
3601 gcatttactg tcacggttcc caaggaccta tatgtggtag agtatggtag caatatgaca
3661 attgaatgca aattcccagt agaaaaacaa ttagacctgg ctgcactaat tgtctattgg
3721 gaaatggagg ataagaacat tattcaattt gtgcatggag aggaagacct gaaggttcag
3781 catagtagct acagacagag ggcccggctg ttgaaggacc agctctccct gggaaatgct
3841 gcacttcaga tcacagatgt gaaattgcag gatgcagggg tgtaccgctg catgatcagc
3901 tatggtggtg ccgactacaa gcgaattact gtgaaagtca atgccccata caacaaaatc
3961 aaccaaagaa ttttggttgt ggatccagtc acctctgaac atgaactgac atgtcaggct
4021 gagggctacc ccaaggccga agtcatctgg acaagcagtg accatcaagt cctgagtggt
4081 aagaccacca ccaccaattc caagagagag gagaagcttt tcaatgtgac cagcacactg
4141 agaatcaaca caacaactaa tgagattttc tactgcactt ttaggagatt agatcctgag
4201 gaaaaccata cagctgaatt ggtcatccca gaactacctc tggcacatcc tccaaatgaa
4261 aggactcact tggtaattct gggagccatc ttattatgcc ttggtgtagc actgacattc
4321 atcttccgtt taagaaaagg gagaatgatg gatgtgaaaa aatgtggcat ccaagataca
4381 aactcaaaga agcaaagtga tacacatttg gaggagacgg gaattctgca gtcgacggta
4441 ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
4501 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
4561 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
4621 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
4681 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
4741 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
4801 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
4861 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
4921 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
4981 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
5041 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
5101 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
5161 actctcggca tggacgagct gtacaagtaa gctagctaat gtacaagtag cggccgcaaa
5221 ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
5281 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
5341 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
5401 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
5461 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
5521 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
5581 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
5641 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
5701 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
5761 cacggggacg tggttttcct ttgaaaaaca cgataatacc atggccaccg agtacaagcc
5821 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc
5881 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt
5941 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
6001 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
6061 ggtgttcgcc gagatcggct cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
6121 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
6181 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
6241 cggagtggag gcggccgagc gcgctggggt gcccgccttc ctggagacct ccgcgccccg
6301 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
6361 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtttgtt tgaatgaggc
6421 ttcagtactt tacagaatcg ttgcctgcac atcttggaaa cacttgctgg gattacttct
6481 tcaggttaac ccaacagaag gctcgagaag gtatattgct gttgacagtg agcgcatgaa
6541 gaaagatgga gtcaaatagt gaagccacag atgtatttga ctccatcttt cttcatttgc
6601 ctactgcctc ggaattcaag gggctacttt aggagcaatt atcttgttta ctaaaactga
6661 ataccttgct atctctttga tacattttta caaagctgaa ttaaaatggt ataaattaaa
6721 tcactttttt ggccggccaa cttgcagtac accggaccac gaccctagga tcgacgcgga
6781 cacgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
6841 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
6901 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
6961 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
7021 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
7081 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
7141 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
7201 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
7261 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
7321 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
7381 cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa
7441 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
7501 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
7561 tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca
7621 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
7681 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
7741 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
7801 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
7861 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgtttaa acccgctgat
7921 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
7981 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
8041 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
8101 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
8161 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
8221 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
8281 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
8341 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
8401 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
8461 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
8521 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
8581 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
8641 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
8701 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
8761 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
8821 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
8881 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
8941 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
9001 cggatctgat cagcacgtga tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
9061 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
9121 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
9181 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
9241 tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg
9301 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
9361 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
9421 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
9481 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
9541 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
9601 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
9661 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
9721 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
9781 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
9841 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
9901 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
9961 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
10021 cactcgtccg agggcaaagg aatagcacgt gctacgagat ttcgattcca ccgccgcctt
10081 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
10141 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
10201 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
10261 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
10321 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
10381 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
10441 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
10501 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
10561 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
10621 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
10681 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
10741 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
10801 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
10861 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
10921 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
10981 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
11041 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
11101 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
11161 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
11221 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
11281 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
11341 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
11401 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
11461 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
11521 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
11581 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
11641 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
11701 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
11761 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
11821 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
11881 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
11941 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
12001 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
12061 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
12121 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
12181 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
12241 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
12301 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
12361 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
12421 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
12481 ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata
12541 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
12601 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
12661 taactc
//
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