| 质粒名称: | pJYS2_crtYf |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 85544 |
| 存储实验室: | Sheng Yang |
|
载体骨架基本信息 |
|
| 载体名称: | pXMJ19 and pTRCmob |
| 载体抗性: | 大观霉素 |
| 载体类型: | 哺乳细胞表达，CRISPR ; Shuttle vector Corynebacterium glutamicum / E. coli |
| 高拷贝/低拷贝: | 未知 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
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| 插入基因名称: | crRNA of crtYf |
| 物种来源: | Synthetic |
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文献引用方法 |
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| 材料和方法部分: | pJYS2_crtYf was a gift from Sheng Yang (爱迪基因 plasmid # 85544) |
| 参考文献部分: | CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum. Jiang Y, Qian F, Yang J, Liu Y, Dong F, Xu C, Sun B, Chen B, Xu X, Li Y, Wang R, Yang S. Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179. 10.1038/ncomms15179 PubMed 28469274 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pJYS2_crtYf 4380 bp ds-DNA circular SYN 10-JAN-2018 DEFINITION Constitutive transcription of crRNA of crtYf, Spectinomycin resistance. ACCESSION . VERSION . KEYWORDS pJYS2_crtYf SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4380) AUTHORS Jiang Y, Qian F, Yang J, Liu Y, Dong F, Xu C, Sun B, Chen B, Xu X, Li Y, Wang R, Yang S TITLE CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum. JOURNAL Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179. PUBMED 28469274 COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT ORIGDB|GenBank COMMENT VNTAUTHORNAME|biofeng.com| COMMENT VNTNAME|pJYS2_crtYf| FEATURES Location/Qualifiers source 1..4380 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 58..143 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" rep_origin 3466..4054 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3955..3974 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 4208..4225 /label=L4440 /note="L4440 vector, forward primer" terminator 4319..4346 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" ORIGIN 1 tcctgcccgc caccctccgg gccgttgctt cgcaacgttc aaatccgctc ccggcggatt 61 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt 121 cgactgagcc tttcgtttta tttaaatttc ctgccctatg gttgcctgat ctacaacagt 181 agaaattcgg atccattata cctaggactg agctagctgt caatctagac tcgaggagat 241 ttttgagggg gagggagtcg aggaagagcc agagcagaag gcgggggaac cgttctctgc 301 cgacagcgtg agccccctta aaaatcaggc cggggaggaa ccggggaggg atcagagcta 361 ggagcgagac accctaaagg gggggaaccg ttttctgctg acggtgtttc gtttattagt 421 tttcagcccg tggatagcgg agggtgaggg caagtgagag ccagagcaag gacgggaccc 481 ctaaaggggg gaaccgtttt ctgctgacgg tgtttcgttt attagttttc agcccgtgga 541 cggccgcgtt tagcttccat tccaagtgcc tttctgactt gttggatgcg cctttcactg 601 acacctagtt cgcctgcaag ctcacgagtc gagggatcag caaccgattg agaacgggca 661 tccaggatcg cagttttgac gcgaagttcg agcaactcgc ctgtcatttc tcggcgtttg 721 tttgcttccg ctaatcgctg tcgcgtctcc tgcgcatact tactttctgg gtcagcccat 781 ctgcgtgcat tcgatgtagc tgcgccccgt cgccccatcg tcgctagagc tttccgccct 841 cggctgctct gcgtttccac ccgacgagca gggacgactg gctggccttt agccacgtag 901 ccgcgcacac gacgcgccat cgtcaggcga tcacgcatgg cgggaagatc cggctcccgg 961 ccgtctgcac cgaccgcctg ggcaacgttg tacgccactt catacgcgtc gatgatcttg 1021 gcatctttta ggcgctcacc agcagctttg agctggtatc ccacggtcaa cgcgtggcga 1081 aacgcggtct cgtcgcgcgc tgcgcgctct ggatttgtcc agagcactcg cacgccgtcg 1141 atcaggtcgc cggacgcgtc cagggcgctc ggcaggctcg cgtccaaaat cgctagcgcc 1201 ttggcttctg cggtggcgcg ttgtgccgct tcaatgcggg cgcgtccgct ggaaaagtcc 1261 tgctcaatgt actttttcgg cttctgtgat ccggtcatcg ttcgagcaat ctccattagg 1321 tcggccagcc gatccacacg atcatgctgg cagtgccatt tataggctgt cggatcgtct 1381 gagacgtgca gcggccaccg gctcagccta tgcgaaaaag cctggtcagc gccgaaaaca 1441 cgagtcattt cttccgtcgt tgcagccagc aggcgcatat ttgggctggt tttacctgct 1501 gcggcataca ccgggtcaat gagccagatg agctggcatt tcccgctcag cggattcacg 1561 ccgatccaag ccggcgcttt ttctaggcgt gcccatttct ctaaaatcgc gtagacctgc 1621 gggtttacgt gctcaatctt cccgccggcc tggtggctgg gcacatcgat gtcaagcacg 1681 atcaccgcgg catgttgcgc gtgcgtcagc gcaacgtact ggcaccgcgt cagcgctttt 1741 gagccagccc ggtagagctt tggttgggtt tcgccggtat ccgggttttt aatccaggcg 1801 ctcgcgaaat ctcttgtctt gctgccctgg aagctttcgc gtcccaggtg agcgagcagt 1861 tcgcggcgat cttctgccgt ccagccgcgt gagcgcagcg ctagttcggg gtgggtgtcg 1921 aacagatcgg cggaaaattt ccacgcgcta gctgtgactg tgtcctgcgg atcggctaga 1981 gtcatgtctt gagtgctttc tcccagctga tgactggggg ttagccgacg ccctgtgagt 2041 tcccgctcac ggggcgttca actttttcag gtatttgtgc agcttatcgt gttttcttcg 2101 taaatgaacg cttaactacc ttgttaaacg tggcaaatag gcaggattga tggctttcat 2161 agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca 2221 tttcgaaggg caccaataac tgccttaaaa aaagatctgt acatcgatat catatgcaag 2281 ggtttattgt tttctaaaat ctgattacca attagaatga atatttccca aatattaaat 2341 aataaaacaa aaaaattgaa aaaagtgttt ccaccatttt ttcaattttt ttataatttt 2401 tttaatctgt tatttaaata gtttatagtt aaatttacat tttcattagt ccattcaata 2461 ttctctccaa gataactacg aactgctaac aaaattctct ccctatgttc taatggagaa 2521 gattcagcca ctgcatttcc cgcaatatct tttggtatga ttttacccgt gtccatagtt 2581 aaaatcatac ggcataaagt taatatagag ttggtttcat catcctgata attatctatt 2641 aattcctctg acgaatccat aatggctctt ctcacatcag aaaatggaat atcaggtagt 2701 aattcctcta agtcataatt tccgtatatt cttttatttt ttcgttttgc ttggtaaagc 2761 attatggtta aatctgaatt taattccttc tgaggaatgt atccttgttc ataaagctct 2821 tgtaaccatt ctccataaat aaattcttgt ttgggaggat gattccacgg taccatttct 2881 tgctgaataa taattgttaa ttcaatatat cgtaagttgc ttttatctcc tatttttttt 2941 gaaataggtc taattttttg tataagtatt tctttacttt gatctgtcaa tggttcagat 3001 acgacgacta aaaagtcaag atcactattt ggttttagtc cactctcaac tcctgatcca 3061 aacatgtaag taccaataag gttatttttt aaatgtttcc gaagtatttt tttcacttta 3121 ttaatttgtt cgtatgtatt caaatatatc ctcctcacta ttttgattag tacctatttt 3181 atatccatag ttgttaatta aataaactta atttagttta tttatagatt tcattggctt 3241 ctaaattttt tatctagata ataattattt tagttaattt tattctagat tatatatgat 3301 atgatctttc atttccataa aactaaagta agtgtaaacc tattcattgt tttaaaaata 3361 tctcttgcca gtcacgttac gttattagtt catgaccaaa atcccttaac gtgagttttc 3421 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 3481 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 3541 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 3601 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 3661 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 3721 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 3781 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 3841 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 3901 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 3961 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 4021 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 4081 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 4141 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac 4201 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct 4261 tacgcatctg tgcggtattt cacaccgcat atgaagctta agagtttgta gaaacgcaaa 4321 aaggccatcc gtcaggatgg ccttctgctt aatttgatgc ctggcagttt atggcgggcg //
