质粒名称: | MSCV JMJD3 |
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出品公司: | 爱迪基因 |
目录编号: | 21212 |
存储实验室: | Paul Khavari |
载体骨架基本信息 |
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载体名称: | pMSCVpuro |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | 6300 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 哺乳动物表达,逆转录病毒 |
筛选标记: | 嘌呤霉素 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | JMJD3 |
物种来源: | H. sapiens (human) |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: |
Flag(插入N端子)
HA(插入件上的N端子)
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克隆方法: | 限制性内切酶 |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | pLXSN-5 |
3' 测序引物 | MSCV-rev |
文献引用方法 |
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材料和方法部分: | MSCV JMJD3 was a gift from Paul Khavari (Addgene plasmid # 21212 ; http://n2t.net/addgene:21212 ; RRID:Addgene_21212) |
参考文献部分: | Control of differentiation in a self-renewing mammalian tissue by the histone demethylase JMJD3. Sen GL, Webster DE, Barragan DI, Chang HY, Khavari PA. Genes Dev. 2008 Jul 15. 22(14):1865-70. 10.1101/gad.1673508 PubMed 18628393 |
买家导航
MSCVJMJD3载体基本信息
MSCVJMJD3载体图谱质粒图谱和多克隆位点信息
MSCVJMJD3载体简介
MSCVJMJD3载体序列
LOCUS MSCV JMJD3 11521 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS MSCV JMJD3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11521) AUTHORS Sen GL, Webster DE, Barragan DI, Chang HY, Khavari PA TITLE Control of differentiation in a self-renewing mammalian tissue by the histone demethylase JMJD3. JOURNAL Genes Dev. 2008 Jul 15. 22(14):1865-70. PUBMED 18628393 REFERENCE 2 (bases 1 to 11521) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11521 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(63..80) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(234..822) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(314..333) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(993..1853) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1616..1635 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1854..1958) /gene="bla" /label=AmpR promoter primer_bind 2026..2044 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(2082..2104) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 2204..2223 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 2417..2439 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 2567..2586 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" LTR 2786..3302 /label=5' LTR /note="5' long terminal repeat from murine embryonic stem cell virus" misc_feature 3366..3707 /label=MESV Psi /note="packaging signal of murine embryonic stem cell virus" CDS 3774..4190 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 4120..4142 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 4158..4174 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" regulatory 4203..4212 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 4212..4235 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 4248..4274 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" protein_bind 4290..4314 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS complement(4735..4746) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" CDS 5095..5118 /codon_start=1 /product="8xHis affinity tag" /label=8xHis /translation="HHHHHHHH" CDS 5098..5121 /codon_start=1 /product="8xHis affinity tag" /label=8xHis /translation="HHHHHHHH" CDS 6585..6614 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS 6588..6617 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" protein_bind complement(9408..9432) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 9462..10014 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(9606..9623) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 9833..9852 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 10026..10625 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(10026..10045) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 10522..10542 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 10701..11215 /label=3' LTR /note="3' long terminal repeat from murine embryonic stem cell virus" protein_bind 11377..11393 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(11401..11431) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11446..11467 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." ORIGIN 1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 961 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 1021 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 1081 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 1141 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 1201 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 1261 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 1321 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 1381 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 1441 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 1501 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 1561 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 1621 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 1681 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 1741 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 1801 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 1861 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 1921 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 1981 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 2041 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc 2101 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc 2161 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt 2221 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac 2281 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg 2341 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg 2401 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg aatggtgcat 2461 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga 2521 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga 2581 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt 2641 agaggcgatt agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga 2701 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt 2761 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag 2821 cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag 2881 atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc 2941 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag 3001 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt 3061 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc 3121 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc 3181 gtcgcccggg tacccgtatt cccaataaag cctcttgctg tttgcatccg aatcgtggac 3241 tcgctgatcc ttgggagggt ctcctcagat tgattgactg cccacctcgg gggtctttca 3301 tttggaggtt ccaccgagat ttggagaccc ctgcccaggg accaccgacc cccccgccgg 3361 gaggtaagct ggccagcggt cgtttcgtgt ctgtctctgt ctttgtgcgt gtttgtgccg 3421 gcatctaatg tttgcgcctg cgtctgtact agttagctaa ctagctctgt atctggcgga 3481 cccgtggtgg aactgacgag ttctgaacac ccggccgcaa ccctgggaga cgtcccaggg 3541 actttggggg ccgtttttgt ggcccgacct gaggaaggga gtcgatgtgg aatccgaccc 3601 cgtcaggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg 3661 aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg tctgctgcag cgctgcagca 3721 tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat tagggccaga 3781 ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc 3841 acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc 3901 caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg 3961 ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg 4021 tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta 4081 agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga 4141 ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattagat 4201 ctgccaccat ggactacaag gatgacgatg acaagctcga tggaggatac ccatacgatg 4261 ttccagatta cgctgctcga ggttatcaaa caagtttgta caaaaaagca ggctccgcgg 4321 ccgccccctt caccggatcc atgcatcggg cagtggatcc tccaggggcc cgcgctgcac 4381 gggaagcctt tgcccttggg ggcctgagct gtgctggggc ctggagctcc tgcccgcctc 4441 atccccctcc tcgtagcgca tggctgcctg gaggcagatg ctcagccagc attgggcagc 4501 ccccgcttcc tgctccccta cccccttcac atggcagtag ttctgggcac cccagcaaac 4561 catattatgc tccaggggcg cccactccaa gacccctcca tgggaagctg gaatccctgc 4621 atggctgtgt gcaggcattg ctccgggagc cagcccagcc agggctttgg gaacagcttg 4681 ggcaactgta cgagtcagag cacgatagtg aggaggccac acgctgctac cacagcgccc 4741 ttcgatacgg aggaagcttc gctgagctgg ggccccgcat tggccgactg cagcaggccc 4801 agctctggaa ctttcatact ggctcctgcc agcaccgagc caaggtcctg cccccactgg 4861 agcaagtgtg gaacttgcta caccttgagc acaaacggaa ctatggagcc aagcggggag 4921 gtcccccggt gaagcgagct gctgaacccc cagtggtgca gcctgtgcct cctgcagcac 4981 tctcaggccc ctcaggggag gagggcctca gccctggagg caagcgaagg agaggctgca 5041 actctgaaca gactggcctt cccccagggc tgccactgcc tccaccacca ttaccaccac 5101 caccaccacc accaccacca cccctgcctg gcctggctac cagcccccca tttcagctaa 5161 ccaagccagg gctgtggagt accctgcatg gagatgcctg gggcccagag cgcaagggtt 5221 cagcaccccc agagcgccag gagcagcggc actcgctgcc tcacccatat ccatacccag 5281 ctccagcgta caccgcgcac ccccctggcc accggctggt cccggctgct cccccaggcc 5341 caggcccccg ccccccagga gcagagagcc atggctgcct gcctgccacc cgtccccccg 5401 gaagtgacct tagagagagc agagttcaga ggtcgcggat ggactccagc gtttcaccag 5461 cagcaaccac cgcctgcgtg ccttacgccc cttcccggcc ccctggcctc cccggcacca 5521 ccaccagcag cagcagtagc agcagcagca acactggtct ccggggcgtg gagccgaacc 5581 caggcattcc cggcgctgac cattaccaaa ctcccgcgct ggaggtctct caccatggcc 5641 gcctggggcc ctcggcacac agcagtcgga aaccgttctt gggggctccc gctgccactc 5701 cccacctatc cctgccacct ggaccttcct cacccccccc cccccccctg tccccgcctc 5761 ttacgccccc caccaccccc tgcctggttg aagggtccgg cctgccgggc agcccgagag 5821 gatggagaga tcttagaaga gctcttcttt gggactgagg gacccccccg ccctgcccca 5881 ccacccctcc cccatcgcga gggcttcttg gggcctccgg cctcccgctt ttctgtgggc 5941 actcaggatt ctcacacccc tcccactccc ccaaccccaa ccaccagcag tagcaacagc 6001 aacagtggca gccacagcag cagccctgct gggcctgtgt cctttccccc accaccctat 6061 ctggccagaa gtatagaccc ccttccccgg cctcccagcc cagcacagaa cccccaggac 6121 ccacctcttg tacccctgac tcttgccctg cctccagccc ctccttcctc ctgccaccaa 6181 aatacctcag gaagcttcag gcgcccggag agcccccggc ccagggtctc cttcccaaag 6241 acccccgagg tggggccggg gccaccccca ggccccctga gtaaagcccc ccagcctgtg 6301 ccgcccgggg ttggggagct gcctgcccga ggccctcgac tctttgattt tccccccact 6361 ccgctggagg accagtttga ggagccagcc gaattcaaga tcctacctga tgggctggcc 6421 aacatcatga agatgctgga cgaatccatt cgcaaggaag aggaacagca acaacacgaa 6481 gcaggcgtgg ccccccaacc cccgctgaag gagccctttg catctctgca gtctcctttc 6541 cccaccgaca cagcccccac cactactgct cctgctgtcg ccgtcaccac caccaccacc 6601 accaccacca ccaccacggc cacccaggaa gaggagaaga agccaccacc agccctacca 6661 ccaccaccgc ctctagccaa gttccctcca ccctctcagc cacagccacc accaccccca 6721 ccccccagcc cggccagcct gctcaaatcc ttggcctccg tgctggaggg acaaaagtac 6781 tgttatcggg ggactggagc agctgtttcc acccggcctg ggcccttgcc caccactcag 6841 tattcccctg gccccccatc aggtgctacc gccctgccgc ccacctcagc ggcccctagc 6901 gcccagggct ccccacagcc ctctgcttcc tcgtcatctc agttctctac ctcaggcggg 6961 ccctgggccc gggagcgcag ggcgggcgaa gagccagtcc cgggccccat gacccccacc 7021 caaccgcccc cacccctatc tctgccccct gctcgctctg agtctgaggt gctagaagag 7081 atcagccggg cttgcgagac ccttgtggag cgggtgggcc ggagtgccac tgacccagcc 7141 gacccagtgg acacagcaga gccagcggac agtgggactg agcgactgct gccccccgca 7201 caggccaagg aggaggctgg cggggtggcg gcagtgtcag gcagctgtaa gcggcgacag 7261 aaggagcatc agaaggagca tcggcggcac aggcgggcct gtaaggacag tgtgggtcgt 7321 cggccccgtg agggcagggc aaaggccaag gccaaggtcc ccaaagaaaa gagccgccgg 7381 gtgctgggga acctggacct gcagagcgag gagatccagg gtcgtgagaa gtcccggccc 7441 gatcttggcg gggcctccaa ggccaagcca cccacagctc cagcccctcc atcagctcct 7501 gcaccttctg cccagcccac acccccgtca gcctctgtcc ctggaaagaa ggctcgggag 7561 gaagccccag ggccaccggg tgtcagccgg gccgacatgc tgaagctgcg ctcacttagt 7621 gaggggcccc ccaaggagct gaagatccgg ctcatcaagg tagagagtgg tgacaaggag 7681 acctttatcg cctctgaggt ggaagagcgg cggctgcgca tggcagacct caccatcagc 7741 cactgtgctg ctgacgtcgt gcgcgccagc aggaatgcca aggtgaaagg gaagtttcga 7801 gagtcctacc tttcccctgc ccagtctgtg aaaccgaaga tcaacactga ggagaagctg 7861 ccccgggaaa aactcaaccc ccctacaccc agcatctatc tggagagcaa acgggatgcc 7921 ttctcacctg tcctgctgca gttctgtaca gaccctcgaa atcccatcac agtgatccgg 7981 ggcctggcgg gctccctgcg gctcaacttg ggcctcttct ccaccaagac cctggtggaa 8041 gcgagtggcg aacacaccgt ggaagttcgc acccaggtgc agcagccctc agatgagaac 8101 tgggatctga caggcactcg gcagatctgg ccttgtgaga gctcccgttc ccacaccacc 8161 attgccaagt acgcacagta ccaggcctca tccttccagg agtctctgca ggaggagaag 8221 gagagtgagg atgaggagtc agaggagcca gacagcacca ctggaacccc tcctagcagc 8281 gcaccagacc cgaagaacca tcacatcatc aagtttggca ccaacatcga cttgtctgat 8341 gctaagcggt ggaagcccca gctgcaggag ctgctaaagc tgcccgcctt catgcgggta 8401 acatccacgg gcaacatgct gagccacgtg ggccacacca tcctgggcat gaacacggtg 8461 cagctgtaca tgaaggtgcc cggcagccga acgccaggcc accaggagaa taacaacttc 8521 tgctccgtca acatcaacat tggcccaggc gactgcgagt ggttcgcggt gcacgagcac 8581 tactgggaga ccatcagcgc tttctgtgat cggcacggcg tggactactt gacgggttcc 8641 tggtggccaa tcctggatga tctctatgca tccaatattc ctgtgtaccg cttcgtgcag 8701 cgacccggag acctcgtgtg gattaatgcg gggactgtgc actgggtgca ggccaccggc 8761 tggtgcaaca acattgcctg gaacgtgggg cccctcaccg cctatcagta ccagctggcc 8821 ctggaacgat acgagtggaa tgaggtgaag aacgtcaaat ccatcgtgcc catgattcac 8881 gtgtcatgga acgtggctcg cacggtcaaa atcagcgacc ccgacttgtt caagatgatc 8941 aagttctgcc tgctgcagtc catgaagcac tgccaggtgc aacgcgagag cctggtgcgg 9001 gcagggaaga aaatcgctta ccagggccgt gtcaaggacg agccagccta ctactgcaac 9061 gagtgcgatg tggaggtgtt taacatcctg ttcgtgacaa gtgagaatgg cagccgcaac 9121 acgtacctgg tacactgcga gggctgtgcc cggcgccgca gcgcaggcct gcagggcgtg 9181 gtggtgctgg agcagtaccg cactgaggag ctggctcagg cctacgacgc cttcacgctg 9241 gtgagggccc ggcgggcgcg cgggcagcgg aggagggcac tggggcaggc tgcagggacg 9301 ggcttcggga gcccggccgc gcctttccct gagcccccgc cggctttctc cccccaggcc 9361 ccagccagca cgtcgcgatg actcgaggcg aaagggtggg cgcgccgacc cagctttctt 9421 gtacaaagtg gttcgataac gaattccgcc ccccccccct aacgttactg gccgaagccg 9481 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt 9541 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct 9601 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct 9661 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc 9721 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc 9781 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc 9841 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc 9901 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct 9961 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca 10021 caaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg 10081 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc 10141 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 10201 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 10261 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 10321 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 10381 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 10441 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 10501 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 10561 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg 10621 cctgatgcat cgatgtcgac ctgcagccaa gcttatcgat aaaataaaag attttattta 10681 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag 10741 taacgccatt ttgcaaggca tggaaaatac ataactgaga atagagaagt tcagatcaag 10801 gttaggaaca gagagacagc agaatatggg ccaaacagga tatctgtggt aagcagttcc 10861 tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc tcagcagttt 10921 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta 10981 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct 11041 caataaaaga gcccacaacc cctcactcgg cgcgccagtc ctccgataga ctgcgtcgcc 11101 cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt 11161 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatgggta 11221 acagtttctt gaagttggag aacaacattc tgagggtagg agtcgaatat taagtaatcc 11281 tgactcaatt agccactgtt ttgaatccac atactccaat actcctgaaa tagttcatta 11341 tggacagcgc agaaagagct ggggagaatt gtgaaattgt tatccgctca caattccaca 11401 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 11461 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 11521 g //
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