| 质粒名称: | pMito.iGABASnFR.mRuby3 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 112179 |
| 存储实验室: | Loren Looger |
|
载体骨架基本信息 |
|
| 载体名称: | pDisplay |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 7554bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | iGABASnFR |
| 标签/融合蛋白: | mRuby3 (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | Unknown (unknown if destroyed) |
| 3’克隆位点: | Unknown (unknown if destroyed) |
|
文献引用方法 |
|
| 材料和方法部分: | pMito.iGABASnFR.mRuby3 was a gift from Loren Looger (Addgene plasmid # 112179 ; http://n2t.net/addgene:112179 ; RRID:Addgene_112179) |
| 参考文献部分: | A genetically encoded fluorescent sensor for in vivo imaging of GABA. Marvin JS, Shimoda Y, Magloire V, Leite M, Kawashima T, Jensen TP, Kolb I, Knott EL, Novak O, Podgorski K, Leidenheimer NJ, Rusakov DA, Ahrens MB, Kullmann DM, Looger LL. Nat Methods. 2019 Aug;16(8):763-770. doi: 10.1038/s41592-019-0471-2. Epub 2019 Jul 15. 10.1038/s41592-019-0471-2 PubMed 31308547 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMito.iGABASnFR.mRuby3 7554 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION GABA Sensor.
ACCESSION .
VERSION .
KEYWORDS pMito.iGABASnFR.mRuby3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7554)
AUTHORS Marvin JS, Shimoda Y, Magloire V, Leite M, Kawashima T, Jensen TP,
Kolb I, Knott EL, Novak O, Podgorski K, Leidenheimer NJ, Rusakov DA,
Ahrens MB, Kullmann DM, Looger LL
TITLE A genetically encoded fluorescent sensor for in vivo imaging of
GABA.
JOURNAL Nat Methods. 2019 Aug;16(8):763-770. doi: 10.1038/s41592-019-0471-2.
Epub 2019 Jul 15.
PUBMED 31308547
REFERENCE 2 (bases 1 to 7554)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7554
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 9..388
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 389..592
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 543..563
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 637..656
/label=T7
/note="T7 promoter, forward primer"
promoter 637..655
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 736..810
/codon_start=1
/product="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
/label=COX8 presequence
/translation="MSVLTPLLLRGLTGSARRLPVPRAK"
primer_bind 1887..1908
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(2011..2032)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3304..3321)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 3310..3534
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin complement(3666..4254)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3746..3765)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
polyA_signal 4583..4630
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
primer_bind 4655..4674
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
CDS complement(4862..5656)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASKPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4973..4992)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 5583..5602
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind complement(5755..5774)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind 6001..6021
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
CDS complement(6104..6964)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6727..6746
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6965..7069)
/gene="bla"
/label=AmpR promoter
rep_origin 7096..7551
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7183..7202)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7393..7414
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 cgcgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
61 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
181 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
241 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
301 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
361 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
421 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
481 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagaa ctctctggct
601 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
661 ccaagcttgg taccgaactc ggatccacta gtaacggccg ccagtgtgct ggaattcggc
721 ttggggatat ccaccatgag cgtgctgacc cccctgctgc tgaggggcct gaccggcagc
781 gccaggaggc tgcccgtgcc cagggccaag atccacagcc tgggcgaccc ccccgtggcc
841 accagatctg aaagcatcaa tttcgtgagc tggggcggta gcacccagga tgcgcagaag
901 caggcctggg ccgacccgtt cagcaaggcc agcggcatta ccgtggtcca ggatgggccc
961 accgactacg gcaaactcaa ggccatggtc gaaagcggca acgtgcagtg ggacgtggtg
1021 gatgtagagg ccgacttcgc cttgcgcgcc gccgccgaag gcctgctcga acccctggat
1081 ttctcggtga tccagcgcga caagatcgac ccgcgcttcg tctccgacca tggcgtgggc
1141 tcgttcctgt tctctttcgt gctcggctac aacgaaggca agctcggagc cagcaagccc
1201 caggactgga ccgcgctgtt cgacaccaag acctaccccg gcaaacgcgc cctctacaaa
1261 tggccgagcc ctggcgtgct cgaactggcc ctgctcgccg acggcgtacc ggccgacaag
1321 ctctacccgc tggacctgga ccgcgccttc aagaaactcg acaccatcaa gaaagacatc
1381 gtctggtggg gcggcggtgc acagtcgcag cagctgctgg cctccggcga agtcagcatg
1441 ggccagttct ggaacggtcg catccacgcc ctgcaagaag acggcgcgcc agtgggcgtg
1501 agctggaagc agaacctggt gatggccgac atcctcgtcg tgcctaaagg cacgaagaac
1561 aaagccgccg cgatgaagtt cctggccagt gccagcagcg ccaaaggcca ggacgacttt
1621 tccgccctga ccgcctatgc tccagtgaac atcgacagtg tgcagcgcct cgaccttgcg
1681 caggtccgga tcaccgccga caagcagaag aacggcatca tggcgaactt caagatccgc
1741 cacaacgtgg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
1801 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgtgctgagc
1861 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
1921 atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat gagcaagggc
1981 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
2041 cacaagttca gcgtgcgcgg cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg
2101 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
2161 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
2221 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcagcttcaa ggacgacggc
2281 acctacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
2341 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
2401 tggaacgcca acctggcacc gaacctgccg accgcctacg tcaaggatca gatcaccctc
2461 gatttcgcct actgggccaa gaacggtccg gccattgcga cacggtggaa tgaatggcta
2521 gtcaaactgc agggctccgg aaatgctgtg ggccaggaca cgcaggagcg tatggtgtct
2581 aagggcgaag agctgatcaa ggaaaatatg cgtatgaagg tggtcatgga aggttcggtc
2641 aacggccacc aattcaaatg cacaggtgaa ggagaaggca ggccgtacga gggagtgcaa
2701 accatgagga tcaaagtcat cgagggagga cccctgccat ttgcctttga cattcttgcc
2761 acgtcgttca tgtatggcag ccgtactttt atcaagtacc cggcagacat tcctgatttc
2821 tttaaacagt cctttcctga gggttttact tgggaaagag ttacgagata cgaagatggt
2881 ggagtcgtca ccgtcacgca ggacaccagc cttgaggatg gcgaactcgt ttacaacgtc
2941 aaagtcagag gggtaaactt tccctccaat ggtcccgtga tgcagaagaa gaccaagggt
3001 tgggagccta atacagagat gatgtatcca gcagatggtg gtctgagggg atacactgat
3061 attgcactga aagttgatgg tggtggccat ctgcattgca atttcgtaac aacttacagg
3121 tcaaaaaaga ccgtcgggaa catcaagatg cccggtgtcc atgccgttga tcaccgcctg
3181 gaaaggatag aggaaagtga caatgaaacg tacgtagtac aacgcgaagt cgcagttgcc
3241 aagtactcca atcttggtgg tggtatggat gaactttata agtaggcggc cgctcgagat
3301 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
3361 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
3421 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
3481 gggaggattg ggaagacaat agcaggcgtg ctggggatgc ggtgggctct atggcttctg
3541 aggcggaaag aaccagtggc ggtaatacgg ttatccacag aatcagggga taacgcagga
3601 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
3661 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
3721 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
3781 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
3841 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
3901 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
3961 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
4021 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
4081 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
4141 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
4201 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
4261 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
4321 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
4381 tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc tgccgccccg
4441 acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa
4501 gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga gtgccagccc
4561 tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt attctgtctt
4621 tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt ttcagttagc
4681 ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag
4741 ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg
4801 aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg
4861 ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga
4921 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac
4981 gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga
5041 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca
5101 cgacgagatc ctcgccgtcg ggcttgctcg ccttgagcct ggcgaacagt tcggctggcg
5161 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag
5221 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa
5281 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt
5341 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
5401 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
5461 gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
5521 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
5581 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
5641 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag
5701 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgggcctcgg
5761 cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg
5821 gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat
5881 gcttgctttg catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg
5941 actaattgag atgcttgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
6001 accctaactg acacacattc cacagctggt tctttccgcc tcaggactct tcctttttca
6061 ataaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
6121 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
6181 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
6241 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
6301 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
6361 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
6421 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
6481 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
6541 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
6601 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
6661 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
6721 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
6781 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
6841 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
6901 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
6961 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
7021 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
7081 gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
7141 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
7201 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
7261 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
7321 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
7381 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
7441 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
7501 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tacg
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pCuo DN Rac1
- 下一篇：没有了
