| 质粒名称: | pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro |
|---|---|
| 出品公司: | addgene |
| 目录编号: | 73037 |
| 存储实验室: | Kazuhiro Oka |
|
载体骨架基本信息 |
|
| 载体名称: | pCDH-CMV-MCS |
| 载体出品公司: | Systembio |
| 空载体大小: | 6227 bp |
| 完整质粒大小: | 8220bp |
| 载体类型: | 哺乳动物表达，慢病毒，荧光素酶 |
| 筛选标记: | Puromycin嘌呤霉素 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Nluc-P2A-copGFP-T2A-Puro |
| 物种来源: | Synthetic |
| 插入大小: | 2019bp |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI(未破坏) |
| 3’克隆位点: | EcoRI(未破坏) |
| 5’测序引物 | GGCACCAAAATCAACGGGAC |
| 3’测序引物 | CAACACCACGGAATTGTCAG |
|
文献引用方法 |
|
| 材料和方法部分: | pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro was a gift from Kazuhiro Oka (Addgene plasmid # 73037 ; http://n2t.net/addgene:73037 ; RRID:Addgene_73037) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro 8220 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Express Nluc and copGFP dual reporter.
ACCESSION .
VERSION .
KEYWORDS pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8220)
TITLE pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8220)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8220
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 234..414
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 458..583
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1076..1309
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1493..1537
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 1798..1915
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2005..2208
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2159..2179
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 2205..2229
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 2293..2805
/codon_start=1
/product="NanoLuc(R) luciferase"
/label=Nluc
/note="human codon-optimized"
/translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
RLCERILA"
CDS 2821..2877
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 2902..3564
/codon_start=1
/product="green fluorescent protein 2 from Pontellina
plumata, also known as ppluGFP2 (Shagin et al., 2004)"
/label=CopGFP
/translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
CDS 3649..3702
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 3703..4302
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(3703..3722)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 4199..4219
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 4315..4903
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4368..4388)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4786..4797)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 4977..5210
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5282..5403
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5319..5338)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5373..5392
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 5422..5557
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5484..5503
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(5595..5611)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5595..5611)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5608..5630)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5619..5635
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5643..5673)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5688..5709
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5826..5843)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5997..6585)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6077..6096)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6756..7616)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7379..7398
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7617..7721)
/gene="bla"
/label=AmpR promoter
primer_bind 7789..7807
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(7845..7867)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 7967..7986
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 8180..8202
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 8194..8211
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 8195..8211
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
2281 tagggatcca ccatggtctt cacactcgaa gatttcgttg gggactggcg acagacagcc
2341 ggctacaacc tggaccaagt ccttgaacag ggaggtgtgt ccagtttgtt tcagaatctc
2401 ggggtgtccg taactccgat ccaaaggatt gtcctgagcg gtgaaaatgg gctgaagatc
2461 gacatccatg tcatcatccc gtatgaaggt ctgagcggcg accaaatggg ccagatcgaa
2521 aaaattttta aggtggtgta ccctgtggat gatcatcact ttaaggtgat cctgcactat
2581 ggcacactgg taatcgacgg ggttacgccg aacatgatcg actatttcgg acggccgtat
2641 gaaggcatcg ccgtgttcga cggcaaaaag atcactgtaa cagggaccct gtggaacggc
2701 aacaaaatta tcgacgagcg cctgatcaac cccgacggct ccctgctgtt ccgagtaacc
2761 atcaacggag tgaccggctg gcggctgtgc gaacgcattc tggcgaagct tggaagcgga
2821 gctactaact tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctatg
2881 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg
2941 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg
3001 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac
3061 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc
3121 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc
3181 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc
3241 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc
3301 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc
3361 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg
3421 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc
3481 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc
3541 ttcaagaccc ccattgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac
3601 ggcaccgccg gacccggctc caccggatct cgcgtcgacg gcagtggaga gggcagagga
3661 agtctgctaa catgcggtga cgtcgaggag aatcctggcc caatgaccga gtacaagccc
3721 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
3781 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
3841 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
3901 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
3961 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
4021 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
4081 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
4141 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
4201 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
4261 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagaattcgt cgacaatcaa
4321 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
4381 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
4441 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
4501 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
4561 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
4621 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
4681 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
4741 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
4801 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
4861 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
4921 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
4981 agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg
5041 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
5101 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
5161 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
5221 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
5281 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
5341 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
5401 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg
5461 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
5521 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca
5581 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
5641 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
5701 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
5761 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
5821 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
5881 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
5941 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
6001 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
6061 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
6121 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
6181 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
6241 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
6301 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
6361 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
6421 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
6481 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
6541 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
6601 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
6661 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
6721 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
6781 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
6841 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
6901 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
6961 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
7021 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
7081 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
7141 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
7201 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
7261 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
7321 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
7381 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
7441 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
7501 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
7561 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
7621 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
7681 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
7741 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
7801 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
7861 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
7921 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
7981 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
8041 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
8101 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
8161 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
