| 出品公司: | Clontech |
|---|---|
| 载体名称: | pIRES |
| 质粒类型: | 哺乳动物细胞表达载体；双顺反子载体；双表达载体 |
| 启动子: | CMV |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6092 bp |
| 5' 测序引物: | CMV-F: 5'-CGCAAATGGGCGGTAGGCGTG-3' |
| 3' 测序引物: | pIRES-R:5'-GCCCTAGATGCATGCTCG-3' |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素（Ampicillin） |
| 筛选标记: | 新霉素（Neomycin） |
| 备注: |
pIRES载体含有IRES元件，可以同时表达两个基因。
|
| 产品目录号: | 631605 |
| 稳定性: | 稳表达 或 瞬表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pIRES载体质粒基本信息
pIRES质粒图谱载体图谱和pIRES载体序列质粒序列多克隆位点信息
pIRES质粒载体简介
pIRES is a mammalian expression vector that allows high level expression of two genes of interest from the same bicistronic mRNA transcript. The vector contains the encephalomyocarditis virus (ECMV) internal ribosome entry site (IRES) flanked by two multiple cloning sites (MCS A and B), an arrangement that allows cap-independent translation of the gene cloned into MCS B (1–3). pIRES utilizes a partially disabled IRES sequence (1) that reduces the rate at which the gene cloned into MCS B is translated relative to that of MCS A.
Expression of the bicistronic transcript is driven by the constitutively active cytomegalovirus immediate early promoter (PCMV IE), located upstream of MCS A. An intervening sequence (IVS) known to enhance the stability of mRNA (4) is located between PCMV IE and MCS A, and is efficiently spliced out following transcription. SV40 polyadenylation signals downstream of MCS B direct proper processing of the 3' end of the mRNA. Bacteriophage T7 and T3 promoters are located upstream of MCS A and downstream of MCS B, respectively. pIRES includes a neomycin resistance gene (Neor) to aid in the selection of transfected cells. Neor is expressed from the SV40 enhancer/promoter, and a synthetic polyadenyla¬tion signal directs proper processing of the 3' end of the Neor mRNA. The SV40 origin al¬lows for replication in mammalian cells expressing the SV40 T antigen. The vector also contains an ampicillin resistance gene (Ampr), and a ColE1 origin of replication for se¬lection and propagation in E. coli, and an f1 origin for single-stranded DNA production.
pIRES质粒序列载体序列
LOCUS pIRES 6092 bp DNA SYN DEFINITION pIRES ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT ORIGDB|GenBank COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers source 1..6092 /organism="pIRES" /mol_type="other DNA" promoter 163..723 /label="CMV_immearly_promoter" misc_feature 218..505 /label="CAG_enhancer" promoter 669..750 /label="CMV_promoter" misc_feature 680..700 /label="CMV_fwd_primer" misc_feature 724..743 /label="pCEP_fwd_primer" misc_feature 726..750 /label="LNCX_primer" misc_feature 890..1022 /label="chim_int" promoter 1067..1085 /label="T7_promoter" misc_feature 1136..1699 /label="IRES" terminator complement(1791..1910) /label="SV40_PA_terminator" misc_feature complement(1805..1824) /label="EBV_rev_primer" rep_origin 2118..2424 /label="f1_origin" misc_feature complement(2698..2718) /label="pBABE_3_primer" misc_feature complement(2704..2919) /label="SV40_enhancer" promoter 2716..2984 /label="SV40_promoter" rep_origin 2883..2960 /label="SV40_origin" misc_feature 2945..2964 /label="SV40pro_F_primer" CDS 3083..3877 /label="ORF frame 2" gene 3086..3874 /label="NeoR/KanR" /gene="NeoR/KanR" CDS complement(3392..3928) /label="ORF frame 2" terminator 3941..3989 /label="synth_PA_terminator" misc_feature 4149..4171 /label="pGEX_3_primer" promoter 4330..4358 /label="AmpR_promoter" gene 4400..5260 /label="Ampicillin" /gene="Ampicillin" CDS 4400..5260 /label="ORF frame 2" rep_origin 5415..6034 /label="pBR322_origin" ORIGIN 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA ATATTGGCTA 61 TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC ATTTATATTG GCTCATGTCC 121 AATATGACCG CCATGTTGGC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG 181 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC 241 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC 361 CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG CCCCCTATTG ACGTCAATGA 421 CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTACGGGACT TTCCTACTTG 481 GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC 541 CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTG 661 CGATCGCCCG CCCCGTTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 721 AGCAGAGCTC GTTTAGTGAA CCGTCAGATC ACTAGAAGCT TTATTGCGGT AGTTTATCAC 781 AGTTAAATTG CTAACGCAGT CAGTGCTTCT GACACAACAG TCTCGAACTT AAGCTGCAGT 841 GACTCTCTTA AGGTAGCCTT GCAGAAGTTG GTCGTGAGGC ACTGGGCAGG TAAGTATCAA 901 GGTTACAAGA CAGGTTTAAG GAGACCAATA GAAACTGGGC TTGTCGAGAC AGAGAAGACT 961 CTTGCGTTTC TGATAGGCAC CTATTGGTCT TACTGACATC CACTTTGCCT TTCTCTCCAC 1021 AGGTGTCCAC TCCCAGTTCA ATTACAGCTC TTAAGGCTAG AGTACTTAAT ACGACTCACT 1081 ATAGGCTAGC CTCGAGAATT CACGCGTCGA GCATGCATCT AGGGCGGCCA ATTCCGCCCC 1141 TCTCCCTCCC CCCCCCCTAA CGTTACTGGC CGAAGCCGCT TGGAATAAGG CCGGTGTGCG 1201 TTTGTCTATA TGTGATTTTC CACCATATTG CCGTCTTTTG GCAATGTGAG GGCCCGGAAA 1261 CCTGGCCCTG TCTTCTTGAC GAGCATTCCT AGGGGTCTTT CCCCTCTCGC CAAAGGAATG 1321 CAAGGTCTGT TGAATGTCGT GAAGGAAGCA GTTCCTCTGG AAGCTTCTTG AAGACAAACA 1381 ACGTCTGTAG CGACCCTTTG CAGGCAGCGG AACCCCCCAC CTGGCGACAG GTGCCTCTGC 1441 GGCCAAAAGC CACGTGTATA AGATACACCT GCAAAGGCGG CACAACCCCA GTGCCACGTT 1501 GTGAGTTGGA TAGTTGTGGA AAGAGTCAAA TGGCTCTCCT CAAGCGTATT CAACAAGGGG 1561 CTGAAGGATG CCCAGAAGGT ACCCCATTGT ATGGGATCTG ATCTGGGGCC TCGGTGCACA 1621 TGCTTTACAT GTGTTTAGTC GAGGTTAAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC 1681 GTGGTTTTCC TTTGAAAAAC ACGATGATAA GCTTGCCACA ACCCGGGATC CTCTAGAGTC 1741 GACCCGGGCG GCCGCTTCCC TTTAGTGAGG GTTAATGCTT CGAGCAGACA TGATAAGATA 1801 CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA 1861 AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA 1921 CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG ATGTGGGAGG TTTTTTAAAG 1981 CAAGTAAAAC CTCTACAAAT GTGGTAAAAT CCGATAAGGA TCGATCCGGG CTGGCGTAAT 2041 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 2101 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG 2161 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA 2221 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA 2281 GAGCTTTACG GCACCTCGAC CGCAAAAAAC TTGATTTGGG TGATGGTTCA CGTAGTGGGC 2341 CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG 2401 GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT 2461 AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAATATTTA 2521 ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTTCGCC TGATGCGGTA TTTTCTCCTT 2581 ACGCATCTGT GCGGTATTTC ACACCGCATA CGCGGATCTG CGCAGCACCA TGGCCTGAAA 2641 TAACCTCTGA AAGAGGAACT TGGTTAGGTA CCTTCTGAGG CGGAAAGAAC CAGCTGTGGA 2701 ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA 2761 GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA 2821 GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC 2881 CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT 2941 TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG 3001 GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTTGA TTCTTCTGAC ACAACAGTCT 3061 CGAACTTAAG GCTAGAGCCA CCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC 3121 CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA 3181 TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT 3241 GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC 3301 GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT 3361 ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT 3421 ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT 3481 CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT 3541 CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG 3601 GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT 3661 GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG 3721 TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG 3781 CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG 3841 CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG 3901 ACCGACCAAG CGACGCCCAA CCTGCCATCA CGATGGCCGC AATAAAATAT CTTTATTTTC 3961 ATTACATCTG TGTGTTGGTT TTTTGTGTGA ATCGATAGCG ATAAGGATCC GCGTATGGTG 4021 CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC 4081 ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT 4141 GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG 4201 ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC 4261 TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT 4321 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA 4381 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT 4441 TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC 4501 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT 4561 CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT 4621 ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA 4681 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG 4741 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA 4801 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG 4861 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA 4921 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG 4981 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT 5041 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG 5101 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC 5161 CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA 5221 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC 5281 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT 5341 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC 5401 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 5461 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 5521 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT 5581 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 5641 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 5701 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 5761 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 5821 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 5881 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 5941 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 6001 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 6061 CTGGCCTTTT GCTCACATGG CTCGACAGAT CT //
pIRES载体图谱质粒图谱pdf版和pIRES载体序列质粒序列等相关资料下载
pIRES质粒载体应用举例
pIRES2-ZsGreen1
2015-07-30pIRES2-DsRed2
2015-01-12pIRES2-AcGFP1
2015-07-26pIRES2-DsRed-Express2
2015-07-26pIRES2-DsRed-Express
2015-07-26pIRES-EGFP
2015-07-26pIRES-hrGFP-1a
2015-07-26pIRESneo
2015-07-26pIRESneo2
2015-07-26pIRES2-EGFP
2017-06-22
- 上一篇：pIRESneo3
- 下一篇：pIRESpuro3
