pAC-ZEAXipi

LOCUS       pAC-ZEAXipi               10041 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Contains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia 
            herbicola (Pantoea agglomerans) Eho10 and thereby produces 
            zeaxanthin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    pAC-ZEAXipi
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10041)
  AUTHORS   Cunningham FX Jr, Gantt E
  TITLE     A portfolio of plasmids for identification and analysis of 
            carotenoid pathway enzymes: Adonis aestivalis as a case study.
  JOURNAL   Photosynth Res. 2007 May;92(2):245-59. Epub 2007 Jul 17.
  PUBMED    17634749
REFERENCE   2  (bases 1 to 10041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10041
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     134..153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(714..731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(928..947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1505..1533
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             complement(9601..219)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
     1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
     1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
     1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
     1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
     1681 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt
     1741 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc
     1801 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac
     1861 ccgtcctgtg gatctaaagg cacagcgtct catgcttcgc acaatgtaaa actgcttcag
     1921 aacctggcga gagctatccg cgcggtctac ggttaactga tactaaaaga caattcagcg
     1981 ggtaaccttg caatggtgag tggcagtaaa gcgggcgttt cgcctcatcg cgaaatagaa
     2041 gtaatgagac aatccattga cgatcacctg gctggcctgt tacctgaaac cgacagccag
     2101 gatatcgtca gccttgcgat gcgtgaaggc gtcatggcac ccggtaaacg gatccgtccg
     2161 ctgctgatgc tgctggccgc ccgcgacctc cgctaccagg gcagtatgcc tacgctgctc
     2221 gatctcgcct gcgccgttga actgacccat accgcgtcgc tgatgctcga cgacatgccc
     2281 tgcatggaca acgccgagct gcgccgcggt cagcccacta cccacaaaaa atttggtgag
     2341 agcgtggcga tccttgcctc cgttgggctg ctctctaaag cctttggtct gatcgccgcc
     2401 accggcgatc tgccggggga gaggcgtgcc caggcggtca acgagctctc taccgccgtg
     2461 ggcgtgcagg gcctggtact ggggcagttt cgcgatctta acgatgccgc cctcgaccgt
     2521 acccctgacg ctatcctcag caccaaccac ctcaagaccg gcattctgtt cagcgcgatg
     2581 ctgcagatcg tcgccattgc ttccgcctcg tcgccgagca cgcgagagac gctgcacgcc
     2641 ttcgccctcg acttcggcca ggcgtttcaa ctgctggacg atctgcgtga cgatcacccg
     2701 gaaaccggta aagatcgcaa taaggacgcg ggaaaatcga cgctggtcaa ccggctgggc
     2761 gcagacgcgg cccggcaaaa gctgcgcgag catattgatt ccgccgacaa acacctcact
     2821 tttgcctgtc cgcagggcgg cgccatccga cagtttatgc atctgtggtt tggccatcac
     2881 cttgccgact ggtcaccggt catgaaaatc gcctgatacc gcccttttgg gttcaagcag
     2941 tacataacga tggaaccaca ttacaggagt agtgatgaat gaaggacgag cgccttgttc
     3001 agcgtaagaa cgatcatctg gatatcgttc tcgacccccg tcgcgccgta actcaggcta
     3061 gcgcaggttt tgagcgctgg cgctttaccc actgcgccct gccagagctg aattttagcg
     3121 acatcacgct ggaaaccacc ttcctgaatc ggcagctaca ggctccgctg ctgatcagct
     3181 ccatgaccgg cggcgttgag cgctcgcgcc atatcaaccg ccacctcgcc gaggcggcgc
     3241 aggtgctaaa aattgcgatg ggggtgggct cccagcgcgt cgccattgag agcgacgcgg
     3301 gcttagggct ggataaaacc ctgcggcagc tggctccgga cgtgccgctg ctggcgaacc
     3361 tcggcgcggc gcagctgacc ggcagaaaag gtattgatta cgcccgacgg gccgtggaga
     3421 tgatcgaggc ggatgcgctg attgtgcacc taaacccgct gcaggaggcg ctacagcccg
     3481 gcggcgatcg cgactggcgc ggacggctgg cggctattga aactctggtc cgcgagctgc
     3541 ccgttccgct ggtggtgaaa gaggtgggag ccggtatctc ccgaaccgtg gccgggcagc
     3601 tgatcgatgc cggcgttacc gtgattgacg tcgcgggcgc gggcggcacc agctgggccg
     3661 ccgttgaagg cgagcgggcg gccaccgagc agcagcgcag cgtggccaac gtctttgccg
     3721 actgggggat ccccaccgct gaggcgctgg ttgacattgc cgaggcctgg ccgcagatgc
     3781 cccttattgc ctcgggcggg attaaaaacg gcgtcgacgc ggcgaaagcg ctgcggctcg
     3841 gcgcgtgcat ggtagggcag gccgccgccg tgctcggcag cgcaggcgtc tccacggaga
     3901 aggtgatcga tcacttcaac gtgattattg agcagctgcg ggtggcctgc ttctgcaccg
     3961 gcagccgcag cctgagcgat ctaaagcagg ctgatatccg ctatgtgcgg gatacgccat
     4021 gagccatttt gccattgtgg caccgccgct ctacagtcat gcggtggcgc tgcatgccct
     4081 gtgggagcac tgcgctatca gccgccgccg caggtagaac gctcgccacg cagcacgccg
     4141 cggatctttg cctcgctggg caccctccag ggccaccgtc tacgcctgtt tcagaagatc
     4201 gcccgcgcct gtgccagcgt gggggcggag gtgaccattg cccactgcga tggcctgacg
     4261 cccgcccagg ccgactcgct ctacgcctgc ggcgcgacgg aggtggtcag ctttgtcgac
     4321 cagccgcgct acgttgccga ggctaatctg gtgatcaccc acggcggtct caataccgta
     4381 ctggatgcgc tggctgccgc gacgccggtg ctggcggtgc cactctcttt cgaccagccc
     4441 gccgtggctg cccggctggt ctataacggg ctgggtcgcc gggtatcgcg ctttgccaga
     4501 cagcagacgc tggcggatga gattgcccaa ctgctggggg atgagacgct gcatcagcgt
     4561 ctggcgacgg cccgccagca gcttaacgac gccgggggca cgccccgtgc ggcgaccctg
     4621 attgaacagg ccatagcagg gagtgagagc gtatcgtgag ggatctgatt ttagtcggcg
     4681 gcggcctggc caacgggctg atcgcctggc gtctgcgcca gcgctacccg cagcttaacc
     4741 tgctgctgat cgaggccggg gagcagcccg gcgggaacca tacctggtca ttccatgaag
     4801 acgatctgac tcccgggcag cacgcctggc tggccccgct ggtggcccac gcctggccgg
     4861 gctatgaggt gcagtttccc gatcttcgcc gtcgcctcgc gcgcggctac tactccatta
     4921 cctcagagcg ctttgccgag gccctgcatc aggcgctggg ggagaacatc tggctaaact
     4981 gttcggtgag cgaggtgtta cccaatagcg tgcgccttgc caacggtgag gcgctgcttg
     5041 ccggagcggt gattgacgga cgcggcgtga ccgccagttc ggcgatgcaa accggctatc
     5101 agctctttct tggtcagcag tggcggctga cacagcccca cggcctgacc gtaccgatcc
     5161 tgatggatgc cacggtggcg cagcagcagg gctatcgctt tgtctacacg ctgccgctct
     5221 ccgccgacac gctgctgatc gaggatacgc gctacgccaa tgtcccgcag cgtgatgata
     5281 atgccctacg ccagacggtt accgactatg ctcacagcaa agggtggcag ctggcccagc
     5341 ttgaacgcga ggagaccggc tgtctgccga ttaccctggc gggtgacatc caggctctgt
     5401 gggccgatgc gccgggcgtg ccgcgctcgg gaatgcgggc tgggctattt caccctacca
     5461 ctggctattc gctgccgctg gcggtggccc ttgccgacgc gattgccgac agcccgcggc
     5521 tgggcagcgt tccgctctat cagctcaccc ggcagtttgc cgaacgccac tggcgcaggc
     5581 agggattctt ccgcctgctg aaccggatgc ttttcctggc cgggcgcgag gagaaccgct
     5641 ggcgggtgat gcagcgcttt tatgggctgc cggagcccac cgtagagcgc ttttacgccg
     5701 gtcggctctc tctctttgat aaggcccgca ttttgacggg caagccaccg gttccgctgg
     5761 gcgaagcctg gcgggcggcg ctgaaccatt ttcctgacag acgagataaa ggatgaaaaa
     5821 aaccgttgtg attggcgcag gctttggtgg cctggcgctg gcgattcgcc tgcaggcggc
     5881 agggatccca accgtactgc tggagcagcg ggacaagccc ggcggtcggg cctacgtctg
     5941 gcatgaccag ggctttacct ttgacgccgg gccgacggtg atcaccgatc ctaccgcgct
     6001 tgaggcgctg ttcaccctgg ccggcaggcg catggaggat tacgtcaggc tgctgccggt
     6061 aaaacccttc taccgactct gctgggagtc cgggaagacc ctcgactatg ctaacgacag
     6121 cgccgagctt gaggcgcaga ttacccagtt caacccccgc gacgtcgagg gctaccggcg
     6181 ctttctggct tactcccagg cggtattcca ggagggatat ttgcgcctcg gcagcgtgcc
     6241 gttcctctct tttcgcgaca tgctgcgcgc cgggccgcag ctgcttaagc tccaggcgtg
     6301 gcagagcgtc taccagtcgg tttcgcgctt tattgaggat gagcatctgc ggcaggcctt
     6361 ctcgttccac tccctgctgg taggcggcaa ccccttcacc acctcgtcca tctacaccct
     6421 gatccacgcc cttgagcggg agtggggggt ctggttccct gagggcggca ccggggcgct
     6481 ggtgaacggc atggtgaagc tgtttaccga tctgggcggg gagatcgaac tcaacgcccg
     6541 ggtcgaagag ctggtggtgg ccgataaccg cgtaagccag gtccggctgg cggatggtcg
     6601 gatctttgac accgacgccg tagcctcgaa cgctgacgtg gtgaacacct ataaaaagct
     6661 gctcggccac catccggtgg ggcagaagcg ggcggcagcg ctggagcgca agagcatgag
     6721 caactcgctg tttgtgctct acttcggcct gaaccagcct cattcccagc tggcgcacca
     6781 taccatctgt tttggtcccc gctaccggga gctgatcgac gagatcttta ccggcagcgc
     6841 gctggcggat gacttctcgc tctacctgca ctcgccctgc gtgaccgatc cctcgctcgc
     6901 gcctcccggc tgcgccagct tctacgtgct ggccccggtg ccgcatcttg gcaacgcgcc
     6961 gctggactgg gcgcaggagg ggccgaagct gcgcgaccgc atctttgact accttgaaga
     7021 gcgctatatg cccggcctgc gtagccagct ggtgacccag cggatcttta ccccggcaga
     7081 cttccacgac acgctggatg cgcatctggg atcggccttc tccatcgagc cgctgctgac
     7141 ccaaagcgcc tggttccgcc cgcacaaccg cgacagcgac attgccaacc tctacctggt
     7201 gggcgcaggt actcaccctg gggcgggcat tcctggcgta gtggcctcgg cgaaagccac
     7261 cgccagcctg atgattgagg atctgcaatg agccaaccgc cgctgcttga ccacgccacg
     7321 cagaccatgg ccaacggctc gaaaagtttt gccaccgctg cgaagctgtt cgacccggcc
     7381 acccgccgta gcgtgctgat gctctacacc tggtgccgcc actgcgatga cgtcattgac
     7441 gaccagaccc acggcttcgc cagcgaggcc gcggcggagg aggaggccac ccagcgcctg
     7501 gcccggctgc gcacgctgac cctggcggcg tttgaagggg ccgagatgca ggatccggcc
     7561 ttcgctgcct ttcaggaggt ggcgctgacc cacggtatta cgccccgcat ggcgctcgat
     7621 cacctcgacg gctttgcgat ggacgtggct cagacccgct atgtcacctt tgaggatacg
     7681 ctgcgctact gctatcacgt ggcgggcgtg gtgggtctga tgatggccag ggtgatgggc
     7741 gtgcgggatg agcgggtgct ggatcgcgcc tgcgatctgg ggctggcctt ccagctgacg
     7801 aatatcgccc gggatattat tgacgatgcg gctattgacc gctgctatct gcccgccgag
     7861 tggctgcagg atgccgggct gaccccggag aactatgccg cgcgggagaa tcgggccgcg
     7921 ctggcgcggg tggcggagcg gcttattgat gccgcagagc cgtactacat ctcctcccag
     7981 gccgggctac acgatctgcc gccgcgctgc gcctgggcga tcgccaccgc ccgcagcgtc
     8041 taccgggaga tcggtattaa ggtaaaagcg gcgggaggca gcgcctggga tcgccgccag
     8101 cacaccagca aaggtgaaaa aattgccatg ctgatggcgg caccggggca ggttattcgg
     8161 gcgaagacga cgagggtgac gccgcgtccg gccggtcttt ggcagcgtcc cgtttaggcg
     8221 ggcggccatg acgttcacgc aggatcgcct gtaggtcggc aggcttgcgg gcgtaaataa
     8281 aaccgaagga gacgcagccc tcccggccgc gcaccgcgtg gtgcaggcgg tgggcgacgt
     8341 agagccgctt caggtagccc cggcgcggga tccagtggaa gggccagcgc tgatgcacca
     8401 gaccgtcgtg caccaggaag tagagcaggc catagaccgt catgccgcag ccaatccact
     8461 gcaggggcca aacgcccgcc gtgcccacgg caatcagcgc gatagccacc ccggcaaaca
     8521 ccaccgcaaa gagatcgttt agctcaaata cgcccttgcg cggggtatgg tgtgactcat
     8581 gccagcgcca tccccagccg tgcataatgt agcggtgggt aaacgcggcg atgccctcca
     8641 tcgcaataac gctcaagatg acgattaaac tatttactag catagcggtg cgcgctcctg
     8701 cgactcagac tggcttaatc aaaaccctta gcctctaagc atagtcgcaa tcgcaggcgg
     8761 cgcgcatcgg tatcaggcca gagcctgctg cagatccgcc agcagatcct tctcatcctc
     8821 aatgcccacc gacaggcgga tcagctgcgg cgtaatgccg ttcgccaggc gctgcgccag
     8881 cgggatcgag gcgtgggtca tactgaacgg ctggctcacc aggctctcaa cgccgcccag
     8941 gctctcagcg agggtaaaca gcttcagttt actgatcacc tctgccgcac gctggtcgtc
     9001 gcctttgatc acgatggaga tcatgccgcc cggctgagcc atctgctggc gcgcgagggc
     9061 atactgcggg tgggatgtca gccacgggaa ccagactttc tcgacctgcg gctgctgctc
     9121 aagccagcgg gccagcgcca gggcgttgct gctgtggcgc tcgatgcgca gggcgagggt
     9181 gcggatcccg cgcagggtca ggaagctgct gaacggatcg agcacgccgc ccaccgcatt
     9241 ttgcaggtag cccagcttct ccgccagcgc cggacgatcg cccaccaccg ccagcccggc
     9301 gaccacgtcg gagtggccgt tgaggtattt ggtggccgag tgcaccacga tatcaaagcc
     9361 cagcgtcagc gggcggtgga tcagcggcga ggcaaaggta ttatctgcca cgctaatcac
     9421 gttatgacgg cgggcgatct cagcgatagc cgccaggtcc gccagcttca acagcgggtt
     9481 ggtgggggtc tctacccggg tcgaatttgc tttcgaattt ctgccattca tccgcttatt
     9541 atcacttatt caggcgtagc aaccaggcgt ttaagggcac caataactgc cttaaaaaaa
     9601 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
     9661 atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     9721 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
     9781 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
     9841 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     9901 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     9961 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
    10021 accgtctttc attgccatac g
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