LOCUS pSABEn 13805 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses ABEn CRISPR base editor and gRNA scaffold in Streptomyces.
ACCESSION .
VERSION .
KEYWORDS pSABEn
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13805)
AUTHORS Zhong Z, Guo J, Deng L, Chen L, Wang J, Li S, Xu W, Deng Z, Sun Y
TITLE Base editing in Streptomyces with Cas9-deaminase fusions
JOURNAL BioRxiv 630137
REFERENCE 2 (bases 1 to 13805)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13805
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(250..270)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(250..268)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(297..315)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 383..487
/gene="bla"
/label=AmpR promoter
CDS 488..1348
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(706..725)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1519..2107
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2008..2027
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2261..2278
/label=L4440
/note="L4440 vector, forward primer"
polyA_signal 2492..2626
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2516..2535)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2570..2589
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
promoter 5844..6145
/label=rpsL promoter
/note="promoter for the Xylanimonas cellulosilytica
ribosomal protein S12 gene"
CDS 7337..11440
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
oriT 11803..11912
/note="incP origin of transfer"
CDS 11945..12316
/codon_start=1
/gene="traJ"
/product="oriT-recognizing protein"
/label=traJ
/translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP"
misc_RNA 12472..12547
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
CDS complement(13061..56)
/codon_start=1
/gene="aac(3)IV"
/product="aminoglycoside 3-N-acetyltransferase type IV
"
/label=ApmR
/note="confers resistance to apramycin (Paget and Davies,
1996)"
/translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
ORIGIN
1 agctcggctt ttcgccattc gtattgcacg acattgcact ccaccgctga tgacatcagt
61 cgatcatagc acgatcaacg gcactgttgc aaatagtcgg tggtgataaa cttatcatcc
121 ccttttgctg atggagctgc acatgaaccc attcaaaggc cggcattttc agcgtgacat
181 cattctgtgg gccgtacgct ggtactgcaa atacggcatc agttaccgtg agctgcaggt
241 cgacggatcc ctttagtgag ggttaattgc ggccgcgaat tcttgaagac gaaagggcct
301 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
361 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
421 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
481 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
541 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
601 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
661 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
721 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
781 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
841 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
901 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
961 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
1021 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
1081 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
1141 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
1201 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
1261 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
1321 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
1381 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
1441 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
1501 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
1561 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
1621 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
1681 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
1741 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
1801 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
1861 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
1921 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
1981 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
2041 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
2101 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
2161 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
2221 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
2281 agcggaagag cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca
2341 ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc
2401 gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca
2461 acgacaggag cacgatcatg cgcacccgtc agatccagac atgataagat acattgatga
2521 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
2581 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttcccg cacgatcagc
2641 gtcccgccga ccaggagcag tgcggccagc actgccgcta acgcctggtc ctggtcccgg
2701 tcctggtggt gcatcagtcc tccccgtgat cacttcggca cccaccgtag tgatcacccc
2761 cgacagcgga tcaaggggtt tgcgggtccc ggtcggcgcc gggcggggga ggcaggagcc
2821 gccgacgctg cctctgggac gggccggacg gcagggggac cggcggccgg gcgagctgca
2881 gccgggggtc cggcagggcc ggagcgggcg gaaccgtgct ctgacctgcg gcccgagttt
2941 cgtcacgtga cggaatggaa ggctgctgca tttcgtcacg tgacgtatct cggcgagcga
3001 ctgccgacgc cacggcggac acgatcgcct cgcgctggcg ccgggcctcg tacgcccgct
3061 ggcggcagga gcggcggcag tagtcccggc tccggccgac gccggattgc ttgatctccg
3121 agccgcacca ggcgcagagc ttcgcgccgt cggcgtccct gggggtggtg gtgctcatgg
3181 ccgacgaccg tacgcggcac gtctcgtagc gaggcgagtc gggcgcgagg taccgcctgc
3241 acgaagtgcc ggcggggccg accccgggcg agtaatccca ggattactcc cgcggcttcg
3301 accccggccg ccgtcgccgc gtacgtcacc gacccccgcc gtacgtcacc gggatgacgt
3361 acggcggggg ggagcgagtt agtgcgaagt gggcccactt gcgagccggg cgatgtgccg
3421 ggcggcccgc tcctggcggt cgtcggcgtc gtcgtcctgg tcgtcgtcct gctctcgccg
3481 tcggcgtgca gttgcttcct cgcggcgctg ggcgagggcg gcgagcatgt cggcgtacgc
3541 ctcggccacc tcccccgccg tgagcaccac cactgtgtcg gccgcgtcgg ccagcgccag
3601 gacctcccgc acccgttcgc ccacggccgc cgaatcctcg ttgccgtcct tgccttcggc
3661 ggcccgggtc gcctcgaggt cgagggcgcg gcgggtgacc gcgtgccatc cgtcctcggt
3721 cacggcgacc ccggcccgca gctccccgcc gtcggcgtcg gccgccagga gcagatcgag
3781 gtcgtcggcc tcggtgtcgc cgccgtcgag cccgagcatc tgccgcaggt agcgggtcca
3841 ttcgatggcc cggcgtcccc gggttgcccg ctcgtactcg tgccagcgcg agaggttcca
3901 ctccagcgag ccgaccccgg cggcgtcgtc ctcggtcatg ccgccggtca ggtccccgat
3961 ccgtccgagg agttcgaacg gggcgacgtt cccgccggtc gccgtcttga ggtcggcgcg
4021 ggcgagttcg agggcgggcg ccttcccgtc ctgggtcttg gcgatgtact cggcgaggtc
4081 gttggcgtcg cgctcggtct ccagccgctt gaagtcgacg ccgtgccggt cgtcgggcgt
4141 gaaggcgggg ttgaccttgc gcagggcggc ggtccacacg gaccgccagt gcccctgcca
4201 ctcgtcgagc gcggcgccgg tcggctcgaa ggtggcgacg atctgcttcg cggaccgctc
4261 cccctcggtc cggccgccga ccaggacgat cgcgtggatg tgcgggtgcc agccgttgat
4321 ctgccccacg gtgacttcgg tcgcgcggat catgccgacg tacccgatcc ggtctcggat
4381 gccctcgcgg tcggcggccc ggtgcccgtc cttggcccgg cgtccggccc acgtgccgcc
4441 cgtgatcagt cgctggtagg cgcccggccg ccgggggctg tccggcgtct tccgggtgcc
4501 ctggagggcg tccatgaggt ccgcgagccg gtccgtgtgc ccatggcggg ccgtgaaggt
4561 gaccaggtag gcggtccccc cgcgcttgat ccactcgacc acggcggcgg tgatctcctc
4621 ggcccgcttg tgccggatcg tggcggcgca gaccgggcag agccagatcc gcccgcaccg
4681 catcaggccc aggaccacgg acgttccggc cgccgtctgg gcgacgatca cgccggaggc
4741 agggtccatc agggcgcggc cgcagccctt gcacgcggcg tccccgctga tccgccacag
4801 cgtccggcgg cggctgtacc gggcggcttt ccgcagtcgg gcagcgtcgc tccgcgacgt
4861 gcttcctact tccgagaggc tgtcgcctct cgggctctcc ccatccaccc cgtccggaga
4921 aaccgcaggt cggaggggtg cgggaaactc tgttgtttct ttcccaaggt gttcgctttt
4981 gcctcgggcg gcatctcgcg tcacacgcgc gatcgcccgc ttcgctgcca tccggcagcg
5041 gtctgagcag tagatacgcg gccgtttgcc cggtgtgtgg gcaattgcgg tcccgcagtg
5101 gcagcggggc ccggcgggcc gatctggcaa tgcctcggca tcgctccgta ctctgggcac
5161 gagcaacgtt cctgtctcgc ccggctaagg ggcgcgagtc tgggagcgga cgggtcggag
5221 gtgcgaagtc cggcccgttg ctctttggtc tggtgggaat cctggcacca atcgggccag
5281 aggttccctc cgccactccc gacgcccctt ggggctggtg tgacttggag ggccgaagag
5341 agccccgccg gtgatccggc ggggctttga cgtgcggtca gtgcgtgtgt cggcgagcga
5401 tggccacgag gccctggaag ccgagcggtc cggcgaagtc ggcccagtcg caaccgggct
5461 cagcgcagtg ggcggaccag ccaccgccgt tggggtcctg gaccaggttc acggtcccct
5521 cggtcaggcg tccgtcgaag tcggtcatgg tcggtctcct ggtgggtggg ggcggggcgc
5581 cagcacgaag tgccggcgcc ccgcgggggt tggtcgggtc aggcgccgaa ccggcgggcg
5641 gcggcggcga ccaggccgtc ggcggcggcc atggcgcggt cgcggtcggt ggtgagggcg
5701 gtgcggtcgg cggcggccca gtcctgctct cacgcaacgt ctacgtggac gctcagggcg
5761 acacgatcga ggtcggggag tccgtcgctc cggcgggccg ctggcgcgtc caccgggact
5821 gatcaaggcg aatacttcat atggccctgc aggcggaagt caggtagaca cgacttccgc
5881 tagtccttgc aaggtctgct gacgtgaggc ggggcggtcg tttttgaccg ccctgccttc
5941 gtcatgtagg ctcgctcgct gtgcctggcg tgtcatcaga cgcccaggtc ccggtgccgt
6001 gaggcccggg ccatcgagcc ggtggtacgt ggctgcggtc cccttgtgag ggctgcgcgc
6061 cgtgtgctgt ccggcgcgca cagccttgaa tccacccgcg ggggccggcc ggtctccgtg
6121 agctcgagta gacgacggag acgtaatgtc cgaggtggag ttctcccacg agtactggat
6181 gcgccacgcc ctgaccctgg ccaagcgcgc gtgggacgag cgcgaggtgc cggtgggcgc
6241 cgtgctggtc cacaacaacc gcgtgatcgg cgagggctgg aaccggccga tcggccggca
6301 cgacccgacc gcccacgccg agatcatggc cctgcgccag ggcggcctgg tcatgcagaa
6361 ctaccggctg atcgacgcga ccctgtacgt gaccctggag ccgtgcgtca tgtgcgcggg
6421 cgccatgatc cactcccgca tcggccgggt ggtgttcggc gcccgggacg ccaagaccgg
6481 cgccgcgggc tccctgatgg acgtgctgca ccacccgggc atgaaccacc gcgtcgagat
6541 caccgagggc atcctggcgg acgagtgcgc cgcgctgctg tcggacttct tccggatgcg
6601 ccggcaggag atcaaggccc agaagaaggc gcagtcgtcc accgactcgg gcggctcgtc
6661 cggcggctcg tccggctccg agaccccggg cacctcggag tccgcgaccc cggagtcgtc
6721 cggcggctcg tccggcggct cgtccgaggt cgaattttcc catgaatact ggatgcggca
6781 cgcgctgacc ctggccaagc gggcccgcga cgagcgcgag gtgcccgtcg gcgcggtgct
6841 ggtcctgaac aaccgggtca ttggtgaggg ctggaaccgc gccatcggcc tgcacgaccc
6901 caccgcgcat gccgaaatca tggccctccg gcagggcggc ctggtgatgc agaactaccg
6961 cctcatcgac gccaccctgt acgtgacctt cgagccctgc gtgatgtgtg cgggtgccat
7021 gatccactcc cggatcggcc gcgtcgtgtt cggcgtgcgc aacgccaaga ccggcgcggc
7081 cggctccctg atggacgtcc tgcactaccc cggcatgaac caccgcgtgg aaattaccga
7141 gggcatcctg gccgacgagt gcgcggccct gctgtgctac ttcttccgca tgccgcggca
7201 ggtgttcaac gcgcagaaga aggcgcagtc gtccacggat tcgggcggct cgtccggcgg
7261 ctcgtccggg tcggaaaccc cgggtacctc ggagtcggcc accccggagt cgtccggcgg
7321 ctcgtccggc ggctcggaca agaagtacag catcggcctg gccatcggca ccaacagcgt
7381 gggctgggcg gtcatcaccg acgagtacaa ggtcccctcc aagaagttca aggtcctggg
7441 caacaccgac cggcactcga tcaagaagaa cctgatcggc gccctgctct tcgacagcgg
7501 cgagaccgcc gaggcgaccc gcctgaagcg gaccgcgcgc cggcgctaca cccggcgcaa
7561 gaaccgcatc tgctacctcc aggaaatctt ctccaacgag atggccaagg tcgacgactc
7621 gttcttccac cggctcgagg agagcttcct ggtggaggag gacaagaagc acgagcgcca
7681 cccgatcttc ggcaacatcg tcgacgaggt ggcctaccac gagaagtacc ccaccatcta
7741 ccacctccgc aagaagctgg tcgactcgac cgacaaggcg gacctgcggc tcatctacct
7801 ggccctcgcg cacatgatca agttccgcgg ccacttcctc atcgagggcg acctgaaccc
7861 ggacaactcc gacgtcgaca agctcttcat ccagctggtg cagacctaca accagctgtt
7921 cgaggagaac cccatcaacg ccagcggcgt cgacgccaag gcgatcctct ccgcgcgcct
7981 gagcaagtcc cggcgcctgg agaacctcat cgcccagctg ccgggcgaga agaagaacgg
8041 cctcttcggc aacctgatcg cgctgtcgct cggcctgacc cccaacttca agagcaactt
8101 cgacctggcc gaggacgcga agctccagct gtccaaggac acctacgacg acgacctgga
8161 caacctgctc gcccagatcg gcgaccagta cgcggacctc ttcctggccg cgaagaacct
8221 ctcggacgcc atcctgctca gcgacatcct gcgggtcaac accgagatca ccaaggcccc
8281 gctgtcggcg agcatgatca agcggtacga cgagcaccac caggacctga ccctgctcaa
8341 ggccctcgtg cgccagcagc tgcccgagaa gtacaaggaa atcttcttcg accagtccaa
8401 gaacggctac gccggctaca tcgacggcgg cgcgtcgcag gaggagttct acaagttcat
8461 caagccgatc ctggagaaga tggacggcac cgaggagctg ctcgtcaagc tgaaccgcga
8521 ggacctgctc cgcaagcagc ggaccttcga caacggctcc atcccgcacc agatccacct
8581 gggcgagctg cacgccatcc tccggcgcca ggaggacttc taccccttcc tgaaggacaa
8641 ccgcgagaag atcgagaaga tcctgacctt ccgcatcccg tactacgtcg gccccctggc
8701 ccgcggcaac tcccggttcg cgtggatgac ccggaagtcg gaggagacca tcaccccgtg
8761 gaacttcgag gaggtcgtgg acaagggcgc ctccgcccag tcgttcatcg agcgcatgac
8821 caacttcgac aagaacctcc cgaacgagaa ggtcctgccc aagcactccc tgctctacga
8881 gtacttcacc gtgtacaacg agctgaccaa ggtcaagtac gtgaccgagg gcatgcggaa
8941 gccggccttc ctgtcgggcg agcagaagaa ggcgatcgtc gacctgctct tcaagaccaa
9001 ccgcaaggtc accgtgaagc agctgaagga ggactacttc aagaagatcg agtgcttcga
9061 ctccgtcgag atcagcggcg tggaggaccg cttcaacgcc tccctgggca cctaccacga
9121 cctgctcaag atcatcaagg acaaggactt cctcgacaac gaggagaacg aggacatcct
9181 ggaggacatc gtcctcaccc tgaccctctt cgaggaccgc gagatgatcg aggagcggct
9241 caagacctac gcccacctgt tcgacgacaa ggtgatgaag cagctgaagc ggcgccggta
9301 caccggctgg ggccgcctct cccggaagct gatcaacggc atccgggaca agcagagcgg
9361 caagaccatc ctggacttcc tcaagtccga cggcttcgcc aaccgcaact tcatgcagct
9421 catccacgac gacagcctga ccttcaagga ggacatccag aaggcccagg tctcgggcca
9481 gggcgacagc ctccacgagc acatcgccaa cctggcgggc tccccggcga tcaagaaggg
9541 catcctccag accgtcaagg tcgtggacga gctggtcaag gtgatgggcc gccacaagcc
9601 cgagaacatc gtgatcgaga tggcccggga gaaccagacc acccagaagg gccagaagaa
9661 ctcgcgcgag cggatgaagc ggatcgagga gggcatcaag gagctgggca gccagatcct
9721 gaaggagcac ccggtcgaga acacccagct ccagaacgag aagctgtacc tctactacct
9781 ccagaacggc cgcgacatgt acgtggacca ggagctggac atcaaccggc tgtccgacta
9841 cgacgtcgac cacatcgtgc cgcagtcctt cctgaaggac gactcgatcg acaacaaggt
9901 cctgacccgc tcggacaaga accggggcaa gtccgacaac gtgccctcgg aggaggtcgt
9961 gaagaagatg aagaactact ggcgccagct gctcaacgcc aagctcatca cccagcgcaa
10021 gttcgacaac ctgaccaagg ccgagcgggg cggcctgagc gagctggaca aggcgggctt
10081 catcaagcgc cagctggtcg agacccggca gatcaccaag cacgtggccc agatcctgga
10141 ctcccggatg aacaccaagt acgacgagaa cgacaagctg atccgcgagg tcaaggtgat
10201 caccctcaag agcaagctgg tctccgactt ccgcaaggac ttccagttct acaaggtccg
10261 ggagatcaac aactaccacc acgcccacga cgcgtacctg aacgccgtcg tgggcaccgc
10321 gctgatcaag aagtacccga agctggagtc cgagttcgtc tacggcgact acaaggtcta
10381 cgacgtgcgc aagatgatcg ccaagagcga gcaggagatc ggcaaggcca ccgcgaagta
10441 cttcttctac tccaacatca tgaacttctt caagaccgag atcaccctgg ccaacggcga
10501 gatccgcaag cggcccctga tcgagaccaa cggcgagacc ggcgagatcg tctgggacaa
10561 gggccgcgac ttcgccaccg tccggaaggt gctgtcgatg ccgcaggtca acatcgtgaa
10621 gaagaccgag gtgcagaccg gcggcttcag caaggagtcc atcctcccca agcgcaacag
10681 cgacaagctg atcgcccgga agaaggactg ggacccgaag aagtacggcg gcttcgacag
10741 ccccaccgtc gcctactccg tgctggtcgt ggcgaaggtc gagaagggca agagcaagaa
10801 gctgaagtcc gtgaaggagc tgctcggcat caccatcatg gagcgctcct cgttcgagaa
10861 gaacccgatc gacttcctgg aggccaaggg ctacaaggag gtcaagaagg acctcatcat
10921 caagctgccc aagtacagcc tgttcgagct ggagaacggc cgcaagcgga tgctcgcctc
10981 cgcgggcgag ctgcaaaagg gcaacgagct ggccctcccg tcgaagtacg tcaacttcct
11041 gtacctcgcg tcccactacg agaagctgaa gggctcgccc gaggacaacg agcagaagca
11101 gctcttcgtg gagcagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt
11161 cagcaagcgc gtcatcctgg ccgacgcgaa cctcgacaag gtgctgtccg cctacaacaa
11221 gcaccgcgac aagccgatcc gggagcaggc ggagaacatc atccacctgt tcaccctcac
11281 caacctgggt gccccggccg ccttcaagta cttcgacacc accatcgacc gcaagcggta
11341 cacctccacc aaggaggtcc tcgacgcgac cctgatccac cagagcatca ccggcctgta
11401 cgagacccgc atcgacctga gccagctggg cggcgactga gaattcagat ctacgcgttc
11461 cccgcaaaag cggcctttga ctccctgcaa gcctcagcga ccgaatatat cggttatgcg
11521 tgggcgatgg ttgttgtcat tgtcggcgca actatcggta tcaagctgtt taagaaattc
11581 acctcgaaag caagctgata aaccgataca attaaaggct ccttttggag ccttttttta
11641 agcttgcatg cctgcaggtc gacggatctt ttccgctgca taaccctgct tcggggtcat
11701 tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat tttgccaaag
11761 ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga taggtgaagt
11821 aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc tggcggtgct
11881 caacgggaat cctgctctgc gaggctggcc ggctaccgcc ggcgtaacag atgagggcaa
11941 gcggatggct gatgaaacca agccaaccag gaagggcagc ccacctatca aggtgtactg
12001 ccttccagac gaacgaagag cgattgagga aaaggcggcg gcggccggca tgagcctgtc
12061 ggcctacctg ctggccgtcg gccagggcta caaaatcacg ggcgtcgtgg actatgagca
12121 cgtccgcgag ctggcccgca tcaatggcga cctgggccgc ctgggcggcc tgctgaaact
12181 ctggctcacc gacgacccgc gcacggcgcg gttcggtgat gccacgatcc tcgccctgct
12241 ggcgaagatc gaagagaagc aggacgagct tggcaaggtc atgatgggcg tggtccgccc
12301 gagggcagag ccatgacttt tttagccgct aaaacggccg gggggtgcgc gtgattgcca
12361 agcacgtccc catgcgctcc atcaagaaga gcgacttcgc ggagctggtg aagtacatca
12421 ccgacgagca aggcaagacc gatccccggg gacctgcagg tcgactctag agttttagag
12481 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
12541 tcggtgctaa aaaaaaaccc cgcccctgac agggcggggt tttttttgta gcctcaggag
12601 ttgtagctgt tcacattcga acggtctctg ctttgacaac atgctgtgcg gtgttgtaaa
12661 gtcgtggccg ctagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
12721 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
12781 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc
12841 cccggacgag cgtctgctcc gccattcgcc gtccgccgtg ccaatcggat cagccgtcca
12901 aatgcgggat tttcgttagt cggaggccaa acggcattga gcgtcagcat atcatcagcg
12961 agctgaagaa agacaatccc cgatccgctc cacgtgttgc cccagcaatc agcgcgacct
13021 tgcccctcca acgtcatctc gttctccgct catgagctca gccaatcgac tggcgagcgg
13081 catcgcattc ttcgcatccc gcctctggcg gatgcaggaa gatcaacgga tctcggccca
13141 gttgacccag ggctgtcgcc acaatgtcgc gggagcggat caaccgagca aaggcatgac
13201 cgactggacc ttccttctga aggctcttct ccttgagcca cctgtccgcc aaggcaaagc
13261 gctcacagca gtggtcattc tcgagataat cgacgcgtac caacttgcca tcctgaagaa
13321 tggtgcagtg tctcggcacc ccatagggaa cctttgccat caactcggca agatgcagcg
13381 tcgtgttggc atcgtgtccc acgccgagga gaagtacctg cccatcgagt tcatggacac
13441 gggcgaccgg gcttgcaggc gagtgaggtg gcaggggcaa tggatcagag atgatctgct
13501 ctgcctgtgg ccccgctgcc gcaaaggcaa atggatgggc gctgcgcttt acatttggca
13561 ggcgccagaa tgtgtcagag acaactccaa ggtccggtgt aacgggcgac gtggcaggat
13621 cgaacggctc gtcgtccaga cctgaccacg agggcatgac gagcgtccct cccggaccca
13681 gcgcagcacg cagggcctcg atcagtccaa gtggcccatc ttcgaggggc cggacgctac
13741 ggaaggagct gtggaccagc agcacaccgc cgggggtaac cccaaggttg agaagctgac
13801 cgatg
//
