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pmKO2-N1载体 pmKO2N1质粒基本信息
pmKO2-N1质粒图谱 pmKO2N1载体图谱和pmKO2-N1载体序列 pmKO2N1质粒序列多克隆位点信息
pmKO2-N1质粒 pmKO2N1载体简介
pmKO2-N1质粒序列 pmKO2N1载体序列
LOCUS mKO2-N1 4669 bp ds-DNA circular SYN 20-JUN-2016 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS mKO2-N1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4669) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, September 1, 2016 from SnapGene Viewer 3.1.4 http://www.biofeng.com/ FEATURES Location/Qualifiers source 1..4669 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 16..319 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 320..523 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" misc_feature 546..626 /note="MCS" /note="multiple cloning site" CDS 634..1290 /codon_start=1 /product="monomeric Kusabira-Orange 2 fluorescent protein" /note="mKO2" /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS" polyA_signal 1412..1533 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(1540..1995) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2022..2126 /gene="bla" /note="AmpR promoter" promoter 2128..2485 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 2336..2471 /note="SV40 ori" /note="SV40 origin of replication" CDS 2520..3314 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3546..3593 /note="HSV TK poly(A) signal" /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 3922..4510 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa 61 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 121 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 181 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 241 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 301 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg 361 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg 421 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat 481 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca 541 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattct gcagtcgacg 601 gtaccgcggg cccgggatcc accggtcgcc accatggtga gtgtgattaa accagagatg 661 aagatgaggt actacatgga cggctccgtc aatgggcatg agttcacaat tgaaggtgaa 721 ggcacaggca gaccttacga gggacatcaa gagatgacac tacgcgtcac aatggccgag 781 ggcgggccaa tgcctttcgc gtttgactta gtgtcacacg tgttctgtta cggccacaga 841 gtatttacta aatatccaga agagatacca gactatttca aacaagcatt tcctgaaggc 901 ctgtcatggg aaaggtcgtt ggagttcgaa gatggtgggt ccgcttcagt cagtgcgcat 961 ataagcctta gaggaaacac cttctaccac aaatccaaat ttactggggt taactttcct 1021 gccgatggtc ctatcatgca aaaccaaagt gttgattggg agccatcaac cgagaaaatt 1081 actgccagcg acggagttct gaagggtgat gttacgatgt acctaaaact tgaaggaggc 1141 ggcaatcaca aatgccaaat gaagactact tacaaggcgg caaaagagat tcttgaaatg 1201 ccaggagacc attacatcgg ccatcgcctc gtcaggaaaa ccgaaggcaa cattactgag 1261 caggtagaag atgcagtagc tcatagctaa gcggccgcga ctctagatca taatcagcca 1321 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct 1381 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta 1441 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 1501 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt 1561 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa 1621 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag 1681 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg 1741 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga 1801 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg 1861 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg 1921 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc 1981 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 2041 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 2101 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg 2161 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 2221 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 2281 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 2341 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 2401 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 2461 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca 2521 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 2581 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 2641 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 2701 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 2761 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 2821 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 2881 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 2941 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 3001 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg 3061 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 3121 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 3181 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 3241 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 3301 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct 3361 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 3421 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 3481 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc 3541 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg 3601 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca 3661 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg 3721 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact 3781 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 3841 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 3901 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 3961 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 4021 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 4081 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 4141 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 4201 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 4261 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 4321 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 4381 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 4441 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 4501 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 4561 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat 4621 gcattagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccc //
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pmKO2-N1质粒 pmKO2N1载体应用举例
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