| 质粒名称: | pCMV-Xist-PA |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 26760 |
| 存储实验室: | Rudolf Jaenisch |
|
载体骨架基本信息 |
|
| 载体名称: | pUHG17-1 |
| 完整质粒大小: | 18958 bp |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Xist |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 1500bp |
| 基因突变: | Xist (a.k.a. A430022B11) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | SacII(未破坏) |
| 3’克隆位点: | SalI (未破坏) |
| 5’测序引物 | n/a |
| 3’测序引物 | n/a |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-Xist-PA was a gift from Rudolf Jaenisch (Addgene plasmid # 26760 ; http://n2t.net/addgene:26760 ; RRID:Addgene_26760) |
| 参考文献部分: | A shift from reversible to irreversible X inactivation is triggered during ES cell differentiation. Wutz A, Jaenisch R. Mol Cell. 2000 Apr . 5(4):695-705. 10.1016/S1097-2765(00)80248-8 PubMed 1 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV-Xist-PA 18958 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCMV-Xist-PA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 18958)
AUTHORS Wutz A, Jaenisch R
TITLE A shift from reversible to irreversible X inactivation is triggered
during ES cell differentiation.
JOURNAL Mol Cell. 2000 Apr . 5(4):695-705.
PUBMED 10882105
REFERENCE 2 (bases 1 to 18958)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..18958
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 381..584
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 581..605
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 15381..15614
/codon_start=1
/gene="UL48"
/product="transcriptional activation domain of herpes
simplex virus protein VP16 (Triezenberg et al., 1988;
Cousens et al., 1989)"
/label=VP16 AD
/translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
intron 15654..16226
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
primer_bind complement(16358..16377)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 16423..16478
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(16477..16496)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(16876..16893)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(17047..17635)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(17127..17146)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(17806..18666)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 18429..18448
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(18667..18771)
/gene="bla"
/label=AmpR promoter
primer_bind 18839..18857
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
661 ccagcctccg cggatcagtt aaaggcgtgc aacggcttgc tccagccatg tttgctcgtt
721 tcccgtggat gtgcggttct tccgtggttt ctctccatct aaggagcttt gggggaacat
781 ttttagttcc cctaccacca agccttatgg cttatttaag aaaacatatc aaaattccac
841 gagatttttg acgttttgat atgttctggt aagatttttt ttttgacatg tcctccatac
901 tttttgatat ttgtaatatt ttcagtcaat ttttcatttt taaggaatat ttctttgttg
961 tgccttttgg ttgatacttg tgtgtgtatg gtggacttac ctttctttca ttgtttatat
1021 attcttgccc atcggggcca cggatacctg tgtgtcctcc ccgccattcc atgcccaacg
1081 gggttttgga tacttacctg ccttttcatt cttttttttt cttattattt ttttttctaa
1141 acttgcccat ctgggctgtg gatacctgct tttattcttt ttttcttctc cttagcccna
1201 tcggggccat ggatacctgc tttttgtaaa aaaaaaaaaa aaaaaaaaaa acctttctcg
1261 gtccatcggg acctcggata cctgcgttta gtcttttttt cccatgccca acggggcctc
1321 ggatacctgc tgttattatt tttttttctt tttcttttgc ccatcggggc tgtggatacc
1381 tgctttaaat tttttttttc acggcccaac ggggcgcttg gtggatggaa atatggtttt
1441 gtgagttatt gcactacctg gaatatctat gcctcttatt tgcgtgtact gttgctgctg
1501 atcgtttggt gctgtgtgag tgaacctatg gcttagaaaa acgactttgc tcttaaactg
1561 agtgggtgtt cagggcgtgg agagcccgcg tccgccatta tggcttctgc gtgatacggc
1621 tattctcgag ccagttacgc caagaattag gacaccgagg agcacagcgg actggataaa
1681 agcaaccaat tgcgctgcgc tagctaaagg ctttctttat atgtgcgggg ttgcgggatt
1741 cgccttgatt tgtggtagca tttgcggggt tgtgctagcc ggaagtagaa agccaaggag
1801 tgctcgtatt agtgtgcggt gttgcgcgga agccgcagag gactagggga tagggctcag
1861 cgtgggtgtg gggattgggc agggtgtgtg tgcatatgga cccctggcgc ggtcccccgt
1921 ggctttaagg gctgctcaga agtctataaa atggcggctc gggggctcca cccgaggctc
1981 gacagcccaa tctttgttct ggtgtgtagc aatggattat aggacattta ggtcgtacag
2041 gaaaagatgg cggctcaagt tcttggtgcg gtataacgca aagggctttg tgtgtcacat
2101 gtcagcttca tgtctgagtt agcctggaga ggtggcacat gctcttgaat gtgtctaaga
2161 tggcggaagt catgtgacct gccctctagt ggtttctttc agtgattttt tttttggcgg
2221 gctttagcta cttggcgggc tttgcccgag ggtacacttg gtgcattatg gtagggtgtg
2281 gttggtccta ccttgtgcca ctcgaagctg aggcaaggct aagtggaagt gttggttgcc
2341 acttgacgta actcgtcaga aatgggcaca agtgtgaaag tgttggtgtt tgcttgactt
2401 ccagttagaa atgtgcatta ttgcttggtg gccaggatgg aattagactg tgatgagtca
2461 ctgtcccata aggacgtgag tttcgcttgg tacttcacgt gtgtctttag tcatcatttt
2521 ttcgaagtgc ctgcccaggt cgggagagcg catgcttgca attctaacac tgaagtgttg
2581 gatgatgtcg gatccgattc gagagaccga ggctgcgggt tcttggtcga tgtaaatcat
2641 tgaaacctca cctattaaaa gaaagaaaag tatctaaggc catttcaagg acatttgact
2701 catccgcttg cgttcatagt ctcttacagt gctctatacg tggcggtgca aactaaaact
2761 cagcccgttc cattcctttg tattgttcag tggctagtct acttacacct tggcctctga
2821 tttagccagc actgatctca agcggttctc taagcctact gggtataagt ggtgactttg
2881 gccagagtca tagtggatca caaatcactg gtgaagaggt agaatcctac cttcttccaa
2941 aatctacccc atgactattg ctggggttgc attttgattt caatgaatat tttggatgcc
3001 aacgacacgt ctgatagtgt gctttgctag tgtttgaatt taaaaccgaa gtgattgttt
3061 tcaaaatgta tttacggatt tgcttacttg ttgaattcat tttaattacc tttagtgaat
3121 tgttactttg gagtccttaa agttttcaat aatttttttg gcagatgata ctcaaattac
3181 ttggcactta aatgtacttt ctttcaaact catccaccga gctactcttc aaatttttaa
3241 gtcttataac acagatactg ttaatgtaaa gtgaacatta tgactggatg tcaggagtat
3301 ttgaggttct ataccagttc aggctttgct tttgttgcta ttgttgatgc tatattgact
3361 aatggtttta cttgtcagca agagccttga attgtaatgc tctgtgtcct ctatcagact
3421 tactgttata atagtaatat taaggcctac atttcaactt tctgtgtgtt cttgccttta
3481 tggcatctag attctcctca agactcagca aatagtgctg ctgctattgc tgccccagcc
3541 ccaggcccag ccccagcccc tgccccagcc ccagccccag cccctgcccc agccccagcc
3601 cctgcccctg ccccagcccc tgccccagcc ccagccccag cccctacccc tgcccctgcc
3661 cctgccccac ccaaccaacc caatccagtc cagccctgcc ccagcccagt cctagcccca
3721 ggcccagata ctttcagacc tatcccaagc ccacttctac ttagagaaat tcgaatcttc
3781 attgattcag tgctaaaatg cagtgtccat cactcagcct ataagactga gacagcccat
3841 ctataccccc tccatactga cttctagagt catggaattt cacttaatgc atagaatcgt
3901 attgctaaaa tgcagtgccc atcactcagc ctataagact gagatagccc atctataccc
3961 cctccatact gacttacaga gtcatggagt ttcacttaat gcatgcagtc ctattgctaa
4021 aatgcagtgc ccataactca gcctataaga ctgagatagc ccatttatac cccatacccc
4081 ctccatactg acttctaggg tcatggaatt tcacttaata catagaatcg tattgctaaa
4141 atgcagtgtc catcactcag tctataagac tgagatatcc ctatgtatac cccatactcc
4201 ctccatactg acttccagag tcatagaatt tcactttgca tacggtccta ttgctaaaat
4261 gcagtgtcca tcactcagtc tataagactg agatatccct atgtataccc catactccct
4321 ccatactgac ttccagagtc atagaatttc actttgcata cggtcctatt gctaaaatgc
4381 agtgcccatc actcagccta taagactgag atagcccatc tataccccct ccatactgac
4441 ttccagagtc atggaatttc acttaatgca tgcagtccta ttgctaaaat gcagtgccca
4501 tcactcagcc tataagactg agatagccca tctatacccc ataccccctc catactgact
4561 tccagagtca tggaatttca cttaatgcat gcagtcctat tgctaaaatg cagtgcccat
4621 cactcagcct ataagactga gatagcccat ctatacccac tccatactga cttccagagt
4681 catggaattt cacttaatgc atgcagtcct attgctaaaa tgcagtgccc atcactcagc
4741 ctataagact gagatagccc atctataccc actccatact gacttccaga gtcatggagt
4801 ttcacttaat gcatgcagtc ctattgctaa aatgcagtgc ccataactca gcctataaga
4861 ctgagatagc ccatttatac cccatacccc ctccatactg acttctaggg tcatggaatt
4921 tcacttaatg catagaatcg tattgctaaa atgcagtgtc cattactcag cctataagac
4981 tgagatatcc ctatgtatac cccatacccc ctccatactg acttccagag acatagaatt
5041 tcactttgca tacggtccta ttgctaaaat gcagtgccca tcactcagcc tataagactg
5101 agatatccct atctataccc tctaccccct ccatactgac ttccagagtc atggaatttc
5161 acataatgta tagatttcta ttgctaaaat gcagtgccca taactcagcc tataagactg
5221 agatagccca tctatacccc ctccatactg agttccagag tcatggaatt tcacttaatg
5281 catagaatcg tattgctaaa atgcagtgcc catcactcag cctataagac tgagcccatc
5341 tataccccat accccctcca tactgacttc cagagtcatg gaatttcact ttgcatacag
5401 tcctacttta cttgtccatg gacaagtaaa caaagaactc ttgtccttca tgttaatcaa
5461 gatacaccaa tcaaacaaga gttttatatc agagacttgc catggaggta tcatctctca
5521 agtctccttt cctttaagga aagaaaacca ttctgtcatt gctgtagtag tcacagtccc
5581 aagtttctaa gcagtgttca gtcgtctttt ctcatgtatt accttgagta ctgaataatt
5641 ctgtcagaaa tattttgtcc attggattag actttagcta gtccagccct gtgtgcattt
5701 agcaaagggg caaacacagg tctgttatca gacagttaaa gtgctcagtc ccaattttca
5761 aggcattggc cattaaaggg ggtagaatac tatatactgt tggcatgctg tcatgggtgc
5821 tatcgcccca ggtcacatct ttctaactga tggagataca tttatttgct catgatattg
5881 tatactagtc tcacatgctt tcttatttca gccaaaaacc tctgcactgg aacattttat
5941 gtggataatc ctgactagga attgagtctt ttctcaaggt cctaatacta cccttgcttt
6001 atgtaaagag ggtgctgatt acttaatgcc tcttacacaa ttgtgcaaaa ttgcagttgt
6061 tcaagtcccc ttctgttagt aaccaagatc ccataccctc ataccctaat gggtgacaat
6121 caagggtgcc aaccaatgag accacttctc tgttctggtc tttctgctgt gctggggaat
6181 caaaccttga gtcttgtgta cgctagtaaa gcactgtcat agagctacag ccccaccgtg
6241 tggtggtttg agagaacagc ctcttatgta gcctgggctg ggcgggactt acaggcattg
6301 ccacctgtaa tgtaaacata tttgtgcctg ttgtgtgcac agctgcattt gtccctcttc
6361 ctaagcattg gataaagaaa ccaaactaag tcaagtcatt ttgttggtaa tcaagaagac
6421 ctttgatctg tcctgttttt aacttccagg ctggcctgga acttagcata taacccaggc
6481 tagccttgag ctcaggatct agcctgcgtt taacaagtgt tggcatatct ggttcctacc
6541 actatgccct gcatgcagtc tttcatattg tgaatgtgca tatgtcattt cactgtagta
6601 atctgcatct ggtgaagact tatttgtatt gcagcagtat ttaagatcct taacatagta
6661 aatgtgcaca gtgttaactc tattgtacat attctcatgt ccacagttgt gccttttaga
6721 tcaggactcc tgtacttagc aaagcaaaga ggctcactaa tataaagctt ctttcatgag
6781 actatagatt gaaacgattc caatacggtc aatggtcctt caaggtaaga cttctgtctc
6841 tgatcattca tatcctcttt gctttatgga attatgtatg tgctgtgcac ttgaaacccc
6901 ttcctcaaac tatttatgta catactggca attttagtag gatcaatttt actcttaact
6961 ttgaagtaca gaagtggtgt tgacctataa ggtcccattt tgtggcttgc taataataat
7021 gactgattgt agtaggcctt ttctgttcac tacagaagga aacctgaaca gcgtaaaact
7081 gtaatggcca taaacatgta ccttgcatat tagtatgcat ttactgcaca catctcattc
7141 catttggata cgatcctact ctcaaaccct tttgcagtac agcaagggtc actaatcttt
7201 tggcttcttc atcttcctgg acactggata aggctgtccc ctcctttcca ctctttaatt
7261 tccaggacta ttactttaaa gacttaatat ttgcataaag gatggggttt ttaattgata
7321 acatgtccct tgaacattaa tgtatataac agggacatga tccattcatt ttaataaaaa
7381 tacttggcca gttaatgtgt aaaattacac ttatccacaa ccttattact tttcggacca
7441 ttgtatctct tgcactcctg caagggatac cgtttatctc ccaaggtccc tgctagtgga
7501 ccattaatat acagtgaatc ttcctttgtc tttgccagta aacaaaggcc atactccttc
7561 gcctttcatt tgcactatat caggatatgc tgatcaacaa ggccgcattc ttttggactg
7621 ttatcatata ttaaatgtat gcgtatgcac tgccacctgc tctgtgcact tgaaaggatc
7681 ccactcactt ccttagcacc ttcagcagga agtgataata agctcaagac tttcatttgg
7741 aaagttcaca tgtctaagca cttctctaag aactactgta ccctcttctc cgctttaaag
7801 cagaaagagg gttgtacgaa gtgctcttca tttggactta agtgcattaa tgcagttagt
7861 tgtccatcat tacctttgga gttggatttt acatccttgt actcttttga caccagaggc
7921 atattaatta tttctgagca cttctcttgt caatattaat ctgtaccctt acacatatga
7981 cctgtgcggc agcaaaggtt ctgaaatgcc taccttttga ctggggctgc tgagtggtag
8041 taactattag taacctcagc atttggatga ttactatgca aaaatgtcaa ggacctgtgt
8101 gctctctttg cataccatca aggctactga gtcccagaat taattgctaa gttatgcgta
8161 tttataacta tgaatgtctg gaatattttg tcccctttac attattgcag aggttgctga
8221 gcccccgaaa ctacccggta ctgtcaatga gcacaggggc tctgacgaat gacctgctct
8281 cttccttaaa ctgattttgg gactcttaat aggcacaatg gcagttctgg atggtttatt
8341 ttctactcca acttgagcaa atcccctgct agtttcccaa tgatataata aagtacagca
8401 gtatgtacac ccaacaatga cccggatttc gacccttttt gcattgcttt aatatataca
8461 atcctaaata gtcacaatct cacactttat agtgttcctt ttgcccggcc tctagtttgt
8521 ccattgacca cttttctgaa tcactaattc tcacaaaccc atcattaagg aagagtttgt
8581 gccctttctc aattccatca tgccatccct tttgcctctt tgtttgaaca gtattgactg
8641 ggcaaagccc ttctcttgac ttaaagtcaa caacaccagt ttactcactt catatggcta
8701 cagtgtctca gttgccttct ccttgctccc actgaacaga gacacctcga attcttacat
8761 tattctgggt aatgttaatt accccaaaca ccctatgtgt cattaataaa ttttggtgta
8821 tttatacact gaatagcaaa agcaggccaa aactaggtgg atgagccttc aatctttaac
8881 ttgcacttct aaattattcc aattccaact gctggcacat tctagggcca ggaaccattc
8941 ttgcctacct ttattaatgc tttattgtgc aaaatattgc aggcaagtag ctcagggagt
9001 tggattgcca ccttttactt ggggctttcc tttacagtat gaactgaaaa ttgtcttcct
9061 gagaaggaag cttagcactt ttctttccat tcttcctcca ggaaggagcc aactgtctgc
9121 ttaagaaact ttaagcccga ttttgtatat tgctactgta caggaccaac tgccagaaaa
9181 gttattgata attttattcc ttaagaaagg catttggatt gcaaggtgga ttgactgtga
9241 gatcattagc ttttgtgaag taaaaatagc catttgtgtc atgtttctga agactaagca
9301 gtgtctcagt gtactgaggg tgatgagtct gtggaaagat cagtgcaact attgcagaat
9361 gtttaagaca agtatctttg cttggtcttt actacaagtt taacaaaacg aaaaagtcaa
9421 tctttgtgtg gcctttagta tgattaactt tttggaagat gacctaagcc ttctaatcat
9481 tatattttgt ctgacattgg tcaccagtcc ttgcttattt ttaaaaggtg actggatgga
9541 ttaaatttga gaacatgtca agtcgccttt gaaaattata taggccatca catttaatta
9601 attcattcta tccaccatta aactctggca ataatttgaa gtagcttgaa aattcctaaa
9661 gtgggaattt attttagaga tgatagaacc tgtttcccca ctttacattt taaaatatgt
9721 ctgccaggat ctaatcattc ctttaaacgt acacttcaaa gagagatttt cctagtaaga
9781 aaagagcttt ctctagtgtg aagggtgctt tgtagccgcc gagtacttag gtcttttttg
9841 ggagctattg tgtatgagtg tatgtatgtg tgtgtgtaca tgcatgttgc tgcgcgcagt
9901 cattcattca catggtgctc agacaacaat gggagctggt tcgtctatct tgtgggtcct
9961 ggagatcaaa gtgagatcat caggcttggc agcaagtgcc ttcaccctcc gcgtgccatc
10021 ttgccatccc gctgctgagt gtttgatatg acattgctga tgaaaataat catcacaaca
10081 gcagttctcc cggcattact gagaaatgat actatttttc tgaggaggat gttcaagtaa
10141 ctcatccagt gcaggatcct gcttgaacta ctgctcctcc gttacatcag actctggctg
10201 tttagactac aggatgaatt tggagtctgt tttgtgctcc tgcctcaaga agaaggattg
10261 cctggattta gaggagtgaa gagtgctgga gagagcccaa agggacaaac aatccctatg
10321 tgagattcaa ggactgccag cagcctatac agctacatta catctcagca gaacttctct
10381 tcaagtcctc gctactctga acaaaaagct tacaggccac atggagaaaa aaagatctcc
10441 ccccagaatt gtgggcttgt tgctttgcag tgctggcgac ctattccctt tgacgatccc
10501 taggtggaga tggggcatga ggatcctcca ggggaatagc tcaccaccac tgggcaacag
10561 gcctagccca gatttcagtg agacgctttc ctgaacccag caaggaagac aaaggctcaa
10621 agaatgccac cctacatcaa agtaggagaa aagctgctgc aatagtggca ctgaccttcc
10681 gaggaagcca ttctgctcta tttggttctc tctccagaag ttaggagaac tttgccagct
10741 gtttacatac ttcaagatgc actgctaccc tactcatgcc atataataca caatgccatc
10801 taccaaatat tacccttccc caaagcagca cagaaaactg ggtcttcagc atgatcaagc
10861 aatgtgaaca cacaaaagga aggcagcttt ataaatgacc cgaggatcaa catgcctgac
10921 tgcagcatct taaaagcaat agaatgagtg tgtattgtgt tgtgtgtcta tttcttgttt
10981 tatgtatcta ttttttcctt ggtctgtgtg tctaattctt tgttacacct atttcttcct
11041 tgctttgtgt gtctatttct tccttgcatt gtgtctaatt ctttgttata tctatttctt
11101 ccttgcttcg tgtgtctgtc ttccttgctt tgtgtctatt tcttccttgc agttgtgtct
11161 aattctttgt tacatctatt tcttccttgc ttttgtgtgt ctttctttct tgcttttgtg
11221 tgtctatttc ttccttgcag ttgtgtctaa ttctttgtta catctatttc ttccttgctt
11281 ttgtgtctat ttcttccttg cattgtgtct aattctttgg catatatatt tcttcattgc
11341 tttgtgtgtc tatgtctcct tgtgttgtct aattcgttgt tgcatctatt tcttccttgc
11401 tttgtgtcta tgtcttcctt gttttgtgta tctacttctt ccttgtgtgt ctaattcttt
11461 gttacatcta tttcttcctt cctttgcatg tctccttctt tcctttgtgt gtcttttctg
11521 tctgcagtgt gccttaccta ttcccatgtt tctcctgcat gttctttctt gcagagcttt
11581 gagctttgtt tcactttctc tggtgcctgt gtggtctgct ttgtcttcac tagctatggc
11641 tctctgtttt atctatctga ttgctatttc tcttagcttt tcttttactc ctgcctttcg
11701 tgactcccct ttgggtcaca tgttgcatgc atccctctct ttttcttgtg ctcaccccac
11761 ttgttctttg ttcaagttct ctttgtcagt ccatttcagt tttctttctg ctgcttctat
11821 ccttagtgaa ttcttgttta catttcttcc ctgcctttct tgggccactt tctctgtttt
11881 cttttgtatt tgtgtctctt tgctattggt ggatttctta tctcagcatc attctgttgc
11941 tttgtgtttg cttgtgtttc tatcttctac tttcctcctt tctgttcact ttgagcattt
12001 catctcttta caagtctgtg tctctcttgt attctaaagt aatcctttct tggatgtttc
12061 tttgtatgta catgtgcgtg tgtgcatgtg tgttatgtgt gtcatgtgtg agaggagctt
12121 catagcccct tcccaatagg tccagaatgt cacccgtgga gccgttcctc acaccagact
12181 gccctgagaa ataatctaag acaaaataca tcattccgtc cggtcaggat tcaagtggct
12241 ctgaagtgaa cgcccaagta gaagacagaa gttttgcgac ttgagattta aaaggaccaa
12301 aatacacaga tggcccgtct tgagctggct ggacagaatg ctgacaaccc aaagaagagg
12361 aactgtttct acaggacacc tgtgacttcc aagagcgggg aactacgtat gtcataagac
12421 acaaaacctg agctaagtcc aagcataaga cctaaggacc caatcctata tggacagaat
12481 atttaagaga taaaggccta tggcccagaa ctctggaagg atatttctat ccttctatcc
12541 ccaagaccaa gaagggaaat tcgaagatga gacctgcccc ccaaccccag catccctttc
12601 catttcttat atttctattt aagctgtctt cacttgagat gtaatttttc attgttgcca
12661 ttgcccataa aggaatacgt ttttagctgg atagtattgt gcaagggtct gttttaaact
12721 gggtcttagc catttgttaa attgttgatg ttttacaact tccatttctc ttcacatctg
12781 ctccacttga gacggaacta aatccagcca gtgtatatag cctgactatt gaaacttccc
12841 taggaataag catgcataca gatatgcata ctgccatcct ccctacctca gaagccctag
12901 gctgacaaga aaaggaaagc atcaggttgt tagggggaaa acaatgtcag gctatctaga
12961 gaaaatataa agagttgttc cagaccaatg agaagaatta gacaagcaat atgcagatgt
13021 gccaaccctc tgagaagcac cagccagtgt caccttcttt ctttgggctt aggtgagcag
13081 ggtatggttt tctaataatg gtttggggac aaaatgaggt ctgaactccc tgctcatagt
13141 agtggccgag taatttggtg catttcacca aaggaactcc tgggtctaat acctaccttt
13201 aaaattaatg atgagagact ctaaggacta cttaacgggc ttaatctttt tcgtgccttc
13261 ctcttcctct gtaagaggga agttaaatga cacaggatga aaaagtaaca tgctcatagc
13321 acattggcaa ttatacatgg ttattatctg aaagtgtaga gcttttccta taaggcatca
13381 gactaagtac ctgaagcttt gtgggttcat ggtcttagtt gcatattcct tagttgcaaa
13441 tccttttcaa aaggtaagaa aaaggcacac tggtctattg cctgtacttg atcaagccct
13501 gatatgaatg ccagggaatg tctgagtaac attaatttcc ttccctgcat attttttgtg
13561 ctgaatacta aggctgtgat gcttcactgt ggtcaccccc aggtaacaag atattaccag
13621 gtaaccagga aacgtatgaa tacgtaaacc atgaagccta ctgtaacttc caagtcagtg
13681 ctgagtatgt attacatagt agctgaagtc tacgcctctg tgtgctatag gcacaaagat
13741 tgctctagga ataacatgct ttgtaaaaac aaatatatga acataacggg gcttgaatga
13801 ataacagtcc atatacttaa ggccagtgtg tttcttctgc tttggtgagg ctcagtaagt
13861 tatattatac caggtagcag aagagaaaac acatggaaac tgattttaaa ctacaaacta
13921 ggtcactaat gcaggtgatt gattacccta ttctgatcac cttctaattt ctgaataccc
13981 atgttcagca ctgggaataa caaaggggga cattaccaca gaactagaat ttacaaaaga
14041 atgcattaaa taaagcatta tacagctatc aattgttcca tgtgtgcaaa tgaatgacta
14101 ctaactacct ctgatgtatc cgatattgtt ttgggtacat gaaatattca tgagtaactg
14161 ccatgaaata agaatgtttg cattccatac tattcataag gaatgagcca atgcttaatt
14221 taatcagtca aaacttgagt gataagggca tgttaataca agaacatttg cccaggtcac
14281 attatggttg tgggtacttt cttaactata aagcagttca gtagtataag acaagacaaa
14341 ttttctatag aaataaagct gcctataaaa taggcatagt ctctacaaaa ttttcattgt
14401 actttttagc ccataatggg aagagtacag ttaacaagct gggtgtggta gcatgtgctc
14461 tgagctgaag caacaggacc acttgagccc agaaattgga ggctagcctg ggaagaccat
14521 aaggtcaatc tcaaacctgg aggctaaata ttgtctccca tgtgtatatt ctctttcatg
14581 ggtactggag agatacacag acgtacattt cagtgtgtcc acacttgaga ataatatgta
14641 cgttggcatt ttatgaactc ggaggtacca tataaatgta acaattcatt ttcttacttg
14701 gtatcaattt ccaggctttt aaaattctgc cacatttatt atactgtgaa aataaagtaa
14761 ataagtaact gtgaaccact gaatatatga agcattcaat acttgatgag tacatactga
14821 atggcagtca tttattacaa aacagtgccc ttgctaggca ctgggatgca aagagcattc
14881 tcattgtcct gtgtatctaa agaaattatg catgagatta atttataatt tgtaaactgc
14941 catatatatg tgtatatatg caatatttgc ctggtgtgca atgactttgc ttttatccca
15001 ggcatgcaca acagatctgt gtggagcttt gtgaagtcta cagttctata aagccgggac
15061 ctaactgttg gctttatcag tgaacagtga ttactttcta agtttcataa tggctgaaac
15121 ttaatcataa tgcttatcac ctaacaccac ctaataataa ttttaccatg ctatgtgttg
15181 agcgaacaca tagattgctt tctagcatta tgtagcactt ataggagtga aatctagacc
15241 aaaacttcaa ttcacttcaa tgaggaaatg aaaacagaaa aaaaaaatgg atttgtgcaa
15301 ggcagtgtgc taaatgttac actgagtgga ctatgctgtc taggatactt cccagatcaa
15361 gcttatcgat accgtcgacg gcccccccga ccgatgtcag cctgggggac gagctccact
15421 tagacggcga ggacgtggcg atggcgcatg ccgacgcgct agacgatttc gatctggaca
15481 tgttggggga cggggattcc ccgggtccgg gatttacccc ccacgactcc gccccctacg
15541 gcgctctgga tatggccgac ttcgagtttg agcagatgtt taccgatgcc cttggaattg
15601 acgagtacgg tgggtagggg gcgcgaggat cctctagagc tgagaacttc agggtgagtt
15661 tggggaccct tgattgttct ttctttttcg ctattgtaaa attcatgtta tatggagggg
15721 gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc cttgtatcac catggaccct
15781 catgataatt ttgtttcttt cactttctac tctgttgaca accattgtct cctcttattt
15841 tcttttcatt ttctgtaact ttttcgttaa actttagctt gcatttgtaa cgaattttta
15901 aattcacttt tgtttatttg tcagattgta agtactttct ctaatcactt ttttttcaag
15961 gcaatcaggg tatattatat tgtacttcag cacagtttta gagaacaatt gttataatta
16021 aatgataagg tagaatattt ctgcatataa attctggctg gcgtggaaat attcttattg
16081 gtagaaacaa ctacaccctg gtcatcatcc tgcctttctc tttatggtta caatgatata
16141 cactgtttga gatgaggata aaatactctg agtccaaacc gggcccctct gctaaccatg
16201 ttcatgcctt cttctctttc ctacagctcc tgggcaacgt gctggttgtt gtgctgtctc
16261 atcattttgg caaagaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg
16321 gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt
16381 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
16441 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
16501 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
16561 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc
16621 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
16681 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
16741 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
16801 gatccctcga ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
16861 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
16921 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
16981 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
17041 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
17101 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
17161 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
17221 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
17281 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
17341 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
17401 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
17461 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
17521 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
17581 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
17641 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
17701 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
17761 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
17821 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
17881 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
17941 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
18001 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
18061 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
18121 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
18181 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
18241 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
18301 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
18361 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
18421 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
18481 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
18541 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
18601 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
18661 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
18721 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
18781 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
18841 taggcgtatc acgaggccct ttcgtcttca ctcgaggagc ttggcccatt gcatacgttg
18901 tatccatatc ataatatgta catttatatt ggctcatgtc caacattacc gccatgtt
//
