出品公司: | Invitrogen |
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载体名称: | pPink-HC |
质粒类型: | 毕赤酵母(Pink酵母)蛋白表达载体 |
表达水平: | 低拷贝 |
启动子: | AOX1 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 7667 bp |
5' 测序引物及序列: | 5´ AOX1:5´-GACTGGTTCCAATTGACAAGC-3´; |
3' 测序引物及序列: | CYC1-R: GCGTGAATGTAAGCGTGAC |
载体标签: | / |
载体抗性: | 氨苄 |
筛选标记: | ADE2 |
备注: |
细胞内蛋白表达载体
|
产品目录号: | A11150 |
稳定性: | 稳定 Stable |
组成型: | 组成型 Constitutive |
病毒/非病毒: | 非病毒 |
买家导航
pPink-HC载体质粒基本信息
pPink-HC质粒图谱载体图谱和pPink-HC载体序列质粒序列多克隆位点信息
pPink-HC质粒载体简介
pPink-HC质粒序列载体序列
LOCUS pPink-HC 7667 bp ds-DNA circular SYN 04-二月-2013 DEFINITION Pichia pastoris integrating vector for high-copy expression in the PichiaPink(TM) system. ACCESSION . VERSION . KEYWORDS pPink-HC SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT VNTAUTHORNAME|biofeng.com| COMMENT Linearize within PpTRP2 for integration. FEATURES Location/Qualifiers source 1..7667 /organism="synthetic DNA construct" /lab_host="Pichia pastoris" /mol_type="other DNA" promoter 2..940 /gene="Pichia pastoris AOX1" /note="AOX1 promoter" /note="inducible promoter, regulated by methanol" /note="color: #ffffff; direction: RIGHT" misc_feature 941..991 /note="MCS" /note="multiple cloning site" /note="color: #99ccff" terminator 1017..1266 /gene="S. cerevisiae CYC1" /note="CYC1 terminator" /note="transcription terminator for CYC1" /note="color: #ffffff" promoter 1286..1298 /gene="Pichia pastoris ADE2" /note="PpADE2 HC promoter" /note="weak 13-bp promoter fragment that requires high-copy integration for efficient expression" /note="color: #ffffff; direction: RIGHT" CDS 1299..2990 /codon_start=1 /gene="Pichia pastoris ADE2" /product="phosphoribosylaminoimidazole carboxylase from Pichia pastoris" /note="PpADE2" /note="essential for the biosynthesis of purine nucleotides, including adenine" /note="color: #ff7f50" promoter 3217..3366 /gene="Pichia pastoris TRP2" /note="PpTRP2 promoter" /note="promoter for Pichia pastoris TRP2" /note="color: #ffffff; direction: RIGHT" CDS 3367..4983 /codon_start=1 /gene="Pichia pastoris TRP2" /product="anthranilate synthase from Pichia pastoris" /note="PpTRP2" /note="catalyzes the initial step of tryptophan biosynthesis" /note="color: #ff7f50" rep_origin complement(5447..6035) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(6206..7066) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: ???1:?6206?..?6997?/?#ccffcc ???2:?6998?..?7066?/?#ccffcc?/?signal?sequence Cleavage site after base 6997" promoter complement(7067..7171) /gene="bla" /note="AmpR promoter" /note="color: #ffffff; direction: LEFT" ORIGIN 1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag 61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt 121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc 181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta 241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta 301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg 361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct 421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg 481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt 541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct 601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct 661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact 721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat 781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt 841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga 901 caacttgaga agatcaaaaa acaactaatt attcgaaacg gaattccgga ccggcatgcc 961 aaggcctcag gtaccggccg gccatttaaa tacaggcccc ttttcctttg tcgatatcat 1021 gtaattagtt atgtcacgct tacattcacg ccctcctccc acatccgctc taaccgaaaa 1081 ggaaggagtt agacaacctg aagtctaggt ccctatttat tttttttaat agttatgtta 1141 gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacaa acgcgtgtac 1201 gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaaggcttta 1261 atttgcaagc tggatccgcg gccgccttcc aaactctcat ggattctcag gtaataggta 1321 ttctaggagg aggccagcta ggccgaatga ttgttgaggc cgctagcagg ctcaatatca 1381 agaccgtgat tcttgatgat ggtttttcac ctgctaagca cattaatgct gcgcaagacc 1441 acatcgacgg atcattcaaa gatgaggagg ctatcgccaa gttagctgcc aaatgtgatg 1501 ttctcactgt agagattgag catgtcaaca cagatgctct aaagagagtt caagacagaa 1561 ctggaatcaa gatatatcct ttaccagaga caatcgaact aatcaaggat aagtacttgc 1621 aaaaggaaca tttgatcaag cacaacattt cggtgacaaa gtctcagggt atagaatcta 1681 atgaaaaggc gctgcttttg tttggagaag agaatggatt tccatatctg ttgaagtccc 1741 ggactatggc ttatgatgga agaggcaatt ttgtagtgga gtctaaagag gacatcagta 1801 aggcattaga gttcttgaaa gatcgtccat tgtatgccga gaagtttgct ccttttgtta 1861 aagaattagc ggtaatggtt gtgagatcac tggaaggcga agtattctcc tacccaaccg 1921 tagaaactgt gcacaaggac aatatctgtc atattgtgta tgctccggcc agagttaatg 1981 acaccatcca aaagaaagct caaatattag ctgaaaacac tgtgaagact ttcccaggcg 2041 ctggaatctt cggagttgag atgttcctat tgtctgatgg agaacttctt gtaaatgaga 2101 ttgctccaag gccccacaat tctggtcact atacaatcga tgcatgtgta acatctcagt 2161 tcgaagcaca tgtaagagcc ataactggtc tgccaatgcc actagatttc accaaactat 2221 ctacttccaa caccaacgct attatgctca atgttttggg tgctgaaaaa tctcacgggg 2281 aattagagtt ttgtagaaga gccttagaaa cacccggtgc ttctgtatat ctgtacggaa 2341 agaccacccg attggctcgt aagatgggtc atatcaacat aataggatct tccatgttgg 2401 aagcagaaca aaagttagag tacattctag aagaatcaac ccacttacca tccagtactg 2461 tatcagctga cactaaaccg ttggttggag ttatcatggg ttcagactct gatctacctg 2521 tgatttcgaa aggttgcgat attttaaaac agtttggtgt tccattcgaa gttactattg 2581 tctctgctca tagaacacca cagagaatga ccagatatgc ctttgaagcc gctagtagag 2641 gtatcaaggc tatcattgca ggtgctggtg gtgctgctca tcttccagga atggttgctg 2701 ccatgactcc gttgccagtc attggtgttc ctgtcaaggg ctctacgttg gatggtgtag 2761 actcgctaca ctcgattgtc caaatgccta gaggtgttcc tgtggctacg gttgctatca 2821 acaacgccac caatgccgct ctgttggcca tcaggatttt aggtacaatt gaccacaaat 2881 ggcaaaagga aatgtccaag tatatgaatg caatggagac cgaagtgttg gggaaggcat 2941 ccaacttgga atctgaaggg tatgaatcct atttgaagaa tcgtctttga atttagtatt 3001 gttttttaat agatgtatat ataatagtac acgtaactta tctattccat tcataatttt 3061 attttaaagg ttcggtagaa atttgtcctc caaaaagttg gttagagcct ggcagttttg 3121 ataggcatta ttatagattg ggtaatattt accctgcacc tggaggaact ttgcaaagag 3181 cctcatgtgc ggcgcgccag gccataatgg ccaaacggtt tctcaattac tatatactac 3241 taaccattta cctgtagcgt atttcttttc cctcttcgcg aaagctcaag ggcatcttct 3301 tgactcatga aaaatatctg gatttcttct gacagatcat cacccttgag cccaactctc 3361 tagcctatga gtgtaagtga tagtcatctt gcaacagatt attttggaac gcaactaaca 3421 aagcagatac acccttcagc agaatccttt ctggatattg tgaagaatga tcgccaaagt 3481 cacagtcctg agacagttcc taatctttac cccatttaca agttcatcca atcagacttc 3541 ttaacgcctc atctggctta tatcaagctt accaacagtt cagaaactcc cagtccaagt 3601 ttcttgcttg aaagtgcgaa gaatggtgac accgttgaca ggtacacctt tatgggacat 3661 tcccccagaa aaataatcaa gactgggcct ttagagggtg ctgaagttga ccccttggtg 3721 cttctggaaa aagaactgaa gggcaccaga caagcgcaac ttcctggtat tcctcgtcta 3781 agtggtggtg ccataggata catctcgtac gattgtatta agtactttga accaaaaact 3841 gaaagaaaac tgaaagatgt tttgcaactt ccggaagcag ctttgatgtt gttcgacacg 3901 atcgtggctt ttgacaatgt ttatcaaaga ttccaggtaa ttggaaacgt ttctctatcc 3961 gttgatgact cggacgaagc tattcttgag aaatattata agacaagaga agaagtggaa 4021 aagatcagta aagtggtatt tgacaataaa actgttccct actatgaaca gaaagatatt 4081 attcaaggcc aaacgttcac ctctaatatt ggtcaggaag ggtatgaaaa ccatgttcgc 4141 aagctgaaag aacatattct gaaaggagac atcttccaag ctgttccctc tcaaagggta 4201 gccaggccga cctcattgca ccctttcaac atctatcgtc atttgagaac tgtcaatcct 4261 tctccataca tgttctatat tgactatcta gacttccaag ttgttggtgc ttcacctgaa 4321 ttactagtta aatccgacaa caacaacaaa atcatcacac atcctattgc tggaactctt 4381 cccagaggta aaactatcga agaggacgac aattatgcta agcaattgaa gtcgtctttg 4441 aaagacaggg ccgagcacgt catgctggta gatttggcca gaaatgatat taaccgtgtg 4501 tgtgagccca ccagtaccac ggttgatcgt ttattgactg tggagagatt ttctcatgtg 4561 atgcatcttg tgtcagaagt cagtggaaca ttgagaccaa acaagactcg cttcgatgct 4621 ttcagatcca ttttcccagc aggaaccgtc tccggtgctc cgaaggtaag agcaatgcaa 4681 ctcataggag aattggaagg agaaaagaga ggtgtttatg cgggggccgt aggacactgg 4741 tcgtacgatg gaaaatcgat ggacacatgt attgccttaa gaacaatggt cgtcaaggac 4801 ggtgtcgctt accttcaagc cggaggtgga attgtctacg attctgaccc ctatgacgag 4861 tacatcgaaa ccatgaacaa aatgagatcc aacaataaca ccatcttgga ggctgagaaa 4921 atctggaccg ataggttggc cagagacgag aatcaaagtg aatccgaaga aaacgatcaa 4981 tgaacggagg acgtaagtag gaatttatgg tttggccata atggcctagc ttggcgtaat 5041 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 5101 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 5161 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 5221 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 5281 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 5341 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 5401 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 5461 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 5521 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 5581 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 5641 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 5701 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 5761 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 5821 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 5881 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 5941 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 6001 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 6061 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 6121 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 6181 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 6241 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 6301 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 6361 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 6421 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 6481 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 6541 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 6601 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 6661 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 6721 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 6781 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 6841 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 6901 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 6961 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 7021 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 7081 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 7141 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 7201 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 7261 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 7321 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 7381 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga 7441 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 7501 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt 7561 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc 7621 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattg //