| 出品公司: | -- |
|---|---|
| 载体名称: | pKD20 |
| 质粒类型: | 细菌表达；基因敲除系统 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6078 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
买家导航
pKD20载体质粒基本信息
pKD20质粒图谱载体图谱和pKD20载体序列质粒序列多克隆位点信息
pKD20质粒载体简介
pKD20质粒序列载体序列
LOCUS AY048745 6078 bp DNA circular SYN 11-SEP-2001
DEFINITION Red recombinase plasmid pKD20, complete sequence.
ACCESSION AY048745
VERSION AY048745.1 GI:15554338
KEYWORDS .
SOURCE Red recombinase plasmid pKD20
ORGANISM Red recombinase plasmid pKD20
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6078)
AUTHORS Datsenko,K.A. and Wanner,B.L.
TITLE One-step inactivation of chromosomal genes in Escherichia coli K-12
using PCR products
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (12), 6640-6645 (2000)
PUBMED 10829079
REFERENCE 2 (bases 1 to 6078)
AUTHORS Datsenko,K.A. and Wanner,B.L.
TITLE Direct Submission
JOURNAL Submitted (29-JUL-2001) Biological Sciences, Purdue University,
Lilly Hall of Life Sciences, West Lafayette, IN 47907, USA
FEATURES Location/Qualifiers
source 1..6078
/organism="Red recombinase plasmid pKD20"
/mol_type="genomic DNA"
/db_xref="taxon:170494"
CDS complement(11..889)
/codon_start=1
/transl_table=11
/product="araC protein"
/protein_id="AAL02039.1"
/db_xref="GI:15554339"
misc_feature complement(1040..1068)
/note="Pc"
misc_feature 1165..1192
/note="ParaB"
CDS 1244..1660
/note="recombination;138"
/codon_start=1
/transl_table=11
/product="gam protein"
/protein_id="AAL02040.1"
/db_xref="GI:15554340"
CDS 1666..2451
/note="recombination;261"
/codon_start=1
/transl_table=11
/product="bet protein"
/protein_id="AAL02041.1"
/db_xref="GI:15554341"
CDS 2448..3128
/note="exo"
/codon_start=1
/transl_table=11
/product="exonuclease"
/protein_id="AAL02042.1"
/db_xref="GI:15554342"
CDS complement(3245..4195)
/codon_start=1
/transl_table=11
/product="repA101 protein"
/protein_id="AAL02043.1"
/db_xref="GI:15554343"
rep_origin 4199..4939
/note="oriR101"
CDS complement(5087..5947)
/note="bla; ampicillin resistance"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AAL02044.1"
/db_xref="GI:15554344"
ORIGIN
1 catcgattta ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
61 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
121 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
181 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
241 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
301 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
361 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
421 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
481 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg
541 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
601 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc
661 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca
721 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
781 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
841 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
901 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
961 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
1021 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
1081 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
1141 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
1201 acccgttttt ttgggaattc gagctctaag gaggttataa aaaatggata ttaatactga
1261 aactgagatc aagcaaaagc attcactaac cccctttcct gttttcctaa tcagcccggc
1321 atttcgcggg cgatattttc acagctattt caggagttca gccatgaacg cttattacat
1381 tcaggatcgt cttgaggctc agagctgggc gcgtcactac cagcagctcg cccgtgaaga
1441 gaaagaggca gaactggcag acgacatgga aaaaggcctg ccccagcacc tgtttgaatc
1501 gctatgcatc gatcatttgc aacgccacgg ggccagcaaa aaatccatta cccgtgcgtt
1561 tgatgacgat gttgagtttc aggagcgcat ggcagaacac atccggtaca tggttgaaac
1621 cattgctcac caccaggttg atattgattc agaggtataa aacgaatgag tactgcactc
1681 gcaacgctgg ctgggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa
1741 ctgatcacca ctcttcgcca gacggcattt aaaggtgatg ccagcgatgc gcagttcatc
1801 gcattactga tcgttgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc
1861 tttcctgata agcagaatgg catcgttccg gtggtgggcg ttgatggctg gtcccgcatc
1921 atcaatgaaa accagcagtt tgatggcatg gactttgagc aggacaatga atcctgtaca
1981 tgccggattt accgcaagga ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa
2041 tgccgccgcg aaccattcaa aactcgcgaa ggcagagaaa tcacggggcc gtggcagtcg
2101 catcccaaac ggatgttacg tcataaagcc atgattcagt gtgcccgtct ggccttcgga
2161 tttgctggta tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact
2221 gcagaacgtc agccggaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt
2281 aacactctgc tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc
2341 cagatatttc gccgcgacat tcgtgcatcg tcagaactga cacaggccga agcagtaaaa
2401 gctcttggat tcctgaaaca gaaagccgca gagcagaagg tggcagcatg acaccggaca
2461 ttatcctgca gcgtaccggg atcgatgtga gagctgtcga acagggggat gatgcgtggc
2521 acaaattacg gctcggcgtc atcaccgctt cagaagttca caacgtgata gcaaaacccc
2581 gctccggaaa gaagtggcct gacatgaaaa tgtcctactt ccacaccctg cttgctgagg
2641 tttgcaccgg tgtggctccg gaagttaacg ctaaagcact ggcctgggga aaacagtacg
2701 agaacgacgc cagaaccctg tttgaattca cttccggcgt gaatgttact gaatccccga
2761 tcatctatcg cgacgaaagt atgcgtaccg cctgctctcc cgatggttta tgcagtgacg
2821 gcaacggcct tgaactgaaa tgcccgttta cctcccggga tttcatgaag ttccggctcg
2881 gtggtttcga ggccataaag tcagcttaca tggcccaggt gcagtacagc atgtgggtga
2941 cgcgaaaaaa tgcctggtac tttgccaact atgacccgcg tatgaagcgt gaaggcctgc
3001 attatgtcgt gattgagcgg gatgaaaagt acatggcgag ttttgacgag atcgtgccgg
3061 agttcatcga aaaaatggac gaggcactgg ctgaaattgg ttttgtattt ggggagcaat
3121 ggcgatgata agctagccca tgggtatgga cagttttccc tttgatatgt aacggtgaac
3181 agttgttcta cttttgtttg ttagtcttga tgcttcactg atagatacaa gagccataag
3241 aacctcagat ccttccgtat ttagccagta tgttctctag tgtggttcgt tgtttttgcg
3301 tgagccatga gaacgaacca ttgagatcat acttactttg catgtcactc aaaaattttg
3361 cctcaaaact ggtgagctga atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc
3421 cgttacgtag gtaggaatct gatgtaatgg ttgttggtat tttgtcacca ttcattttta
3481 tctggttgtt ctcaagttcg gttacgagat ccatttgtct atctagttca acttggaaaa
3541 tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa tttcatattg ctgtaagtgt
3601 ttaaatcttt acttattggt ttcaaaaccc attggttaag ccttttaaac tcatggtagt
3661 tattttcaag cattaacatg aacttaaatt catcaaggct aatctctata tttgccttgt
3721 gagttttctt ttgtgttagt tcttttaata accactcata aatcctcata gagtatttgt
3781 tttcaaaaga cttaacatgt tccagattat attttatgaa tttttttaac tggaaaagat
3841 aaggcaatat ctcttcacta aaaactaatt ctaatttttc gcttgagaac ttggcatagt
3901 ttgtccactg gaaaatctca aagcctttaa ccaaaggatt cctgatttcc acagttctcg
3961 tcatcagctc tctggttgct ttagctaata caccataagc attttcccta ctgatgttca
4021 tcatctgagc gtattggtta taagtgaacg ataccgtccg ttctttcctt gtagggtttt
4081 caatcgtggg gttgagtagt gccacacagc ataaaattag cttggtttca tgctccgtta
4141 agtcatagcg actaatcgct agttcatttg ctttgaaaac aactaattca gacatacatc
4201 tcaattggtc taggtgattt taatcactat accaattgag atgggctagt caatgataat
4261 tactagtcct tttcctttga gttgtgggta tctgtaaatt ctgctagacc tttgctggaa
4321 aacttgtaaa ttctgctaga ccctctgtaa attccgctag acctttgtgt gttttttttg
4381 tttatattca agtggttata atttatagaa taaagaaaga ataaaaaaag ataaaaagaa
4441 tagatcccag ccctgtgtat aactcactac tttagtcagt tccgcagtat tacaaaagga
4501 tgtcgcaaac gctgtttgct cctctacaaa acagacctta aaaccctaaa ggcttaagta
4561 gcaccctcgc aagctcggtt gcggccgcaa tcgggcaaat cgctgaatat tccttttgtc
4621 tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac
4681 ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga ttcatgcaag
4741 gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg
4801 tctgctatgt ggtgctatct gactttttgc tgttcagcag ttcctgccct ctgattttcc
4861 agtctgacca cttcggatta tcccgtgaca ggtcattcag actggctaat gcacccagta
4921 aggcagcggt atcatcaacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
4981 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
5041 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
5101 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
5161 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
5221 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
5281 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
5341 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
5401 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
5461 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
5521 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
5581 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
5641 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
5701 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
5761 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
5821 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
5881 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
5941 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
6001 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
6061 cccgaaaagt gccacctg
//
