| 质粒名称: | pDL278 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 46882 |
| 存储实验室: | Gary Dunny |
|
载体骨架基本信息 |
|
| 载体名称: | pDL276 |
| 载体出品公司: | Addgene plasmid 46881 |
| 载体抗性: | 大观霉素 |
| 空载体大小: | 6733 bp |
| 完整质粒大小: | -- |
| 载体修饰: | 编码aphA3基因和pDL276的pVA380-1 ORF-1的卡那霉素抗性被编码aad9基因的大观霉素抗性和pDL602的pVA380-1ORF-1替换（详细的构造信息见相关文章）。 |
| 载体类型: | 细菌表达；大肠杆菌/革兰氏阳性菌穿梭载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | M13_pUC_rev |
| 3' 测序引物 | M13_pUC_fwd; pBAD_rev |
|
文献引用方法 |
|
| 材料和方法部分: | pDL278 was a gift from Gary Dunny (Addgene plasmid # 46882 ; http://n2t.net/addgene:46882 ; RRID:Addgene_46882) |
| 参考文献部分: | Molecular, genetic, and functional analysis of the basic replicon of pVA380-1, a plasmid of oral streptococcal origin. LeBlanc DJ, Lee LN, Abu-Al-Jaibat A. Plasmid. 1992 Sep;28(2):130-45. 10.1016/0147-619X(92)90044-B PubMed 1409970 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pDL278 6733 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION E. Coli/Gram-positive Bacterial Shuttle Vector.
ACCESSION .
VERSION .
KEYWORDS pDL278
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6733)
AUTHORS LeBlanc DJ, Lee LN, Abu-Al-Jaibat A
TITLE Molecular, genetic, and functional analysis of the basic replicon of
pVA380-1, a plasmid of oral streptococcal origin.
JOURNAL Plasmid. 1992 Sep;28(2):130-45.
PUBMED 1409970
REFERENCE 2 (bases 1 to 6733)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6733
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(66..83)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(237..825)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(317..336)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
terminator 5155..5182
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 5274..5360
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 5653..5680
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 5772..5858
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(6024..6422)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGECRELFTTPRIGANQFLRRTVNAQTNPWQ
NISIASAISSSRTRRISAVLADERRFSA"
primer_bind 6317..6339
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 6331..6348
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 6332..6348
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 6349..6405
/label=MCS
/note="pUC18/19 multiple cloning site"
primer_bind complement(6418..6434)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6418..6434)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6431..6453)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6442..6458
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6466..6496)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6511..6532
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
ORIGIN
1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
421 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
661 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
1021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
1081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
1141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
1201 agaagtggtc cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt
1261 atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct
1321 accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata
1381 ctgataagat aatatatctt ttatatagaa gatcgatttt cgttcgtgaa tacatgttat
1441 aataactata actaataacg taacgtgact ggcaagagat atttttaaaa caatgaatag
1501 gtttacactt actttagttt tatggaaatg aaagatcata tcatatataa tctagaataa
1561 aattaactaa aataattatt atctagataa aaaatttaga agccaatgaa atctataaat
1621 aaactaaatt aagtttattt aattaacaac tatggatata aaataggtac taatcaaaat
1681 agtgaggagg atatatttga atacatacga acaaattaat aaagtgaaaa aaatacttcg
1741 gaaacattta aaaaataacc ttattggtac ttacatgttt ggatcaggag ttgagagtgg
1801 actaaaacca aatagtgatc ttgacttttt agtcgtcgta tctgaaccat tgacagatca
1861 aagtaaagaa atacttatac aaaaaattag acctatttca aaaaaaatag gagataaaag
1921 caacttacga tatattgaat taacaattat tattcagcaa gaaatggtac cgtggaatca
1981 tcctcccaaa caagaattta tttatggaga atggttacaa gagctttatg aacaaggata
2041 cattcctcag aaggaattaa attcagattt aaccataatg ctttaccaag caaaacgaaa
2101 aaataaaaga atatacggaa attatgactt agaggaatta ctacctgata ttccattttc
2161 tgatgtgaga agagccatta tggattcgtc agaggaatta atagataatt atcaggatga
2221 tgaaaccaac tctatattaa ctttatgccg tatgatttta actatggaca cgggtaaaat
2281 cataccaaaa gatattgcgg gaaatgcagt ggctgaatct tctccattag aacataggga
2341 gagaattttg ttagcagttc gtagttatct tggagagaat attgaatgga ctaatgaaaa
2401 tgtaaattta actataaact atttaaataa cagattaaaa aaattataaa aaaattgaaa
2461 aaatggtgga aacacttttt tcaatttttt tgttttatta tttaatattt gggaaatatt
2521 cattctaatt ggtaatcaga ttttagaaaa caataaaccc ttgcataact ttctcgtcca
2581 tatcggaaac tactttttta ttcccttttt ttcgaccgag attattttgc gataaaatcc
2641 gattaagata ctgcctacta actcccaatt cttgagctaa ttctgatact gttttactca
2701 tcagctacct cacaaaataa ttctttctat cccaattcca aaaggcaaca atttcacgac
2761 cagtactttc tgcagattct tctgctccgg tctgaacaag attcccttct tcgacatcat
2821 caagagctaa ctccgccttg attttcttga acaactttcc aaaaccaatc tgcctttttc
2881 ggtgcaaacc attcgttaaa tcttctgtta tccgttcttt gaccatttca gagaataatg
2941 tcacatcttc tgtatctaca tcaaaaggct taacaggata tttagctgtt tcgataatag
3001 ctgacttcaa gtttttacgt ttatgagctt tgacggttcg aatatcaact acaggtgtat
3061 aatccaattt catcgcctta gcccatagtt tcgtccattc ttcttggcta atgtaatttg
3121 tattatttcc agtaaaatag ctagatttca caaataacaa tacatgcaaa tgagggtgat
3181 aattctcatg ctcagttgaa taagttacct cagtggctcg caaatagcca ataacatttt
3241 tatcgacttt cttgtatttc ataagacgat taaaagctcg ccctatttct gaaattgatt
3301 tatttaattt ttcaccacta atgttcttaa tcgtcaaggt caaaaagaga aagcgacctt
3361 ttggatagcg tttcattgct tcatttacca ctaattcagc ttgataagca tacttcattg
3421 accgtctcca gttacaaatt gggcagagct tatttttgca aaaataagac tgatagagtt
3481 ttttcgtgcc gtcttgttgt tcgataaact tcaacacttc ggcacattga tagacccgtt
3541 caaacgaacg atatcctaaa cgctctaaac gaccagcaag ctcaatattt tttaacttgc
3601 gttctcgcca ctttctatct ttgccattct tagaatagtc cttaaagact tgattttcag
3661 tacctgtcat gttataattt tcttgcaaaa aaattttttt gagtatataa aaaagttcct
3721 gcaattctcc taagtttatt attttaaatt ggacacttaa attataaact ttattttatt
3781 aaattgcaag ggcttttttg ttacctaaaa acccagcaat atcaagggtt tgaggtgctt
3841 taaaacaaga aaataaaaaa actcattatt tctatatgta tcaagataag aaaaaaccct
3901 tgctacgcaa ttgggttttt aagggctttt cagccctctt tttcgtttca cgaaaaattc
3961 acccctaaaa cctcaaattt tgactttcta aatccaaacg tggtataata attttaacgc
4021 tagaagttct aggaaacgaa tagcacaacg caaaaaaaac agtagctgga actactgtct
4081 ttcttttttg tcctcaaaaa gaactcaggt taatttttgt cgcgtttgtt tcgaacatac
4141 cgaatcaacc aatcagcact tacctctgca gcaacgccaa ccacaaaagg tatcaaaaca
4201 agttctagga aagtcttcac agcgctcacc tccaatctaa gttcgtaact tagcgaaaga
4261 acgttgcgac ctagctatta taccatgtcc gcttacttta ttccatggtc taattgacaa
4321 caagtaacca agggcggacg tcctttgtcc gtgtcggctc gaaacgctaa agcctttcgg
4381 ctcgtcacgt cctagcgtac tttgcccttg cttattgtca attagctttc acggcataaa
4441 tcgctcaaag gcctagccct ttttcaatca ctcgtttaac tacccttacg attggctgaa
4501 tagctcttaa ctctgatata aaatttttta aggctttatc tggcataaac tctttcagtt
4561 ttcctagcat tttgtcattt tgctgtctca aaactgagtt ttcacttcta agcgctctat
4621 tttcgttcct aagacgctct atgctctctc ccttactttt ttctttagct cgttccaact
4681 cgcttcaaac cgtttagagc gtctcagagg cactatccgc cttaattgct cattctctag
4741 ctctttttga tgaacaaggt ttctgcttcg gtatatcagc cctttaaatt cttcaaactg
4801 ctctttagtc aattccacat attctttacc gaacacgcct tttttagggg taatagccgc
4861 tagttctttc tcagacatga tttttaggtc agctatttgt gtttttagtt tttctaattt
4921 cagcccctcg cgctctaaac gctctatttt cgcttctaag ctcttcgaat aattttctaa
4981 ggtatttata tatccctcag ctttagatag ctctgagagc ctctcagagg tctcagaatc
5041 gccaggaccc aacgctgccc gactgctttc ctgatgcaaa aacgaggcta gtttaccgta
5101 tctgtggggg gatggcttgt agatatgacg acaggaagag tttgtagaaa cgcaaaaagg
5161 ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg cagtttatgg cgggcgtcct
5221 gcccgccacc ctccgggccg ttgcttcgca acgttcaaat ccgctcccgg cggatttgtc
5281 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
5341 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcatggg gagaccccac
5401 actaccatcg gcgctacggc gtttcacttc tgagttcggc atggggtcag gtgggaccac
5461 cgcgctactg ccgccaggca aattctgttt tatcagaccg cttctgcgtt ctgatttaat
5521 ctgtatcagg ctgaaaatct tctctcatcc gccaaaacag ctgctttcct gatgcaaaaa
5581 cgaggctagt ttaccgtatc tgtgggggga tggcttgtag atatgacgac aggaagagtt
5641 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttaatttg atgcctggca
5701 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcgcaac gttcaaatcc
5761 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
5821 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggcagt tccctactct
5881 cgcatgggga gaccccacac taccatcggc gctacggcgt ttcacttctg agttcggcat
5941 ggggtcaggt gggaccaccg cgctactgcc gccaggcaaa ttctgtttta tcagaccgct
6001 tctgcgttct gatttaatct gtatcaggct gaaaatcttc tctcatccgc caaaacagcc
6061 gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg
6121 ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg
6181 aataattccc ggcattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
6241 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
6301 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgagctc
6361 ggtacccggg gatcctctag agtcgacctg caggcatgca agcttggcgt aatcatggtc
6421 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
6481 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
6541 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
6601 ccaacgcgcg gggagaggcg gtttgcgtat tggagcttgt tgtaactgaa aaaggaaaat
6661 tattgtgcca ggcagttgaa agtcagcacc ttttaacgag tgctgaaatg acggctaaat
6721 gggaaacgta ttt
//
