pKD4

LOCUS       pKD4	3267 bp 	DNA	SYN
DEFINITION  pKD4
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="pKD4"
                     /mol_type="other DNA"
     misc_feature    complement(51..98)
                     /label="FRT"
     promoter        321..370
                     /label="NEOKAN_promoter"
     CDS             459..1253
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(768..1304)
                     /label="ORF frame 2"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    1882..1900
                     /label="pUni_fwd_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     gene            complement(1979..2839)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     CDS             complement(1979..2839)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(2881..2909)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     terminator      3038..3081
                     /label="rrnB_T1_terminator"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     terminator      3213..3240
                     /label="rrnB_T2_terminator"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 agattgcagc attacacgtc ttgagcgatt gtgtaggctg gagctgcttc gaagttccta
   61 tactttctag agaataggaa cttcggaata ggaacttcaa gatcccctca cgctgccgca
  121 agcactcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc agtccgcaga
  181 aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa
  241 gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg
  301 ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga
  361 agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat
  421 caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
  481 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
  541 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
  601 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
  661 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
  721 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
  781 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
  841 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
  901 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
  961 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
 1021 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
 1081 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
 1141 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
 1201 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
 1261 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
 1321 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
 1381 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccagct tcaaaagcgc
 1441 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaacta aggaggatat
 1501 tcatatggac catggctaat tcccatgtca gccgttaagt gttcctgtgt cactgaaaat
 1561 tgctttgaga ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac
 1621 cgttaaacct taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa
 1681 accttagagg ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct
 1741 tagtacgtga aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg
 1801 agggtttagt tcgttaaaca tgagagctta gtacgttaaa catgagagct tagtacgtga
 1861 aacatgagag cttagtacgt actatcaaca ggttgaactg cggatcttgc ggccgcaaaa
 1921 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
 1981 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
 2041 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
 2101 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
 2161 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
 2221 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
 2281 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
 2341 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
 2401 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
 2461 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
 2521 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
 2581 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
 2641 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
 2701 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
 2761 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
 2821 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
 2881 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
 2941 cgcgcacatt tccccgaaaa gtgccacctg catcgatggc cccccgatgg tagtgtgggg
 3001 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
 3061 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
 3121 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg
 3181 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt
 3241 tgcgtggcca gtgccaagct tgcatgc
//