| 质粒名称: | pTA7001-DEST |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 71745 |
| 存储实验室: | Roger Innes |
|
载体骨架基本信息 |
|
| 载体名称: | pBI101 |
| 载体出品公司: | Clontech |
| 载体抗性: | 氯霉素和卡那霉素 |
| 空载体大小: | 9000 bp |
| 完整质粒大小: | -- |
| 载体修饰: | pBI101的T-DNA区域被一个新的T-DNA序列所取代，该序列能够使地塞米松诱导的感兴趣基因表达。pTA7001的构建在青山，T.和Chua，N.-H.（1997）一个糖皮质激素介导的转基因植物转录诱导系统中进行了描述。工厂J.11605–612。pTA7001被进一步修改，包含一个网关目的地盒式磁带插入Xho1和Spe1站点之间。 |
| 载体类型: | 植物表达 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | GAL4 |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pTA7001-DEST was a gift from Roger Innes (Addgene plasmid # 71745 ; http://n2t.net/addgene:71745 ; RRID:Addgene_71745) |
| 参考文献部分: | The KEEP ON GOING protein of Arabidopsis recruits the ENHANCED DISEASE RESISTANCE1 protein to trans-Golgi network/early endosome vesicles. Gu Y, Innes RW. Plant Physiol. 2011 Apr;155(4):1827-38. doi: 10.1104/pp.110.171785. Epub 2011 Feb 22. 10.1104/pp.110.171785 PubMed 21343429 |
买家导航
pTA7001DEST载体基本信息
pTA7001DEST载体图谱质粒图谱和多克隆位点信息
pTA7001DEST载体简介
pTA7001DEST载体序列
LOCUS pTA7001-DEST 16023 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION GATEWAY compatible derivative of plant binary vector pTA7001 from
Nam Chua's laboratory at The Rockefeller University, Dexamethasone
inducible.
ACCESSION .
VERSION .
KEYWORDS pTA7001-DEST
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 16023)
AUTHORS Gu Y, Innes RW
TITLE The KEEP ON GOING protein of Arabidopsis recruits the ENHANCED
DISEASE RESISTANCE1 protein to trans-Golgi network/early endosome
vesicles.
JOURNAL Plant Physiol. 2011 Apr;155(4):1827-38. doi: 10.1104/pp.110.171785.
Epub 2011 Feb 22.
PUBMED 21343429
REFERENCE 2 (bases 1 to 16023)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..16023
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(178..828)
/codon_start=1
/gene="tetR"
/product="tetracycline resistance regulatory protein"
/label=TetR
/translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
misc_feature 1293..1317
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
promoter 1832..2177
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind 2120..2140
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
CDS 2439..2672
/codon_start=1
/gene="UL48"
/product="transcriptional activation domain of herpes
simplex virus protein VP16 (Triezenberg et al., 1988;
Cousens et al., 1989)"
/label=VP16 AD
/translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
promoter 3964..4143
/label=NOS promoter
/note="nopaline synthase promoter"
promoter 3996..4179
/function="NOS promoter"
/label=NOS promoter
/note="nopaline synthase promoter"
terminator 5294..5546
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
promoter 5766..5812
/label=minimal CaMV 35S promoter
/note="minimal 35S promoter from cauliflower mosaic virus"
protein_bind 5846..5970
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 5995..6025
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 6079..6738
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(6145..6164)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 7080..7385
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 7264..7283
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(7426..7550)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
primer_bind complement(8092..8109)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(8092..8108)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8101..8123)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind complement(8248..8267)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8458..8479
/label=F1ori-F
/note="F1 origin, forward primer"
misc_feature 8726..8750
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS complement(10346..11494)
/codon_start=1
/product="trans-acting replication protein that binds to
and activates oriV"
/label=trfA
/translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
CDS complement(11793..12587)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
mobile_element 12779..13546
/mobile_element_type="insertion sequence:IS1"
/note="prokaryotic transposable element"
rep_origin 14169..14880
/label=oriV
/note="incP origin of replication"
CDS complement(15007..15378)
/codon_start=1
/gene="traJ"
/product="oriT-recognizing protein"
/label=traJ
/translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP"
oriT 15411..15520
/note="incP origin of transfer"
primer_bind 15743..15762
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 15996..16013
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgccagaa ggccgccaga gaggccgagc gcggccgtga ggcttggacg ctagggcagg
61 gcatgaaaaa gcccgtagcg ggctgctacg ggcgtctgac gcggtggaaa gggggagggg
121 atgttgtcta catggctctg ctgtagtgag tgggttgcgc tccggcagcg gtcctgatca
181 atcgtcaccc tttctcggtc cttcaacgtt cctgacaacg agcctccttt tcgccaatcc
241 atcgacaatc accgcgagtc cctgctcgaa cgctgcgtcc ggaccggctt cgtcgaaggc
301 gtctatcgcg gcccgcaaca gcggcgagag cggagcctgt tcaacggtgc cgccgcgctc
361 gccggcatcg ctgtcgccgg cctgctcctc aagcacggcc ccaacagtga agtagctgat
421 tgtcatcagc gcattgacgg cgtccccggc cgaaaaaccc gcctcgcaga ggaagcgaag
481 ctgcgcgtcg gccgtttcca tctgcggtgc gcccggtcgc gtgccggcat ggatgcgcgc
541 gccatcgcgg taggcgagca gcgcctgcct gaagctgcgg gcattcccga tcagaaatga
601 gcgccagtcg tcgtcggctc tcggcaccga atgcgtatga ttctccgcca gcatggcttc
661 ggccagtgcg tcgagcagcg cccgcttgtt cctgaagtgc cagtaaagcg ccggctgctg
721 aacccccaac cgttccgcca gtttgcgtgt cgtcagaccg tctacgccga cctcgttcaa
781 caggtccagg gcggcacgga tcactgtatt cggctgcaac tttgtcatgc ttgacacttt
841 atcactgata aacataatat gtccaccaac ttatcagtga taaagaatcc gcgcgttcaa
901 tcggaccagc ggaggctggt ccggaggcca gacgtgaaac ccaacatacc cctgatcgta
961 attctgagca ctgtcgcgct cgacgctgtc ggcatcggcc tgattatgcc ggtgctgccg
1021 ggcctcctgc gcgatctggt tcactcgaac gacgtcaccg cccactatgg cattctgctg
1081 gcgctgtatg cgttggtgca atttgcctgc gcacctgtgc tgggcgcgct gtcggatcgt
1141 ttcgggcggc ggccaatctt gctcgtctcg ctggccggcg ccagatctgg ggaaccctgt
1201 ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc
1261 cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac
1321 actgatagtt tcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
1381 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
1441 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
1501 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
1561 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
1621 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
1681 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
1741 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
1801 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
1861 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
1921 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
1981 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
2041 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
2101 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
2161 ttcatttgga gagaacacgg gggactctag tttaaacaat ggcagatcca atgaagctac
2221 tgtcttctat cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag
2281 aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa
2341 ccaaaaggtc tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac
2401 tggaacagct atttctactg atttttccta ggtcgagcgc ccccccgacc gatgtcagcc
2461 tgggggacga gctccactta gacggcgagg acgtggcgat ggcgcatgcc gacgcgctag
2521 acgatttcga tctggacatg ttgggggacg gggattcccc gggtccggga tttacccccc
2581 acgactccgc cccctacggc gctctggata tggccgactt cgagtttgag cagatgttta
2641 ccgatgccct tggaattgac gagtacggtg gggatccaat tcagcaagcc actgcaggag
2701 tctcacaaga cacttcggaa aatcctaaca aaacaatagt tcctgctgca ttaccacagc
2761 tcacccctac cttggtgtca ctgctggagg tgattgaacc cgaggtgttg tatgcaggat
2821 atgatagctc tgttccagat tcagcatgga gaattatgac cacactcaac atgttaggtg
2881 ggcgtcaagt gattgcagca gtgaaatggg caaaggcgat accaggcttc agaaacttac
2941 acctggatga ccaaatgacc ctgctacagt actcatggat gtttctcatg gcatttgccc
3001 tgggttggag atcatacaga caatcaagtg gaaacctgct ctgctttgct cctgatctga
3061 ttattaatga gcagagaatg tctctaccct gcatgtatga ccaatgtaaa cacatgctgt
3121 ttgtctcctc tgaattacaa agattgcagg tatcctatga agagtatctc tgtatgaaaa
3181 ccttactgct tctctcctca gttcctaagg aaggtctgaa gagccaagag ttatttgatg
3241 agattcgaat gacttatatc aaagagctag gaaaagccat cgtcaaaagg gaagggaact
3301 ccagtcagaa ctggcaacgg ttttaccaac tgacaaagct tctggactcc atgcatgagg
3361 tggttgagaa tctccttacc tactgcttcc agacattttt ggataagacc atgagtattg
3421 aattcccaga gatgttagct gaaatcatca ctaatcagat accaaaatat tcaaatggaa
3481 atatcaaaaa gcttctgttt catcaaaaat gactcgacct aactgagtaa gctagcttgt
3541 tcgagtatta tggcattggg aaaactgttt ttcttgtacc atttgttgtg cttgtaattt
3601 actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga tggagaagag
3661 ttaatgaatg atatggtcct tttgttcatt ctcaaattaa tattatttgt tttttctctt
3721 atttgttgtg tgttgaattt gaaattataa gagatatgca aacattttgt tttgagtaaa
3781 aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat gaggagtaaa acactagatc
3841 cccaaacaag cttgaaactg aaggcgggaa acgacaatct gatcatgagc ggagaattaa
3901 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
3961 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
4021 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
4081 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
4141 ttagagtctc atattcactc tcaatccaaa taatctgcac cggatccgct agaggatctc
4201 gacctgcaag atcccggggg gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct
4261 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
4321 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
4381 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
4441 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
4501 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
4561 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
4621 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
4681 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
4741 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
4801 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
4861 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
4921 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
4981 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
5041 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
5101 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
5161 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
5221 cgacgcccca gcactcgtcc gggatcttgg aggtgatgta acatgatcac aagctgatcc
5281 cccgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
5341 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
5401 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
5461 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
5521 cgcggtgtca tctatgttac tagatcggga attgatcccc cctcgacagc ttgcatgccg
5581 gtcgactcta gaggatccgg gtgacagccc tccgacgggt gacagccctc cgacgggtga
5641 cagccctccg aattctagag gatccgggtg acagccctcc gacgggtgac agccctccga
5701 cgggtgacag ccctccgaat tcgagctcgg tacccgggga tctgtcgacc tcgatcgaga
5761 tcttcgcaag acccttcctc tatataagga agttcatttc atttggagag gacacgctga
5821 agctagtcga ctctagcctc gaatcacaag tttgtacaaa aaagctgaac gagaaacgta
5881 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
5941 ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca
6001 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca
6061 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
6121 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
6181 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
6241 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
6301 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
6361 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
6421 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
6481 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
6541 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
6601 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
6661 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
6721 tggcagggcg gggcgtaaac gcgtggatcc ggcttactaa aagccagata acagtatgcg
6781 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
6841 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
6901 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
6961 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
7021 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa
7081 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
7141 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
7201 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
7261 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg
7321 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
7381 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata
7441 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
7501 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt gattcgaggc
7561 tagaggatcg actagtcgac tagtcgatcc aggcctccca gctttcgtcc gtatcatcgg
7621 tttcgacaac gttcgtcaag ttcaatgcat cagtttcatt gcccacacac cagaatccta
7681 ctaagtttga gtattatggc attggaaaag ctgttttctt ctatcatttg ttctgcttgt
7741 aatttactgt gttctttcag tttttgtttt cggacatcaa aatgcaaatg gatggataag
7801 agttaataaa tgatatggtc cttttgttca ttctcaaatt attattatct gttgttttta
7861 ctttaatggg ttgaatttaa gtaagaaagg aactaacagt gtgatattaa ggtgcaatgt
7921 tagacatata aaacagtctt tcacctctct ttggttatgt cttgaattgg tttgtttctt
7981 cacttatctg tgtaatcaag tttactatga gtctatgatc aagtaattat gcaatcaagt
8041 taagtacagt ataggctttt tgtgtcgagg gggtaccgag tcgaggaatt cactggccgt
8101 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
8161 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
8221 acagttgcgc agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
8281 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
8341 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
8401 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
8461 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
8521 aagggatttt gccgatttcg gaaccaccat caaacaggat tttcgcctgc tggggcaaac
8581 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
8641 gcccgtctca ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc cgcaatgtgt
8701 tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc
8761 aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag
8821 tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga
8881 tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag
8941 ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa tatcatctcc
9001 ctcgcagaga tccgaattat cagccttctt attcatttct cgcttaaccg tgacaggctg
9061 tcgatcttga gaactatgcc gacataatag gaaatcgctg gataaagccg ctgaggaagc
9121 tgagtggcgc tatttcttta gaagtgaacg ttgacgatat caactcccct atccattgct
9181 caccgaatgg tacaggtcgg ggacccgaag ttccgactgt cggcctgatg catccccggc
9241 tgatcgaccc cagatctggg gctgagaaag cccagtaagg aaacaactgt aggttcgagt
9301 cgcgagatcc cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac
9361 gccaggccga gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct
9421 actggaacga gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc
9481 acgggaggtt gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc
9541 aggcccgctg cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc
9601 acgcccgcag ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc
9661 agagcggcag agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc
9721 ccgcccggca ggcggtagac cgaaataaac aacaagctcc agaatagcga aatattaagt
9781 gcgccgagga tgaagatgcg catccaccag attcccgttg gaatctgtcg gacgatcatc
9841 acgagcaata aacccgccgg caacgcccgc agcagcatac cggcgacccc tcggcctcgc
9901 tgttcgggct ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt ggggccgtcc
9961 tcctgtttga agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc gaatgccacg
10021 gcatctcgca accgttcagc gaacgcctcc atgggctttt tctcctcgtg ctcgtaaacg
10081 gacccgaaca tctctggagc tttcttcagg gccgacaatc ggatctcgcg gaaatcctgc
10141 acgtcggccg ctccaagccg tcgaatctga gccttaatca caattgtcaa ttttaatcct
10201 ctgtttatcg gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag
10261 tgcgtcgagc agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc
10321 cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc
10381 aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc
10441 gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga
10501 gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca
10561 gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga
10621 tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga
10681 agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg
10741 ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg
10801 accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg
10861 tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct
10921 cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca
10981 gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt
11041 ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt
11101 ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt
11161 ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga
11221 tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg
11281 cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg
11341 ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga
11401 cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt
11461 gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga
11521 ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca
11581 gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt
11641 acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc
11701 cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct
11761 tgtcgccggc atcgttgcgc cacatctagg tactaaaaca attcatccag taaaatataa
11821 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
11881 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
11941 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
12001 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
12061 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
12121 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
12181 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
12241 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
12301 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
12361 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
12421 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
12481 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
12541 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
12601 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
12661 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
12721 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca caattatggg
12781 tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt
12841 ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa
12901 gcaccgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg caactgcagt
12961 tcacttacac cgcttctcaa cccggtacgc accagaaaat cattgatatg gccatgaatg
13021 gcgttggatg ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg attttacgtc
13081 acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca gtgacgtcat
13141 cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc gccagcgctg
13201 gctgttttac gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat tcggtgaacg
13261 cactatggcg acgctggggc gtcttatgag cctgctgtca ccctttgacg tggtgatatg
13321 gatgacggat ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag
13381 caagcgatat acgcagcgaa ttgagcggca taacctgaat ctgaggcagc acctggcacg
13441 gctgggacgg aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg
13501 gcattatctg aacataaaac actatcaata agttggagtc attacccaat tatgatagaa
13561 tttacaagct ataaggttat tgtcctgggt ttcaagcatt agtccatgca agtttttatg
13621 ctttgcccat tctatagata tattgataag cgcgctgcct atgccttgcc ccctgaaatc
13681 cttacatacg gcgatatctt ctatataaaa gatatattat cttatcagta ttgtcaatat
13741 attcaaggca atctgcctcc tcatcctctt catcctcttc gtcttggtag ctttttaaat
13801 atggcgcttc atagagtaat tctgtaaagg tccaattctc gttttcatac ctcggtataa
13861 tcttacctat cacctcaaat ggttcgctgg gtttatcgca cccccgaaca cgagcacggc
13921 acccgcgacc actatgccaa gaatgcccaa ggtaaaaatt gccggccccg ccatgaagtc
13981 cgtgaatgcc ccgacggccg aagtgaaggg caggccgcca cccaggccgc cgccctcact
14041 gcccggcacc tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa tgcttccggg
14101 cgtcgcgctc gggctgatcg cccatcccgt tactgccccg atcccggcaa tggcaaggac
14161 tgccagcgct gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg
14221 ggatgggagg cccgcgttag cgggccggga gggttcgaga agggggggca ccccccttcg
14281 gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg
14341 gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc
14401 aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc
14461 tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc
14521 ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga
14581 aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg
14641 gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag
14701 tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac
14761 gccggcggcc gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc
14821 catagacggc cgccagccca gcggcgaggg caaccagccc ggtgagcgtc ggaaaggcgc
14881 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
14941 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
15001 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
15061 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
15121 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
15181 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
15241 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
15301 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
15361 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
15421 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
15481 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
15541 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
15601 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
15661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
15721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
15781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
15841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
15901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
15961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
16021 aag
//
