质粒名称: | pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro |
---|---|
出品公司: | Addgene |
目录编号: | 141371 |
存储实验室: | Nevan Krogan |
载体骨架基本信息 |
|
载体名称: | pLVX-EF1alpha-IRES-Puro |
载体出品公司: | Takara Bio |
空载体大小: | 8825 bp |
完整质粒大小: | 8825 bp |
载体类型: | 哺乳细胞表达, Lentiviral |
筛选标记: | Puromycin |
载体抗性: | 氨苄霉素 |
生长温度: | 30 ℃ |
宿主菌: | NEB Stable |
拷贝数: | 未知 |
插入基因信息 |
|
插入基因名称: | SARS-CoV-2-nsp5-C145A / 3CLPro |
物种来源: | SARS-CoV-2 |
基因突变: | C145A, human codon optimized |
启动子: | EF1alpha |
标签/融合蛋白: | 2xStrep (C terminal on insert) |
克隆方法: | 未知 |
文献引用方法 |
|
材料和方法部分: | pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro was a gift from Nevan Krogan (Addgene plasmid # 141371 ; http://n2t.net/addgene:141371 ; RRID:Addgene_141371) |
参考文献部分: | A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, Shoichet BK, Krogan NJ. Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. Epub 2020 Apr 30. 10.1038/s41586-020-2286-9 PubMed 32353859 |
pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro 9834 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus. ACCESSION . VERSION . KEYWORDS pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9834) AUTHORS Gordon DE, et al. TITLE A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug Repurposing JOURNAL BioRxiv March 2020 REFERENCE 2 (bases 1 to 9834) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..9834 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2028..2143 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" promoter 2338..3519 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2568..3510 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3467..3487 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" CDS 4489..4512 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" CDS 4549..4572 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" misc_feature 4584..5157 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(4751..4768) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 4978..4997 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5190..5789 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5190..5209) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 5686..5706 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 5803..6391 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5856..5876) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6274..6285) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6598..7231 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7359..7375) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7359..7375) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7372..7394) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7383..7399 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7407..7437) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7452..7473 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7590..7607) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7761..8349) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7841..7860) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8520..9380) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9143..9162 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9381..9485) /gene="bla" /label=AmpR promoter polyA_signal 9533..9667 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9570..9589) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9624..9643 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc 3541 gccgccacca tgtctggttt taggaaaatg gcgttcccca gcggtaaagt tgaaggatgt 3601 atggtccaag taacctgtgg taccactacc cttaatgggc tttggttgga cgacgtagtc 3661 tactgccccc gacacgtaat ctgcactagt gaggatatgc ttaatcccaa ttacgaagac 3721 cttttgattc ggaaatccaa tcacaacttc ctggtccaag cgggcaacgt ccaactcagg 3781 gttattggac atagtatgca gaattgcgta ctgaagctca aagtcgatac tgcaaacccc 3841 aagacgccca agtataaatt cgtccgaatc caaccaggcc aaacattttc cgtattggct 3901 tgctataatg gaagccccag cggtgtctac caatgtgcaa tgagaccaaa ctttacgata 3961 aagggttcat ttctgaacgg ctctgccggt tccgttggtt ttaacatcga ctatgactgt 4021 gtatcctttt gctacatgca ccatatggaa ctccctaccg gtgtccacgc cggtacagat 4081 ctggaaggaa atttctacgg tccgttcgtt gaccggcaaa ccgcgcaagc ggctggaacc 4141 gacacaacga ttacagtgaa tgtgctcgcg tggctgtacg cagcagtcat aaacggagac 4201 aggtggtttc tgaaccgatt tacgacgact ctcaatgact tcaaccttgt tgcgatgaag 4261 tacaattacg agccactcac ccaggaccat gttgatatcc tgggtcccct cagtgcccag 4321 acagggatcg cagttctcga tatgtgcgcg tcactgaagg agcttctcca aaatggaatg 4381 aatgggcgga ccatacttgg ttccgcactc ctcgaagatg aatttactcc atttgacgtg 4441 gtcagacaat gcagtggggt cactttccag ctcgaaggcg gcgggggatg gagccatcca 4501 caattcgaga aaggcggtgg ttcaggagga ggtagcgggg gtggatcatg gtcacatccg 4561 cagtttgaaa agtaaggatc ccgcccctct ccctcccccc cccctaacgt tactggccga 4621 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg 4681 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg 4741 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt 4801 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac 4861 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca 4921 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg 4981 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg 5041 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa 5101 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct 5161 tgccacaacc cacaaggaga cgaccttcca tgaccgagta caagcccacg gtgcgcctcg 5221 ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc 5281 ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag 5341 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg 5401 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga 5461 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag 5521 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct 5581 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg 5641 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct 5701 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct 5761 ggtgcatgac ccgcaagccc ggtgcctaga cgcgtctgga acaatcaacc tctggattac 5821 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 5881 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 5941 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 6001 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 6061 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 6121 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 6181 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg 6241 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 6301 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg 6361 agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag 6421 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc 6481 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac 6541 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg 6601 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac 6661 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga 6721 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata 6781 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga 6841 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc 6901 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg 6961 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct 7021 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc 7081 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 7141 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 7201 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag 7261 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt 7321 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga 7381 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 7441 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 7501 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 7561 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 7621 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 7681 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 7741 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 7801 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 7861 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 7921 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 7981 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 8041 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 8101 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 8161 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 8221 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 8281 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 8341 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 8401 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 8461 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 8521 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 8581 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 8641 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 8701 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 8761 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 8821 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 8881 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 8941 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 9001 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 9061 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 9121 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 9181 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 9241 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 9301 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 9361 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 9421 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 9481 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt 9541 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 9601 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 9661 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc 9721 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt 9781 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt //
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇:pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-Puro
- 下一篇:没有了