| 质粒名称: | pRW004-SM-destination-proUBQ10-Cas9 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 104438 |
| 存储实验室: | Detlef Weigel |
|
载体骨架基本信息 |
|
| 载体名称: | pZ001 |
| 载体出品公司: | Lampropoulos et al |
| 载体抗性: | 大观霉素 |
| 空载体大小: | 4114 bp |
| 完整质粒大小: | -- |
| 载体修饰: | 将pro:：pcoCas9和At2S3：：mcherry的文盒设计成主干；用proLacZ:：LacZ表达单元代替ccdB氯霉素盒 |
| 载体类型: | 细菌表达，植物表达，CRISPR |
| 筛选标记: | red fluorescence |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | UBQ10 |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pRW004-SM-destination-proUBQ10-Cas9 was a gift from Detlef Weigel (Addgene plasmid # 104438 ; http://n2t.net/addgene:104438 ; RRID:Addgene_104438) |
| 参考文献部分: | An efficient CRISPR vector toolbox for engineering large deletions in Arabidopsis thaliana. Wu R, Lucke M, Jang YT, Zhu W, Symeonidi E, Wang C, Fitz J, Xi W, Schwab R, Weigel D. Plant Methods. 2018 Aug 2;14:65. doi: 10.1186/s13007-018-0330-7. eCollection 2018. 10.1186/s13007-018-0330-7 PubMed 30083222 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pRW004-SM-destination-proUBQ10-Cas9 10887 bp ds-DNA circular SYN 07-AUG-2018
DEFINITION Destination vector expressing plant-codon-optimized Cas9 under UBQ10
promoter, with sgRNAs be shuffled in; seed coat specific red
fluorescence for screening trangene free plants;.
ACCESSION .
VERSION .
KEYWORDS pRW004-SM-destination-proUBQ10-Cas9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10887)
TITLE Super Module CRISPR/Cas9 vector tool box for genome editing in
Arabidopsis
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 10887)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 7, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10887
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 534..556
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA
(truncated)"
CDS 1232..1279
/codon_start=1
/product="two tandem FLAG(R) epitope tags"
/label=2xFLAG
/translation="DYKDDDDKDYKDDDDK"
CDS 1286..1306
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS join(1331..2767,2957..5620)
/codon_start=1
/product="Cas9 endonuclease from the Streptococcus pyogenes
Type II CRISPR/Cas system"
/label=pcoCas9
/note="plant codon-optimized Cas9 gene containing the
potato IV2 intron"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
intron 2768..2956
/label=IV2 intron
/note="modified second intron of the potato ST-LS1 gene
(Vancanneyt et al., 1990)"
CDS 5621..5668
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
terminator 5674..6315
/label=E9 terminator
/note="terminator and polyadenylation signal from the pea
rbcS-E9 gene"
CDS complement(6538..6861)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITPSLHACRSTLEDPCVPSSNSLAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
DAA"
primer_bind 6770..6787
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 6771..6787
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6857..6873)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6857..6873)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6870..6892)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6881..6897
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6905..6935)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6950..6971
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7088..7105)
/label=L4440
/note="L4440 vector, forward primer"
terminator 7484..7736
/label=MAS terminator
/note="mannopine synthase terminator"
primer_bind complement(8020..8039)
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind 8289..8307
/label=mCherry-R
/note="mCherry, reverse primer"
misc_feature 8906..8930
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin complement(9021..9609)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9101..9120)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9780..10568)
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 10859..407
/label=pSa ori
/note="origin of replication from bacterial plasmid pSa"
ORIGIN
1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
541 atatattgtg gtgtaacgtt ctcgaactag gctcggacga agtaagccga cgagtcagta
601 ataaacggcg tcaaagtggt tgcagccggc acacacgagt cgtgtttatc aactcaaagc
661 acaaatactt ttcctcaacc taaaaataag gcaattagcc aaaaacaact ttgcgtgtaa
721 acaacgctca atacacgtgt cattttatta ttagctattg cttcaccgcc ttagctttct
781 cgtgacctag tcgtcctcgt cttttcttct tcttcttcta taaaacaata cccaaagagc
841 tcttcttctt cacaattcag atttcaattt ctcaaaatct taaaaacttt ctctcaattc
901 tctctaccgt gatcaaggta aatttctgtg ttccttattc tctcaaaatc ttcgattttg
961 ttttcgttcg atcccaattt cgtatatgtt ctttggttta gattctgtta atcttagatc
1021 gaagacgatt ttctgggttt gatcgttaga tatcatctta attctcgatt agggtttcat
1081 agatatcatc cgatttgttc aaataatttg agttttgtcg aataattact cttcgatttg
1141 tgatttctat ctagatctgg tgttagtttc tagtttgtgc gatcgaattt gtagattaat
1201 ctgagttttt ctgattaaca gcggatccat ggattacaag gatgatgatg ataaggatta
1261 caaggatgat gatgataaga tggctccaaa gaagaagaga aaggttggaa tccacggagt
1321 tccagctgct gataagaagt actctatcgg acttgacatc ggaaccaact ctgttggatg
1381 ggctgttatc accgatgagt acaaggttcc atctaagaag ttcaaggttc ttggaaacac
1441 cgatagacac tctatcaaga agaaccttat cggtgctctt cttttcgatt ctggagaaac
1501 cgctgaggct accagattga agagaaccgc tagaagaaga tacaccagaa gaaagaacag
1561 aatctgctac cttcaggaaa tcttctctaa cgagatggct aaggttgatg attctttctt
1621 ccacagactt gaggagtctt tccttgttga ggaggataag aagcacgaga gacacccaat
1681 cttcggaaac atcgttgatg aggttgctta ccacgagaag tacccaacca tctaccacct
1741 tagaaagaag ttggttgatt ctaccgataa ggctgatctt agacttatct accttgctct
1801 tgctcacatg atcaagttca gaggacactt ccttatcgag ggtgacctta acccagataa
1861 ctctgatgtt gataagttgt tcatccagct tgttcagacc tacaaccagc ttttcgagga
1921 gaacccaatc aacgcttctg gagttgatgc taaggctatc ctttctgcta gactttctaa
1981 gtctcgtaga cttgagaacc ttatcgctca gcttccagga gagaagaaga acggactttt
2041 cggaaacctt atcgctcttt ctcttggact taccccaaac ttcaagtcta acttcgatct
2101 tgctgaggat gctaagttgc agctttctaa ggatacctac gatgatgatc ttgataacct
2161 tcttgctcag atcggagatc agtacgctga tcttttcctt gctgctaaga acctttctga
2221 tgctatcctt ctttctgaca tccttagagt taacaccgag atcaccaagg ctccactttc
2281 tgcttctatg atcaagagat acgatgagca ccaccaggat cttacccttt tgaaggctct
2341 tgttagacag cagcttccag agaagtacaa ggaaatcttc ttcgatcagt ctaagaacgg
2401 atacgctgga tacatcgatg gaggagcttc tcaggaggag ttctacaagt tcatcaagcc
2461 aatccttgag aagatggatg gaaccgagga gcttcttgtt aagttgaaca gagaggatct
2521 tcttagaaag cagagaacct tcgataacgg atctatccca caccagatcc accttggaga
2581 gcttcacgct atccttcgta gacaggagga tttctaccca ttcttgaagg ataacagaga
2641 gaagatcgag aagatcctta ccttcagaat cccatactac gttggaccac ttgctagagg
2701 aaactctcgt ttcgcttgga tgaccagaaa gtctgaggaa accatcaccc cttggaactt
2761 cgaggaggta agtttctgct tctacctttg atatatatat aataattatc attaattagt
2821 agtaatataa tatttcaaat atttttttca aaataaaaga atgtagtata tagcaattgc
2881 ttttctgtag tttataagtg tgtatatttt aatttataac ttttctaata tatgaccaaa
2941 atttgttgat gtgcaggttg ttgataaggg agcttctgct cagtctttca tcgagagaat
3001 gaccaacttc gataagaacc ttccaaacga gaaggttctt ccaaagcact ctcttcttta
3061 cgagtacttc accgtttaca acgagcttac caaggttaag tacgttaccg agggaatgag
3121 aaagccagct ttcctttctg gagagcagaa gaaggctatc gttgatcttc ttttcaagac
3181 caacagaaag gttaccgtta agcagttgaa ggaggattac ttcaagaaga tcgagtgctt
3241 cgattctgtt gaaatctctg gagttgagga tagattcaac gcttctcttg gaacctacca
3301 cgatcttttg aagatcatca aggataagga tttccttgat aacgaggaga acgaggacat
3361 ccttgaggac atcgttctta cccttaccct tttcgaggat agagagatga tcgaggagag
3421 actcaagacc tacgctcacc ttttcgatga taaggttatg aagcagttga agagaagaag
3481 atacaccgga tggggtagac tttctcgtaa gttgatcaac ggaatcagag ataagcagtc
3541 tggaaagacc atccttgatt tcttgaagtc tgatggattc gctaacagaa acttcatgca
3601 gcttatccac gatgattctc ttaccttcaa ggaggacatc cagaaggctc aggtttctgg
3661 acagggagat tctcttcacg agcacatcgc taaccttgct ggatctccag ctatcaagaa
3721 gggaatcctt cagaccgtta aggttgttga tgagcttgtt aaggttatgg gtagacacaa
3781 gccagagaac atcgttatcg agatggctag agagaaccag accacccaga agggacagaa
3841 gaactctcgt gagagaatga agagaatcga ggagggaatc aaggagcttg gatctcaaat
3901 cttgaaggag cacccagttg agaacaccca gcttcagaac gagaagttgt acctttacta
3961 ccttcagaac ggaagagata tgtacgttga tcaggagctt gacatcaaca gactttctga
4021 ttacgatgtt gatcacatcg ttccacagtc tttcttgaag gatgattcta tcgataacaa
4081 ggttcttacc cgttctgata agaacagagg aaagtctgat aacgttccat ctgaggaggt
4141 tgttaagaag atgaagaact actggagaca gcttcttaac gctaagttga tcacccagag
4201 aaagttcgat aaccttacca aggctgagag aggaggactt tctgagcttg ataaggctgg
4261 attcatcaag agacagcttg ttgaaaccag acagatcacc aagcacgttg ctcagatcct
4321 tgattctcgt atgaacacca agtacgatga gaacgataag ttgatcagag aggttaaggt
4381 tatcaccttg aagtctaagt tggtttctga tttcagaaag gatttccagt tctacaaggt
4441 tagagagatc aacaactacc accacgctca cgatgcttac cttaacgctg ttgttggaac
4501 cgctcttatc aagaagtacc caaagttgga gtctgagttc gtttacggag attacaaggt
4561 ttacgatgtt agaaagatga tcgctaagtc tgagcaggag atcggaaagg ctaccgctaa
4621 gtacttcttc tactctaaca tcatgaactt cttcaagacc gagatcaccc ttgctaacgg
4681 agagatcaga aagaggccac ttatcgaaac caacggagaa accggagaga tcgtttggga
4741 taagggaaga gatttcgcta ccgttagaaa ggttctttct atgccacagg ttaacatcgt
4801 taagaaaacc gaggttcaga ccggaggatt ctctaaggag tctatccttc caaagagaaa
4861 ctctgataag ttgatcgcta gaaagaagga ttgggaccca aagaagtacg gaggattcga
4921 ttctccaacc gttgcttact ctgttcttgt tgttgctaag gttgagaagg gaaagtctaa
4981 gaagttgaag tctgttaagg agcttcttgg aatcaccatc atggagcgtt cttctttcga
5041 gaagaaccca atcgatttcc ttgaggctaa gggatacaag gaggttaaga aggatcttat
5101 catcaagttg ccaaagtact ctcttttcga gcttgagaac ggaagaaaga gaatgcttgc
5161 ttctgctgga gagcttcaga agggaaacga gcttgctctt ccatctaagt acgttaactt
5221 cctttacctt gcttctcact acgagaagtt gaagggatct ccagaggata acgagcagaa
5281 gcagcttttc gttgagcagc acaagcacta ccttgatgag atcatcgagc aaatctctga
5341 gttctctaag agagttatcc ttgctgatgc taaccttgat aaggttcttt ctgcttacaa
5401 caagcacaga gataagccaa tcagagagca ggctgagaac atcatccacc ttttcaccct
5461 taccaacctt ggtgctccag ctgctttcaa gtacttcgat accaccatcg atagaaaaag
5521 atacacctct accaaggagg ttcttgatgc tacccttatc caccagtcta tcaccggact
5581 ttacgagacc agaatcgatc tttctcagct tggaggagat aagagaccag ctgctaccaa
5641 gaaggctgga caggctaaga agaagaagtg aaaagctttc gttcgtatca tcggtttcga
5701 caacgttcgt caagttcaat gcatcagttt cattgcgcac acaccagaat cctactgagt
5761 ttgagtatta tggcattggg aaaactgttt ttcttgtacc atttgttgtg cttgtaattt
5821 actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga tggagaagag
5881 ttaatgaatg atatggtcct tttgttcatt ctcaaattaa tattatttgt tttttctctt
5941 atttgttgtg tgttgaattt gaaattataa gagatatgca aacattttgt tttgagtaaa
6001 aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat gaggagtaaa acacttgtag
6061 ttgtaccatt atgcttattc actaggcaac aaatatattt tcagacctag aaaagctgca
6121 aatgttactg aatacaagta tgtcctcttg tgttttagac atttatgaac tttcctttat
6181 gtaattttcc agaatccttg tcagattcta atcattgctt tataattata gttatactca
6241 tggatttgta gttgagtatg aaaatatttt ttaatgcatt ttatgacttg ccaattgatt
6301 gacaacatgc atcaatcggt accacctagt agaagagctc agtgagaccg aattcgctta
6361 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
6421 actgatatgt atacccgaag tatgtcaaaa agaggtgtgc taagcttcgg atgccgggag
6481 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
6541 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
6601 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
6661 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
6721 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
6781 ggccagtgaa ttcgagctcg gtacacaggg atcttcaaga gtcgacctgc aggcatgcag
6841 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
6901 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
6961 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
7021 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
7081 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcac
7141 tagtaggagg taatcaatgc tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
7201 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
7261 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
7321 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
7381 cagtacaatc tgctctgatg ccgcatgatc atctggcgga cctcttcgga ggtccgcttt
7441 tttttctcga gggtctcaac tagctcttca tgggtattct agagataatt tatttgaaaa
7501 ttcataagaa aagcaaacgt tacatgaatt gatgaaacaa tacaaagaca gataaagcca
7561 cgcacattta ggatattggc cgagattact gaatattgag taagatcacg gaatttctga
7621 caggagcatg tcttcaattc agcccaaatg gcagttgaaa tactcaaacc gccccatatg
7681 caggagcgga tcattcattg tttgtttggt tgcctttgcc aacatgggag tccaagagct
7741 ctcacttgta cagctcgtcc atgccgccgg tggagtggcg gccctcggcg cgttcgtact
7801 gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg ttgacgttgt
7861 aggcgccggg cagctgcacg ggcttcttgg ccttgtaggt ggtcttgacc tcagcgtcgt
7921 agtggccgcc gtccttcagc ttcagcctct gcttgatctc gcccttcagg gcgccgtcct
7981 cggggtacat ccgctcggag gaggcctccc agcccatggt cttcttctgc attacggggc
8041 cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta gatgaactcg ccgtcctgca
8101 gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc
8161 acttgaagcc ctcggggaag gacagcttca agtagtcggg gatgtcggcg gggtgcttca
8221 cgtaggcctt ggagccgtac atgaactgag gggacaggat gtcccaggcg aagggcaggg
8281 ggccaccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc
8341 cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc accttgaagc
8401 gcatgaactc cttgatgatg gccatgttat ccattgttcg taccgttttg ctatttgtgt
8461 atgttttctt gttttggttt gagtgaagag tgtagtgaag ggttggtttg tatttatagg
8521 tgagaaagga gatttgcatg gcaatcacgt gtaagaatgc atgcatgcat gagtgtgtgt
8581 ggagagagag agatgctctt acgttgttgt cgggattatg tgttggaaaa gagtgacaag
8641 tgacgaacaa aacaatacca ctcatcatag ctccgcttat gcatgctatg tgtgatttgt
8701 agctcttgtt ctgacatgac actatgaaca attttttaaa aagaaggtta aacttttatt
8761 taaataaaaa ccctatgtta atttggtttc aatcgagtaa gatgagaagt caatcttttg
8821 atggtagcta gtgatgttgg aaatatagtt gtgccagctt atctagacca agcatgcaca
8881 gctggcgttc tcagccagat ccccttgaca ggatatattg gcgggtaaac taagtcgctg
8941 tatgtgtttg tttgagatct catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
9001 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
9061 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
9121 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
9181 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
9241 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
9301 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
9361 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
9421 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
9481 gctctgctga agccagttac cttcggaaga agagttggta gctcttgatc cggcaaacaa
9541 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
9601 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
9661 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
9721 aattaaaaat gaagttttaa atcaatctaa agtatatatg tgtaacattg gtctagtgat
9781 tatttgccga ctaccttggt gatctcgcct ttcacgtagt gaacaaattc ttccaactga
9841 tctgcgcgcg aggccaagcg atcttcttgt ccaagataag cctgcctagc ttcaagtatg
9901 acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc
9961 gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc
10021 tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca
10081 aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca
10141 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
10201 tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
10261 gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg
10321 agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
10381 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc
10441 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
10501 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
10561 tccctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat
10621 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt
10681 tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca
10741 gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct
10801 ctcatcaacc gtggctccct cactttctgg ctggatgatg gggcgattca ggcgatcccc
10861 atccaacagc ccgccgtcga gcgggct
//
