出品公司: | CAMBIA |
---|---|
载体名称: | pEarleyGate100; pEarleyGate 100 |
质粒类型: | Gateway载体;植物载体;农杆菌双元表达载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | Gateway |
启动子: | 35S |
载体大小: | 11649 bp |
5' 测序引物及序列: | 35S promoter:CTATCCTTCGCAAGACCCTTC |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 卡那霉素、氯霉素(仅空载体) |
筛选标记: | 草丁膦(磷丝菌素)phosphinothricin、 |
克隆菌株: | DB3.1菌株 |
宿主细胞(系): | 植物细胞、农杆菌 |
备注: | -- |
产品目录号: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
买家导航
pEarleyGate100载体质粒基本信息
pEarleyGate100质粒图谱载体图谱和pEarleyGate100载体序列质粒序列多克隆位点信息
pEarleyGate100质粒载体简介
pEarleyGate100质粒序列载体序列
LOCUS pEarleyGate 100 11649 bp ds-DNA circular SYN 26-JUL-2016 DEFINITION Gateway(R)-compatible plant transformation vector with no encoded tag. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11649) AUTHORS Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS. TITLE Gateway-compatible vectors for plant functional genomics and proteomics. JOURNAL Plant J. 2006;45:616-29. PUBMED 16441352 REFERENCE 2 (bases 1 to 11649) AUTHORS Pikaard Lab TITLE Direct Submission JOURNAL Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4 to Vector NTI(R) format http://www.snapgene.com COMMENT The sequence was corrected by inserting a G at position 7757. COMMENT VNTDATE|659923200| COMMENT VNTDBDATE|776390400| COMMENT VNTNAME|pEarleyGate 100| COMMENT VNTAUTHORNAME|Pikaard Lab| FEATURES Location/Qualifiers misc_feature 1..25 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" terminator 111..363 /label=MAS terminator /note="mannopine synthase terminator" CDS complement(373..924) /codon_start=1 /gene="Streptomyces hygroscopicus bar" /product="phosphinothricin acetyltransferase草丁膦(磷丝菌素) 乙酰转移酶" /label=BlpR /note="confers resistance to bialophos or phosphinothricin" promoter complement(930..1310) /label=MAS promoter /note="mannopine synthase promoter (Velten et al., 1984)" promoter 2306..2651 /label=35S promoter /note="CaMV 35S promoter.strong constitutive promoter from cauliflower mosaic virus" protein_bind 2663..2782 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 2812..2842 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 2896..3555 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" CDS 3897..4202 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." protein_bind complement(4243..4367) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" terminator 4420..5127 /label=OCS terminator /note="octopine synthase terminator" misc_feature 5371..5395 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 6695..7324 /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /label=pVS1 StaA CDS 7753..8826 /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 8892..9086 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" misc_feature 9430..9570 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(9756..10344) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(10431..11225) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" ORIGIN 1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg 61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat 121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat 181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa 241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc 301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc 361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct 421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg 481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg 541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg 601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga 661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc 721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc 781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat 841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc 901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat 961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt 1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc 1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt 1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga 1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa 1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat 1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg 1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata 1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa 1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac 1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa 1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc 1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt 1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac 1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt 1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga 1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat 1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat 2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt 2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac 2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga 2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata 2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc 2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag 2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg 2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg 2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg 2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt 2641 tggagaggac acgctcgaga tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa 2701 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg 2761 taaaacacaa catatccagt catattggcg gccgcattag gcaccccagg ctttacactt 2821 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga 2881 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa 2941 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag 3001 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt 3061 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg 3121 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc 3181 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag 3241 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct 3301 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt 3361 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa 3421 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt 3481 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg 3541 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat 3601 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca 3661 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt 3721 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga 3781 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt 3841 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc 3901 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga 3961 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc 4021 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc 4081 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg 4141 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat 4201 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt 4261 tgtgttttac agcattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata 4321 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgcc taggtgagtc 4381 tagagagtta attaagaccc gggactagtc cctagagtcc tgctttaatg agatatgcga 4441 gacgcctatg atcgcatgat atttgctttc aattctgttg tgcacgttgt aaaaaacctg 4501 agcatgtgta gctcagatcc ttaccgccgg tttcggttca ttctaatgaa tatatcaccc 4561 gttactatcg tatttttatg aataatattc tccgttcaat ttactgattg taccctacta 4621 cttatatgta caatattaaa atgaaaacaa tatattgtgc tgaataggtt tatagcgaca 4681 tctatgatag agcgccacaa taacaaacaa ttgcgtttta ttattacaaa tccaatttta 4741 aaaaaagcgg cagaaccggt caaacctaaa agactgatta cataaatctt attcaaattt 4801 caaaagtgcc ccaggggcta gtatctacga cacaccgagc ggcgaactaa taacgctcac 4861 tgaagggaac tccggttccc cgccggcgcg catgggtgag attccttgaa gttgagtatt 4921 ggccgtccgc tctaccgaaa gttacgggca ccattcaacc cggtccagca cggcggccgg 4981 gtaaccgact tgctgccccg agaattatgc agcatttttt tggtgtatgt gggccccaaa 5041 tgaagtgcag gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc agggcgaatt 5101 ttgcgacaac atgtcgaggc tcagcaggac ctgcaggcat gcaagcttgg cactggccgt 5161 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 5221 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 5281 acagttgcgc agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt 5341 ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag 5401 agaaaagagc gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt 5461 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc 5521 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa 5581 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg 5641 cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga 5701 cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc 5761 gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg 5821 ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac 5881 cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc 5941 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg 6001 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc 6061 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc 6121 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg 6181 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt 6241 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc 6301 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg 6361 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg 6421 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg 6481 agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg 6541 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa 6601 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc 6661 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca 6721 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc 6781 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc 6841 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga 6901 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga 6961 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc 7021 ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac 7081 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg 7141 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac 7201 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc 7261 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat 7321 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt 7381 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg 7441 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta 7501 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa 7561 atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa 7621 tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg 7681 gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc 7741 cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg 7801 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag 7861 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc 7921 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa 7981 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg 8041 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac 8101 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg 8161 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg 8221 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag 8281 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg 8341 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg 8401 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg 8461 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc 8521 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc 8581 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc 8641 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg 8701 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag 8761 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt 8821 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa 8881 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg 8941 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact 9001 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact 9061 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa 9121 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc 9181 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga 9241 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg 9301 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 9361 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 9421 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac 9481 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 9541 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 9601 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 9661 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 9721 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 9781 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 9841 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 9901 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 9961 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 10021 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 10081 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 10141 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 10201 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 10261 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 10321 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 10381 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat 10441 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca 10501 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag 10561 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact 10621 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc 10681 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg 10741 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat 10801 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga 10861 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca 10921 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca 10981 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg 11041 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa 11101 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta 11161 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta 11221 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt 11281 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa 11341 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt 11401 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc 11461 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat 11521 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac 11581 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg 11641 gctggctgg //
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pEarleyGate100质粒载体应用举例
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