| 质粒名称: | pCE-BiFC-VN173 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 22019 |
| 存储实验室: | Chang-Deng Hu |
|
载体骨架基本信息 |
|
| 载体名称: | pPD4983 |
| 载体出品公司: | Fire lab 1995 kit |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 3993 bp |
| 完整质粒大小: | 4515 bp |
| 载体类型: | Worm Expression |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | VN173 |
| 物种来源: | Aequorea Victoria |
| 插入大小: | 522 bp |
| 基因又名: | Venus(1-172) |
| 基因突变: | N/A |
| 标签/融合蛋白: | Myc (N terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | KpnI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | n/a |
| 3' 测序引物 | n/a |
|
文献引用方法 |
|
| 材料和方法部分: | pCE-BiFC-VN173 was a gift from Chang-Deng Hu (爱迪基因 plasmid # 22019) |
| 参考文献部分: | Visualization of protein interactions in living Caenorhabditis elegans using bimolecular fluorescence complementation analysis. Shyu YJ, Hiatt SM, Duren HM, Ellis RE, Kerppola TK, Hu CD. Nat Protoc. 2008 . 3(4):588-96. 10.1038/nprot.2008.16 PubMed 18388940 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCE-BiFC-VN173 4515 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCE-BiFC-VN173
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4515)
AUTHORS Shyu YJ, Hiatt SM, Duren HM, Ellis RE, Kerppola TK, Hu CD
TITLE Visualization of protein interactions in living Caenorhabditis
elegans using bimolecular fluorescence complementation analysis.
JOURNAL Nat Protoc. 2008 . 3(4):588-96.
PUBMED 18388940
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pCE-BiFC-VN173|
FEATURES Location/Qualifiers
source 1..4515
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 136..654
/codon_start=1
/product="N-terminal fragment of mVenus for use in
bimolecular fluorescence complementation (BiFC) (Kodama and
Hu, 2010)"
/label=VN173
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIE"
primer_bind complement(181..202)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(442..461)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind complement(1522..1544)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 1644..1663
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(1741..1759)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 1827..1931
/gene="bla"
/label=AmpR promoter
CDS 1932..2792
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(2150..2169)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2963..3551
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3452..3471
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3705..3722
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 3839..3860
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3875..3905
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3913..3929
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 3918..3940
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 3937..3953
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 3937..3953
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
ORIGIN
1 atggcatcaa tgcagaagct gatctcagag gaggacctgc ttatggccat ggaggcccga
61 attcggtcga ccgagatctc tcgaggtacc ggtggcggag gttctggtgg cggaggttct
121 ggtggcggag gttctatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
181 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
241 ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc
301 gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac
361 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
421 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
481 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
541 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc
601 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgagtaagcg
661 gccgcaggat ccagacatga taagatacga tatctgagct ccgcatcggc cgctgtcatc
721 agatcgccat ctcgcgcccg tgcctctgac ttctaagtcc aattactctt caacatccct
781 acatgctctt tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaaacttctt
841 cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa ttgtgtagat
901 tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt
961 aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta tgtttttttt
1021 acttctgata aatttttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca
1081 tatgttacgt ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat
1141 gacgtcaaat catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa
1201 gtgaaagttt atgaaattaa ttttcctgct tttgcttttt gggggtttcc cctattgttt
1261 gtcaagagtt tcgaggacgg cgtttttctt gctaaaatca caagtattga tgagcacgat
1321 gcaagaaaga tcggaagaag gtttgggttt gaggctcagt ggaaggtgag tagaagttga
1381 taatttgaaa gtggagtagt gtctatgggg tttttgcctt aaatgacaga atacattccc
1441 aatataccaa acataactgt ttcctactag tcggccgtac gggccctttc gtctcgcgcg
1501 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
1561 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
1621 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
1681 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg
1741 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
1801 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
1861 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
1921 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
1981 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
2041 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
2101 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
2161 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
2221 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
2281 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
2341 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
2401 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
2461 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
2521 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
2581 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
2641 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
2701 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
2761 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
2821 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
2881 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
2941 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
3001 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
3061 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
3121 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
3181 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
3241 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
3301 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag
3361 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
3421 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
3481 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
3541 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
3601 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
3661 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
3721 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
3781 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
3841 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
3901 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
3961 acgccaagct tgcatgcctg caggtcgact ctagaggatc accaaaaacg gaacgttgag
4021 ctggacggaa atagtggtaa agtgacatga ttatagtttg aagatttcta atttcacaat
4081 tagagcaaat gttgttcggt atttattttc aacggtattt atactatttt ccaccttttt
4141 ctagaacatt cgagctgctt gttgcaaaag gagggcgact cacattcggt acatggaaaa
4201 gtagtgtaca caataaagag acccagatac attttccgtc tgcgtctctt tgcacccacc
4261 gggagtattt tcaaacgaat gcatctagga ccttctagaa cattctgtaa ggctgcagaa
4321 tgcgggtata taaggaaagc gggctcagag gaagccaaca cgctttgttc tagtgcatct
4381 aaaaaacttc gaaaatcctc atcgggatcc ccgggattgg ccaaaggacc caaaggtatg
4441 tttcgaatga tactaacata acatagaaca ttttcaggag gacccttggc tagcaattgt
4501 acccgcgggc ccacc
//
