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pKD4载体质粒基本信息
pKD4质粒图谱载体图谱和pKD4载体序列质粒序列多克隆位点信息
pKD4质粒载体简介
pKD4质粒序列载体序列
LOCUS pKD4 3267 bp DNA SYN
DEFINITION pKD4
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..3267
/organism="pKD4"
/mol_type="other DNA"
misc_feature complement(51..98)
/label="FRT"
promoter 321..370
/label="NEOKAN_promoter"
CDS 459..1253
/label="ORF frame 3"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
CDS complement(768..1304)
/label="ORF frame 2"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
misc_feature 1882..1900
/label="pUni_fwd_primer"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
gene complement(1979..2839)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
CDS complement(1979..2839)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(2881..2909)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
terminator 3038..3081
/label="rrnB_T1_terminator"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
terminator 3213..3240
/label="rrnB_T2_terminator"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 agattgcagc attacacgtc ttgagcgatt gtgtaggctg gagctgcttc gaagttccta
61 tactttctag agaataggaa cttcggaata ggaacttcaa gatcccctca cgctgccgca
121 agcactcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc agtccgcaga
181 aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa
241 gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg
301 ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga
361 agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat
421 caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
481 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
541 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
601 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
661 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
721 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
781 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
841 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
901 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
961 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
1021 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
1081 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
1141 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
1201 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
1261 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
1321 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
1381 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccagct tcaaaagcgc
1441 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaacta aggaggatat
1501 tcatatggac catggctaat tcccatgtca gccgttaagt gttcctgtgt cactgaaaat
1561 tgctttgaga ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac
1621 cgttaaacct taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa
1681 accttagagg ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct
1741 tagtacgtga aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg
1801 agggtttagt tcgttaaaca tgagagctta gtacgttaaa catgagagct tagtacgtga
1861 aacatgagag cttagtacgt actatcaaca ggttgaactg cggatcttgc ggccgcaaaa
1921 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
1981 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
2041 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
2101 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
2161 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
2221 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
2281 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
2341 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
2401 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
2461 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
2521 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
2581 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
2641 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
2701 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
2761 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
2821 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
2881 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
2941 cgcgcacatt tccccgaaaa gtgccacctg catcgatggc cccccgatgg tagtgtgggg
3001 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
3061 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
3121 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg
3181 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt
3241 tgcgtggcca gtgccaagct tgcatgc
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