| 出品公司: | Addgene |
|---|---|
| 载体名称: | pFGC-pcoCas9 |
| 质粒类型: | 植物载体；RNAi载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 35SPPDK promoter |
| 载体大小: | 13330 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | 2XFLAG、SV40NLS(N ter) |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | 草胺膦、草丁膦 |
| 克隆菌株: | HB101等菌株 |
| 宿主细胞（系）: | 农杆菌/植物细胞 |
| 备注: | -- |
| 产品目录号: | #52256 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
LOCUS Exported 13330 bp ds-DNA circular SYN 27-JUL-2016
DEFINITION Binary plant vector for expressing plant codon-optimized Case9 from
the 35SPPDK promoter.
ACCESSION .
VERSION .
KEYWORDS pFGC-pcoCas9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13330)
AUTHORS Sheen Lab / Addgene #52256
TITLE Direct Submission
JOURNAL Exported Wednesday, July 27, 2016
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pFGC-pcoCas9|
FEATURES Location/Qualifiers
source 1..13330
/organism="synthetic DNA construct"
/lab_host="Plant Cells"
/mol_type="other DNA"
misc_feature 5..145
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin 489..683
/note="pVS1 oriV"
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(749..1822)
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/note="pVS1 RepA"
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
CDS complement(2251..2880)
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/note="pVS1 StaA"
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
misc_feature 4181..4205
/note="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
promoter 4487..4996
/note="35SPPDK promoter"
/note="hybrid promoter consisting of the cauliflower mosaic
virus 35S enhancer fused to the maize C4PPDK basal promoter
(Yoo et al., 2007)"
CDS 5005..5007
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 5008..5055
/codon_start=1
/product="two tandem FLAG(R) epitope tags"
/note="2xFLAG"
/translation="DYKDDDDKDYKDDDDK"
CDS 5062..5082
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/note="SV40 NLS"
/translation="PKKKRKV"
CDS join(5107..6543,6733..9396)
/codon_start=1
/product="Cas9 endonuclease from the Streptococcus pyogenes
Type II CRISPR/Cas system"
/note="pcoCas9"
/note="plant codon-optimized Cas9 gene containing the
potato IV2 intron"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
intron 6544..6732
/note="IV2 intron"
/note="modified second intron of the potato ST-LS1 gene
(Vancanneyt et al., 1990)"
CDS 9397..9444
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/note="nucleoplasmin NLS"
/translation="KRPAATKKAGQAKKKK"
terminator 9453..9705
/note="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
promoter 9946..10326
/note="MAS promoter"
/note="mannopine synthase promoter (Velten et al., 1984)"
CDS 10332..10883
/codon_start=1
/gene="Streptomyces hygroscopicus bar"
/product="phosphinothricin acetyltransferase"
/note="BlpR"
/note="confers resistance to bialophos or phosphinothricin"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
WQLDFSLPVPPRPVLPVTEI"
terminator 10893..11145
/note="MAS terminator"
/note="mannopine synthase terminator"
misc_feature 11231..11255
/note="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA"
CDS 11680..12474
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
rep_origin 12561..13149
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
61 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
121 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
181 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
241 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg
301 ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc
361 gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc
421 gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc
481 cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt
541 aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc
601 caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt
661 tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg
721 caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca
781 ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt
841 actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact
901 ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc
961 ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt
1021 aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc
1081 aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt
1141 agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct
1201 tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt
1261 ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac
1321 ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta
1381 gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc
1441 ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc
1501 ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc
1561 ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc
1621 taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg
1681 ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct
1741 gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc
1801 cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca
1861 gggccggcag gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg
1921 gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc
1981 taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt
2041 cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt
2101 gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg
2161 ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc
2221 ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc
2281 cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc
2341 ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag
2401 ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg
2461 cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag
2521 ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac
2581 gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat
2641 cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat
2701 gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg
2761 gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg
2821 ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat
2881 gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga
2941 cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca
3001 gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca
3061 tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc
3121 tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc
3181 atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg
3241 cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct
3301 caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg
3361 tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag
3421 ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt
3481 cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc
3541 cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct
3601 cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc
3661 tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc
3721 ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca
3781 gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca
3841 ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct
3901 actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct
3961 gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc
4021 agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg
4081 aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt
4141 taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc
4201 tgtcaaacac tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc
4261 tgctctagca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
4321 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
4381 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttaggcctt
4441 actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
4501 aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca
4561 aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg
4621 ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacaagga
4681 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata
4741 tctccactga cgtaagggat gacgcacaat cccactatcc ttcgccccaa gcttgggccc
4801 aagcttgggt cgcgccccac ggatggtata agaataaagg cattccgcgt gcaggattca
4861 cccgttcgcc tctcaccttt tcgctgtact ctctcgccac acacaccccc tctccagctc
4921 cgttggagct ccggacagca gcaggcgcgg ggcggtcacg tagtaagcag ctctcggctc
4981 cctctcccct tgctccgtgg atccatggat tacaaggatg atgatgataa ggattacaag
5041 gatgatgatg ataagatggc tccaaagaag aagagaaagg ttggaatcca cggagttcca
5101 gctgctgata agaagtactc tatcggactt gacatcggaa ccaactctgt tggatgggct
5161 gttatcaccg atgagtacaa ggttccatct aagaagttca aggttcttgg aaacaccgat
5221 agacactcta tcaagaagaa ccttatcggt gctcttcttt tcgattctgg agagaccgct
5281 gaggctacca gattgaagag aaccgctaga agaagataca ccagaagaaa gaacagaatc
5341 tgctaccttc aggaaatctt ctctaacgag atggctaagg ttgatgattc tttcttccac
5401 agacttgagg agtctttcct tgttgaggag gataagaagc acgagagaca cccaatcttc
5461 ggaaacatcg ttgatgaggt tgcttaccac gagaagtacc caaccatcta ccaccttaga
5521 aagaagttgg ttgattctac cgataaggct gatcttagac ttatctacct tgctcttgct
5581 cacatgatca agttcagagg acacttcctt atcgagggag accttaaccc agataactct
5641 gatgttgata agttgttcat ccagcttgtt cagacctaca accagctttt cgaggagaac
5701 ccaatcaacg cttctggagt tgatgctaag gctatccttt ctgctagact ttctaagtct
5761 cgtagacttg agaaccttat cgctcagctt ccaggagaga agaagaacgg acttttcgga
5821 aaccttatcg ctctttctct tggacttacc ccaaacttca agtctaactt cgatcttgct
5881 gaggatgcta agttgcagct ttctaaggat acctacgatg atgatcttga taaccttctt
5941 gctcagatcg gagatcagta cgctgatctt ttccttgctg ctaagaacct ttctgatgct
6001 atccttcttt ctgacatcct tagagttaac accgagatca ccaaggctcc actttctgct
6061 tctatgatca agagatacga tgagcaccac caggatctta cccttttgaa ggctcttgtt
6121 agacagcagc ttccagagaa gtacaaggaa atcttcttcg atcagtctaa gaacggatac
6181 gctggataca tcgatggagg agcttctcag gaggagttct acaagttcat caagccaatc
6241 cttgagaaga tggatggaac cgaggagctt cttgttaagt tgaacagaga ggatcttctt
6301 agaaagcaga gaaccttcga taacggatct atcccacacc agatccacct tggagagctt
6361 cacgctatcc ttcgtagaca ggaggatttc tacccattct tgaaggataa cagagagaag
6421 atcgagaaga tccttacctt cagaatccca tactacgttg gaccacttgc tagaggaaac
6481 tctcgtttcg cttggatgac cagaaagtct gaggagacca tcaccccttg gaacttcgag
6541 gaggtaagtt tctgcttcta cctttgatat atatataata attatcatta attagtagta
6601 atataatatt tcaaatattt ttttcaaaat aaaagaatgt agtatatagc aattgctttt
6661 ctgtagttta taagtgtgta tattttaatt tataactttt ctaatatatg accaaaattt
6721 gttgatgtgc aggttgttga taagggagct tctgctcagt ctttcatcga gagaatgacc
6781 aacttcgata agaaccttcc aaacgagaag gttcttccaa agcactctct tctttacgag
6841 tacttcaccg tttacaacga gcttaccaag gttaagtacg ttaccgaggg aatgagaaag
6901 ccagctttcc tttctggaga gcagaagaag gctatcgttg atcttctttt caagaccaac
6961 agaaaggtta ccgttaagca gttgaaggag gattacttca agaagatcga gtgcttcgat
7021 tctgttgaaa tctctggagt tgaggataga ttcaacgctt ctcttggaac ctaccacgat
7081 cttttgaaga tcatcaagga taaggatttc cttgataacg aggagaacga ggacatcctt
7141 gaggacatcg ttcttaccct tacccttttc gaggatagag agatgatcga ggagagactc
7201 aagacctacg ctcacctttt cgatgataag gttatgaagc agttgaagag aagaagatac
7261 accggatggg gtagactttc tcgtaagttg atcaacggaa tcagagataa gcagtctgga
7321 aagaccatcc ttgatttctt gaagtctgat ggattcgcta acagaaactt catgcagctt
7381 atccacgatg attctcttac cttcaaggag gacatccaga aggctcaggt ttctggacag
7441 ggagattctc ttcacgagca catcgctaac cttgctggat ctccagctat caagaaggga
7501 atccttcaga ccgttaaggt tgttgatgag cttgttaagg ttatgggtag acacaagcca
7561 gagaacatcg ttatcgagat ggctagagag aaccagacca cccagaaggg acagaagaac
7621 tctcgtgaga gaatgaagag aatcgaggag ggaatcaagg agcttggatc tcaaatcttg
7681 aaggagcacc cagttgagaa cacccagctt cagaacgaga agttgtacct ttactacctt
7741 cagaacggaa gagatatgta cgttgatcag gagcttgaca tcaacagact ttctgattac
7801 gatgttgatc acatcgttcc acagtctttc ttgaaggatg attctatcga taacaaggtt
7861 cttacccgtt ctgataagaa cagaggaaag tctgataacg ttccatctga ggaggttgtt
7921 aagaagatga agaactactg gagacagctt cttaacgcta agttgatcac ccagagaaag
7981 ttcgataacc ttaccaaggc tgagagagga ggactttctg agcttgataa ggctggattc
8041 atcaagagac agcttgttga gaccagacag atcaccaagc acgttgctca gatccttgat
8101 tctcgtatga acaccaagta cgatgagaac gataagttga tcagagaggt taaggttatc
8161 accttgaagt ctaagttggt ttctgatttc agaaaggatt tccagttcta caaggttaga
8221 gagatcaaca actaccacca cgctcacgat gcttacctta acgctgttgt tggaaccgct
8281 cttatcaaga agtacccaaa gttggagtct gagttcgttt acggagatta caaggtttac
8341 gatgttagaa agatgatcgc taagtctgag caggagatcg gaaaggctac cgctaagtac
8401 ttcttctact ctaacatcat gaacttcttc aagaccgaga tcacccttgc taacggagag
8461 atcagaaaga gaccacttat cgagaccaac ggagagaccg gagagatcgt ttgggataag
8521 ggaagagatt tcgctaccgt tagaaaggtt ctttctatgc cacaggttaa catcgttaag
8581 aaaaccgagg ttcagaccgg aggattctct aaggagtcta tccttccaaa gagaaactct
8641 gataagttga tcgctagaaa gaaggattgg gacccaaaga agtacggagg attcgattct
8701 ccaaccgttg cttactctgt tcttgttgtt gctaaggttg agaagggaaa gtctaagaag
8761 ttgaagtctg ttaaggagct tcttggaatc accatcatgg agcgttcttc tttcgagaag
8821 aacccaatcg atttccttga ggctaaggga tacaaggagg ttaagaagga tcttatcatc
8881 aagttgccaa agtactctct tttcgagctt gagaacggaa gaaagagaat gcttgcttct
8941 gctggagagc ttcagaaggg aaacgagctt gctcttccat ctaagtacgt taacttcctt
9001 taccttgctt ctcactacga gaagttgaag ggatctccag aggataacga gcagaagcag
9061 cttttcgttg agcagcacaa gcactacctt gatgagatca tcgagcaaat ctctgagttc
9121 tctaagagag ttatccttgc tgatgctaac cttgataagg ttctttctgc ttacaacaag
9181 cacagagata agccaatcag agagcaggct gagaacatca tccacctttt cacccttacc
9241 aaccttggtg ctccagctgc tttcaagtac ttcgatacca ccatcgatag aaaaagatac
9301 acctctacca aggaggttct tgatgctacc cttatccacc agtctatcac cggactttac
9361 gagaccagaa tcgatctttc tcagcttgga ggagataaga gaccagctgc taccaagaag
9421 gctggacagg ctaagaagaa gaagtgactg cagatcgttc aaacatttgg caataaagtt
9481 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
9541 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
9601 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
9661 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcaggcc tgtcgacgcc
9721 cgggcggtac cgcgatcgct cgcgacctgc aggcataaag ccgtcagtgt ccgcataaag
9781 aaccacccat aatacccata atagctgttt gccatcgcta ccttaggacc gttatagtta
9841 attaccctgt tatccctatt aattaagagc tcgctacctt aagagaggat atcggcgcgc
9901 ctctagaatt taaatggatc ctacgtactc gaggaattct gagatttttc aaatcagtgc
9961 gcaagacgtg acgtaagtat ccgagtcagt ttttattttt ctactaattt ggtcgtttat
10021 ttcggcgtgt aggacatggc aaccgggcct gaatttcgcg ggtattctgt ttctattcca
10081 actttttctt gatccgcagc cattaacgac ttttgaatag atacgctgac acgccaagcc
10141 tcgctagtca aaagtgtacc aaacaacgct ttacagcaag aacggaatgc gcgtgacgct
10201 cgcggtgacg ccatttcgcc ttttcagaaa tggataaata gccttgcttc ctattatatc
10261 ttcccaaatt accaatacat tacactagca tctgaatttc ataaccaatc tcgatacacc
10321 aaatcgaatc gatgagccca gaacgacgcc cggccgacat ccgccgtgcc accgaggcgg
10381 acatgccggc ggtctgcacc atcgtcaacc actacatcga gacaagcacg gtcaacttcc
10441 gtaccgagcc gcaggaaccg caggagtgga cggacgacct cgtccgtctg cgggagcgct
10501 atccctggct cgtcgccgag gtggacggcg aggtcgccgg catcgcctac gcgggcccct
10561 ggaaggcacg caacgcctac gactggacgg ccgagtcgac cgtgtacgtc tccccccgcc
10621 accagcggac gggactgggc tccacgctct acacccacct gctgaagtcc ctggaggcac
10681 agggcttcaa gagcgtggtc gctgtcatcg ggctgcccaa cgacccgagc gtgcgcatgc
10741 acgaggcgct cggatatgcc ccccgcggca tgctgcgggc ggccggcttc aagcacggga
10801 actggcatga cgtgggtttc tggcagctgg acttcagcct gccggtaccg ccccgtccgg
10861 tcctgcccgt caccgagatt tgactgcaga atcttggact cccatgttgg caaaggcaac
10921 caaacaaaca atgaatgatc cgctcctgca tatggggcgg tttgagtatt tcaactgcca
10981 tttgggctga attgaagaca tgctcctgtc agaaattccg tgatcttact caatattcag
11041 taatctcggc caatatccta aatgtgcgtg gctttatctg tctttgtatt gtttcatcaa
11101 ttcatgtaac gtttgctttt cttatgaatt ttcaaataaa ttatcagatc cgagctcgaa
11161 ttaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
11221 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
11281 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
11341 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
11401 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
11461 gtaacgatga cagagcgttg ctgcctgtga tcaccgcggt ttcaaaatcg gctccgtcga
11521 tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag
11581 gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg
11641 tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca
11701 ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct
11761 cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac
11821 agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg
11881 gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat
11941 ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc
12001 cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata
12061 tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg
12121 aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat
12181 ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc
12241 cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt
12301 gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg
12361 atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc
12421 aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta
12481 gaatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
12541 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
12601 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
12661 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
12721 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
12781 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
12841 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
12901 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
12961 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
13021 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
13081 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
13141 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
13201 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
13261 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
13321 gaagcggaag
//
