| 质粒名称: | gRNA_DNMT3a-T1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 41821 |
| 存储实验室: | George Church |
|
载体骨架基本信息 |
|
| 载体名称: | pCR-Blunt II-TOPO |
| 载体出品公司: | Invitrogen |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 未知 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | gRNA_DNMT3a-T1 |
| 物种来源: | H. sapiens (human) |
| 克隆方法: | TOPO Cloning |
| 5' 测序引物 | T7 |
|
文献引用方法 |
|
| 材料和方法部分: | gRNA_DNMT3a-T1 was a gift from George Church (Addgene plasmid # 41821) |
| 参考文献部分: | RNA-Guided Human Genome Engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM. Science. 2013 Jan 3. 10.1126/science.1232033 PubMed 23287722 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS gRNA_DNMT3a-T1 Sequencing Result 3974 bp DNA SYN
DEFINITION gRNA_DNMT3a-T1 Sequencing Result
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..3974
/organism="gRNA_DNMT3a-T1 Sequencing Result"
/mol_type="other DNA"
promoter 143..172
/label="lac_promoter"
misc_feature 186..208
/label="M13_pUC_rev_primer"
promoter 207..225
/label="M13_reverse_primer"
misc_feature 239..256
/label="Sp6_primer"
promoter complement(481..737)
/label="hU6_promoter"
misc_feature complement(532..551)
/label="LKO1_5_primer"
promoter complement(862..880)
/label="T7_promoter"
misc_feature 884..1027
/label="lacZ_a"
promoter complement(887..903)
/label="M13_forward20_primer"
misc_feature complement(896..918)
/label="M13_pUC_fwd_primer"
misc_feature 1050..1343
/label="ccdB"
promoter 1554..1603
/label="NEOKAN_promoter"
CDS 1692..2486
/label="ORF frame 3"
gene 1695..2483
/label="NeoR/KanR"
/gene="NeoR/KanR"
CDS complement(2001..2534)
/label="ORF frame 1"
promoter 2620..2648
/label="AmpR_promoter"
gene 2693..3064
/label="bleo"
/gene="bleo"
misc_feature 2693..3067
/label="sh_ble"
rep_origin 3189..3808
/label="pBR322_origin"
ORIGIN
1 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC
61 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC
121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA
181 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTAT
241 TTAGGTGACA CTATAGAATA CTCAAGCTAT GCATCAAGCT TGGTACCGAG CTCGGATCCA
301 CTAGTAACGG CCGCCAGTGT GCTGGAATTC GCCCTTTAAT GCCAACTTTG TACAAGAAAG
361 CTGGGTCTAG AAAAAAAGCA CCGACTCGGT GCCACTTTTT CAAGTTGATA ACGGACTAGC
421 CTTATTTTAA CTTGCTATTT CTAGCTCTAA AACCCTTGGG CCGCGCATCA TGCGGTGTTT
481 CGTCCTTTCC ACAAGATATA TAAAGCCAAG AAATCGAAAT ACTTTCAAGT TACGGTAAGC
541 ATATGATAGT CCATTTTAAA ACATAATTTT AAAACTGCAA ACTACCCAAG AAATTATTAC
601 TTTCTACGTC ACGTATTTTG TACTAATATC TTTGTGTTTA CAGTCAAATT AATTCTAATT
661 ATCTCTCTAA CAGCCTTGTA TCGTATATGC AAATATGAAG GAATCATGGG AAATAGGCCC
721 TCTTCCTGCC CGACCTTGGT ACCGGATCCA GTCGACTGAA TTGGTTCCTT TAAAGCCTGC
781 TTTTTTGTAC AAAGGGCGAA TTCTGCAGAT ATCCATCACA CTGGCGGCCG CTCGAGCATG
841 CATCTAGAGG GCCCAATTCG CCCTATAGTG AGTCGTATTA CAATTCACTG GCCGTCGTTT
901 TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC
961 CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT
1021 TGCGCAGCCT ATACGTACGG CAGTTTAAGG TTTACACCTA TAAAAGAGAG AGCCGTTATC
1081 GTCTGTTTGT GGATGTACAG AGTGATATTA TTGACACGCC GGGGCGACGG ATGGTGATCC
1141 CCCTGGCCAG TGCACGTCTG CTGTCAGATA AAGTCTCCCG TGAACTTTAC CCGGTGGTGC
1201 ATATCGGGGA TGAAAGCTGG CGCATGATGA CCACCGATAT GGCCAGTGTG CCGGTCTCCG
1261 TTATCGGGGA AGAAGTGGCT GATCTCAGCC ACCGCGAAAA TGACATCAAA AACGCCATTA
1321 ACCTGATGTT CTGGGGAATA TAAATGTCAG GCATGAGATT ATCAAAAAGG ATCTTCACCT
1381 AGATCCTTTT CACGTAGAAA GCCAGTCCGC AGAAACGGTG CTGACCCCGG ATGAATGTCA
1441 GCTACTGGGC TATCTGGACA AGGGAAAACG CAAGCGCAAA GAGAAAGCAG GTAGCTTGCA
1501 GTGGGCTTAC ATGGCGATAG CTAGACTGGG CGGTTTTATG GACAGCAAGC GAACCGGAAT
1561 TGCCAGCTGG GGCGCCCTCT GGTAAGGTTG GGAAGCCCTG CAAAGTAAAC TGGATGGCTT
1621 TCTCGCCGCC AAGGATCTGA TGGCGCAGGG GATCAAGCTC TGATCAAGAG ACAGGATGAG
1681 GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG
1741 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT
1801 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC
1861 TGAATGAACT GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT
1921 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG
1981 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG
2041 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG
2101 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG
2161 ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA
2221 GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA
2281 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC
2341 GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG
2401 CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT
2461 ATCGCCTTCT TGACGAGTTC TTCTGAATTA TTAACGCTTA CAATTTCCTG ATGCGGTATT
2521 TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATACA GGTGGCACTT TTCGGGGAAA
2581 TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT
2641 GAGACAATAA CCCTGATAAA TGCTTCAATA ATAGCACGTG AGGAGGGCCA CCATGGCCAA
2701 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG
2761 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG
2821 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT
2881 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC
2941 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG
3001 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA
3061 GGACTGACAC GTGCTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT
3121 TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC
3181 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT
3241 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC
3301 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT
3361 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT
3421 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA
3481 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC
3541 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG
3601 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT
3661 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC
3721 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG
3781 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGGCT TTTGCTGGCC
3841 TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC
3901 CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG
3961 CGAGGAAGCG GAAG
//
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