| 出品公司: | -- |
|---|---|
| 载体名称: | pCS105 |
| 质粒类型: | 细菌表达载体；双表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | SP6，T3 |
| 载体大小: | 4131 bp |
| 5' 测序引物及序列: | SP6 Forward: ATTTAGGTGACACTATAG |
| 3' 测序引物及序列: | M13 Reverse: CAGGAAACAGCTATGAC |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 表达菌株: | 原核细胞 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pCS105载体质粒基本信息
pCS105质粒图谱载体图谱和pCS105载体序列质粒序列多克隆位点信息
pCS105质粒载体简介
pCS105质粒序列载体序列
LOCUS Exported File 4131 bp ds-DNA circular SYN 11-七月-2016
DEFINITION Vector that allows high-level transient expression in vertebrate
cells as well as?in vitro transcription/translation.
ACCESSION DQ649431
VERSION .
KEYWORDS pCS105
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4131)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2016-07-11 from SnapGene Viewer 1.5.3
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4131
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 35..53
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
/note="color: #ffffff; direction: RIGHT"
misc_feature 79..148
/note="MCS1"
/note="color: #ffff00"
misc_feature 145..172
/note="stop codons"
/note="stop codons in all three reading frames"
/note="color: #ff0000"
polyA_signal 172..306
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
/note="color: #a6acb3"
misc_feature 375..410
/note="MCS2"
/note="color: #00ffff"
promoter complement(423..441)
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
/note="color: #ffffff; direction: LEFT"
primer_bind complement(462..478)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0; direction: LEFT"
protein_bind 486..502
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/note="color: #31849b"
promoter complement(510..540)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
/note="This reverse directional feature has 3 segments:
???1:?510?..?516?/?#ffffff?/?-10
???2:?517?..?534?/?#ffffff
???3:?535?..?540?/?#ffffff?/?-35"
rep_origin complement(864..1452)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
CDS complement(1623..2483)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/note="This feature has 2 segments:
???1:?1623?..?2414?/?#ccffcc
???2:?2415?..?2483?/?#ccffcc?/?signal?sequence
Cleavage site after base 2414"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2484..2588)
/gene="bla"
/note="AmpR promoter"
/note="color: #ffffff; direction: LEFT"
rep_origin complement(2614..3069)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
/note="color: #ffff00"
promoter 3147..4131
/note="CMV IE94 promoter"
/note="enhancer/promoter region of simian cytomegalovirus
major immediate early transcription unit IE94"
/note="color: #ffffff; direction: RIGHT"
ORIGIN
1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
61 ctacttgttc tttttgcagg atcccatcga ttcgaattcg tcgacaggcc aatctggccg
121 cggccgcaag gcctctcgag cctctagaac tatagtgagt cgtattacgt agatccagac
181 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
241 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
301 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
361 gttttttaat tcgcggcgcg ccgcggcgcc aatgcattgg gcccggtacc cagcttttgt
421 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
481 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
541 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
601 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
661 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
721 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
781 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
841 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
901 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
961 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
1021 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
1081 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
1141 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
1201 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
1261 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
1321 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
1381 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
1441 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
1501 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
1561 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
1621 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
1681 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
1741 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
1801 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
1861 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
1921 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
1981 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
2041 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
2101 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
2161 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
2221 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
2281 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
2341 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
2401 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
2461 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
2521 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
2581 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
2641 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
2701 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
2761 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
2821 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
2881 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
2941 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
3001 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
3061 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
3121 cctcttcgct attacgccag tcgatcgacc atagccaatt caatatggcg tatatggact
3181 catgccaatt caatatggtg gatctggacc tgtgccaatt caatatggcg tatatggact
3241 cgtgccaatt caatatggtg gatctggacc ccagccaatt caatatggcg gacttggcac
3301 catgccaatt caatatggcg gacttggcac tgtgccaact ggggaggggt ctacttggca
3361 cggtgccaag tttgaggagg ggtcttggcc ctgtgccaag tccgccatat tgaattggca
3421 tggtgccaat aatggcggcc atattggcta tatgccagga tcaatatata ggcaatatcc
3481 aatatggccc tatgccaata tggctattgg ccaggttcaa tactatgtat tggccctatg
3541 ccatatagta ttccatatat gggttttcct attgacgtag atagcccctc ccaatgggcg
3601 gtcccatata ccatatatgg ggcttcctaa taccgcccat agccactccc ccattgacgt
3661 caatggtctc tatatatggt ctttcctatt gacgtcatat gggcggtcct attgacgtat
3721 atggcgcctc ccccattgac gtcaattacg gtaaatggcc cgcctggctc aatgcccatt
3781 gacgtcaata ggaccaccca ccattgacgt caatgggatg gctcattgcc cattcatatc
3841 cgttctcacg ccccctattg acgtcaatga cggtaaatgg cccacttggc agtacatcaa
3901 tatctattaa tagtaacttg gcaagtacat tactattgga agtacgccag ggtacattgg
3961 cagtactccc attgacgtca atggcggtaa atggcccgcg atggctgcca agtacatccc
4021 cattgacgtc aatggggagg ggcaatgacg caaatgggcg ttccattgac gtaaatgggc
4081 ggtaggcgtg cctaatggga ggtctatata agcaatgctc gtttagggaa c
//
