| 质粒名称: | pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G) |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 103870 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 空载体大小: | 5428 bp |
| 完整质粒大小: | 9846bp |
| 载体类型: | 哺乳细胞、CRISPR表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称1: | PspCas13b |
| 物种来源: | Prevotella sp. P5-125 |
| 插入大小: | 3270 bp |
| 基因突变: | Changed histidne 133 to alanine and histidine 1058 to alanine to remove RNAse activity. |
| GenBank编号 | WP_044065294 |
| 插入基因名称2: | huADAR2DD / human ADAR2 deaminase domain |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1158 |
| 基因突变: | Only the deaminase domain (AA 276-702 are expressed). Glutamate 488 is mutated to glutamine. Threonine 375 is mutated to glycine to enhance specificity of deaminase reaction. |
| 标签/融合蛋白: | GSGGGGS (N terminal on insert) |
|
文献引用方法 |
|
| 材料和方法部分: | pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G) was a gift from Feng Zhang (Addgene plasmid # 103870 ; http://n2t.net/addgene:103870 ; RRID:Addgene_103870) |
| 参考文献部分: | RNA editing with CRISPR-Cas13. Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F. Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180. 10.1126/science.aaq0180 PubMed 29070703 |
pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G) 9864 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G
mutation in the ADAR deaminase domain confers increased specificity
with slightly reduced activity..
ACCESSION .
VERSION .
KEYWORDS pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9864)
AUTHORS Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung
J, Zhang F
TITLE RNA editing with CRISPR-Cas13.
JOURNAL Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
PUBMED 29070703
REFERENCE 2 (bases 1 to 9864)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9864
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 9..30
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(149..169)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 154..483
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 334..469
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 396..415
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 550..1344
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(604..623)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 1214..1233
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 1518..1639
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1555..1574)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1609..1628
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(1688..1704)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1688..1704)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(1701..1723)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 1712..1728
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1736..1766)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1781..1802
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(1919..1936)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2090..2678)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2170..2189)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2849..3709)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3472..3491
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3710..3814)
/gene="bla"
/label=AmpR promoter
primer_bind complement(3889..3908)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 4080..4459
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 4460..4663
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 4614..4634
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 4708..4727
/label=T7
/note="T7 promoter, forward primer"
promoter 4708..4726
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 8050..8076
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
primer_bind complement(9300..9317)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 9306..9530
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 9576..140
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9663..9682)
/label=F1ori-R
/note="F1 origin, reverse primer"
ORIGIN
1 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
61 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
121 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
181 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
241 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
301 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
361 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
421 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
481 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
541 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
601 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
661 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
721 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
781 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
841 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
901 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
961 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
1021 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
1081 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
1141 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
1201 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
1261 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
1321 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
1381 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
1441 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
1501 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
1561 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
1621 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
1681 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
1741 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
1801 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
1861 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
1921 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
1981 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
2041 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
2101 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
2161 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
2221 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
2281 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
2341 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
2401 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
2461 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
2521 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
2581 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
2641 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
2701 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
2761 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
2821 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
2881 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
2941 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
3001 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
3061 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
3121 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
3181 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
3241 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
3301 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
3361 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
3421 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
3481 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
3541 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
3601 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
3661 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
3721 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
3781 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
3841 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
3901 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
3961 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
4021 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
4081 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
4141 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
4201 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
4261 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
4321 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
4381 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
4441 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
4501 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
4561 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
4621 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
4681 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
4741 ctagcgttta aacttaagct tgccaccatg aacatccccg ctctggtgga aaaccagaag
4801 aagtactttg gcacctacag cgtgatggcc atgctgaacg ctcagaccgt gctggaccac
4861 atccagaagg tggccgatat tgagggcgag cagaacgaga acaacgagaa tctgtggttt
4921 caccccgtga tgagccacct gtacaacgcc aagaacggct acgacaagca gcccgagaaa
4981 accatgttca tcatcgagcg gctgcagagc tacttcccat tcctgaagat catggccgag
5041 aaccagagag agtacagcaa cggcaagtac aagcagaacc gcgtggaagt gaacagcaac
5101 gacatcttcg aggtgctgaa gcgcgccttc ggcgtgctga agatgtacag ggacctgacc
5161 aacgcataca agacctacga ggaaaagctg aacgacggct gcgagttcct gaccagcaca
5221 gagcaacctc tgagcggcat gatcaacaac tactacacag tggccctgcg gaacatgaac
5281 gagagatacg gctacaagac agaggacctg gccttcatcc aggacaagcg gttcaagttc
5341 gtgaaggacg cctacggcaa gaaaaagtcc caagtgaata ccggattctt cctgagcctg
5401 caggactaca acggcgacac acagaagaag ctgcacctga gcggagtggg aatcgccctg
5461 ctgatctgcc tgttcctgga caagcagtac atcaacatct ttctgagcag gctgcccatc
5521 ttctccagct acaatgccca gagcgaggaa cggcggatca tcatcagatc cttcggcatc
5581 aacagcatca agctgcccaa ggaccggatc cacagcgaga agtccaacaa gagcgtggcc
5641 atggatatgc tcaacgaagt gaagcggtgc cccgacgagc tgttcacaac actgtctgcc
5701 gagaagcagt cccggttcag aatcatcagc gacgaccaca atgaagtgct gatgaagcgg
5761 agcagcgaca gattcgtgcc tctgctgctg cagtatatcg attacggcaa gctgttcgac
5821 cacatcaggt tccacgtgaa catgggcaag ctgagatacc tgctgaaggc cgacaagacc
5881 tgcatcgacg gccagaccag agtcagagtg atcgagcagc ccctgaacgg cttcggcaga
5941 ctggaagagg ccgagacaat gcggaagcaa gagaacggca ccttcggcaa cagcggcatc
6001 cggatcagag acttcgagaa catgaagcgg gacgacgcca atcctgccaa ctatccctac
6061 atcgtggaca cctacacaca ctacatcctg gaaaacaaca aggtcgagat gtttatcaac
6121 gacaaagagg acagcgcccc actgctgccc gtgatcgagg atgatagata cgtggtcaag
6181 acaatcccca gctgccggat gagcaccctg gaaattccag ccatggcctt ccacatgttt
6241 ctgttcggca gcaagaaaac cgagaagctg atcgtggacg tgcacaaccg gtacaagaga
6301 ctgttccagg ccatgcagaa agaagaagtg accgccgaga atatcgccag cttcggaatc
6361 gccgagagcg acctgcctca gaagatcctg gatctgatca gcggcaatgc ccacggcaag
6421 gatgtggacg ccttcatcag actgaccgtg gacgacatgc tgaccgacac cgagcggaga
6481 atcaagagat tcaaggacga ccggaagtcc attcggagcg ccgacaacaa gatgggaaag
6541 agaggcttca agcagatctc cacaggcaag ctggccgact tcctggccaa ggacatcgtg
6601 ctgtttcagc ccagcgtgaa cgatggcgag aacaagatca ccggcctgaa ctaccggatc
6661 atgcagagcg ccattgccgt gtacgatagc ggcgacgatt acgaggccaa gcagcagttc
6721 aagctgatgt tcgagaaggc ccggctgatc ggcaagggca caacagagcc tcatccattt
6781 ctgtacaagg tgttcgcccg cagcatcccc gccaatgccg tcgagttcta cgagcgctac
6841 ctgatcgagc ggaagttcta cctgaccggc ctgtccaacg agatcaagaa aggcaacaga
6901 gtggatgtgc ccttcatccg gcgggaccag aacaagtgga aaacacccgc catgaagacc
6961 ctgggcagaa tctacagcga ggatctgccc gtggaactgc ccagacagat gttcgacaat
7021 gagatcaagt cccacctgaa gtccctgcca cagatggaag gcatcgactt caacaatgcc
7081 aacgtgacct atctgatcgc cgagtacatg aagagagtgc tggacgacga cttccagacc
7141 ttctaccagt ggaaccgcaa ctaccggtac atggacatgc ttaagggcga gtacgacaga
7201 aagggctccc tgcagcactg cttcaccagc gtggaagaga gagaaggcct ctggaaagag
7261 cgggcctcca gaacagagcg gtacagaaag caggccagca acaagatccg cagcaaccgg
7321 cagatgagaa acgccagcag cgaagagatc gagacaatcc tggataagcg gctgagcaac
7381 agccggaacg agtaccagaa aagcgagaaa gtgatccggc gctacagagt gcaggatgcc
7441 ctgctgtttc tgctggccaa aaagaccctg accgaactgg ccgatttcga cggcgagagg
7501 ttcaaactga aagaaatcat gcccgacgcc gagaagggaa tcctgagcga gatcatgccc
7561 atgagcttca ccttcgagaa aggcggcaag aagtacacca tcaccagcga gggcatgaag
7621 ctgaagaact acggcgactt ctttgtgctg gctagcgaca agaggatcgg caacctgctg
7681 gaactcgtgg gcagcgacat cgtgtccaaa gaggatatca tggaagagtt caacaaatac
7741 gaccagtgca ggcccgagat cagctccatc gtgttcaacc tggaaaagtg ggccttcgac
7801 acataccccg agctgtctgc cagagtggac cgggaagaga aggtggactt caagagcatc
7861 ctgaaaatcc tgctgaacaa caagaacatc aacaaagagc agagcgacat cctgcggaag
7921 atccggaacg ccttcgatgc aaacaattac cccgacaaag gcgtggtgga aatcaaggcc
7981 ctgcctgaga tcgccatgag catcaagaag gcctttgggg agtacgccat catgaaggga
8041 agcctgcagc tgcctccact tgaaagactg acactgggat ccggaggagg tggaagccag
8101 ctgcatttac cgcaggtttt agctgacgct gtctcacgcc tggtcctggg taagtttggt
8161 gacctgaccg acaacttctc ctcccctcac gctcgcagaa aagtgctggc tggagtcgtc
8221 atgacaacag gcacagatgt taaagatgcc aaggtgataa gtgtttctac aggaggcaaa
8281 tgtattaatg gtgaatacat gagtgatcgt ggccttgcat taaatgactg ccatgcagaa
8341 ataatatctc ggagatcctt gctcagattt ctttatacac aacttgagct ttacttaaat
8401 aacaaagatg atcaaaaaag atccatcttt cagaaatcag agcgaggggg gtttaggctg
8461 aaggagaatg tccagtttca tctgtacatc agcacctctc cctgtggaga tgccagaatc
8521 ttctcaccac atgagccaat cctggaagaa ccagcagata gacacccaaa tcgtaaagca
8581 agaggacagc tacggaccaa aatagagtct ggtcagggga cgattccagt gcgctccaat
8641 gcgagcatcc aaacgtggga cggggtgctg caaggggagc ggctgctcac catgtcctgc
8701 agtgacaaga ttgcacgctg gaacgtggtg ggcatccagg gatcactgct cagcattttc
8761 gtggagccca tttacttctc gagcatcatc ctgggcagcc tttaccacgg ggaccacctt
8821 tccagggcca tgtaccagcg gatctccaac atagaggacc tgccacctct ctacaccctc
8881 aacaagcctt tgctcagtgg catcagcaat gcagaagcac ggcagccagg gaaggccccc
8941 aacttcagtg tcaactggac ggtaggcgac tccgctattg aggtcatcaa cgccacgact
9001 gggaaggatg agctgggccg cgcgtcccgc ctgtgtaagc acgcgttgta ctgtcgctgg
9061 atgcgtgtgc acggcaaggt tccctcccac ttactacgct ccaagattac caagcccaac
9121 gtgtaccatg agtccaagct ggcggcaaag gagtaccagg ccgccaaggc gcgtctgttc
9181 acagccttca tcaaggcggg gctgggggcc tgggtggaga agtccaccga gcaggaccag
9241 ttctcactca cgtaagcggc cgctcgagcc tagagggccc gtttaaaccc gctgatcagc
9301 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
9361 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
9421 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
9481 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
9541 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
9601 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
9661 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
9721 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
9781 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
9841 ccctttgacg ttggagtcca cgtt
//
