| 载体名称: | pRU669 |
|---|---|
| 来源历史: | <- R. Schmitt |
| 质粒抗性: |
Cm抗性, incT, Km抗性, tra+, ts
|
| 载体说明: | 转座子Tn1725（8.9 kb）在体内插入接合Kmr ts质粒Rts1（Rts1：：Tn1725）；Tn1725本身是Tn1722的Cmr变体，通过将含有cat基因的R质粒Sa的3.3 kb HindIII片段连接到Tn1722的单个HindIII位点获得；可用于大肠杆菌中的诱变；Rts1的大尺寸（217kb）阻止任何Rts1：：Tn复制子的共转化。 |
| 宿主菌： | Escherichia coli K12 MM294 |
| 培养条件： | LB培养基，30度 |
| 参考文献： |
Altenbuchner, J., Schmid, K., Schmitt, R. (1983). Tn1721-encoded tetracycline resistance: mapping of structural and functional genes mediating resistance. J.Bacteriol. 153 : 116-123 . Datta, N., (1975). Epidemiology and classification of plasmids. In Microbiology 1974 , pp. 9-15. Edited by D. Schlessinger, Am. Soc. Microbiol. Heffron, F., (1983). Tn3 and its relatives. In Mobile Genetic Elements , pp. 223-260. Edited by J. A. Shapiro, Academic Press. Schmitt, R., Altenbuchner, J., Wiebauer, K., Arnold, W., Pühler, A., Schöffl, F. (1981). Basis of transposition and gene amplification by Tn1721 and related tetracycline resistance transposons. Cold Spring Harb.Symp.Quant.Biol. 45 : 59-65 . Terawaki, Y., Kobayashi, Y., Matsumoto, H., Kamio, Y. (1981). Molecular cloning and mapping of a deletion derivative of the plasmid Rts1. Plasmid 6 : 222-234 . Ubben, D., Schmitt, R. (1986). Tn1721 derivatives for transposon mutagenesis, restriction mapping and nucleotide sequence analysis. Gene 41 : 145-152 . Ward, J. M., Grinsted, J. (1982). Physical and genetic analysis of the Inc-W group plasmids R388, Sa, and R7K. Plasmid 7 : 239-250 . |
| 安全等级： | 1级 |
| 分离国家: | 原产国未知 |
| 存储时间: | 1990年3月16日前 |
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