| 质粒名称: | pLH01 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 117261 |
| 存储实验室: | Sheng Yang |
|
载体骨架基本信息 |
|
| 载体名称: | pSIP411, pNZ5319 |
| 载体出品公司: | -- |
| 载体抗性: | 氯霉素低（12.5微克/毫升） |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | -- |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | PorfX-Lp_0640-42, sapK-sappR,Lp_0640-42 (2055 bps), sapK-sappR (2095 bps) |
| 物种来源: | Lactobacillus plantarum WCSF1 |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | ApaI(未破坏) |
| 3' 克隆位点: | EcoRI (未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLH01 was a gift from Sheng Yang (Addgene plasmid # 117261 ; http://n2t.net/addgene:117261 ; RRID:Addgene_117261) |
| 参考文献部分: | Development of a RecE/T-Assisted CRISPR-Cas9 Toolbox for Lactobacillus. Huang H, Song X, Yang S. Biotechnol J. 2019 Jul;14(7):e1800690. doi: 10.1002/biot.201800690. Epub 2019 May 20. 10.1002/biot.201800690 PubMed 30927506 |
买家导航
pLH01载体质粒基本信息
pLH01质粒图谱载体图谱和pLH01载体序列质粒序列多克隆位点信息
pLH01质粒载体简介
pLH01质粒序列载体序列
LOCUS pLH01 9199 bp ds-DNA circular SYN 06-JUN-2019
DEFINITION Genome editing for Lactobacillus plantarum WCSF1.
ACCESSION .
VERSION .
KEYWORDS pLH01
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9199)
TITLE RecE/T-Assisted Genome Editing in Lactobacillus
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9199)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 6, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9199
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind complement(303..336)
/label=lox66
/bound_moiety="Cre recombinase"
/note="loxP mutant. Cre-mediated recombination occurs in
the 8-bp core sequence (GCATACAT)."
protein_bind complement(1348..1381)
/label=lox71
/bound_moiety="Cre recombinase"
/note="loxP mutant. Cre-mediated recombination occurs in
the 8-bp core sequence (GCATACAT)."
CDS complement(6616..6627)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
rep_origin 8325..8870
/direction=RIGHT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind 8771..8790
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8987..9004
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 gcgtgttgct ttgattgata gccaaaaagc agcagttgat aaagcaatta ctgatattgc
61 tgaaaaattg taatttataa ataaaaatca ccttttagag gtggtttttt tatttataaa
121 ttattcgttt gatttcgctt tcgatagaac aatcaaagcg agaataagga agataaatcc
181 cataagggcg ggagcagaat gtccgagact aatgtaaatt tgtccaccaa ttaaaggacc
241 gataacgcgc tcgagcctga tagaaacaga agccactgga gcacgtttaa acaatttaaa
301 tctaccgttc gtataatgta tgctatacga agttatgaca atgtcttagg cgttaaggtc
361 gttttagccg atggtcgcga agttaagtaa ggtaccatgc agtttaaatt cggtcctcgg
421 gatatgataa gattaatagt tttagctatt aatctttttt tatttttatt taagaatggc
481 ttaataaagc ggttactttg gatttttgtg agcttggact agaaaaaaac ttcacaaaat
541 gctatactag gtagataaaa atttaggagg catatcaaat gaactttaat aaaattgatt
601 tagacaattg gaagagaaaa gagatattta atcattattt gaaccaacaa acgactttta
661 gtataaccac agaaattgat attagtgttt tataccgaaa cataaaacaa gaaggatata
721 aattttaccc tgcatttatt ttcttagtga caagggtgat aaactcaaat acagctttta
781 gaactggtta caatagcgac ggagagttag gttattggga taagttagag ccactttata
841 caatttttga tggtgtatct aaaacattct ctggtatttg gactcctgta aagaatgact
901 tcaaagagtt ttatgattta tacctttctg atgtagagaa atataatggt tcggggaaat
961 tgtttcccaa aacacctata cctgaaaatg ctttttctct ttctattatt ccatggactt
1021 catttactgg gtttaactta aatatcaata ataatagtaa ttaccttcta cccattatta
1081 cagcaggaaa attcattaat aaaggtaatt caatatattt accgctatct ttacaggtac
1141 atcattctgt ttgtgatggt tatcatgcag gattgtttat gaactctatt caggaattgt
1201 cagataggcc taatgactgg cttttataat atgagataat gccgactgta cttggatcct
1261 aaacgcaatt gatgattggt tcggaaggca cgttaggaat cattaccgaa gtaatcgtta
1321 aactgttgcc gattccgcta gggacccata acttcgtata atgtatgcta tacgaacggt
1381 acagaccggg gtcgaccctt caatagagtt cttaacgtta atccgaaaaa aactaacgtt
1441 aatattaaaa aataagatcc gcttgtgaat tatgtataat ttgattagac taaagaatag
1501 gagaaagtat gatgatattt aaaaaacttt ctcgttaaga taggttgttg gtgagcatgt
1561 tatatacgga tgtatcggtt tccttaatgc aaaattttgt tgctatctta ttaatttttc
1621 tattatatag atatattcaa agaaagataa catttaaacg gatcatatta gatattttaa
1681 tagcgattat tttttcaata ttatatctgt ttatttcaga tgcgtcatta cttgtaatgg
1741 tattaatgcg attagggtgg cattttcatc aacaaaaaga aaataagata aaaacgactg
1801 atacagctaa tttaattcta attatcgtga tccagttatt gttagttgcg gttgggacta
1861 ttattagtca gtttaccata tcgattatca aaagtgattt cagccaaaat atattgaaca
1921 atagtgcaac agatataact ttattaggta ttttctttgc tgttttattt gacggcttgt
1981 tctttatatt attgaagaat aagcggactg aattacaaca tttaaatcaa gaaatcattg
2041 aattttcgtt agaaaaacaa tattttatat ttatatttat tttatttata gtaatagaaa
2101 ttattttagc agttgggaat cttcaaggag taacagccac gatattatta accattatca
2161 ttattttttg tgtccttatc gggatgactt tttggcaagt gatgcttttt ttgaaggctt
2221 attcgattcg ccaagaagcc aatgaccaat tggtccggaa tcaacaactt caagattatc
2281 tagtcaatat cgaacagcag tacaccgaat tacggcgatt taagcatgat tatcaaaaca
2341 tcttattatc gttggagagt tttgccgaaa agggcgatca gcaacagttt aaggcgtatt
2401 accaagaatt attagcacaa cggccaattc aaagtgaaat ccaaggggca gtcattgcac
2461 aactcgacta cttgaaaaat gatcctattc gaggattagt cattcaaaag tttttggcag
2521 ccaaacaggc tggtgttact ttaaaattcg aaatgaccga accaatcgaa ttagcaaccg
2581 ctaatctatt aacggttatt cggattatcg gtattttatt agacaatgcg attgaacaag
2641 ccgttcaaga aaccgatcaa ttggtgagtt gtgctttctt acaatctgat ggtttaatcg
2701 aaattacgat tgaaaatacg gccagtcaag ttaagaatct ccaagcattt tcagagttag
2761 gctattcaac gaaaggcgct ggtcggggga ctggtttagc taatgtgcag gatttgattg
2821 ccaaacaaac caatttattc ttagaaacac agattgaaaa tagaaagtta cgacagacat
2881 tgatgattac ggaggaaact taatttgtat cccgtttatt tattagagga tgatttacag
2941 caacaagcga tttatcagca aattatcgcg aatacgatta tgattaacga atttgcaatg
3001 actttaacat gcgctgccag tgatactgag acattgttgg cggcaattaa ggatcagcaa
3061 cgaggtttat tctttttgga tatggaaatt gaggataacc gccaagccgg tttagaagtg
3121 gcaactaaga ttcggcagat gatgccgttt gcgcaaattg tcttcattac aacccacgag
3181 gaactgacat tattaacgtt agaacgaaaa atagcgcctt tagattacat tctcaaggac
3241 caaacaatgg ctgaaatcaa aaggcaattg attgatgatc tattgttagc tgagaagcaa
3301 aacgaggcgg cagcgtatca ccgagaaaat ttatttagtt ataaaatagg tcctcgcttt
3361 ttctcattac cattaaagga agttgtttat ttatatactg aaaaagaaaa tccgggtcat
3421 attaatttgt tagccgttac cagaaaggtt acttttccag gaaatttaaa tgcgctggaa
3481 gcccaatatc caatgctctt tcggtgtgat aaaagttact tagttaacct atctaatatt
3541 gccaattatg acagtaaaac acggagttta aaatttgtag atggcagtga ggcaaaagtc
3601 tcgttccgga aatcacggga actagtggcc aaattaaaac aaatgatgta gcgcctgcag
3661 cacgccaaat gatcccagta aaaagccacc cgcatggcgg gtggcttttt attagcccta
3721 gaagggcttc ccacacgcat ttcagcgcct tagtgcctta gtttgtgaat cataggtggt
3781 atagtcccga aatacccgtc taaggaattg tcagataggc ctaatgactg gcttttataa
3841 tatgagataa tgccgactgt actttttaca gtcggttttc taatgtcact aacctgcccc
3901 gttagttgaa gaaggttttt atattacagc tccagatcta ccggtgggcc catattaacg
3961 tttaaccgat aaagttgaac gttaatattt tttttgcgca gaaatggtaa attgaagcat
4021 aatagtcttg taaggtattt agctggctgg cgtaaagtat gctttataaa ataatatata
4081 ggagtatgat tcccatgata ccagcacagg caggtttaaa cgagcattgg cagcaacgta
4141 acgactcacg tgactgggta cttgacgcag ataactattg ctacgatggt gacgagttcg
4201 acaaagcaca gttgtttcaa gattacattg ataacaatga ctttgagcag tgggcgactg
4261 atatgcaggc cgatatgtta agtgccattt gtatcgtcac tttcggttcg actgacgtaa
4321 gtgtgttgta tccagatcaa ggtgaggaac ctaattggca atggttgatt gatgtgtttg
4381 gtcagtcccg tctatgggac gagctactgg tacacatcga cacggacacg atgatgacac
4441 gtctgggcta tcactgggta tcagaggggg aagaagcatg agtaatgagc tagttacgat
4501 ggttaataac aatattgagg atatgaagaa taatgaaggc ttgtcattac cacctgatta
4561 ttcagtaggg aatgcattaa acagtgctta cttgattttg agtgatacgt ctaagggcca
4621 accattactt gataagtgtg accaaggatc agttatcaag gcgttaatga acatggcaat
4681 tcaaggattg agcccagcta aaaaccaatg ctatttcatt ccttatggta accagttagt
4741 catgcagcgt tcctattttg gctcaattag cgttgtaaag cgtctttcaa acgttaagga
4801 tattcaggca caggttgtcc acaaagacga cacgttcaag attggtggta aagatggagt
4861 gttggtggtt aaagagttcg agccaagctt tgagaaccta gataagccaa ttatcggggc
4921 ctttgcatgg atcgaagaca tcaacgggaa ccggacatac acggttatga caaaaaagga
4981 catcgacatc agttggagcc acgctaagac gaagaaggtt caaaacgagt tcccagaaga
5041 gatggctaaa cggactgtaa ttaatcgagc tgctaagttc tatattaatt caagctcaga
5101 caacgattta tttgtaaaag cagttaacga aaccactagc aacgaatatg agaatgacga
5161 aagaaaagac gtaacaccgg ctaagcggtc actagtagct gacgtagcag agaataaagc
5221 cgagaaggta gaatctgccg aaccagctaa agaacccgtt agaacggctg taaaggaggc
5281 atcaagcaat gatcaagaac ctgtcaaagg cgaagtcgac cagcaaaacc tcttcgacaa
5341 cctcggagac tttgacgcca gctaactatt acgatcgctg gacagatcaa tcatttatgt
5401 cagcaacatg gttcaagaag tttttagcct gtgaagcaga agcgttagcc gagcttactg
5461 gtaaatggca acctgttcgt gacgctaagg ccttagtagt tggcaattgg cttcatagtt
5521 attttgaaag caagcaagca cacgaaaagt ttaaagatga gcatccagaa tcaatttcaa
5581 aacgcggccc tactaaaggg caactaaaga gtgattttaa ggtcgctaat gctatgattc
5641 agtcgcttac taatgatcat gacttcaatt tactatatca aggcgataag gaagtaattg
5701 ttactggtga aatcggtggt tatccctgga agggcaagat tgattgcctc aacttgaaac
5761 aaggctactt cgttgatcta aagacgaccg ctgacatata caaggtgtat tggaatccag
5821 aaactcgtga gaaagaatcg tttgtatatg cgtataacta cccacttcag atggcagtct
5881 atcaagagtt gattaagcag caatttggtg tgacgtgtaa gccgtacatc gtggcagtaa
5941 gcaaacagga tccaccagac aagcaggcta ttgatttacc ggagtaccga cttactaatg
6001 ctatgaacca ggtattggaa tctcaacagc atcttcaaga tatcattaaa ggcgaagcag
6061 atcctatcca atgcggacat tgtgcttatt gtcgtagtac caaaaagtta gagagcgtcg
6121 ttagtgcaga cgacttactc atagattgat cgactcgagg aattcggtac cccgggttcg
6181 aaggcgccaa gcttcaaatt acagcacgtg ttgctttgat tgatagccaa aaagcagcag
6241 ttgataaagc aattactgat attgctgaaa aattgtaatt tataaataaa aatcaccttt
6301 tagaggtggt ttttttattt ataaattatt cgtttgattt cgctttcgat agaacaatca
6361 aagcgagaat aaggaagata aatcccataa gggcgggagc agaatgtccg agactaattc
6421 atggatcgat tttttattaa aacgtctcaa aatcgtttct gagacgtttt agcgtttatt
6481 tcgtttagtt atcggcataa tcgttaaaac aggcgttatc gtagcgtaaa agcccttgag
6541 cgtagcgtgg ctttgcagcg aagatgttgt ctgttagatt atgaaagccg atgactgaat
6601 gaaataataa gcgcagcgtc cttctatttc ggttggagga ggctcaaggg agtttgaggg
6661 aatgaaattc cctcatgggt ttgattttaa aaattgcttg caattttgcc gagcggtagc
6721 gctggaaaat ttttgaaaaa aatttggaat ttggaaaaaa atggggggaa aggaagcgaa
6781 ttttgcttcc gtactacgac cccccattaa gaccccccat taagtgccga gtgccaattt
6841 ttgtgccaaa aacgctctat cccaactggc tcaagggttt gaggggtttt tcaatcgcca
6901 acgaatcgcc aacgttttcg ccaacgtttt ttataaatct atatttaagt agctttattg
6961 ttgtttttat gattacaaag tgatacacta attttataaa attatttgat tggagttttt
7021 taaatggtga tttcagaatc gaaaaaaaga gttatgattt ctctgacaaa agagcaagat
7081 aaaaaattaa cagatatggc gaaacaaaaa ggtttttcaa aatctgcggt tgcggcgtta
7141 gctatagaag aatatgcaag aaaggaatca gaacaaaaaa aataagcgaa agctcgcgtt
7201 tttagaagga tacgagtttt cgctacttgt ttttgataag gtaattatat catggctatt
7261 aaaaatacta aagctagaaa ttttggattt ttattatatc ctgactcaat tcctaatgat
7321 tggaaagaaa aattagagag tttgggcgta tctatggctg tcagtccttt acacgatatg
7381 gacgaaaaaa aagataaaga tacatggaat agtagtgatg ttatacgaaa tggaaagcac
7441 tataaaaaac cacactatca cgttatatat attgcacgaa atcctgtaac aatagaaagc
7501 gttaggaaca agattaagcg aaaattgggg aatagttcag ttgctcatgt tgagatactt
7561 gattatatca aaggttcata tgaatatttg actcatgaat caaaggacgc tattgctaag
7621 aataaacata tatacgacaa aaaagatatt ttgaacatta atgattttga tattgaccgc
7681 tatataacac ttgatgaaag ccaaaaaaga gaattgaaga atttactttt agatatagtg
7741 gatgactata atttggtaaa tacaaaagat ttaatggctt ttattcgcct taggggagcg
7801 gagtttggaa ttttaaatac gaatgatgta aaagatattg tttcaacaaa ctctagcgcc
7861 tttagattat ggtttgaggg caattatcag tgtggatata gagcaagtta tgcaaaggtt
7921 cttgatgctg aaacggggga aataaaatga caaacaaaga aaaagagtta tttgctgaaa
7981 atgaggaatt aaaaaaagaa attaaggact taaaagagcg tattgaaaga tacagagaaa
8041 tggaagttga attaagtaca acaatagatt tattgagagg agggattatt gaataaataa
8101 aagcccccct gacgaaagtc gcactgattg gtgtatcatt tcgtttttct ttttgtgcgg
8161 acctgcagat gcgatatcat gcgcatgcaa gcttatcgat gataagctgt caaacatgag
8221 aattacaact tatatcgtat ggggctgact tcaggtgcta catttgaaga gataaattgc
8281 actgaaatct agaaatattt tatctgatta ataagatgat cttcttgaga tcgttttggt
8341 ctgcgcgtaa tctcttgctc tgaaaacgaa aaaaccgcct tgcagggcgg tttttcgaag
8401 gttctctgag ctaccaactc tttgaaccga ggtaactggc ttggaggagc gcagtcacca
8461 aaacttgtcc tttcagttta gccttaaccg gcgcatgact tcaagactaa ctcctctaaa
8521 tcaattacca gtggctgctg ccagtggtgc ttttgcatgt ctttccgggt tggactcaag
8581 acgatagtta ccggataagg cgcagcggtc ggactgaacg gggggttcgt gcatacagtc
8641 cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga gacaaacgcg
8701 gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg
8761 agccgccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc caccactgat
8821 ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggctttg
8881 ccgcggccct ctcacttccc tgttaagtat cttcctggca tcttccagga aatctccgcc
8941 ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag
9001 cgaggaagcg gaatatatcc tgtatcacat attctgctga cgcaccggtg cagccttttt
9061 tctcctgcca catgaagcac ttcactgaca ccctcatcag tgccaacata gtaagccagt
9121 atacactccg ctagcgctga tgtccggcgg tgcttttgcc gttacgcacc accccgtcag
9181 tagctgaaca ggagggaca
//
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