LOCUS TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_P2A_CD3zeta 12681 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION 2nd generation lentiviral vector which expresses SNAP tagged TCRbeta
and other TCR subunits (Jurkat alleles) separated via 2A sequences.
ACCESSION .
VERSION .
KEYWORDS TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_P2A_CD3zeta
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12681)
AUTHORS Taylor MJ, Husain K, Gartner ZJ, Mayor S, Vale RD
TITLE A DNA-Based T Cell Receptor Reveals a Role for Receptor Clustering
in Ligand Discrimination.
JOURNAL Cell. 2017 Mar 23;169(1):108-119.e20. doi:
10.1016/j.cell.2017.03.006.
PUBMED 28340336
REFERENCE 2 (bases 1 to 12681)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12681
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 17..605
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 506..525
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 759..776
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(846..866)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 851..1180
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 1031..1166
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 1093..1112
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 2314..2379
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 2509..2529
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 2954..3088
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2991..3010)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3045..3064
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 3256..3889
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 3936..4061
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 4558..4791
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 4976..5020
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 5305..5422
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 5575..5982
/label=SFFV promoter
/note="spleen focus-forming virus long terminal repeat
(LTR) promoter"
CDS 6909..6968
/codon_start=1
/product="2A peptide from equine rhinitis A virus
polyprotein"
/label=E2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="QCTNYALLKLAGDVESNPGP"
CDS 7044..7586
/codon_start=1
/product="human O6-alkylguanine-DNA-alkyltransferase
variant that forms covalent bonds with benzylguanine
derivatives"
/label=SNAP-tag(R)
/note="fast-reacting variant, optimized for mammalian
expression"
/translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
KEWLLAHEGHRLGKPGLG"
CDS 8487..8543
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 9186..9239
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
misc_feature 9800..10388
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(9853..9873)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(10271..10282)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(10391..10407)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10392..10408)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 10498..10731
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(10756..10774)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(10757..10774)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(11064..11083)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(11297..11316)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 11416..11438
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(11476..11494)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 11562..11666
/gene="bla"
/label=AmpR promoter
CDS 11667..12527
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(11885..11904)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
ORIGIN
1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
781 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
841 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
901 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
961 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
1021 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
1081 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
1141 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg
1201 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
1261 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
1321 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
1381 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
1441 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt
1501 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
1561 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
1621 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
1681 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
1741 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
1801 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
1861 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
1921 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
1981 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
2041 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
2101 ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
2161 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga
2221 tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
2281 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
2341 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
2401 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
2461 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
2521 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
2581 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
2641 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
2701 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
2761 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
2821 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
2881 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
2941 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
3001 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
3061 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
3121 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
3181 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
3241 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
3301 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
3361 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
3421 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
3481 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
3541 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
3601 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
3661 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
3721 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
3781 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
3841 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
3901 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
3961 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
4021 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
4081 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
4141 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
4201 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
4261 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
4321 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
4381 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
4441 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
4501 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
4561 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
4621 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
4681 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
4741 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
4801 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
4861 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
4921 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
4981 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
5041 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
5101 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
5161 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
5221 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
5281 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
5341 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
5401 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggat
5461 cgataagctt gatatcgaat tcctgcagcc ccgataaaat aaaagatttt atttagtctc
5521 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
5581 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
5641 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
5701 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
5761 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
5821 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
5881 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
5941 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggggggatct ggagctctcg
6001 agaattctca cgcgtctgca ggatatcaag cttgcggtac cgcgggcccg ggatctgcca
6061 ccatgctcct gctgctcgtc ccagtgctgg aggtgatttt taccctggga ggaaccagag
6121 cccagtcggt gacccagctt ggcagccacg tctctgtctc tgaaggagcc ctggttctgc
6181 tgaggtgcaa ctactcatcg tctgttccac catatctctt ctggtatgtg caatacccca
6241 accaaggact ccagcttctc ctgaagtaca catcagcggc caccctggtt aaaggcatca
6301 acggttttga ggctgaattt aagaagagtg aaacctcctt ccacctgaca aaaccctcag
6361 cccatatgag cgacgcggct gagtacttct gtgctgtgag tgatctcgaa ccgaacagca
6421 gtgcttccaa gataatcttt ggatcaggga ccagactcag catccggcca aatatccaga
6481 accctgaccc tgccgtgtac cagctgagag actctaaatc cagtgacaag tctgtctgcc
6541 tattcaccga ttttgattct caaacaaatg tgtcacaaag taaggattct gatgtgtata
6601 tcacagacaa aactgtgcta gacatgaggt ctatggactt caagagcaac agtgctgtgg
6661 cctggagcaa caaatctgac tttgcatgtg caaacgcctt caacaacagc attattccag
6721 aagacacctt cttccccagc ccagaaagtt cctgtgatgt caagctggtc gagaagagct
6781 ttgaaacaga tacgaaccta aactttcaaa acctgtcagt gattgggttc cgaatcctcc
6841 tcctgaaagt ggccgggttt aatctgctca tgacgctgcg gctgtggtcc agcgaattcg
6901 ggtccggcca atgtactaac tacgctttgt tgaaactcgc tggcgatgtt gaaagtaacc
6961 ccggtcctaa gcttatgcag tcgggcactc actggagagt tctgggcctc tgcctcttat
7021 cagttggcgt ttgggggcaa gatgacaaag actgcgaaat gaagcgcacc accctggata
7081 gccctctggg caagctggaa ctgtctgggt gcgaacaggg cctgcaccgt atcatcttcc
7141 tgggcaaagg aacatctgcc gccgacgccg tggaagtgcc tgccccagcc gccgtgctgg
7201 gcggaccaga gccactgatg caggccaccg cctggctcaa cgcctacttt caccagcctg
7261 aggccatcga ggagttccct gtgccagccc tgcaccaccc agtgttccag caggagagct
7321 ttacccgcca ggtgctgtgg aaactgctga aagtggtgaa gttcggagag gtcatcagct
7381 acagccacct ggccgccctg gccggcaatc ccgccgccac cgccgccgtg aaaaccgccc
7441 tgagcggaaa tcccgtgccc attctgatcc cctgccaccg ggtggtgcag ggcgacctgg
7501 acgtgggggg ctacgagggc gggctcgccg tgaaagagtg gctgctggcc cacgagggcc
7561 acagactggg caagcctggg ctgggtggtt ccggatcagt ggttatccag tcaccccgcc
7621 atgaggtgac agagatggga caagaagtga ctctgagatg taaaccaatt tcaggccaca
7681 actccctttt ctggtacaga cagaccatga tgcggggact ggagttgctc atttacttta
7741 acaacaacgt tccgatagat gattcaggga tgcccgagga tcgattctca gctaagatgc
7801 ctaatgcatc attctccact ctgaagatcc agccctcaga acccagggac tcagctgtgt
7861 acttctgtgc cagcagtttc tcgacctgtt cggctaacta tggctacacc ttcggttcgg
7921 ggaccaggtt aaccgttgta gaggacctga acaaggtgtt cccacccgag gtcgctgtgt
7981 ttgagccatc agaagcagaa atctcccaca cccaaaaggc cacactggtg tgcctggcca
8041 caggcttctt ccccgaccac gtggagctga gctggtgggt gaatgggaag gaggtgcaca
8101 gtggggtcag cacagacccg cagcccctca aggagcagcc cgccctcaat gactccagat
8161 actgcctgag cagccgcctg agggtctcgg ccaccttctg gcagaacccc cgcaaccact
8221 tccgctgtca agtccagttc tacgggctct cggagaatga cgagtggacc caggataggg
8281 ccaaacccgt cacccagatc gtcagcgccg aggcctgggg tagagcagac tgtggcttta
8341 cctcggtgtc ctaccagcaa ggggtcctgt ctgccaccat cctctatgag atcctgctag
8401 ggaaggccac cctgtatgct gtgctggtca gcgcccttgt gttgatggcc atggtcaaga
8461 gaaaggattt cacgcgtggc tccggcgcca cgaacttctc tctgttaaag caagcaggag
8521 acgtggaaga aaaccccggt cccggtacca tgcagtcggg cactcactgg agagttctgg
8581 gcctctgcct cttatcagtt ggcgtttggg ggcaagatgg taatgaagaa atgggtggta
8641 ttacacagac accatataaa gtctccatct ctggaaccac agtaatattg acatgccctc
8701 agtatcctgg atctgaaata ctatggcaac acaatgataa aaacataggc ggtgatgagg
8761 atgataaaaa cataggcagt gatgaggatc acctgtcact gaaggaattt tcagaattgg
8821 agcaaagtgg ttattatgtc tgctacccca gaggaagcaa accagaagat gcgaactttt
8881 atctctacct gagggcaaga gtgtgtgaga actgcatgga gatggatgtg atgtcggtgg
8941 ccacaattgt catagtggac atctgcatca ctgggggctt gctgctgctg gtttactact
9001 ggagcaagaa tagaaaggcc aaggccaagc ctgtgacacg aggagcgggt gctggcggca
9061 ggcaaagggg acaaaacaag gagaggccac cacctgttcc caacccagac tatgagccca
9121 tccggaaagg ccagcgggac ctgtattctg gcctgaatca gagacgcatc ggatccgggt
9181 ccggcgaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat cctggcccaa
9241 ccggtatgaa gtggaaggcg cttttcaccg cggccatcct gcaggcacag ttgccgatta
9301 cagaggcaca gagctttggc ctgctggacc ccaaactctg ctacctgctg gatggaatcc
9361 tcttcatcta tggtgtcatt ctcactgcct tgttcctgag agtgaagttc agcaggagcg
9421 cagacgcccc cgcgtaccag cagggccaga accagctcta taacgagctc aatctaggac
9481 gaagagagga gtacgatgtt ttggacaaga gacgtggccg ggaccctgag atggggggaa
9541 agccgcagag aaggaagaac cctcaggaag gcctgtacaa tgaactgcag aaagataaga
9601 tggcggaggc ctacagtgag attgggatga aaggcgagcg ccggaggggc aaggggcacg
9661 atggccttta ccagggtctc agtacagcca ccaaggacac ctacgacgcc cttcacatgc
9721 aggccctgcc ccctcgctga gcggccgcga ctctagagtc gacctgcagg catgcaagct
9781 tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
9841 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
9901 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
9961 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
10021 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
10081 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
10141 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
10201 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
10261 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
10321 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
10381 ctccccgcat cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac
10441 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
10501 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
10561 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
10621 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
10681 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga
10741 attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat
10801 gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg
10861 cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg
10921 acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat
10981 cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg
11041 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
11101 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
11161 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
11221 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
11281 aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
11341 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
11401 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
11461 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
11521 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
11581 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
11641 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
11701 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
11761 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
11821 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
11881 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
11941 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
12001 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
12061 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
12121 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
12181 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
12241 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
12301 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
12361 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
12421 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
12481 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
12541 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
12601 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
12661 gagcgtcaga ccccgtagaa a
//
