| 质粒名称: | 35S:RUBY |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 160908 |
| 存储实验室: | Yunde Zhao |
|
载体骨架基本信息 |
|
| 载体名称: | pHDE |
| 空载体大小: | 9346 bp |
| 完整质粒大小: | 14237 bp |
| 载体类型: | 植物表达 |
| 载体抗性: | 壮观霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | RUBY and CaMV 35S promoter |
| 物种来源: | 花椰菜花叶病毒 RUBY and CaMV 35S promoter |
| 插入大小: | 4892 bp |
| 启动子: | CaMV 35S |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | Gibson克隆 |
| 5’测序引物 | CCTGTCAAACACTGATAGTTTtgagacttttcaacaaagggt |
| 3’测序引物 | GCTTACTCAGTTAGGTCTAGCTTATCTTTAATCATATTCCATAGTCCA |
|
文献引用方法 |
|
| 材料和方法部分: | 35S:RUBY was a gift from Yunde Zhao (Addgene plasmid # 160908 ; http://n2t.net/addgene:160908 ; RRID:Addgene_160908) |
| 参考文献部分: | A reporter for noninvasively monitoring gene expression and plant transformation. He Y, Zhang T, Sun H, Zhan H, Zhao Y. Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1. eCollection 2020. 10.1038/s41438-020-00390-1 PubMed 33024566 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS 35S:RUBY 14335 bp ds-DNA circular SYN 23-SEP-2022
DEFINITION RUBY under the control of CaMV 35S promoter/marker for
transformation.
ACCESSION .
VERSION .
KEYWORDS 35S:RUBY
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14335)
AUTHORS He Y, Zhang T, Sun H, Zhan H, Zhao Y
TITLE A reporter for noninvasively monitoring gene expression and plant
transformation.
JOURNAL Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1.
eCollection 2020.
PUBMED 33024566
REFERENCE 2 (bases 1 to 14335)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 23, 2022 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..14335
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 203..994
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 1240..1828
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1729..1748
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1982..1999
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 2014..2154
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(2080..2099)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2240..2262
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
rep_origin 2498..2692
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(2758..3831)
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
CDS complement(4260..4889)
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
misc_feature 6220..6244
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
promoter 6259..6935
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
primer_bind 6872..6892
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
CDS 8444..8500
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 9335..9391
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
promoter 11598..11777
/label=NOS promoter
/note="nopaline synthase promoter"
promoter 11630..11813
/function="NOS promoter"
/label=NOS promoter
/note="nopaline synthase promoter"
terminator 12928..13180
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(13755..13772)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(13755..13771)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(13764..13786)
/label=M13/pUC Forward
/note="In lacZ gene"
misc_feature 13993..14017
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
ORIGIN
1 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
61 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
121 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
181 ccctaaaaca aagttaaaca tcatggggga agcggtgatc gccgaagtat cgactcaact
241 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
301 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
361 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
421 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
481 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
541 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
601 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
661 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
721 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
781 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
841 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
901 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
961 gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc
1021 gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc
1081 acagccaaac tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct
1141 acacaaattg ggagatatat catgcatgac caaaatccct taacgtgagt tttcgttcca
1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
2041 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
2101 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
2161 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
2221 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
2281 aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc
2341 ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc
2401 cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc
2461 tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt
2521 aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat
2581 atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg
2641 gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag
2701 accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta
2761 ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc
2821 agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt
2881 gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt
2941 cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa
3001 acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt
3061 cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg
3121 ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac
3181 cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt
3241 taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca
3301 gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc
3361 ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta
3421 atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag
3481 ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac
3541 gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc
3601 tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg
3661 ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc
3721 tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc
3781 acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc
3841 tcgggcttgg gggttccagt gccattgcag ggccggcaga caacccagcc gcttacgcct
3901 ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct
3961 tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct
4021 catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg
4081 gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg
4141 cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct
4201 cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact
4261 caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc
4321 tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc
4381 tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca
4441 ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca
4501 tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg
4561 taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc
4621 aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc
4681 acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc
4741 acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc
4801 ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct
4861 ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc
4921 atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc
4981 tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct
5041 tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg
5101 gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg
5161 gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc
5221 ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc
5281 ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac
5341 gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc
5401 gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt
5461 ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat
5521 gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc
5581 tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc
5641 gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag
5701 gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga
5761 aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc
5821 gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta
5881 atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa
5941 acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc
6001 gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg
6061 ttggatcaaa gtactttaaa gtactttaaa gtactttaaa gtactttgat cccgagggga
6121 accctgtggt tggcatgcac atacaaatgg acgaacggat aaaccttttc acgccctttt
6181 aaatatccga ttattctaat aaacgctctt ttctcttagg tttacccgcc aatatatcct
6241 gtcaaacact gatagttttg agacttttca acaaagggta atatcgggaa acctcctcgg
6301 attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac
6361 ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctgccgacag
6421 tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac
6481 cacgtcttca aagcaagtgg attgatgtga acatggtgga gcacgacact ctcgtctact
6541 ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa
6601 gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa
6661 ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta
6721 tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
6781 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct
6841 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
6901 aaggaagttc atttcatttg gagaggacac gctgaaaact agtatggatc atgcgaccct
6961 cgccatgatc ctcgcgatct ggttcatcag cttccacttc atcaagctgc tgttctccca
7021 gcagaccacc aagctgcttc cgccaggacc aaagccgctt ccgatcatcg gcaacatcct
7081 tgaggtgggc aagaagccgc atcggtcctt cgccaacctc gccaagattc acggcccact
7141 catttccctc agactcggct ctgtgaccac catcgttgtg tcctctgccg acgtggccaa
7201 agagatgttc ctcaagaagg atcacccgct ctccaaccgc acgatcccga atagtgttac
7261 agccggcgac caccacaagc tcaccatgtc ttggctcccg gtgtctccga agtggcgcaa
7321 cttccgcaag attaccgccg tgcatctgct ctccccacag agactcgatg cctgccagac
7381 attcaggcac gccaaggtgc agcagctcta cgagtacgtt caagagtgcg cccagaaagg
7441 ccaggccgtg gatattggca aggccgcctt tacgaccagc ctcaacctcc tcagcaagct
7501 gttcttcagc gtcgagctgg cgcaccacaa gtcccatacc agccaagagt tcaaagagct
7561 gatctggaac atcatggaag atataggcaa gccgaactac gccgactact tcccgattct
7621 cggctgcgtt gacccatctg gcattagaag aaggctcgcc tgctccttcg acaagctgat
7681 cgccgtgttc cagggcatca tctgcgagag actcgcccca gattcctcca ccacaactac
7741 caccaccacc gacgacgtgc tcgatgtgct cctccagctg ttcaagcaga acgagctgac
7801 gatgggcgag atcaaccacc tcctcgtgga catcttcgac gccggcaccg ataccacatc
7861 ctccacattc gagtgggtga tgaccgagct gatccgcaat ccagagatga tggaaaaggc
7921 ccaagaggaa atcaagcagg tcctcggcaa ggacaagcag atccaagagt ccgacatcat
7981 caacctgccg tacctccagg cgatcatcaa agagacactc cgcctccatc cgccgaccgt
8041 gttcttgctc ccaagaaagg ccgacaccga tgtcgagctg tacggctaca tcgtgccgaa
8101 ggatgcccag atcctcgtga acctctgggc cattggcagg gacccaaacg cctggcagaa
8161 cgccgatatt ttcagcccag agcgcttcat cggctgcgag atcgatgtta agggccgcga
8221 tttcggcctc cttccatttg gcgctggccg cagaatttgc ccaggcatga atctcgccat
8281 caggatgctc accctcatgc tcgccacact cctccagttc ttcaactgga agctcgaagg
8341 cgacatctcc ccgaaggacc tcgacatgga cgagaagttc ggcattgcgc tccaaaagac
8401 caagccgctc aagctcatcc cgattccgcg ctacggtagc ggagctacca attttagcct
8461 ccttaagcag gcaggtgatg tagaagagaa ccccgggcct atgaagatga tgaacggcga
8521 ggacgccaac gaccagatga tcaaagagtc cttcttcatc acccacggca acccgatcct
8581 caccgtcgag gatacacatc cgctcaggcc gttcttcgag acatggcgcg agaagatttt
8641 ctccaagaag ccgaaggcca tcctcatcat ctccggccac tgggagacag tgaagccaac
8701 cgtgaacgcc gtgcacatca acgacaccat ccacgacttc gacgactacc cagccgccat
8761 gtaccagttc aagtacccag ctccaggcga gccagagctt gcgagaaagg tggaagagat
8821 cctcaagaag tccgggttcg agacagccga gacagaccaa aagaggggcc ttgatcacgg
8881 cgcctgggtt ccactcatgc tcatgtatcc agaggcggac atcccggtgt gccagctctc
8941 agttcagcca catctcgacg gcacctacca ctacaatctc ggcagagccc tcgcgccgct
9001 caagaatgat ggcgtgctca ttattggctc cggcagcgcc acacatccac tcgatgagac
9061 accgcactac ttcgatggtg ttgccccttg ggccgctgcc ttcgattctt ggcttaggaa
9121 ggccctcatc aacggccgct tcgaggaagt gaacatctac gagagcaagg ccccgaactg
9181 gaagctcgcc catccatttc cagagcactt ctacccgctc cacgttgtgc tcggcgctgc
9241 tggtgaaaag tggaaggccg agctgatcca ctcctcctgg gatcatggca cactttgcca
9301 cggctcctac aagttcacct ccgccggatc cggagcaacc aactttagcc tgctcaagca
9361 agcaggagat gttgaggaaa atcctggccc catgaccgcc atcaagatga acaccaacgg
9421 cgagggcgag acacagcaca tcctcatgat cccgttcatg gcgcagggcc acctcaggcc
9481 atttctcgaa ctcgccatgt tcctctacaa gcgctcccac gtgatcatca ccctgctcac
9541 aactccgctc aatgccggct tcctcaggca cctccttcac caccattcct actcctccag
9601 cggcatcagg atcgtcgagc tgccattcaa ctccaccaac cacggactcc caccgggcat
9661 cgagaacacc gataagctca cactcccgct cgtggtgtcc ctcttccatt ccaccatcag
9721 cctcgatccg cacctccgcg attacatctc caggcatttc agcccagcca ggccaccact
9781 ctgcgtgatc catgatgtgt tcctcggctg ggttgaccag gtggccaagg atgtgggctc
9841 tacaggcgtg gtgttcacaa caggcggcgc ttatggcaca tccgcctacg tgtccatctg
9901 gaacgatctc ccgcaccaga actactccga cgaccaagag ttcccgctgc caggcttccc
9961 agagaaccat aagttccgca ggtcccagct ccatcggttc ctcagatatg ccgacggctc
10021 cgacgattgg tccaagtatt tccagccgca gctccgccag tccatgaagt cttttggctg
10081 gctctgcaac tccgtggaag agatcgagac actcggcttc tccatcctcc gcaactacac
10141 caagctgccg atctggggca tcggcccact tattgcttcc ccagtgcagc actcctcctc
10201 cgacaacaat tcaacaggcg ccgagttcgt gcagtggctc agcctcaaag agccggactc
10261 cgtcctctac atctccttcg gctcccagaa cacgatcagc ccgacgcaga tgatggaact
10321 cgctgctggc cttgagtcct ccgagaagcc attcctctgg gtgatcagag ccccgttcgg
10381 cttcgacatc aacgaagaga tgcgcccaga gtggctgcca gagggctttg aggaacgcat
10441 gaaggtgaag aaacagggca agctcgtgta caagctcggc ccgcagcttg agatcctcaa
10501 ccatgaatcc atcggcggct ttctcaccca ctgcggatgg aacagcatcc ttgagtctct
10561 tcgcgagggc gttccgatgc ttggatggcc acttgctgcc gagcaggcct acaacctcaa
10621 gtacctcgaa gatgagatgg gcgtcgcggt tgagcttgct agaggcctcg aaggcgagat
10681 ctccaaagag aaggtcaagc gcatcgtcga gatgatcctt gagcgcaacg agggctccaa
10741 aggctgggag atgaagaatc gcgccgtgga aatgggcaaa aagctcaagg acgccgtgaa
10801 cgaggaaaaa gagctgaagg gctcctccgt gaaggcgatc gacgatttcc tcgacgccgt
10861 catgcaggcc aaacttgagc caagcctcca gtgatagtga atatgaagat gaagatgaaa
10921 tatttggtgt gtcaaataaa aaggttgtgt gcttaagttt gtgttttttt cttggcttgt
10981 tgtgttatga atttgtggct ttttctaata ttaaatgaat gtaacatctc attataatga
11041 ataaacaaat gtttctataa tccattgtga atgttttgtt ggatctcttc tccagcatat
11101 aactactgta tgtgctatgg tatggactat ggaatatgat taaagataag acctaactga
11161 gtaagctagc ttgttcgagt attatggcat tgggaaaact gtttttcttg taccatttgt
11221 tgtgcttgta atttactgtg ttttttattc ggttttcgct atcgaactgt gaaatggaaa
11281 tggatggaga agagttaatg aatgatatgg tccttttgtt cattctcaaa ttaatattat
11341 ttgttttttc tcttatttgt tgtgtgttga atttgaaatt ataagagata tgcaaacatt
11401 ttgttttgag taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta atatgaggag
11461 taaaacacta gatccccaaa caagcttgaa actgaaggcg ggaaacgaca atctgatcat
11521 gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga caagccgttt
11581 tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg ggtttctgga
11641 gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa gtcgcctaag
11701 gtcactatca gctagcaaat atttcttgtc aaaaatgctc cactgacgtt ccataaattc
11761 ccctcggtat ccaattagag tctcatattc actctcaatc caaataatct gcaccggatc
11821 cgctagagga tctcgacctg caagatcccg gggggcaatg agatatgaaa aagcctgaac
11881 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga
11941 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatatagga gggcgtggat
12001 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
12061 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga
12121 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa
12181 ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg
12241 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta
12301 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga
12361 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
12421 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga
12481 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc
12541 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga
12601 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata
12661 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg
12721 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc
12781 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg
12841 ccgatagtgg aaaccgacgc cccagcactc gtccgggatc ttggaggtga tgtaacatga
12901 tcacaagctg atcccccgaa tttccccgat cgttcaaaca tttggcaata aagtttctta
12961 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
13021 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
13081 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
13141 gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattgat cccccctcga
13201 cagcttgcat gccagcttgg gctgcaggtc gacgcgtcaa ttgatatcga tccaggcctc
13261 ccagctttcg tccgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc
13321 attgcccaca caccagaatc ctactaagtt tgagtattat ggcattggaa aagctgtttt
13381 cttctatcat ttgttctgct tgtaatttac tgtgttcttt cagtttttgt tttcggacat
13441 caaaatgcaa atggatggat aagagttaat aaatgatatg gtccttttgt tcattctcaa
13501 attattatta tctgttgttt ttactttaat gggttgaatt taagtaagaa aggaactaac
13561 agtgtgatat taaggtgcaa tgttagacat ataaaacagt ctttcacctc tctttggtta
13621 tgtcttgaat tggtttgttt cttcacttat ctgtgtaatc aagtttacta tgagtctatg
13681 atcaagtaat tatgcaatca agttaagtac agtataggct ttttgtgtcg agggggtacc
13741 gagtcgagga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
13801 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
13861 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgggta ccgagctcga
13921 attcaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct
13981 aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga
14041 ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt
14101 cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag
14161 aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga
14221 ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc cagtggacat
14281 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactg
//
