| 质粒名称: | Clover-Geminin(1-110) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 83915 |
| 存储实验室: | Michael Lin |
|
载体骨架基本信息 |
|
| 载体名称: | pLL3.7m |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6500 bp |
| 完整质粒大小: | 7475 bp |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Clover-Geminin(1-110) |
| 物种来源: | Synthetic |
| 插入大小: | 1080 bp |
| 启动子 | CMV |
| 5' 测序引物 | agctggtttagtgaaccgtcagatc |
| 3' 测序引物 | ggaaccggaacccttaaaca |
|
文献引用方法 |
|
| 材料和方法部分: | Clover-Geminin(1-110) was a gift from Michael Lin (Addgene plasmid # 83915 ; http://n2t.net/addgene:83915 ; RRID:Addgene_83915) |
| 参考文献部分: | Fluorescent indicators for simultaneous reporting of all four cell cycle phases. Bajar BT, Lam AJ, Badiee RK, Oh YH, Chu J, Zhou XX, Kim N, Kim BB, Chung M, Yablonovitch AL, Cruz BF, Kulalert K, Tao JJ, Meyer T, Su XD, Lin MZ. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4045. 10.1038/nmeth.4045 PubMed 27798610 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS Clover-Geminin(1-110) 7475 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Fluorescent probe for M/G1 transition.
ACCESSION .
VERSION .
KEYWORDS Clover-Geminin(1-110)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7475)
AUTHORS Bajar BT, Lam AJ, Badiee RK, Oh YH, Chu J, Zhou XX, Kim N, Kim BB,
Chung M, Yablonovitch AL, Cruz BF, Kulalert K, Tao JJ, Meyer T, Su
XD, Lin MZ
TITLE Fluorescent indicators for simultaneous reporting of all four cell
cycle phases.
JOURNAL Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4045.
PUBMED 27798610
REFERENCE 2 (bases 1 to 7475)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7475
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 63..366
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 368..566
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 584..764
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 811..936
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1435..1668
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2195..2312
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2371..2684
/label=U6 promoter
/note="RNA polymerase III promoter for mouse U6 snRNA (Das
et al., 1988)"
primer_bind 2603..2623
/label=mU6-F
/note="Mouse U6 promoter, forward primer"
primer_bind 2701..2717
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 2759..2792
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
enhancer 2908..3211
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3212..3415
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 3447..4163
/codon_start=1
/product="bright green-yellow fluorescent protein derived
from GFP (Lam et al., 2012)"
/label=Clover
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
EFVTAAGITHGMDELYK"
primer_bind complement(3492..3513)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind 4100..4121
/label=EGFP-C
/note="EGFP, forward primer"
protein_bind 4562..4595
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 4651..5239
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4704..4724)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5122..5133)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(5242..5258)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
LTR 5481..5661
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
rep_origin complement(5723..6311)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5803..5822)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6482..7342)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7105..7124
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7343..7447)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
541 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
601 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
661 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
721 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
781 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
841 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
901 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
961 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
1021 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
1081 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
1141 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
1201 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
1261 accgcacagc aagcggccgg ccgcgctgat cttcagacct ggaggaggag atatgaggga
1321 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
1381 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
1441 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
1501 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
1561 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
1621 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
1681 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
1741 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
1801 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
1861 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
1921 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
1981 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
2041 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
2101 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
2161 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
2221 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
2281 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
2341 cagtttggtt agtaccgggc ccgctctaga gatccgacgc cgccatctct aggcccgcgc
2401 cggccccctc gcacagactt gtgggagaag ctcggctact cccctgcccc ggttaatttg
2461 catataatat ttcctagtaa ctatagaggc ttaatgtgcg ataaaagaca gataatctgt
2521 tctttttaat actagctaca ttttacatga taggcttgga tttctataag agatacaaat
2581 actaaattat tattttaaaa aacagcacaa aaggaaactc accctaactg taaagtaatt
2641 gtgtgttttg agactataaa tatcccttgg agaaaagcct tgttaacgcg cggtgaccct
2701 cgaggtcgac ggtatcgata agctcgcttc acgagattcc agcaggtcga gggacctaat
2761 aacttcgtat agcatacatt atacgaagtt atattaaggg ttccaagctt aagcggccgc
2821 gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt
2881 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
2941 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
3001 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
3061 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
3121 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
3181 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
3241 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
3301 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
3361 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
3421 agtgaaccgt cagatccgct agcaccatgg tgagcaaggg cgaggagctg ttcaccgggg
3481 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtccgcg
3541 gcgagggcga gggcgatgcc accaacggca agctgaccct gaagttcatc tgcaccaccg
3601 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc gtggcctgct
3661 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
3721 gctacgtcca ggagcgcacc atctctttca aggacgacgg tacctacaag acccgcgccg
3781 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
3841 aggaggacgg caacatcctg gggcacaagc tggagtacaa cttcaacagc cacaacgtct
3901 atatcacggc cgacaagcag aagaacggca tcaaggctaa cttcaagatc cgccacaacg
3961 ttgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
4021 gccccgtgct gctgcccgac aaccactacc tgagccatca gtccgccctg agcaaagacc
4081 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggattacac
4141 atggcatgga cgagctgtac aaggatatcc atgcacactg gcggccgctc gagatgaatc
4201 ccagtatgaa gcagaaacaa gaagaaatca aagagaatat aaagaatagt tctgtcccaa
4261 gaagaactct gaagatgatt cagccttctg catctggatc tcttgttgga agagaaaatg
4321 agctgtccgc aggcttgtcc aaaaggaaac atcggaatga ccacttaaca tctacaactt
4381 ccagccctgg ggttattgtc ccagaatcta gtgaaaataa aaatcttgga ggagtcaccc
4441 aggagtcatt tgatcttatg attaaagaaa atccatcctc tcagtattgg aaggaagtgg
4501 cagaaaaacg gagaaaggcg ctgtaagttt aaaccctgca gggaattcgt cgagggacct
4561 aataacttcg tatagcatac attatacgaa gttatacatg tttaagggtt ccggttccac
4621 taggtacaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
4681 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
4741 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
4801 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
4861 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
4921 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
4981 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
5041 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
5101 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
5161 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
5221 ctttgggccg cctccccgca tcgataccgt cgacctcgat cgagacctag aaaaacatgg
5281 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
5341 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
5401 caaggcagct gacgcgtgat tttaaaagaa aaggggggac tggaagggct aattcactcc
5461 cactgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
5521 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
5581 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
5641 cagtgtggaa aatctctagc agcatgtgag caaaaggcca gcaaaaggcc aggaaccgta
5701 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
5761 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
5821 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
5881 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
5941 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
6001 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
6061 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
6121 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
6181 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
6241 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
6301 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
6361 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
6421 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
6481 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
6541 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
6601 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
6661 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
6721 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
6781 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
6841 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
6901 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
6961 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
7021 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
7081 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
7141 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
7201 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
7261 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
7321 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
7381 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
7441 ttccgcgcac atttccccga aaagtgccac ctgac
//
