| 质粒名称: | hOC-GFPtpz |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 110207 |
| 存储实验室: | David Rowe |
|
载体骨架基本信息 |
|
| 载体名称: | pUC18 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 2700 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | eGFPtopaz |
| 物种来源: | Aequorea victoria |
| 插入大小: | 720 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | Human osteocalcin (BGLAP) |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Hind3（销毁后未知） |
| 3' 克隆位点: | Hind3（销毁后未知） |
| 5' 测序引物 | M13forward |
| 3' 测序引物 | M13reverse |
|
文献引用方法 |
|
| 材料和方法部分: | hOC-GFPtpz was a gift from David Rowe (Addgene plasmid # 110207 ; http://n2t.net/addgene:110207 ; RRID:Addgene_110207) |
| 参考文献部分: | Visualizing levels of osteoblast differentiation by a two-color promoter-GFP strategy: Type I collagen-GFPcyan and osteocalcin-GFPtpz. Bilic-Curcic I, Kronenberg M, Jiang X, Bellizzi J, Mina M, Marijanovic I, Gardiner EM, Rowe DW. Genesis. 2005 Oct;43(2):87-98. doi: 10.1002/gene.20156. 10.1002/gene.20156 PubMed 16149065 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS hOC-GFPtpz 10852 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses GFP in mature bone cells..
ACCESSION .
VERSION .
KEYWORDS hOC-GFPtpz
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10852)
AUTHORS Bilic-Curcic I, Kronenberg M, Jiang X, Bellizzi J, Mina M,
Marijanovic I, Gardiner EM, Rowe DW
TITLE Visualizing levels of osteoblast differentiation by a two-color
promoter-GFP strategy: Type I collagen-GFPcyan and
osteocalcin-GFPtpz.
JOURNAL Genesis. 2005 Oct;43(2):87-98. doi: 10.1002/gene.20156.
PUBMED 16149065
REFERENCE 2 (bases 1 to 10852)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10852
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 38..60
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 52..69
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 53..69
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS 3998..4717
/codon_start=1
/product="enhanced yellow variant of GFP"
/label=Topaz YFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMRQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4043..4064)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4304..4323)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4651..4672
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 4755..4979
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(8536..8553)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8707..9295)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8787..8806)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9466..10326)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10089..10108
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10327..10431)
/gene="bla"
/label=AmpR promoter
primer_bind 10499..10517
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(10555..10577)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 10677..10696
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
61 acggccagtg ccaagctttc tagtagggct caagtggcgg catctctgtc gaactctctc
121 catccttcat gtcagtatcc aatgtacatc tacttatagc cacttgacag cccccttcct
181 ggggcaaaca ctcatattcc tgagggatac aggtccccaa atttatggct cctggatctc
241 cctggtcttc tcttcccaat accagttagc tgtagcctga aggccctgct gcagcttggg
301 aagggccccg gctgggaggc agtgacaagt ggaaggccgg cagccgctct gctccctact
361 gtccccactc cctctgctct ccccacatca cagactggcc tgggggactg ggcagcaggc
421 gcgaaagtgg tggtgacaaa ggcccccccc cccaagcctg gtgccatctt gcagggcaca
481 gctccgagtt gtgtcttggg cactcccagg gccccctgct gagtcctcag gcaaggcgct
541 gctccactat cgccactcag tcaccatgag gccatgggtc actcctgtct ctgggtcctg
601 gcttccaaat tggctagata atgatcccag ggcctcacca gctctgagat tcagtgctat
661 cctaagagga acaaagcccc ctgctcttaa agccatgccc cacccattcc agctacagac
721 cctcacgttg tttgacatgg gcaatttcca tgtctgtaac tcaactgaaa gtgtacttaa
781 ttcaagaaag tttctcgcta gaaagctctg ttgaggtcag aggattgaag ggccactttg
841 gggacccttg ctgaagatga tgggctctgc tctggggatg cccagcctag gtggtcacag
901 aacccagctt gctataaata cttgccgaat ggtggggact tcctgatgtg ccttcatcct
961 aacatgctct gcagggccca tgacttagcc acagactcca tccatattgt cctgggggaa
1021 accccaggcc tggagtcagg gacagtggag ggcaaggatt tgggttttct tagatcccag
1081 tttgtctgtt gtctcccatt gatttcaggc tctacacaga gaacagaggg agctggttgc
1141 tgtgctgagg gagcctgagt gaggagaccg ccagccccag aagcagaggg cagtcaaggt
1201 caagagcctg tggtccagca tgcctggcct gggcgggcta cctctgagaa cggctgaaat
1261 ggtgcccagt ccatcagcag tgatggaatt tgctggagga ctaggccaga gcaagcctca
1321 ctgccactgt gcctttgggg cacccttggg gttggacata cacccccttt agattcctct
1381 gtttcttcta cctggataat tcttggccat gttctctctt ctctaggttc aggtcagctc
1441 tgcccctccg cccccctcct gctggttccc cagccctttt ccctggccct ggcttggaga
1501 atctgttttc aatctccact gattgccccc ttgctggcca gcccaggggc ctttaccatg
1561 ttctctccac atccgtaaat aaacttcctt cactacactg taatctgtga gcatgcttcc
1621 tctgccttgg cctctcttgg gccaagacct gctcctgggc tcccaaatgc tggctccagt
1681 caaggcttca gtcgtggtcc tctgggagca tccaggctgg ggtggtttgg caaggggctt
1741 actagaccat catgtgcaga gagcagcaga gccccttgta caaaaaccag gacccctggg
1801 ttccctgtgg ataggatgag ctgccaagga gatgtcactc ttggcaccct ccaagaatca
1861 ttacggccgg gtgcagtggc tcatgcttga aatcccagca ctttgggagg ccgaggcggg
1921 tgaatcacct gaggtcagga gttcgagacc agcctggcca acatggtgaa atcctgtctc
1981 tactaaaaat acaaaattag ccaggcatgg tggcatgtgc ctgtaatccc agctactcgg
2041 gaggctgagg caggagaatc acttgaaccc gggaggcgga ggttgcagtc agccaagatt
2101 gcgccactgt actccagcct gggcaacaag agtgaaactc catctcaaaa aaaaaaaaaa
2161 aaaaatacag aattagccag gcatggtggc gtgtgcctgt aatcccagct actcaagagg
2221 ctgaggcagg agaatcactt gaacctggga ggcagaggtt gcagtgagcc gagatcgcac
2281 cattgcactc ctgcctgggc gacagagcga gactccgtct caaaaaaaaa gaaccgtgag
2341 cactttgttt ctcttgcttc ttccacttca ctcttcttca gccagggcag ccaaaggcag
2401 gatgggtgct tcccggatcc accagagggg gaaaggggtt gggcgtgtat gggggcagaa
2461 cttttcccta gtgagagcct tggtgaggtc tgctggaggc acactgggga cacaggcaag
2521 catcaggtgg tcccaacttc ccacccccct cccataaacg gcacttggct cagtctccct
2581 ctgcccacct tgtcaccatg gagctgtggc attcaagcat ccaaatgccc tgcttctgtc
2641 aggcctgccc cgtcgggtgc tttgtttact tgtcaggttg ggcagatggt ctcaagccct
2701 ggttggtggg tgggtgagtg aggagacagg ttagacaggg gaaggtccca ggacatgtct
2761 tccctctctg ggctgacctg aggcagtgga ggctctcagg tgtgggatgg ggttttcagg
2821 ctgggatgtt ctgtaccgta gaggataggc cttcctatta cttatcggag cactacggga
2881 gggcaggtcc ctccccaggg tgtttaacac tggaggctgc agggtcagga ggagaatcgt
2941 ggggccagga gggcagaggc acactccatc ttcgtgctcc tcacaggccc tgcctccctg
3001 cctgctaagg acacagggaa gggggtcccc acctcagtgc ctgcctccct tccctgtgcc
3061 tgtgtacctg gcagtcacag ccacctggcg tgtcccagaa accaaccggc tgacctcatc
3121 tcctgcccgg ccccacctcc attggctttg gcttttggcg tttgtgctgc ccgacccttt
3181 ctcctgtccg gatgcgcagg gcagggcctg agccgtcgag ctgcacccac agcaggctgc
3241 ctttggtgac tcaccgggtg aacgggggca ttgcgaggca tcccctccct gggtttggct
3301 cctgcccacg gggctgacag tagaaatcac aggctgtgag acagctggag cccagctctg
3361 cttgaaccta ttttaggtct ctgatccccg cttcctcttt agactcccct agagctcagc
3421 cagtgctcaa cctgaggctg ggggtctctg aggaagagtg agttggagct gaggggtctg
3481 gggctgtccc ctgagagagg ggccagaggc agtgtcaaga gccgggcagt ctgattgtgg
3541 ctcaccctcc atcactccca ggggcccctg gcccagcagc cgcagctccc aaccacaata
3601 tcctttgggg tttggcctac ggagctgggg cggatgaccc ccaaatagcc ctggcagatt
3661 ccccctagac ccgcccgcac catggtcagg catgcccctc ctcatcgctg ggcacagccc
3721 agagggtata aacagtgctg gaggctggcg gggcaggcca gctgagtcct gagcagcagc
3781 ccagcggatc tcgaggaact gaaaaaccag aaagttaact ggtaagttta gtctttttgt
3841 cttttatttc ggatccggtg gtggtgcaaa tcaaagaact gctcctcagt ggatgttgcc
3901 tttacttcta ggcctgtacg gaagtgttac ttctgctcta aaagctgctg caggtcttag
3961 ttaactaaga ctctagggaa ttgcaccggc cacaaccatg gtgagcaagg gcgaggagct
4021 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
4081 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
4141 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
4201 cgtgcagtgc ttcgcccgct accccgacca catgcgccag cacgacttct tcaagtccgc
4261 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
4321 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
4381 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
4441 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
4501 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
4561 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct
4621 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
4681 cgggatcact ctcggcatgg acgagctgta caagtaactc gctagagtcg gggcggccgg
4741 cccttgcagg aattctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
4801 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
4861 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
4921 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatgga
4981 attcgatatc aagctagcaa ttcgagctcg gtacccgggg atcccctgga gcccaggagg
5041 gaggtgtgtg agctcaatcc ggactgtgac gagttggctg accacatcgg ctttcaggag
5101 gcctatcggc gcttctacgg cccggtctag ggtgtcgctc tgctggcctg gccggcaacc
5161 ccagttctgc tcctctccag gcacccttct ttcctcttcc ccttgccctt gccctgacct
5221 cccagcccta tggatgtggg gtccccatca tcccagctgc tcccaaataa actccagaag
5281 aggaatctgt gggcctgtga gtctgtccag tttatggagt gtgggaggga ggtgtcagga
5341 ggatgggggt gaggaggttt taccttcttc agttctagaa agtgctttcc aaagttttat
5401 ttttttattt gtacctgcct tgttccagaa aacattgaag gtggcttccc aaagtctaac
5461 tagggatacc ccctctagcc taggaccctc ctccccacac ctcaatccac caaaccatcc
5521 ataatgcacc cagataggcc cacccccaaa agcctggaca ccttgagcac acagttatga
5581 ccaggacaga ctcatctcta taggcaaata gctgctggca aactggcatt acctggtttg
5641 tggggatggg ggggcaagtg tgtggcctct cggcctggtt agcaagaagc attcagggta
5701 ggcctaggtt agtcgtgtta gttcttccct gtgctgagca gagacttcca gaagcaccag
5761 aaacggagcc agatgaaagg accccaacac ctccccccgc caacctttga cagaatatag
5821 gggcatcttc agcctggaca cgcatgcatc tcccctctca gaccctcagc acttcttcca
5881 ctcccatcaa gagccccctc acggtccctc tcacactctg ccagtccccc tagacacccc
5941 tcctcttctc tgccctctct cctgtgccct ctctcctcag cccctgttgg ttccaggctg
6001 agatgcgtcc ccacctgatt aggcccaaat ctgggctcct cgtcagcact ggggcctggc
6061 ctctgccccc tccaggacag ggtcagggat ggggccttac tgttgtttgg cctgggtacc
6121 cccctccagt gggccaccct gcagcagagg gcatgtactg ggggcccgaa gcagggtggc
6181 tgtgaaagca gcaataatga gtaggttccc agctgcagcc aagaccagtg tggccactgt
6241 gcctgccagg cccagggcag gttcctgtgt ggccagccag gctctgcgtg atcccatatc
6301 agccagcact gcctccagct ggaagtgggt gcccagcact gcacagatgt ggaataactg
6361 gtggctgtgg cctgtggaga ggatgggcag gtcagagcca cacctttccc agtgcaagtc
6421 accccatcct ctgggcctgc tactccccca taaattgaga ataagaaacc ctgactccca
6481 gagtgactga aaggaatgaa tgagcatgtg tgcatgtggc cccagcacac atgctactag
6541 ttttgctacc tgagaagtgg caggtggagg acccttccag tgatgctctc gttataggaa
6601 gacagggaag tccctcctat aatttgcctc catcctgggg ctgtaatacc catttaccca
6661 gttcattctg tgctctggga aggcatctgc ccctgcctct tcccgggccg ggccaggcgt
6721 gccctcaccg atgtaatcaa agcgtcctgg tgccagcctt tcaggcaggt gggaggcgaa
6781 gaggaagcca gtgagcagcg cgcagaagag atggtagcca tggctggtgc tcagggcctc
6841 ctgcccacag ccgtggcccc tgccccagca cagcccgagc tgtcaaagga aaaccaaggc
6901 tgctggggag ggactcggga gccgaaacat ggggtgtgtc tgggatctgg tgaggacgca
6961 gagcgggcgg gcacagctag gcggggtgct ggaccccaag agaggcggct gggctgaggc
7021 agggactgag ggggcagggc tggaagtggg caccttgagg gtcaggacag gaaagcagga
7081 gtctgggcag gggccctagg cctccttacc cgataaaaga gtgggaggtt gtcgaacagg
7141 aatggatagg cgaaggctcc tgtgcggagg accttactga gcccagggct ttccagctcc
7201 aggaaactgg gaggcgggag gaaaaggccg gtcaccatcg ctgtgcttgg ggtaggggtc
7261 agagaaaggc aagaggactt gggcaaagat ggaatatcag cacagcctgc agctaagaaa
7321 accgttaggg catatgacct cctgtgtaaa ggaccacatc tgtatgtggg gataaagggt
7381 ctgacagagg aagtcctcag tacatggtcc cgatgcctcc agtgctgttc gagagcagtg
7441 aggtgtgtcc tctcctgtgc ctccgccttc cccgtcccca ctggaggggc ctgggaaccc
7501 accgggagta gcaggagagg ccggtgcaca ggaaggagtt gagtgcggcg gcaggcacaa
7561 agaactggtg caggtggccg tgcagccagg aggccggcat ggagtaggcg gcatagggga
7621 aggcgcagcc tgcggtgagg tgggggcgtg cagctgaggc ctgaggggcg cgcccctatg
7681 tcccgcgtcc cacccctcgc cccgctccgg gacgcccaga ccccgcgctc ccgcgcctgt
7741 ccggctcacc cagactgtag aggctgagcg cgccgtagtc gaggaagtag cagatgtggc
7801 gcatgcgggg cgacatggag ctgaaggtgt gcgcgcagca cgacgcgaag gggtagaggc
7861 aggcgggcag caggaagacc agcagcggcc agtggtacgg ctccgcacgg aagccggggc
7921 cgcccgccag cgccaggagc cgccacagga agtacctgcg gcgggcgcgt gctcaggccg
7981 ccgcggaccc ccgcgactgc cgctgcagcc cgtgggcctg gacggtccct gcccgcctgg
8041 gtaccccctc tgagctcaag gccgcggcct ggggcagagc ctcccctcac caggtgggca
8101 ggaagtgagt ccagatgttg accgtctcgt tggtcatctg gaaggagctg aggacacagt
8161 ccaaagccga gctggtgggg cggcggtagc cagacatgat gccatcttcc cagaacacct
8221 agggtagtgg tgggagagga agggcgtcac tgtgcccgga ccctttccct ccatctgaga
8281 tctctggaag cagggtcaaa gaggctgggc tggagatcaa cgtggccccc aagccaaact
8341 aaatctgatc tgatgttacc acaggtccca gtacccacaa cccacaaaac aaataggaaa
8401 ccgaagagcc agattggggt gggatggggg tggagcgcag cacacagggc gctctgggga
8461 aggggactcc agatggaggg ccacaagctt gcatgcctgc aggtcgggtt tgcgtattgg
8521 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
8581 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
8641 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
8701 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
8761 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
8821 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
8881 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
8941 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
9001 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
9061 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
9121 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
9181 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
9241 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
9301 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
9361 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
9421 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
9481 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
9541 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
9601 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
9661 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
9721 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
9781 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
9841 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
9901 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
9961 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
10021 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
10081 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
10141 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
10201 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
10261 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
10321 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
10381 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
10441 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
10501 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
10561 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
10621 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
10681 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
10741 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
10801 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gt
//
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