| 出品人: | Ouahrani-Bettache,S., Porte,F. |
|---|---|
| 载体名称: | pBBR1-GFP |
| 质粒类型: | 广宿主载体；克隆载体；荧光报告载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lac |
| 载体大小: | 6640 bp |
| 5' 测序引物及序列: | M13 Forward 5’TGTAAAACGACGGCCAGT 3’ |
| 3' 测序引物及序列: | M13 Reverse 5’CAGGAAACAGCTATGAC 3’ |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 多个菌属 |
| 备注: | -- |
| 产品目录号: | -- |
| 组成型/诱导型: | 诱导型 |
买家导航
pBBR1-GFP载体质粒基本信息
pBBR1-GFP质粒图谱载体图谱和pBBR1-GFP载体序列质粒序列多克隆位点信息
pBBR1-GFP质粒载体简介
pBBR1-GFP质粒序列载体序列
LOCUS AF110459 6640 bp DNA circular SYN 29-APR-1999
DEFINITION Cloning vector pBBR1-GFP, complete sequence.
VERSION AF110459.1 GI:4704641
TITLE pBBR1-GFP: a broad-host-range vector for prokaryotic promoter
studies
AUTHORS Ouahrani-Bettache,S., Porte,F., Teyssier,J., Liautard,J.P. and
Koehler,S.
JOURNAL Submitted (02-DEC-1998) INSERM U-431, University of Montpellier II,
Pl. E. Bataillon, Montpellier 34095, France
FEATURES Location/Qualifiers
source 1..6640
/organism="Cloning vector pBBR1-GFP"
/mol_type="genomic DNA"
/db_xref="taxon:92689"
misc_feature 1..2642
/note="from pBBR1MCS: GenBank Accession Number U02374"
misc_feature 2686..4182
/note="from pSV2-Cat: GenBank Accession Number M77788"
gene complement(3491..4150)
/gene="cat"
CDS complement(3491..4150)
/gene="cat"
/codon_start=1
/transl_table=11
/product="chloramphenicol-acetyl-transferase (CAT)"
/protein_id="AAD28188.1"
/db_xref="GI:4704642"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
misc_feature complement(4183..4204)
/note="from pBBR1MCS: GenBank Accession Number U02374"
gene complement(4213..4929)
/gene="gfp"
/note="modified form mut3"
CDS complement(4213..4929)
/gene="gfp"
/note="GFP; modified form mut3; from Aequorea victoria
GFP: GenBank Accession Number M62653"
/codon_start=1
/transl_table=11
/product="green fluorescent protein"
/protein_id="AAD28189.1"
/db_xref="GI:4704643"
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
VLLEFVTAAGITHGMDELYK"
misc_feature 4983..4997
/note="from pBBR1MCS: GenBank Accession number U02374"
misc_feature 4998..6446
/note="from pUC18: GenBank Accession Number L08752"
gene complement(5569..6429)
/gene="bla"
CDS complement(5569..6429)
/gene="bla"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AAD28190.1"
/db_xref="GI:4704644"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 6447..6640
/note="from pBBR1MCS: GenBank Accession Number U02374"
ORIGIN
1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
2581 cctgaatgga agccccaccg cggtggcggc cgctctagaa ctagtggatc ccccgggctg
2641 caggaattcg atatcaagct tatcgatacc gtcgacctcg aggtcaacaa caacaattgc
2701 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
2761 ctctacaaat gtggtatggc tgattatgat ctctagtcaa ggcactatac atcaaatatt
2821 ccttattaac ccctttacaa attaaaaagc taaaggtaca caatttttga gcatagttat
2881 taatagcaga cactctatgc ctgtgtggag taagaaaaaa cagtatgtta tgattataac
2941 tgttatgcct acttataaag gttacagaat atttttccat aattttcttg tatagcagtg
3001 cagctttttc ctttgtggtg taaatagcaa agcaagcaag agttctatta ctaaacacag
3061 catgactcaa aaaacttagc aattctgaag gaaagtcctt ggggtcttct acctttctct
3121 tcttttttgg aggagtagaa tgttgagagt cagcagtagc ctcatcatca ctagatggca
3181 tttcttctga gcaaaacagg ttttcctcat taaaggcatt ccaccactgc tcccattcat
3241 cagttccata ggttggaatc taaaatacac aaacaattag aatcagtagt ttaacacatt
3301 atacacttaa aaattttata tttaccttag agctttaaat ctctgtaggt agtttgtcca
3361 attatgtcac accacagaag taaggttcct tcacaaagat ccggcgaatt tctgccattc
3421 atccgcttat tatcacttat tcaggcgtag caccaggcgt ttaagggcac caataactgc
3481 cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
3541 ttctgccgac atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca
3601 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
3661 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
3721 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
3781 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
3841 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
3901 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg
3961 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
4021 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
4081 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
4141 ttttctccat tttagcttcc ttagctcctg aaaatctcgc caagcttgat atcgaattcc
4201 tgcaggtctg gactatttgt atagttcatc catgccatgt gtaatcccag cagctgttac
4261 aaactcaaga aggaccatgt ggtctctctt ttcgttggga tctttcgaaa gggcagattg
4321 tgtggacagg taatggttgt ctggtaaaag gacagggcca tcgccaattg gagtattttg
4381 ttgataatgg tctgctagtt gaacgcttcc atcttcaatg ttgtgtctaa ttttgaagtt
4441 aactttgatt ccattctttt gtttgtctgc catgatgtat acattgtgtg agttatagtt
4501 gtattccaat ttgtgtccaa gaatgtttcc atcttcttta aaatcaatac cttttaactc
4561 gattctatta acaagggtat caccttcaaa cttgacttca gcacgtgtct tgtagttccc
4621 gtcatctttg aaaaatatag ttctttcctg tacataacct tcgggcatgg cactcttgaa
4681 aaagtcatgc tgtttcatat gatctgggta tctcgcaaag cattgaacac cataaccgaa
4741 agtagtgaca agtgttggcc atggaacagg tagttttcca gtagtgcaaa taaatttaag
4801 ggtaagtttt ccgtatgttg catcaccttc accctctcca ctgacagaaa atttgtgccc
4861 attaacatca ccatctaatt caacaagaat tgggacaact ccagtgaaaa gttcttctcc
4921 tttactcata tgtatatctc cttcttaaat ctagatttaa agatctggta ccgggccccc
4981 cctcgagggg gggcccgttt ctcaaagctc acgctgtagg tatctcagtt cggtgtaggt
5041 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
5101 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
5161 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
5221 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
5281 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
5341 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
5401 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
5461 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
5521 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
5581 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
5641 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
5701 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
5761 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
5821 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
5881 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
5941 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
6001 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
6061 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
6121 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
6181 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
6241 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
6301 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
6361 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
6421 aatactcata ctcttccttt ttcaatcagg tggcactttt cggggaaatg tgcgcgcccg
6481 cgttcctgct ggcgctgggc ctgtttctgg cgctggactt cccgctgttc cgtcagcagc
6541 ttttcgccca cggccttgat gatcgcggcg gccttggcct gcatatcccg attcaacggc
6601 cccagggcgt ccagaacggg cttcaggcgc tcccgaaggt
//
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