| 载体名称: | pRU668 |
|---|---|
| 来源历史: | <- R. Schmitt |
| 质粒抗性: |
incT, Km抗性, Tc抗性, tra+, ts
|
| 载体说明: | 将天然存在的转座子Tn1721（11.4 kb）插入体内接合的Kmr ts质粒Rts1（Rts1：：Tn1721）；Tn1721本身属于Tn3家族，由一个重复的tet区和一个“小转座子”（Tn1722）组成，后者编码转座子功能；可用于大肠杆菌的诱变；大尺寸Rts1（217kb）的缺失阻止了任何Rts1：：Tn复制子的共转化。 |
| 宿主菌： | Escherichia coli K12 DH1 RU2945 |
| 培养条件： | LB培养基，30度 |
| 参考文献： |
Altenbuchner, J., Schmid, K., Schmitt, R. (1983). Tn1721-encoded tetracycline resistance: mapping of structural and functional genes mediating resistance. J.Bacteriol. 153 : 116-123 . Heffron, F., (1983). Tn3 and its relatives. In Mobile Genetic Elements , pp. 223-260. Edited by J. A. Shapiro, Academic Press. Schmitt, R., Altenbuchner, J., Wiebauer, K., Arnold, W., Pühler, A., Schöffl, F. (1981). Basis of transposition and gene amplification by Tn1721 and related tetracycline resistance transposons. Cold Spring Harb.Symp.Quant.Biol. 45 : 59-65 . Schmitt, R., Bernhard, E., Mattes, R. (1979). Characterization of Tn1721, a new transposon containing a tetracycline gene capable of amplification. Mol.Gen.Genet. 172 : 53-65 . Terawaki, Y., Kobayashi, Y., Matsumoto, H., Kamio, Y. (1981). Molecular cloning and mapping of a deletion derivative of the plasmid Rts1. Plasmid 6 : 222-234 . Ubben, D., Schmitt, R. (1986). Tn1721 derivatives for transposon mutagenesis, restriction mapping and nucleotide sequence analysis. Gene 41 : 145-152 . |
| 安全等级： | 1级 |
| 分离国家: | 原产国未知 |
| 存储时间: | 1990年3月16日前 |
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