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pLOX-Ttag-iresTK载体质粒基本信息
pLOX-Ttag-iresTK质粒图谱载体图谱和pLOX-Ttag-iresTK载体序列质粒序列多克隆位点信息
pLOX-Ttag-iresTK质粒载体简介
pLOX-Ttag-iresTK质粒序列载体序列
LOCUS pLOX-Ttag-iresTK 12290 bp DNA SYN DEFINITION pLOX-Ttag-iresTK ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..12290 /organism="pLOX-Ttag-iresTK" /mol_type="other DNA" misc_feature 457..637 /label="truncHIV-1_3_LTR" misc_feature 457..637 /label="HIV-1_5_LTR" misc_feature 748..792 /label="HIV-1_psi_pack" CDS 1180..2028 /label="ORF frame 1" misc_feature 1302..1535 /label="RRE" promoter 2040..2544 /label="CMV_immearly_promoter" misc_feature 2047..2334 /label="CAG_enhancer" misc_feature 2501..2521 /label="CMV_fwd_primer" promoter 2502..2571 /label="CMV_promoter" misc_feature 2545..2564 /label="pCEP_fwd_primer" misc_feature 2547..2571 /label="LNCX_primer" CDS 2731..3255 /label="ORF frame 1" misc_feature 3256..3271 /label="SV40_int" misc_feature 3277..3324 /label="SV40_3_splice" misc_feature 3335..5203 /label="SV40_lrgT" CDS 3401..5203 /label="ORF frame 2" misc_feature 3452..3472 /label="SV40 NLS" misc_feature 3458..3472 /label="NLS" misc_feature complement(5257..5273) /label="pBluescriptKS_primer" misc_feature 5293..5783 /label="IRES" CDS 5787..6701 /label="ORF frame 3" misc_feature 6782..6803 /label="U3PPT" misc_feature 6782..6797 /label="cPPT" misc_feature 6854..6887 /label="loxP" misc_feature 6907..7087 /label="truncHIV-1_3_LTR" misc_feature 6907..7087 /label="HIV-1_5_LTR" misc_feature complement(7113..7130) /label="Sp6_primer" gene 7368..7631 /label="tet (300 - 563)" /gene="tet (300 - 563)" misc_feature complement(7420..7439) /label="pBRrevBam_primer" misc_feature 7772..7794 /label="pGEX_3_primer" promoter 7953..7981 /label="AmpR_promoter" gene 8023..8883 /label="Ampicillin" /gene="Ampicillin" CDS 8023..8883 /label="ORF frame 1" rep_origin 9038..9657 /label="pBR322_origin" misc_feature complement(9883..9903) /label="pBABE_3_primer" misc_feature complement(9889..10342) /label="SV40_enhancer" promoter 9901..10169 /label="SV40_promoter" rep_origin 10068..10145 /label="SV40_origin" misc_feature 10130..10149 /label="SV40pro_F_primer" misc_feature 11350..11365 /label="SV40_int" misc_feature 11371..11418 /label="SV40_3_splice" misc_feature 11429..5203 /label="SV40_lrgT" CDS 11495..11968 /label="ORF frame 2" misc_feature 11546..11566 /label="SV40 NLS" misc_feature 11552..11566 /label="NLS" terminator 11994..12125 /label="SV40_PA_terminator" misc_feature 12082..12101 /label="EBV_rev_primer" ORIGIN 1 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc 61 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc 121 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg 181 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc 241 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc 301 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt 361 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca 421 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc 481 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 541 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 601 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga 661 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca 721 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct 781 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat 841 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat 901 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag 961 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta 1021 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag 1081 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac 1141 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt 1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca 1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg 1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc 1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg 1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg 1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa 1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag 1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta 1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg 1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat 1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta 1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc 1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac 1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgataagc ttgggagttc 2041 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca 2101 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt 2161 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg 2221 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag 2281 tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt 2341 accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg 2401 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa 2461 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt 2521 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga 2581 cgccatccac gctgttttga cctccataga agacaccgac tctagctaga ggatcccccg 2641 ggctgcagga attcccggcc tctgagctat tccagaagta gtgaagaggc ttttttggag 2701 gcctaggctt ttgcaaaaag ctttgcaaag atggataaag ttttaaacag agaggaatct 2761 ttgcagctaa tggaccttct aggtcttgaa aggagtgcct gggggaatat tcctctgatg 2821 agaaaggcat atttaaaaaa atgcaaggag tttcatcctg ataaaggagg agatgaagaa 2881 aaaatgaaga aaatgaatac tctgtacaag aaaatggaag atggagtaaa atatgctcat 2941 caacctgact ttggaggctt ctgggatgca actgaggtat ttgcttcttc cttaaatcct 3001 ggtgttgatg caatgtactg caaacaatgg cctgagtgtg caaagaaaat gtctgctaac 3061 tgcatatgct tgctgtgctt actgaggatg aagcatgaaa atagaaaatt atacaggaaa 3121 gatccacttg tgtgggttga ttgctactgc ttcgattgct ttagaatgtg gtttggactt 3181 gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga 3241 gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat 3301 tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg 3361 gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga 3421 ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc 3481 caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac 3541 tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat 3601 tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc ataacatact 3661 gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt 3721 gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc 3781 cttgactaga gatccatttt ctgttattga ggaaagtttg ccaggtgggt taaaggagca 3841 tgattttaat ccagaagaag cagaggaaac taaacaagtg tcctggaagc ttgtaacaga 3901 gtatgcaatg gaaacaaaat gtgatgatgt gttgttattg cttgggatgt acttggaatt 3961 tcagtacagt tttgaaatgt gtttaaaatg tattaaaaaa gaacagccca gccactataa 4021 gtaccatgaa aagcattatg caaatgctgc tatatttgct gacagcaaaa accaaaaaac 4081 catatgccaa caggctgttg atactgtttt agctaaaaag cgggttgata gcctacaatt 4141 aactagagaa caaatgttaa caaacagatt taatgatctt ttggatagga tggatataat 4201 gtttggttct acaggctctg ctgacataga agaatggatg gctggagttg cttggctaca 4261 ctgtttgttg cccaaaatgg attcagtggt gtatgacttt ttaaaatgca tggtgtacaa 4321 cattcctaaa aaaagatact ggctgtttaa aggaccaatt gatagtggta aaactacatt 4381 agcagctgct ttgcttgaat tatgtggggg gaaagcttta aatgttaatt tgcccttgga 4441 caggctgaac tttgagctag gagtagctat tgaccagttt ttagtagttt ttgaggatgt 4501 aaagggcact ggaggggagt ccagagattt gccttcaggt cagggaatta ataacctgga 4561 caatttaagg gattatttgg atggcagtgt taaggtaaac ttagaaaaga aacacctaaa 4621 taaaagaact caaatatttc cccctggaat agtcaccatg aatgagtaca gtgtgcctaa 4681 aacactgcag gccagatttg taaaacaaat agattttagg cccaaagatt atttaaagca 4741 ttgcctggaa cgcagtgagt ttttgttaga aaagagaata attcaaagtg gcattgcttt 4801 gcttcttatg ttaatttggt acagacctgt ggctgagttt gctcaaagta ttcagagcag 4861 aattgtggag tggaaagaga gattggacaa agagtttagt ttgtcagtgt atcaaaaaat 4921 gaagtttaat gtggctatgg gaattggagt tttagattgg ctaagaaaca gtgatgatga 4981 tgatgaagac agccaggaaa atgctgataa aaatgaagat ggtggggaga agaacatgga 5041 agactcaggg catgaaacag gcattgattc acagtcccaa ggctcatttc aggcccctca 5101 gtcctcacag tctgttcatg atcataatca gccataccac atttgtagag gttttacttg 5161 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg 5221 ttgttagcgg ccccggaatt cgatatcaag cttatcgata ccgtcgacct cgacattaat 5281 tccggttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc 5341 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc 5401 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg 5461 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa 5521 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt 5581 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg 5641 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta 5701 catgtgttta gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt 5761 cctttgaaaa acacgatgat aataccatgg cttcgtaccc ctgccatcaa cacgcgtctg 5821 cgttcgacca ggctgcgcgt tctcgcggcc ataacaaccg acgtacggcg ttgcgccctc 5881 gccggcaaca aaaagccacg gaagtccgcc tggagcagaa aatgcccacg ctactgcggg 5941 tttatataga cggtccccac gggatgggga aaaccaccac cacgcaactg ctggtggccc 6001 tgggttcgcg cgacgatatc gtctacgtac ccgagccgat gacttactgg cgggtgttgg 6061 gggcttccga gacaatcgcg aacatctaca ccacacaaca ccgcctcgac cagggtgaga 6121 tatcggccgg ggacgcggcg gtggtaatga caagcgccca gataacaatg ggcatgcctt 6181 atgccgtgac cgacgccgtt ctggctcctc atatcggggg ggaggctggg agctcacatg 6241 ccccgccccc ggccctcacc ctcatcttcg accgccatcc catcgccgcc ctcctgtgct 6301 acccggccgc gcgatacctt atgggcagca tgacccccca ggccgtgctg gcgttcgtgg 6361 ccctcatccc gccgaccttg cccggcacaa acatcgtgtt gggggccctt ccggaggaca 6421 gacacatcga ccgcctggcc aaacgccagc gccccggcga gcggcttgac ctggctatgc 6481 tggccgcgtc gccgcgttta tgggctgctt gccaatacgg tgcggtatct gcagggcggc 6541 gggtcgtggc gggaggattg gggacagctt tcgggggcgg ccgtgccgcc ccagggtgcc 6601 gagccccaga gcaacgcggg cccacgaccc catatcgggg acacgttatt taccctgttt 6661 cgggcccccg agttgctggc ccccaacggc gacctgtata acgtgtttgc ctgggctttg 6721 gctcgacggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt 6781 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatgggatca 6841 attcaccatg ggaataactt cgtatagcat acattatacg aagttatgct gctttttgct 6901 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 6961 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 7021 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 7081 tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat cgtatgtgta 7141 tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta 7201 attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca 7261 gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa 7321 tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg 7381 tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg 7441 atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg 7501 tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg 7561 cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc 7621 ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta 7681 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg 7741 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca 7801 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt 7861 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc 7921 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 7981 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 8041 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 8101 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 8161 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 8221 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 8281 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 8341 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 8401 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 8461 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 8521 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 8581 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 8641 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 8701 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 8761 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 8821 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 8881 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 8941 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 9001 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 9061 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 9121 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 9181 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 9241 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 9301 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 9361 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 9421 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag 9481 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 9541 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 9601 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 9661 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 9721 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 9781 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 9841 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag 9901 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 9961 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 10021 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 10081 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 10141 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 10201 gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata 10261 ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata 10321 ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact 10381 ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc 10441 tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa 10501 tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg 10561 aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc 10621 gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg 10681 acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact 10741 ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata 10801 tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa 10861 tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca 10921 ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac 10981 ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc 11041 cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct 11101 cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa 11161 atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta 11221 tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt 11281 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta 11341 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat 11401 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc 11461 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac 11521 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga 11581 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc 11641 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga 11701 aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt 11761 tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac 11821 ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac 11881 tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc 11941 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta 12001 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 12061 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 12121 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta 12181 ttaccactgc caattaccta gtggtttcat ttactctaaa cctgtgattc ctctgaatta 12241 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt //
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pLOX-Ttag-iresTK质粒载体应用举例
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