| 出品公司: | CAMBIA |
|---|---|
| 载体名称: | pEarleyGate205； pEarleyGate 205 |
| 质粒类型: | Gateway载体；植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | Gateway |
| 启动子: | 35S |
| 载体大小: | 12188 bp |
| 5' 测序引物及序列: | 35S promoter：CTATCCTTCGCAAGACCCTTC |
| 3' 测序引物及序列: | -- |
| 载体标签: | 钙结合蛋白CBP、TEV site、2XPortA (C ter) |
| 载体抗性: | 卡那霉素、氯霉素（仅空载体） |
| 筛选标记: | 草丁膦（磷丝菌素）phosphinothricin、 |
| 克隆菌株: | DB3.1菌株 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Exported 12188 bp ds-DNA circular SYN 26-JUL-2016
DEFINITION Gateway(R)-compatible plant transformation vector with a C-terminal
TAP tag.
ACCESSION .
VERSION .
KEYWORDS pEarleyGate 205
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12188)
AUTHORS Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS.
TITLE Gateway-compatible vectors for plant functional genomics and
proteomics.
JOURNAL Plant J. 2006;45:616-29.
PUBMED 16441352
REFERENCE 2 (bases 1 to 12188)
AUTHORS Pikaard Lab
TITLE Direct Submission
JOURNAL Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4
http://www.snapgene.com
COMMENT The sequence was corrected by inserting a G at position 8296.
FEATURES Location/Qualifiers
source 1..12188
/organism="synthetic DNA construct"
/lab_host="Plant Cells"
/mol_type="other DNA"
misc_feature 1..25
/note="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA"
terminator 111..363
/note="MAS terminator"
/note="mannopine synthase terminator"
CDS complement(373..924)
/codon_start=1
/gene="Streptomyces hygroscopicus bar"
/product="phosphinothricin acetyltransferase草丁膦（磷丝菌素）
乙酰转移酶"
/note="BlpR"
/note="confers resistance to bialophos or phosphinothricin"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
WQLDFSLPVPPRPVLPVTEI"
promoter complement(930..1310)
/note="MAS promoter"
/note="mannopine synthase promoter (Velten et al., 1984)"
promoter 2306..2651
/note="35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
protein_bind 2663..2782
/gene="mutant version of attR"
/bound_moiety="LR Clonase(TM)"
/note="attR1"
/note="recombination site for the Gateway(R) LR reaction"
promoter 2812..2842
/note="lac UV5 promoter"
/note="E. coli lac promoter with an ""up"" mutation"
CDS 2896..3555
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
CDS 3897..4202
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/note="ccdB"
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
protein_bind complement(4243..4367)
/gene="mutant version of attR"
/bound_moiety="LR Clonase(TM)"
/note="attR2"
/note="recombination site for the Gateway(R) LR reaction"
CDS 4381..4458
/codon_start=1
/product="calmodulin-binding peptide"
/note="CBP"
/note="derived from skeletal muscle myosin light chain
kinase; binds calmodulin with nanomolar affinity in the
presence of calcium"
/translation="KRRWKKNFIAVSAANRFKKISSSGAL"
CDS 4486..4506
/codon_start=1
/product="tobacco etch virus (TEV) recognition and cleavage
site"
/note="TEV Site"
/translation="ENLYFQG"
CDS 4546..4719
/codon_start=1
/product="IgG-binding unit of Staphylococcus aureus protein
A"
/note="ProtA"
/translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
AEAKKLNDAQAPK"
CDS 4720..4893
/codon_start=1
/product="IgG-binding unit of Staphylococcus aureus protein
A"
/note="ProtA"
/translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
AEAKKLNGAQAPK"
terminator 4959..5666
/note="OCS terminator"
/note="octopine synthase terminator"
misc_feature 5910..5934
/note="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
CDS 7234..7863
/codon_start=1
/product="stability protein from Pseudomonas plasmid pVS1"
/note="pVS1 StaA"
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 8292..9365
/codon_start=1
/product="replication protein from Pseudomonas plasmid
pVS1"
/note="pVS1 RepA"
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 9431..9625
/note="pVS1 oriV"
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 9969..10109
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(10295..10883)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(10970..11764)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga
1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
2641 tggagaggac acgctcgaga tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
2701 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
2761 taaaacacaa catatccagt catattggcg gccgcattag gcaccccagg ctttacactt
2821 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
2881 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
2941 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
3001 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
3061 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
3121 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
3181 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
3241 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
3301 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
3361 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
3421 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
3481 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
3541 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
3601 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
3661 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
3721 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
3781 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
3841 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
3901 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
3961 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
4021 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
4081 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
4141 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
4201 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
4261 tgtgttttac agcattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
4321 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgcc taggatggaa
4381 aagagaagat ggaaaaagaa tttcatagcc gtctcagcag ccaaccgctt taagaaaatc
4441 tcatcctccg gggcacttga ttatgatatt ccaactactg ctagcgagaa tttgtatttt
4501 cagggtgagc tcaaaaccgc ggctcttgcg caacacgatg aagccgtgga caacaaattc
4561 aacaaagaac aacaaaacgc gttctatgag atcttacatt tacctaactt aaacgaagaa
4621 caacgaaacg ccttcatcca aagtttaaaa gatgacccaa gccaaagcgc taacctttta
4681 gcagaagcta aaaagctaaa tgatgctcag gcgccgaaag tagacaacaa attcaacaaa
4741 gaacaacaaa acgcgttcta tgagatctta catttaccta acttaaacga agaacaacga
4801 aacgccttca tccaaagttt aaaagatgac ccaagccaaa gcgctaacct tttagcagaa
4861 gctaaaaagc taaatggtgc tcaggcgccg aaagtagacg cgaattccgc ggggaagtca
4921 acctgattaa ttaagacccg ggactagtcc ctagagtcct gctttaatga gatatgcgag
4981 acgcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga
5041 gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg
5101 ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt accctactac
5161 ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat
5221 ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa
5281 aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc
5341 aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact
5401 gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg
5461 gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg
5521 taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat
5581 gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt
5641 tgcgacaaca tgtcgaggct cagcaggacc tgcaggcatg caagcttggc actggccgtc
5701 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
5761 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
5821 cagttgcgca gcctgaatgg cgaatgctag agcagcttga gcttggatca gattgtcgtt
5881 tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga
5941 gaaaagagcg tttattagaa taacggatat ttaaaagggc gtgaaaaggt ttatccgttc
6001 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc
6061 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa
6121 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc
6181 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac
6241 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg
6301 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt
6361 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc
6421 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc
6481 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg
6541 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg
6601 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg
6661 ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc
6721 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg
6781 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg
6841 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg
6901 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga
6961 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga
7021 gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc
7081 caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa
7141 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg
7201 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag
7261 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc
7321 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc
7381 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac
7441 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac
7501 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct
7561 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg
7621 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc
7681 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg
7741 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc
7801 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt
7861 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc
7921 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga
7981 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac
8041 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa
8101 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat
8161 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg
8221 ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc
8281 gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
8341 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
8401 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
8461 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
8521 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
8581 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
8641 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
8701 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
8761 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
8821 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
8881 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
8941 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
9001 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
9061 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
9121 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
9181 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
9241 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
9301 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
9361 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
9421 gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
9481 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
9541 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
9601 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
9661 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
9721 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
9781 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt
9841 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
9901 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
9961 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
10021 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
10081 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
10141 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
10201 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
10261 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
10321 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
10381 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
10441 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
10501 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
10561 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
10621 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
10681 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
10741 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
10801 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
10861 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
10921 agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt
10981 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
11041 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
11101 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
11161 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
11221 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
11281 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
11341 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
11401 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
11461 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
11521 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
11581 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
11641 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
11701 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
11761 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
11821 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
11881 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
11941 gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
12001 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
12061 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact
12121 gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg
12181 ctggctgg
//
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