| 质粒名称: | MP-R |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 69684 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | CloDF13 |
| 载体出品公司: | David Liu Lab |
| 载体抗性: | 氯霉素 |
| 空载体大小: | 2992 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | dnaQ926 dam seqA ugi AID (opt) |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | E. coli PBAD |
| 标签/融合蛋白: | -- |
| 克隆方法: | Ligation Independent Cloning |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | pBAD-F |
| 3' 测序引物 | CAT-F |
|
文献引用方法 |
|
| 材料和方法部分: | MP-R was a gift from David Liu (Addgene plasmid # 69684 ; http://n2t.net/addgene:69684 ; RRID:Addgene_69684) |
| 参考文献部分: | Development of potent in vivo mutagenesis plasmids with broad mutational spectra. Badran AH, Liu DR. Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425. 10.1038/ncomms9425 PubMed 26443021 |
买家导航
MPR载体基本信息
MPR载体图谱质粒图谱和多克隆位点信息
MPR载体简介
MPR载体序列
LOCUS MP-R 6092 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Untargetted Bacterial Mutagenesis.
ACCESSION .
VERSION .
KEYWORDS MP-R
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6092)
AUTHORS Badran AH, Liu DR
TITLE Development of potent in vivo mutagenesis plasmids with broad
mutational spectra.
JOURNAL Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
PUBMED 26443021
REFERENCE 2 (bases 1 to 6092)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6092
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(39..777)
/direction=LEFT
/label=CloDF13 ori
/note="Plasmids containing the CloDF13 (CDF) origin of
replication can be propagated in E. coli cells that contain
additional plasmids with compatible origins."
terminator 867..912
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(927..1805)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
promoter 1832..2116
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
primer_bind 2039..2058
/label=pBAD Forward
/note="For vectors with E. coli araBAD promoter, forward
primer"
CDS 4365..4619
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 4649..5245
/codon_start=1
/gene="AICDA"
/product="human activation-induced cytidine deaminase
(Dickerson et al., 2003)"
/label=AID
/translation="MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLD
FGYLRNKNGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFLRGNPNL
SLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHERTFKAWE
GLHENSVRLSRQLRRILLPLYEVDDLRDAFRTLGL"
terminator 5272..5303
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
CDS complement(5319..5978)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVTQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
1 cactcggtcg ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca catacaaagt
61 tacccacaga ttccgtggat aagcagggga ctaacatgtg aggcaaaaca gcagggccgc
121 gccggtggcg tttttccata ggctccgccc tcctgccaga gttcacataa acagacgctt
181 ttccggtgca tctgtgggag ccgtgaggct caaccatgaa tctgacagta cgggcgaaac
241 ccgacaggac ttaaagatcc ccaccgtttc cggcgggtcg ctccctcttg cgctctcctg
301 ttccgaccct gccgtttacc ggatacctgt tccgcctttc tcccttacgg gaagtgtggc
361 gctttctcat agctcacaca ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg
421 ggctgtaagc aagaactccc cgttcagccc gactgctgcg ccttatccgg taactgttca
481 cttgagtcca acccggaaaa gcacggtaaa acgccactgg cagcagccat tggtaactgg
541 gagttcgcag aggatttgtt tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt
601 ccggctacac tggaaggaca gatttggttg ctgtgctctg cgaaagccag ttaccacggt
661 taagcagttc cccaactgac ttaaccttcg atcaaaccac ctccccaggt ggttttttcg
721 tttacagggc aaaagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
781 tctactgaac cgctctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc
841 agcccaggag gaagaggaca tccggtcaaa taaaacgaaa ggctcagtcg aaagactggg
901 cctttcgttt tagacttagg gaccctttat gacaacttga cggctacatc attcactttt
961 tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc
1021 gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg
1081 gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg
1141 ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc
1201 tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa
1261 gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc
1321 cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct
1381 tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc
1441 gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag
1501 gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg
1561 tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct
1621 cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc
1681 attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa
1741 cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca
1801 gccatacttt tcatactccc accattcaga gaagaaacca attgtccata ttgcatcaga
1861 cattgccgtc actgcgtctt ttactggctc ttctcgctaa cccaaccggt aaccccgctt
1921 attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt
1981 gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat
2041 gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc
2101 tctactgttt ctccataccc gtttttttgg acgcgtacaa ctcaagtctg acataaatga
2161 ccgctatgag cactgcaatt acacgccaga tcgttctcgc taccgcaacc accggtatga
2221 accagatcgg tgcgcactat gaaggccaca agatcattga gattggtgcc gttgaagtgg
2281 tgaaccgtcg cctgacgggc aacaacttcc atgtttatct caaacccgat cggctggtgg
2341 atccggaagc ctttggcgta catggtattg ccgatgaatt tttgctcgat aagcccacgt
2401 ttgccgaagt agccgatgag ttcatggact atattcgcgg cgcggagttg gtgatccata
2461 acgcagcgtt cgatatcggc tttatggact acgagttttc gttgcttaag cgcgatattc
2521 cgaagaccaa tactttctgt aaggtcaccg atagccttgc ggtggcgagg aaaatgtttc
2581 ccggtaagcg caacagcctc gatgcgttat gtgctcgcta cgaaatagat aacagtaaac
2641 gaacgctgca cggggcatta ctcgatgccc agatccttgc ggaagtttat ctggcgatga
2701 ccggtggtca aacgtcgatg gcttttgcga tggaaggaga gacacaacag caacaaggtg
2761 aagcaacaat tcagcgcatt gtacgtcagg caagtaagtt acgcgttgtt tttgcgacag
2821 atgaagagat tgcagctcat gaagcccgtc tcgatctggt gcagaagaaa ggcggaagtt
2881 gcctctggcg agcataattc aacatcagta aaccggacat aacccatgaa gaaaaatcgc
2941 gcttttttga agtgggcagg gggcaagtat cccctgcttg atgatattaa acggcatttg
3001 cccaagggcg aatgtctggt tgagcctttt gtaggtgccg ggtcggtgtt tctcaacacc
3061 gacttttctc gttacatcct tgccgatatc aatagcgacc tgatcagtct ctataacatt
3121 gtgaagatgc gtactgatga gtacgtacag gccgcacgcg agctgtttgt tcccgaaaca
3181 aattgcgccg aggtttacta tcagttccgc gaagagttca acaaaagcca ggatccgttc
3241 cgtcgggcgg tactgttctt atatttgaac cgctacggtt acaacggcct gtgtcgttac
3301 aatctgcgcg gtgagtttaa cgtgccgttc ggccgctaca aaaaacccta tttcccggaa
3361 gcagagttgt atcacttcgc tgaaaaagcg cagaacgcct ttttctattg tgagtcttac
3421 gccgacagca tggcgcgcgc agatgatgca tccgtcgtct attgcgatcc gccttatgca
3481 ccgctgtctg cgaccgccaa ctttacggcg tatcacacaa acagttttac gcttgaacaa
3541 caagcgcatc tggcggagat cgccgaaggt ctggttgagc gccatattcc agtgctgatc
3601 tccaatcacg atacgatgtt aacgcgtgag tggtatcagc gcgcgaagct gcatgtcgtc
3661 aaagttcgac gcagcataag cagcaacggc ggcacacgta aaaaggtgga cgaactgctg
3721 gctttgtaca aaccaggagt cgtttcaccc gcgaaaaaat aattcagcta agacactgca
3781 ctggattaag atgaaaacga tcgaagttga tgacgaactc tacagctata tcgccagcca
3841 cactaagcat atcggcgaga gcgcatccga cattttacgg cgtatgttga aattttccgc
3901 cgcatcacag cctgctgctc cggtgacgaa agaggttcgc gttgcgtcac ctgctatcgt
3961 cgaagcgaag ccggtcaaaa cgattaaaga caaggttcgc gcaatgcgtg aacttctgct
4021 ttcggatgaa tacgcagagc aaaagcgagc ggtcaatcgc tttatgctgc tgttgtctac
4081 actatattct cttgacgccc aggcgtttgc cgaagcaacg gaatcgttgc acggtcgtac
4141 acgcgtttac tttgcggcag atgaacaaac gctgctgaaa aacggtaatc agaccaagcc
4201 gaaacatgtg ccaggcacgc cgtattgggt gatcaccaac accaacaccg gccgtaaatg
4261 cagcatgatc gaacacatca tgcagtcgat gcaattcccg gcggaactga ttgagaaggt
4321 ttgcggaact atctaataat acaaaaacta ggaggaactt caacatgaca aatttatctg
4381 acatcattga aaaagaaaca ggaaaacaac tagtgattca agaatcaatt ctaatgttac
4441 cagaagaagt agaggaagta atcgggaata aaccagaaag tgatatttta gttcatactg
4501 cttatgacga aagtacagat gaaaacgtaa tgctattaac ttcagatgct ccagaataca
4561 aaccttgggc gttagtaatt caagacagta atggagaaaa caagattaaa atgttgtaag
4621 tcgagattaa gtaaaccgga atctgaagat ggactctctg ctgatgaacc gtcgcaaatt
4681 cctgtaccag ttcaaaaacg ttcgttgggc gaaaggtcgt cgtgaaacct acctgtgcta
4741 cgttgttaaa cgtcgtgact ctgcgacctc tttctctctg gacttcggtt acctgcgtaa
4801 caaaaacggt tgccacgttg aactgctgtt cctgcgttac atctctgact gggacctgga
4861 cccgggtcgt tgctaccgtg ttacctggtt cacctcttgg tctccgtgct acgactgcgc
4921 gcgtcacgtt gcggacttcc tgcgtggtaa cccgaacctg tctctgcgta tcttcaccgc
4981 gcgtctgtac ttctgcgaag accgtaaagc ggaaccggaa ggtctgcgtc gtctgcaccg
5041 tgcgggtgtt cagatcgcga tcatgacctt caaagactac ttctactgct ggaacacctt
5101 cgttgaaaac cacgaacgta ccttcaaagc gtgggaaggt ctacacgaaa actctgttcg
5161 tctgtctcgt cagctgcgtc gtatcctgct gccgctgtac gaagttgacg acctgcgtga
5221 cgcgttccgt accctgggtc tgtaaactta attaacggca ctcctcagcc aagtcaaaag
5281 cctccgaccg gaggcttttg actacatgcc catggcgttt acgccccgcc ctgccactca
5341 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
5401 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
5461 atagtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
5521 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
5581 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
5641 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
5701 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg
5761 aactccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
5821 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
5881 taggtacatt gagtaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
5941 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
6001 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
6061 gaacctctta cgtgccaagc caaataggcc gt
//
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