| 质粒名称: | EGFR-GFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 32751 |
| 存储实验室: | Alexander Sorkin |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-N1 |
| 载体出品公司: | Clontech |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4672 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | EGFR |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 3639 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | EGFP（主干上的C端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XbaI(在克隆过程中销毁) |
| 3' 克隆位点: | SacII(未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | EGFP-N |
|
文献引用方法 |
|
| 材料和方法部分: | EGFR-GFP was a gift from Alexander Sorkin (Addgene plasmid # 32751 ; http://n2t.net/addgene:32751 ; RRID:Addgene_32751) |
| 参考文献部分: | Endocytosis of functional epidermal growth factor receptor-green fluorescent protein chimera. Carter RE, Sorkin A. J Biol Chem. 1998 Dec 25;273(52):35000-7. 10.1074/jbc.273.52.35000 PubMed 9857032 |
买家导航
EGFRGFP载体基本信息
EGFRGFP载体图谱质粒图谱和多克隆位点信息
EGFRGFP载体简介
EGFRGFP载体序列
LOCUS EGFR-GFP 8432 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Mammalian expression of epidermal growth factor receptor (EGFR)
fused to EGFP .
ACCESSION .
VERSION .
KEYWORDS EGFR-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8432)
AUTHORS Carter RE, Sorkin A
TITLE Endocytosis of functional epidermal growth factor receptor-green
fluorescent protein chimera.
JOURNAL J Biol Chem. 1998 Dec 25;273(52):35000-7.
PUBMED 9857032
REFERENCE 2 (bases 1 to 8432)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8432
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 68..371
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 372..575
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 526..546
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 725..4354
/codon_start=1
/product="precursor of human epidermal growth factor
receptor"
/label=EGFR
/translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
regulatory 4379..4388
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4385..5104
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4430..4451)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4691..4710)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5038..5059
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 5227..5348
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5264..5283)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5318..5337
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(5355..5810)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5492..5513)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 5704..5723
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 5837..5941
/gene="bla"
/label=AmpR promoter
promoter 5943..6300
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(5966..5986)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 6151..6286
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6213..6232
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 6335..7129
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6389..6408)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 6999..7018
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(7317..7336)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 7361..7408
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 7737..8325
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8226..8245
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
601 agagagctta tcgattctag ccgagtcccc gcctcgccgc caacgccaca accaccgcgc
661 acggccccct gactccgtcc agtattgatc gggagagccg gagcgagctc ttcggggagc
721 agcgatgcga ccctccggga cggccggggc agcgctcctg gcgctgctgg ctgcgctctg
781 cccggcgagt cgggctctgg aggaaaagaa agtttgccaa ggcacgagta acaagctcac
841 gcagttgggc acttttgaag atcattttct cagcctccag aggatgttca ataactgtga
901 ggtggtcctt gggaatttgg aaattaccta tgtgcagagg aattatgatc tttccttctt
961 aaagaccatc caggaggtgg ctggttatgt cctcattgcc ctcaacacag tggagcgaat
1021 tcctttggaa aacctgcaga tcatcagagg aaatatgtac tacgaaaatt cctatgcctt
1081 agcagtctta tctaactatg atgcaaataa aaccggactg aaggagctgc ccatgagaaa
1141 tttacaggaa atcctgcatg gcgccgtgcg gttcagcaac aaccctgccc tgtgcaacgt
1201 ggagagcatc cagtggcggg acatagtcag cagtgacttt ctcagcaaca tgtcgatgga
1261 cttccagaac cacctgggca gctgccaaaa gtgtgatcca agctgtccca atgggagctg
1321 ctggggtgca ggagaggaga actgccagaa actgaccaaa atcatctgtg cccagcagtg
1381 ctccgggcgc tgccgtggca agtcccccag tgactgctgc cacaaccagt gtgctgcagg
1441 ctgcacaggc ccccgggaga gcgactgcct ggtctgccgc aaattccgag acgaagccac
1501 gtgcaaggac acctgccccc cactcatgct ctacaacccc accacgtacc agatggatgt
1561 gaaccccgag ggcaaataca gctttggtgc cacctgcgtg aagaagtgtc cccgtaatta
1621 tgtggtgaca gatcacggct cgtgcgtccg agcctgtggg gccgacagct atgagatgga
1681 ggaagacggc gtccgcaagt gtaagaagtg cgaagggcct tgccgcaaag tgtgtaacgg
1741 aataggtatt ggtgaattta aagactcact ctccataaat gctacgaata ttaaacactt
1801 caaaaactgc acctccatca gtggcgatct ccacatcctg ccggtggcat ttaggggtga
1861 ctccttcaca catactcctc ctctggatcc acaggaactg gatattctga aaaccgtaaa
1921 ggaaatcaca gggtttttgc tgattcaggc ttggcctgaa aacaggacgg acctccatgc
1981 ctttgagaac ctagaaatca tacgcggcag gaccaagcaa catggtcagt tttctcttgc
2041 agtcgtcagc ctgaacataa catccttggg attacgctcc ctcaaggaga taagtgatgg
2101 agatgtgata atttcaggaa acaaaaattt gtgctatgca aatacaataa actggaaaaa
2161 actgtttggg acctccggtc agaaaaccaa aattataagc aacagaggtg aaaacagctg
2221 caaggccaca ggccaggtct gccatgcctt gtgctccccc gagggctgct ggggcccgga
2281 gcccagggac tgcgtctctt gccggaatgt cagccgaggc agggaatgcg tggacaagtg
2341 caaccttctg gagggtgagc caagggagtt tgtggagaac tctgagtgca tacagtgcca
2401 cccagagtgc ctgcctcagg ccatgaacat cacctgcaca ggacggggac cagacaactg
2461 tatccagtgt gcccactaca ttgacggccc ccactgcgtc aagacctgcc cggcaggagt
2521 catgggagaa aacaacaccc tggtctggaa gtacgcagac gccggccatg tgtgccacct
2581 gtgccatcca aactgcacct acggatgcac tgggccaggt cttgaaggct gtccaacgaa
2641 tgggcctaag atcccgtcca tcgccactgg gatggtgggg gccctcctct tgctgctggt
2701 ggtggccctg gggatcggcc tcttcatgcg aaggcgccac atcgttcgga agcgcacgct
2761 gcggaggctg ctgcaggaga gggagcttgt ggagcctctt acacccagtg gagaagctcc
2821 caaccaagct ctcttgagga tcttgaagga aactgaattc aaaaagatca aagtgctggg
2881 ctccggtgcg ttcggcacgg tgtataaggg actctggatc ccagaaggtg agaaagttaa
2941 aattcccgtc gctatcaagg aattaagaga agcaacatct ccgaaagcca acaaggaaat
3001 cctcgatgaa gcctacgtga tggccagcgt ggacaacccc cacgtgtgcc gcctgctggg
3061 catctgcctc acctccaccg tgcaactcat cacgcagctc atgcccttcg gctgcctcct
3121 ggactatgtc cgggaacaca aagacaatat tggctcccag tacctgctca actggtgtgt
3181 gcagatcgca aagggcatga actacttgga ggaccgtcgc ttggtgcacc gcgacctggc
3241 agccaggaac gtactggtga aaacaccgca gcatgtcaag atcacagatt ttgggctggc
3301 caaactgctg ggtgcggaag agaaagaata ccatgcagaa ggaggcaaag tgcctatcaa
3361 gtggatggca ttggaatcaa ttttacacag aatctatacc caccagagtg atgtctggag
3421 ctacggggtg accgtttggg agttgatgac ctttggatcc aagccatatg acggaatccc
3481 tgccagcgag atctcctcca tcctggagaa aggagaacgc ctccctcagc cacccatatg
3541 taccatcgat gtctacatga tcatggtcaa gtgctggatg atagacgcag atagtcgccc
3601 aaagttccgt gagttgatca tcgaattctc caaaatggcc cgagaccccc agcgctacct
3661 tgtcattcag ggggatgaaa gaatgcattt gccaagtcct acagactcca acttctaccg
3721 tgccctgatg gatgaagaag acatggacga cgtggtggat gccgacgagt acctcatccc
3781 acagcagggc ttcttcagca gcccctccac gtcacggact cccctcctga gctctctgag
3841 tgcaaccagc aacaattcca ccgtggcttg cattgataga aatgggctgc aaagctgtcc
3901 catcaaggaa gacagcttct tgcagcgata cagctcagac cccacaggcg ccttgactga
3961 ggacagcata gacgacacct tcctcccagt gcctgaatac ataaaccagt ccgttcccaa
4021 aaggcccgct ggctctgtgc agaatcctgt ctatcacaat cagcctctga accccgcgcc
4081 cagcagagac ccacactacc aggaccccca cagcactgca gtgggcaacc ccgagtatct
4141 caacactgtc cagcccacct gtgtcaacag cacattcgac agccctgccc actgggccca
4201 gaaaggcagc caccaaatta gcctggacaa ccctgactac cagcaggact tctttcccaa
4261 ggaagccaag ccaaatggca tctttaaggg ctccacagct gaaaatgcag aatacctaag
4321 ggtcgcgcca caaagcagtg aatttattgg agcaccgcgg gcccgggatc caccggtcgc
4381 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
4441 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
4501 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
4561 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
4621 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
4681 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
4741 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
4801 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
4861 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
4921 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
4981 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
5041 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
5101 gtaaagcggc cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt
5161 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
5221 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
5281 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
5341 gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
5401 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
5461 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
5521 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
5581 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
5641 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
5701 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
5761 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
5821 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
5881 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
5941 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
6001 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
6061 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
6121 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
6181 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
6241 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
6301 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
6361 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
6421 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
6481 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
6541 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
6601 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
6661 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
6721 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
6781 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
6841 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
6901 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
6961 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
7021 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
7081 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
7141 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
7201 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
7261 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
7321 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
7381 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
7441 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
7501 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
7561 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt
7621 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
7681 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
7741 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
7801 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
7861 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
7921 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
7981 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
8041 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
8101 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
8161 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
8221 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
8281 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
8341 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
8401 tcccctgatt ctgtggataa ccgtattacc gc
//
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EGFRGFP载体质粒应用举例
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