| 质粒名称: | pRW006-SM-destination-pro35S-Cas9 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 104439 |
| 存储实验室: | Detlef Weigel |
|
载体骨架基本信息 |
|
| 载体名称: | pZ001 |
| 载体出品公司: | Lampropoulos et al |
| 载体抗性: | 大观霉素 |
| 空载体大小: | 4114 bp |
| 完整质粒大小: | -- |
| 载体修饰: | 将pro:：pcoCas9和At2S3：：mcherry的文盒设计成主干；用proLacZ:：LacZ表达单元代替ccdB氯霉素盒 |
| 载体类型: | 细菌表达，植物表达，CRISPR |
| 筛选标记: | red fluorescence |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | 35S |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pRW006-SM-destination-pro35S-Cas9 was a gift from Detlef Weigel (Addgene plasmid # 104439 ; http://n2t.net/addgene:104439 ; RRID:Addgene_104439) |
| 参考文献部分: | An efficient CRISPR vector toolbox for engineering large deletions in Arabidopsis thaliana. Wu R, Lucke M, Jang YT, Zhu W, Symeonidi E, Wang C, Fitz J, Xi W, Schwab R, Weigel D. Plant Methods. 2018 Aug 2;14:65. doi: 10.1186/s13007-018-0330-7. eCollection 2018. 10.1186/s13007-018-0330-7 PubMed 30083222 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pRW006-SM-destination-pro35S-Cas9 11115 bp ds-DNA circular SYN 05-SEP-2018
DEFINITION Destination vector expressing plant-codon-optimized Cas9 under 35S
promoter, with sgRNAs be shuffled in; seed coat specific red
fluorescence for screening trangene free.
ACCESSION .
VERSION .
KEYWORDS pRW006-SM-destination-pro35S-Cas9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11115)
TITLE Super Module CRISPR/Cas9 vector tool box for genome editing in
Arabidopsis
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 11115)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 5, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11115
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 534..556
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA
(truncated)"
promoter 1086..1431
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind 1374..1394
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
CDS 1460..1507
/codon_start=1
/product="two tandem FLAG(R) epitope tags"
/label=2xFLAG
/translation="DYKDDDDKDYKDDDDK"
CDS 1514..1534
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS join(1559..2995,3185..5848)
/codon_start=1
/product="Cas9 endonuclease from the Streptococcus pyogenes
Type II CRISPR/Cas system"
/label=pcoCas9
/note="plant codon-optimized Cas9 gene containing the
potato IV2 intron"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
intron 2996..3184
/label=IV2 intron
/note="modified second intron of the potato ST-LS1 gene
(Vancanneyt et al., 1990)"
CDS 5849..5896
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
terminator 5902..6543
/label=E9 terminator
/note="terminator and polyadenylation signal from the pea
rbcS-E9 gene"
CDS complement(6766..7089)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITPSLHACRSTLEDPCVPSSNSLAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
DAA"
primer_bind 6998..7015
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 6999..7015
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7085..7101)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7085..7101)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7098..7120)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7109..7125
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7133..7163)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7178..7199
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7316..7333)
/label=L4440
/note="L4440 vector, forward primer"
terminator 7712..7964
/label=MAS terminator
/note="mannopine synthase terminator"
primer_bind complement(8248..8267)
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind 8517..8535
/label=mCherry-R
/note="mCherry, reverse primer"
misc_feature 9134..9158
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin complement(9249..9837)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9329..9348)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10008..10796)
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 11087..407
/label=pSa ori
/note="origin of replication from bacterial plasmid pSa"
ORIGIN
1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
541 atatattgtg gtgtaacgtt ctcgaactag gctcggacga agtaagcatt cggtccccag
601 attagccttt tcaatttcag aaagaatgct aacccacaga tggttagaga ggcttacgca
661 gcacgtctca tcaagacgat ctacccgagc aataatctcc aggaaatcaa ataccttccc
721 aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa cacagagaaa
781 gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcac
841 aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcccac tgaatcaaag
901 gccatggagt caaagattca aatagaggac ctaacagaac tcgccgtaaa gactggcgaa
961 cagttcatac agagtctctt acgactcaat gacaagaaga aaatcttcgt caacatggtg
1021 gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg
1081 gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca
1141 gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat
1201 cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat
1261 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
1321 caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct
1381 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagaac acgggggacg
1441 agctaacagc ggatccatgg attacaagga tgatgatgat aaggattaca aggatgatga
1501 tgataagatg gctccaaaga agaagagaaa ggttggaatc cacggagttc cagctgctga
1561 taagaagtac tctatcggac ttgacatcgg aaccaactct gttggatggg ctgttatcac
1621 cgatgagtac aaggttccat ctaagaagtt caaggttctt ggaaacaccg atagacactc
1681 tatcaagaag aaccttatcg gtgctcttct tttcgattct ggagaaaccg ctgaggctac
1741 cagattgaag agaaccgcta gaagaagata caccagaaga aagaacagaa tctgctacct
1801 tcaggaaatc ttctctaacg agatggctaa ggttgatgat tctttcttcc acagacttga
1861 ggagtctttc cttgttgagg aggataagaa gcacgagaga cacccaatct tcggaaacat
1921 cgttgatgag gttgcttacc acgagaagta cccaaccatc taccacctta gaaagaagtt
1981 ggttgattct accgataagg ctgatcttag acttatctac cttgctcttg ctcacatgat
2041 caagttcaga ggacacttcc ttatcgaggg tgaccttaac ccagataact ctgatgttga
2101 taagttgttc atccagcttg ttcagaccta caaccagctt ttcgaggaga acccaatcaa
2161 cgcttctgga gttgatgcta aggctatcct ttctgctaga ctttctaagt ctcgtagact
2221 tgagaacctt atcgctcagc ttccaggaga gaagaagaac ggacttttcg gaaaccttat
2281 cgctctttct cttggactta ccccaaactt caagtctaac ttcgatcttg ctgaggatgc
2341 taagttgcag ctttctaagg atacctacga tgatgatctt gataaccttc ttgctcagat
2401 cggagatcag tacgctgatc ttttccttgc tgctaagaac ctttctgatg ctatccttct
2461 ttctgacatc cttagagtta acaccgagat caccaaggct ccactttctg cttctatgat
2521 caagagatac gatgagcacc accaggatct tacccttttg aaggctcttg ttagacagca
2581 gcttccagag aagtacaagg aaatcttctt cgatcagtct aagaacggat acgctggata
2641 catcgatgga ggagcttctc aggaggagtt ctacaagttc atcaagccaa tccttgagaa
2701 gatggatgga accgaggagc ttcttgttaa gttgaacaga gaggatcttc ttagaaagca
2761 gagaaccttc gataacggat ctatcccaca ccagatccac cttggagagc ttcacgctat
2821 ccttcgtaga caggaggatt tctacccatt cttgaaggat aacagagaga agatcgagaa
2881 gatccttacc ttcagaatcc catactacgt tggaccactt gctagaggaa actctcgttt
2941 cgcttggatg accagaaagt ctgaggaaac catcacccct tggaacttcg aggaggtaag
3001 tttctgcttc tacctttgat atatatataa taattatcat taattagtag taatataata
3061 tttcaaatat ttttttcaaa ataaaagaat gtagtatata gcaattgctt ttctgtagtt
3121 tataagtgtg tatattttaa tttataactt ttctaatata tgaccaaaat ttgttgatgt
3181 gcaggttgtt gataagggag cttctgctca gtctttcatc gagagaatga ccaacttcga
3241 taagaacctt ccaaacgaga aggttcttcc aaagcactct cttctttacg agtacttcac
3301 cgtttacaac gagcttacca aggttaagta cgttaccgag ggaatgagaa agccagcttt
3361 cctttctgga gagcagaaga aggctatcgt tgatcttctt ttcaagacca acagaaaggt
3421 taccgttaag cagttgaagg aggattactt caagaagatc gagtgcttcg attctgttga
3481 aatctctgga gttgaggata gattcaacgc ttctcttgga acctaccacg atcttttgaa
3541 gatcatcaag gataaggatt tccttgataa cgaggagaac gaggacatcc ttgaggacat
3601 cgttcttacc cttacccttt tcgaggatag agagatgatc gaggagagac tcaagaccta
3661 cgctcacctt ttcgatgata aggttatgaa gcagttgaag agaagaagat acaccggatg
3721 gggtagactt tctcgtaagt tgatcaacgg aatcagagat aagcagtctg gaaagaccat
3781 ccttgatttc ttgaagtctg atggattcgc taacagaaac ttcatgcagc ttatccacga
3841 tgattctctt accttcaagg aggacatcca gaaggctcag gtttctggac agggagattc
3901 tcttcacgag cacatcgcta accttgctgg atctccagct atcaagaagg gaatccttca
3961 gaccgttaag gttgttgatg agcttgttaa ggttatgggt agacacaagc cagagaacat
4021 cgttatcgag atggctagag agaaccagac cacccagaag ggacagaaga actctcgtga
4081 gagaatgaag agaatcgagg agggaatcaa ggagcttgga tctcaaatct tgaaggagca
4141 cccagttgag aacacccagc ttcagaacga gaagttgtac ctttactacc ttcagaacgg
4201 aagagatatg tacgttgatc aggagcttga catcaacaga ctttctgatt acgatgttga
4261 tcacatcgtt ccacagtctt tcttgaagga tgattctatc gataacaagg ttcttacccg
4321 ttctgataag aacagaggaa agtctgataa cgttccatct gaggaggttg ttaagaagat
4381 gaagaactac tggagacagc ttcttaacgc taagttgatc acccagagaa agttcgataa
4441 ccttaccaag gctgagagag gaggactttc tgagcttgat aaggctggat tcatcaagag
4501 acagcttgtt gaaaccagac agatcaccaa gcacgttgct cagatccttg attctcgtat
4561 gaacaccaag tacgatgaga acgataagtt gatcagagag gttaaggtta tcaccttgaa
4621 gtctaagttg gtttctgatt tcagaaagga tttccagttc tacaaggtta gagagatcaa
4681 caactaccac cacgctcacg atgcttacct taacgctgtt gttggaaccg ctcttatcaa
4741 gaagtaccca aagttggagt ctgagttcgt ttacggagat tacaaggttt acgatgttag
4801 aaagatgatc gctaagtctg agcaggagat cggaaaggct accgctaagt acttcttcta
4861 ctctaacatc atgaacttct tcaagaccga gatcaccctt gctaacggag agatcagaaa
4921 gaggccactt atcgaaacca acggagaaac cggagagatc gtttgggata agggaagaga
4981 tttcgctacc gttagaaagg ttctttctat gccacaggtt aacatcgtta agaaaaccga
5041 ggttcagacc ggaggattct ctaaggagtc tatccttcca aagagaaact ctgataagtt
5101 gatcgctaga aagaaggatt gggacccaaa gaagtacgga ggattcgatt ctccaaccgt
5161 tgcttactct gttcttgttg ttgctaaggt tgagaaggga aagtctaaga agttgaagtc
5221 tgttaaggag cttcttggaa tcaccatcat ggagcgttct tctttcgaga agaacccaat
5281 cgatttcctt gaggctaagg gatacaagga ggttaagaag gatcttatca tcaagttgcc
5341 aaagtactct cttttcgagc ttgagaacgg aagaaagaga atgcttgctt ctgctggaga
5401 gcttcagaag ggaaacgagc ttgctcttcc atctaagtac gttaacttcc tttaccttgc
5461 ttctcactac gagaagttga agggatctcc agaggataac gagcagaagc agcttttcgt
5521 tgagcagcac aagcactacc ttgatgagat catcgagcaa atctctgagt tctctaagag
5581 agttatcctt gctgatgcta accttgataa ggttctttct gcttacaaca agcacagaga
5641 taagccaatc agagagcagg ctgagaacat catccacctt ttcaccctta ccaaccttgg
5701 tgctccagct gctttcaagt acttcgatac caccatcgat agaaaaagat acacctctac
5761 caaggaggtt cttgatgcta cccttatcca ccagtctatc accggacttt acgagaccag
5821 aatcgatctt tctcagcttg gaggagataa gagaccagct gctaccaaga aggctggaca
5881 ggctaagaag aagaagtgaa aagctttcgt tcgtatcatc ggtttcgaca acgttcgtca
5941 agttcaatgc atcagtttca ttgcgcacac accagaatcc tactgagttt gagtattatg
6001 gcattgggaa aactgttttt cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt
6061 attcggtttt cgctatcgaa ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat
6121 atggtccttt tgttcattct caaattaata ttatttgttt tttctcttat ttgttgtgtg
6181 ttgaatttga aattataaga gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat
6241 cgtggcctct aatgaccgaa gttaatatga ggagtaaaac acttgtagtt gtaccattat
6301 gcttattcac taggcaacaa atatattttc agacctagaa aagctgcaaa tgttactgaa
6361 tacaagtatg tcctcttgtg ttttagacat ttatgaactt tcctttatgt aattttccag
6421 aatccttgtc agattctaat cattgcttta taattatagt tatactcatg gatttgtagt
6481 tgagtatgaa aatatttttt aatgcatttt atgacttgcc aattgattga caacatgcat
6541 caatcggtac cacctagtag aagagctcag tgagaccgaa ttcgcttact aaaagccaga
6601 taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat
6661 acccgaagta tgtcaaaaag aggtgtgcta agcttcggat gccgggagca gacaagcccg
6721 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
6781 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
6841 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
6901 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
6961 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
7021 cgagctcggt acacagggat cttcaagagt cgacctgcag gcatgcaggc ttggcgtaat
7081 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
7141 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
7201 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
7261 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
7321 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcacta gtaggaggta
7381 atcaatgctg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
7441 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
7501 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
7561 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
7621 ctctgatgcc gcatgatcat ctggcggacc tcttcggagg tccgcttttt tttctcgagg
7681 gtctcaacta gctcttcatg ggtattctag agataattta tttgaaaatt cataagaaaa
7741 gcaaacgtta catgaattga tgaaacaata caaagacaga taaagccacg cacatttagg
7801 atattggccg agattactga atattgagta agatcacgga atttctgaca ggagcatgtc
7861 ttcaattcag cccaaatggc agttgaaata ctcaaaccgc cccatatgca ggagcggatc
7921 attcattgtt tgtttggttg cctttgccaa catgggagtc caagagctct cacttgtaca
7981 gctcgtccat gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg
8041 tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt gacgttgtag gcgccgggca
8101 gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag tggccgccgt
8161 ccttcagctt cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc
8221 gctcggagga ggcctcccag cccatggtct tcttctgcat tacggggccg tcggagggga
8281 agttggtgcc gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg gaggagtcct
8341 gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct
8401 cggggaagga cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg taggccttgg
8461 agccgtacat gaactgaggg gacaggatgt cccaggcgaa gggcaggggg ccacccttgg
8521 tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc tcgccctcga
8581 tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc atgaactcct
8641 tgatgatggc catgttatcc attgttcgta ccgttttgct atttgtgtat gttttcttgt
8701 tttggtttga gtgaagagtg tagtgaaggg ttggtttgta tttataggtg agaaaggaga
8761 tttgcatggc aatcacgtgt aagaatgcat gcatgcatga gtgtgtgtgg agagagagag
8821 atgctcttac gttgttgtcg ggattatgtg ttggaaaaga gtgacaagtg acgaacaaaa
8881 caataccact catcatagct ccgcttatgc atgctatgtg tgatttgtag ctcttgttct
8941 gacatgacac tatgaacaat tttttaaaaa gaaggttaaa cttttattta aataaaaacc
9001 ctatgttaat ttggtttcaa tcgagtaaga tgagaagtca atcttttgat ggtagctagt
9061 gatgttggaa atatagttgt gccagcttat ctagaccaag catgcacagc tggcgttctc
9121 agccagatcc ccttgacagg atatattggc gggtaaacta agtcgctgta tgtgtttgtt
9181 tgagatctca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
9241 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
9301 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
9361 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
9421 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
9481 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
9541 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
9601 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
9661 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
9721 ccagttacct tcggaagaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
9781 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
9841 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
9901 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
9961 agttttaaat caatctaaag tatatatgtg taacattggt ctagtgatta tttgccgact
10021 accttggtga tctcgccttt cacgtagtga acaaattctt ccaactgatc tgcgcgcgag
10081 gccaagcgat cttcttgtcc aagataagcc tgcctagctt caagtatgac gggctgatac
10141 tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg
10201 gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc
10261 cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt
10321 tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct
10381 cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct
10441 gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc
10501 cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc
10561 tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca
10621 tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca
10681 tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg
10741 acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcataaca
10801 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta
10861 tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga
10921 acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg
10981 gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt
11041 ggctccctca ctttctggct ggatgatggg gcgattcagg cgatccccat ccaacagccc
11101 gccgtcgagc gggct
//
