出品公司: | NIH | |
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载体名称: | pNL4-3、pNL4.3 | |
载体又名: | HIV-1 NL4-3 Infectious Molecular Clone (pNL4-3)、 HIV-1 vector pNL4-3、pNL4-3载体、pNL4.3载体 | |
质粒类型: | 哺乳动物细胞载体;逆病毒包装载体 | |
高拷贝/低拷贝: | 高拷贝 | |
克隆方法: | 限制性内切酶,多克隆位点 | |
启动子: | CMV | |
载体大小: | 14825 bp | |
5' 测序引物及序列: | -- | |
3' 测序引物及序列: | -- | |
载体序列: | Genbank: AF324493 | |
存储人: | Dr. Malcolm Mar | |
载体抗性: | 氨苄青霉素 | |
筛选标记: | 嘌呤霉素(Puromycin) | |
克隆菌株: | DH5α 等 | |
宿主细胞(系): | 常规细胞系(293、CV-1、CHO等) | |
备注: |
一个包含有全长基因,复制能力强,能够感染HIV-1亚型B NL4-3蛋白的分子克隆载体。
这个载体产生的病毒利用CXCR4作为共同受体。
该载体为大小为14825 bp,包括插入片段。
这个嵌合载体的来源是HIV-1ny5分离物5’ 从基因组DNA中直接克隆的LAV分离物3’。前病毒NY5(5’SmaI和 3’EcoRI)前病毒3’片段(5’EcoRI 和3’NruI)被钝端克隆到PvuII位点的pUC18中 删除多段连接片段后。
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产品目录号: | #114 | |
稳定性: | 稳表达 | |
组成型/诱导型: | 组成型 | |
病毒/非病毒: | 非病毒 | |
生物危害性: | 无 | |
参考文献: |
Adachi, A., Gendelman, H. E., Koenig, S., Folks, T., Willey, R., Rabson, A., & Martin, M. A. (1986). Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone. J Virol, 59(2), 284-291. PUBMED
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载体说明书: | pNL4-3载体说明书 | |
相关载体: |
pNL4-3-Luc-R-E- NL4-3-ΔEnv-EGFP |
买家导航
pNL4-3载体 pNL4.3载体HIV-1质粒基本信息
pNL4-3质粒图谱 pNL4.3载体HIV-1载体图谱和pNL4-3载体序列 pNL4.3载体HIV-1质粒序列多克隆位点信息
pNL4-3质粒 pNL4.3载体HIV-1载体简介
pNL4-3质粒序列 pNL4.3载体HIV-1载体序列
LOCUS pNL4-3 14825 bp DNA circular SYN 25-JUL-2016 DEFINITION HIV-1 vector pNL4-3, complete sequence. ACCESSION AF324493 VERSION AF324493.2 KEYWORDS . SOURCE HIV-1 vector pNL4-3 ORGANISM HIV-1 vector pNL4-3 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 9709) AUTHORS Adachi,A., Gendelman,H.E., Koenig,S., Folks,T., Willey,R., Rabson,A. and Martin,M.A. TITLE Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone JOURNAL J. Virol. 59 (2), 284-291 (1986) PUBMED 3016298 REFERENCE 2 (bases 1 to 14825) AUTHORS Bosche,W.J., Poon,D.T.K., Ott,D.E., Hu,W.-S. and Gorelick,R.J. TITLE Complete Plasmid Sequence of pNL4-3 JOURNAL Unpublished REFERENCE 3 (bases 1 to 14825) AUTHORS Bosche,W.J., Poon,D.T.K., Ott,D.E., Hu,W.-S. and Gorelick,R.J. TITLE Direct Submission JOURNAL Submitted (28-NOV-2000) AIDS Vaccine Program, SAIC Frederick and Drug Resistance Program, National Cancer Institute at Frederick, PO Box B, Frederick, Maryland 21702-1202, USA REFERENCE 4 (bases 1 to 14825) AUTHORS Strebel,K.J. and Martin,M.A. TITLE Direct Submission JOURNAL Submitted (24-MAY-2010) NIAID, Viral Biochemistry, National Institutes of Health, Bethesda, MD, USA REMARK Sequence update by submitter COMMENT On May 24, 2010 this sequence version replaced AF324493.1. FEATURES Location/Qualifiers source 1..14825 /organism="HIV-1 vector pNL4-3" /mol_type="other DNA" /db_xref="taxon:151458" /plasmid="pNL4-3" /focus source 1..9709 /organism="Human immunodeficiency virus 1" /proviral /mol_type="other DNA" /strain="NY5/BRU (LAV-1)" /db_xref="taxon:11676" /note="from sequence deposited in GenBank Accession Number M19921" repeat_region 1..634 /note="5' long terminal repeat" /rpt_type=long_terminal_repeat repeat_region 454..550 /note="R; 5' copy" CDS 790..2292 /codon_start=1 /transl_table=11 /product="gag polyprotein" /protein_id="AAK08483.1" /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERF AVNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALD KIEEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVE EKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPV HAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRM YSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTIL KALGPGATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKC FNCGKEGHIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQ SRPEPTAPPEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ" CDS <2085..5096 /codon_start=1 /transl_table=11 /product="pol polyprotein" /protein_id="AAK08484.2" /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEA GADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKM IGGIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPI ETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAI KKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLD KDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIV IYQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPD KWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPL TEEAELELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGK YARMKGAHTNDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWI PEWEFVNTPPLVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPL TDTTNQKTELQAIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIK KEKVYLAWVPAHKGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASD FNLPPVVAKEIVASCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGY IEAEVIPAETGQETAYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGI PYNPQSQGVIESMNKELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGER IVDIIATDIQTKELQKQITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDI KVVPRRKAKIIRDYGKQMAGDDCVASRQDED" misc_difference 2421 /note="compared to sequence deposited in GenBank Accession Number M19921; results in arginine to glycine substitution" /replace="g" CDS 5041..5619 /codon_start=1 /transl_table=11 /product="vif protein" /protein_id="AAK08482.1" /translation="MENRWQVMIVWQVDRMRINTWKRLVKHHMYISRKAKDWFYRHHY ESTNPKISSEVHIPLGDAKLVITTYWGLHTGERDWHLGQGVSIEWRKKRYSTQVDPDL ADQLIHLHYFDCFSESAIRNTILGRIVSPRCEYQAGHNKVGSLQYLALAALIKPKQIK PPLPSVRKLTEDRWNKPQKTKGHRGSHTMNGH" CDS 5559..5849 /codon_start=1 /transl_table=11 /product="vpr protein" /protein_id="AAK08485.1" /translation="MEQAPEDQGPQREPYNEWTLELLEELKSEAVRHFPRIWLHNLGQ HIYETYGDTWAGVEAIIRILQQLLFIHFRIGCRHSRIGVTRQRRARNGASRS" misc_feature 5743..5748 /note="EcoRI site of recombination" CDS join(5830..6044,8369..8414) /codon_start=1 /transl_table=11 /product="tat protein" /protein_id="AAK08486.1" /translation="MEPVDPRLEPWKHPGSQPKTACTNCYCKKCCFHCQVCFMTKALG ISYGRKKRRQRRRAHQNSQTHQASLSKQPTSQSRGDPTGPKE" CDS join(5969..6044,8369..8643) /codon_start=1 /transl_table=11 /product="rev protein" /protein_id="AAK08487.1" /translation="MAGRSGDSDEELIRTVRLIKLLYQSNPPPNPEGTRQARRNRRRR WRERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQI LVESPTVLESGTKE" CDS 6061..6306 /codon_start=1 /transl_table=11 /product="vpu protein" /protein_id="AAK08488.1" /translation="MQPIIVAIVALVVAIIIAIVVWSIVIIEYRKILRQRKIDRLIDR LIERAEDSGNESEGEVSALVEMGVEMGHHAPWDIDDL" CDS 6221..8785 /codon_start=1 /transl_table=11 /product="envelope polyprotein" /protein_id="AAK08489.2" /translation="MRVKEKYQHLWRWGWKWGTMLLGILMICSATEKLWVTVYYGVPV WKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLVNVTENFNMWKNDMVE QMHEDIISLWDQSLKPCVKLTPLCVSLKCTDLKNDTNTNSSSGRMIMEKGEIKNCSFN ISTSIRDKVQKEYAFFYKLDIVPIDNTSYRLISCNTSVITQACPKVSFEPIPIHYCAP AGFAILKCNNKTFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEDVVIRSANFT DNAKTIIVQLNTSVEINCTRPNNNTRKSIRIQRGPGRAFVTIGKIGNMRQAHCNISRA KWNATLKQIASKLREQFGNNKTIIFKQSSGGDPEIVTHSFNCGGEFFYCNSTQLFNST WFNSTWSTEGSNNTEGSDTITLPCRIKQFINMWQEVGKAMYAPPISGQIRCSSNITGL LLTRDGGNNNNGSEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREK RAVGIGALFLGFLGAAGSTMGAASMTLTVQARQLLSDIVQQQNNLLRAIEAQQHLLQL TVWGIKQLQARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNNM TWMEWDREINNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKL FIMIVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQTHLPIPRGPDRPEGIEEEGGERDR DRSIRLVNGSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLL QYWSQELKNSAVNLLNATAIAVAEGTDRVIEVLQAAYRAIRHIPRRIRQGLERILL" CDS 8787..9407 /codon_start=1 /transl_table=11 /product="nef protein" /protein_id="AAK08490.1" /translation="MGGKWSKSSVIGWPAVRERMRRAEPAADGVGAVSRDLEKHGAIT SSNTAANNAACAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH SQRRQDILDLWIYHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGE NTSLLHPVSLHGMDDPEREVLEWRFDSRLAFHHVARELHPEYFKNC" repeat_region 9076..9709 /note="3' long terminal repeat" /rpt_type=long_terminal_repeat repeat_region 9529..9626 /note="R; 3' copy" regulatory 9602..9607 /regulatory_class="polyA_signal_sequence" misc_feature 9710..11283 /note="3' cellular genomic sequence" CDS complement(12279..13139) /note="from pUC vector" /codon_start=1 /transl_table=11 /product="beta-lactamase" /protein_id="AAK08491.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" misc_feature 13648..14825 /note="5' cellular genomic sequence" ORIGIN 1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac 121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca 181 atgaaggaga gaacaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg 241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag 301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg 361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa 841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca 901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt 961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca 1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc 1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa 1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac 1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatgc atgggtaaaa 1261 gtagtagaag agaaggcttt cagcccagaa gtaataccca tgttttcagc attatcagaa 1321 ggagccaccc cacaagattt aaataccatg ctaaacacag tggggggaca tcaagcagcc 1381 atgcaaatgt taaaagagac catcaatgag gaagctgcag aatgggatag attgcatcca 1441 gtgcatgcag ggcctattgc accaggccag atgagagaac caaggggaag tgacatagca 1501 ggaactacta gtacccttca ggaacaaata ggatggatga cacataatcc acctatccca 1561 gtaggagaaa tctataaaag atggataatc ctgggattaa ataaaatagt aagaatgtat 1621 agccctacca gcattctgga cataagacaa ggaccaaagg aaccctttag agactatgta 1681 gaccgattct ataaaactct aagagccgag caagcttcac aagaggtaaa aaattggatg 1741 acagaaacct tgttggtcca aaatgcgaac ccagattgta agactatttt aaaagcattg 1801 ggaccaggag cgacactaga agaaatgatg acagcatgtc agggagtggg gggacccggc 1861 cataaagcaa gagttttggc tgaagcaatg agccaagtaa caaatccagc taccataatg 1921 atacagaaag gcaattttag gaaccaaaga aagactgtta agtgtttcaa ttgtggcaaa 1981 gaagggcaca tagccaaaaa ttgcagggcc cctaggaaaa agggctgttg gaaatgtgga 2041 aaggaaggac accaaatgaa agattgtact gagagacagg ctaatttttt agggaagatc 2101 tggccttccc acaagggaag gccagggaat tttcttcaga gcagaccaga gccaacagcc 2161 ccaccagaag agagcttcag gtttggggaa gagacaacaa ctccctctca gaagcaggag 2221 ccgatagaca aggaactgta tcctttagct tccctcagat cactctttgg cagcgacccc 2281 tcgtcacaat aaagataggg gggcaattaa aggaagctct attagataca ggagcagatg 2341 atacagtatt agaagaaatg aatttgccag gaagatggaa accaaaaatg atagggggaa 2401 ttggaggttt tatcaaagta agacagtatg atcagatact catagaaatc tgcggacata 2461 aagctatagg tacagtatta gtaggaccta cacctgtcaa cataattgga agaaatctgt 2521 tgactcagat tggctgcact ttaaattttc ccattagtcc tattgagact gtaccagtaa 2581 aattaaagcc aggaatggat ggcccaaaag ttaaacaatg gccattgaca gaagaaaaaa 2641 taaaagcatt agtagaaatt tgtacagaaa tggaaaagga aggaaaaatt tcaaaaattg 2701 ggcctgaaaa tccatacaat actccagtat ttgccataaa gaaaaaagac agtactaaat 2761 ggagaaaatt agtagatttc agagaactta ataagagaac tcaagatttc tgggaagttc 2821 aattaggaat accacatcct gcagggttaa aacagaaaaa atcagtaaca gtactggatg 2881 tgggcgatgc atatttttca gttcccttag ataaagactt caggaagtat actgcattta 2941 ccatacctag tataaacaat gagacaccag ggattagata tcagtacaat gtgcttccac 3001 agggatggaa aggatcacca gcaatattcc agtgtagcat gacaaaaatc ttagagcctt 3061 ttagaaaaca aaatccagac atagtcatct atcaatacat ggatgatttg tatgtaggat 3121 ctgacttaga aatagggcag catagaacaa aaatagagga actgagacaa catctgttga 3181 ggtggggatt taccacacca gacaaaaaac atcagaaaga acctccattc ctttggatgg 3241 gttatgaact ccatcctgat aaatggacag tacagcctat agtgctgcca gaaaaggaca 3301 gctggactgt caatgacata cagaaattag tgggaaaatt gaattgggca agtcagattt 3361 atgcagggat taaagtaagg caattatgta aacttcttag gggaaccaaa gcactaacag 3421 aagtagtacc actaacagaa gaagcagagc tagaactggc agaaaacagg gagattctaa 3481 aagaaccggt acatggagtg tattatgacc catcaaaaga cttaatagca gaaatacaga 3541 agcaggggca aggccaatgg acatatcaaa tttatcaaga gccatttaaa aatctgaaaa 3601 caggaaagta tgcaagaatg aagggtgccc acactaatga tgtgaaacaa ttaacagagg 3661 cagtacaaaa aatagccaca gaaagcatag taatatgggg aaagactcct aaatttaaat 3721 tacccataca aaaggaaaca tgggaagcat ggtggacaga gtattggcaa gccacctgga 3781 ttcctgagtg ggagtttgtc aatacccctc ccttagtgaa gttatggtac cagttagaga 3841 aagaacccat aataggagca gaaactttct atgtagatgg ggcagccaat agggaaacta 3901 aattaggaaa agcaggatat gtaactgaca gaggaagaca aaaagttgtc cccctaacgg 3961 acacaacaaa tcagaagact gagttacaag caattcatct agctttgcag gattcgggat 4021 tagaagtaaa catagtgaca gactcacaat atgcattggg aatcattcaa gcacaaccag 4081 ataagagtga atcagagtta gtcagtcaaa taatagagca gttaataaaa aaggaaaaag 4141 tctacctggc atgggtacca gcacacaaag gaattggagg aaatgaacaa gtagataaat 4201 tggtcagtgc tggaatcagg aaagtactat ttttagatgg aatagataag gcccaagaag 4261 aacatgagaa atatcacagt aattggagag caatggctag tgattttaac ctaccacctg 4321 tagtagcaaa agaaatagta gccagctgtg ataaatgtca gctaaaaggg gaagccatgc 4381 atggacaagt agactgtagc ccaggaatat ggcagctaga ttgtacacat ttagaaggaa 4441 aagttatctt ggtagcagtt catgtagcca gtggatatat agaagcagaa gtaattccag 4501 cagagacagg gcaagaaaca gcatacttcc tcttaaaatt agcaggaaga tggccagtaa 4561 aaacagtaca tacagacaat ggcagcaatt tcaccagtac tacagttaag gccgcctgtt 4621 ggtgggcggg gatcaagcag gaatttggca ttccctacaa tccccaaagt caaggagtaa 4681 tagaatctat gaataaagaa ttaaagaaaa ttataggaca ggtaagagat caggctgaac 4741 atcttaagac agcagtacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga 4801 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta 4861 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca 4921 gagatccagt ttggaaagga ccagcaaagc tcctctggaa aggtgaaggg gcagtagtaa 4981 tacaagataa tagtgacata aaagtagtgc caagaagaaa agcaaagatc atcagggatt 5041 atggaaaaca gatggcaggt gatgattgtg tggcaagtag acaggatgag gattaacaca 5101 tggaaaagat tagtaaaaca ccatatgtat atttcaagga aagctaagga ctggttttat 5161 agacatcact atgaaagtac taatccaaaa ataagttcag aagtacacat cccactaggg 5221 gatgctaaat tagtaataac aacatattgg ggtctgcata caggagaaag agactggcat 5281 ttgggtcagg gagtctccat agaatggagg aaaaagagat atagcacaca agtagaccct 5341 gacctagcag accaactaat tcatctgcac tattttgatt gtttttcaga atctgctata 5401 agaaatacca tattaggacg tatagttagt cctaggtgtg aatatcaagc aggacataac 5461 aaggtaggat ctctacagta cttggcacta gcagcattaa taaaaccaaa acagataaag 5521 ccacctttgc ctagtgttag gaaactgaca gaggacagat ggaacaagcc ccagaagacc 5581 aagggccaca gagggagcca tacaatgaat ggacactaga gcttttagag gaacttaaga 5641 gtgaagctgt tagacatttt cctaggatat ggctccataa cttaggacaa catatctatg 5701 aaacttacgg ggatacttgg gcaggagtgg aagccataat aagaattctg caacaactgc 5761 tgtttatcca tttcagaatt gggtgtcgac atagcagaat aggcgttact cgacagagga 5821 gagcaagaaa tggagccagt agatcctaga ctagagccct ggaagcatcc aggaagtcag 5881 cctaaaactg cttgtaccaa ttgctattgt aaaaagtgtt gctttcattg ccaagtttgt 5941 ttcatgacaa aagccttagg catctcctat ggcaggaaga agcggagaca gcgacgaaga 6001 gctcatcaga acagtcagac tcatcaagct tctctatcaa agcagtaagt agtacatgta 6061 atgcaaccta taatagtagc aatagtagca ttagtagtag caataataat agcaatagtt 6121 gtgtggtcca tagtaatcat agaatatagg aaaatattaa gacaaagaaa aatagacagg 6181 ttaattgata gactaataga aagagcagaa gacagtggca atgagagtga aggagaagta 6241 tcagcacttg tggagatggg ggtggaaatg gggcaccatg ctccttggga tattgatgat 6301 ctgtagtgct acagaaaaat tgtgggtcac agtctattat ggggtacctg tgtggaagga 6361 agcaaccacc actctatttt gtgcatcaga tgctaaagca tatgatacag aggtacataa 6421 tgtttgggcc acacatgcct gtgtacccac agaccccaac ccacaagaag tagtattggt 6481 aaatgtgaca gaaaatttta acatgtggaa aaatgacatg gtagaacaga tgcatgagga 6541 tataatcagt ttatgggatc aaagcctaaa gccatgtgta aaattaaccc cactctgtgt 6601 tagtttaaag tgcactgatt tgaagaatga tactaatacc aatagtagta gcgggagaat 6661 gataatggag aaaggagaga taaaaaactg ctctttcaat atcagcacaa gcataagaga 6721 taaggtgcag aaagaatatg cattctttta taaacttgat atagtaccaa tagataatac 6781 cagctatagg ttgataagtt gtaacacctc agtcattaca caggcctgtc caaaggtatc 6841 ctttgagcca attcccatac attattgtgc cccggctggt tttgcgattc taaaatgtaa 6901 taataagacg ttcaatggaa caggaccatg tacaaatgtc agcacagtac aatgtacaca 6961 tggaatcagg ccagtagtat caactcaact gctgttaaat ggcagtctag cagaagaaga 7021 tgtagtaatt agatctgcca atttcacaga caatgctaaa accataatag tacagctgaa 7081 cacatctgta gaaattaatt gtacaagacc caacaacaat acaagaaaaa gtatccgtat 7141 ccagagggga ccagggagag catttgttac aataggaaaa ataggaaata tgagacaagc 7201 acattgtaac attagtagag caaaatggaa tgccacttta aaacagatag ctagcaaatt 7261 aagagaacaa tttggaaata ataaaacaat aatctttaag caatcctcag gaggggaccc 7321 agaaattgta acgcacagtt ttaattgtgg aggggaattt ttctactgta attcaacaca 7381 actgtttaat agtacttggt ttaatagtac ttggagtact gaagggtcaa ataacactga 7441 aggaagtgac acaatcacac tcccatgcag aataaaacaa tttataaaca tgtggcagga 7501 agtaggaaaa gcaatgtatg cccctcccat cagtggacaa attagatgtt catcaaatat 7561 tactgggctg ctattaacaa gagatggtgg taataacaac aatgggtccg agatcttcag 7621 acctggagga ggcgatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt 7681 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga 7741 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac 7801 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctgatatagt 7861 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac 7921 agtctggggc atcaaacagc tccaggcaag aatcctggct gtggaaagat acctaaagga 7981 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc 8041 ttggaatgct agttggagta ataaatctct ggaacagatt tggaataaca tgacctggat 8101 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc 8161 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt 8221 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt 8281 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag 8341 gcagggatat tcaccattat cgtttcagac ccacctccca atcccgaggg gacccgacag 8401 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt 8461 gaacggatcc ttagcactta tctgggacga tctgcggagc ctgtgcctct tcagctacca 8521 ccgcttgaga gacttactct tgattgtaac gaggattgtg gaacttctgg gacgcagggg 8581 gtgggaagcc ctcaaatatt ggtggaatct cctacagtat tggagtcagg aactaaagaa 8641 tagtgctgtt aacttgctca atgccacagc catagcagta gctgagggga cagatagggt 8701 tatagaagta ttacaagcag cttatagagc tattcgccac atacctagaa gaataagaca 8761 gggcttggaa aggattttgc tataagatgg gtggcaagtg gtcaaaaagt agtgtgattg 8821 gatggcctgc tgtaagggaa agaatgagac gagctgagcc agcagcagat ggggtgggag 8881 cagtatctcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctaaca 8941 atgctgcttg tgcctggcta gaagcacaag aggaggaaga ggtgggtttt ccagtcacac 9001 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 9061 aagaaaaggg gggactggaa gggctaattc actcccaaag aagacaagat atccttgatc 9121 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag 9181 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata 9241 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg 9301 gaatggatga ccctgagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc 9361 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgacat cgagcttgct 9421 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt 9481 ggcgagccct cagatgctgc atataagcag ctgctttttg cctgtactgg gtctctctgg 9541 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 9601 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 9661 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcac ccaggaggta 9721 gaggttgcag tgagccaaga tcgcgccact gcattccagc ctgggcaaga aaacaagact 9781 gtctaaaata ataataataa gttaagggta ttaaatatat ttatacatgg aggtcataaa 9841 aatatatata tttgggctgg gcgcagtggc tcacacctgc gcccggccct ttgggaggcc 9901 gaggcaggtg gatcacctga gtttgggagt tccagaccag cctgaccaac atggagaaac 9961 cccttctctg tgtattttta gtagatttta ttttatgtgt attttattca caggtatttc 10021 tggaaaactg aaactgtttt tcctctactc tgataccaca agaatcatca gcacagagga 10081 agacttctgt gatcaaatgt ggtgggagag ggaggttttc accagcacat gagcagtcag 10141 ttctgccgca gactcggcgg gtgtccttcg gttcagttcc aacaccgcct gcctggagag 10201 aggtcagacc acagggtgag ggctcagtcc ccaagacata aacacccaag acataaacac 10261 ccaacaggtc caccccgcct gctgcccagg cagagccgat tcaccaagac gggaattagg 10321 atagagaaag agtaagtcac acagagccgg ctgtgcggga gaacggagtt ctattatgac 10381 tcaaatcagt ctccccaagc attcggggat cagagttttt aaggataact tagtgtgtag 10441 ggggccagtg agttggagat gaaagcgtag ggagtcgaag gtgtcctttt gcgccgagtc 10501 agttcctggg tgggggccac aagatcggat gagccagttt atcaatccgg gggtgccagc 10561 tgatccatgg agtgcagggt ctgcaaaata tctcaagcac tgattgatct taggttttac 10621 aatagtgatg ttaccccagg aacaatttgg ggaaggtcag aatcttgtag cctgtagctg 10681 catgactcct aaaccataat ttcttttttg tttttttttt tttatttttg agacagggtc 10741 tcactctgtc acctaggctg gagtgcagtg gtgcaatcac agctcactgc agcctcaacg 10801 tcgtaagctc aagcgatcct cccacctcag cctgcctggt agctgagact acaagcgacg 10861 ccccagttaa tttttgtatt tttggtagag gcagcgtttt gccgtgtggc cctggctggt 10921 ctcgaactcc tgggctcaag tgatccagcc tcagcctccc aaagtgctgg gacaaccggg 10981 gccagtcact gcacctggcc ctaaaccata atttctaatc ttttggctaa tttgttagtc 11041 ctacaaaggc agtctagtcc ccaggcaaaa agggggtttg tttcgggaaa gggctgttac 11101 tgtctttgtt tcaaactata aactaagttc ctcctaaact tagttcggcc tacacccagg 11161 aatgaacaag gagagcttgg aggttagaag cacgatggaa ttggttaggt cagatctctt 11221 tcactgtctg agttataatt ttgcaatggt ggttcaaaga ctgcccgctt ctgacaccag 11281 tcgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 11341 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 11401 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 11461 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 11521 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 11581 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 11641 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 11701 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 11761 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 11821 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 11881 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 11941 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 12001 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 12061 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 12121 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 12181 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 12241 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 12301 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 12361 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 12421 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 12481 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 12541 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 12601 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 12661 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 12721 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 12781 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 12841 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 12901 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 12961 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 13021 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 13081 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 13141 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 13201 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 13261 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 13321 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 13381 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 13441 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag 13501 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga 13561 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg 13621 gtgcgggcct cttcgctatt acgccagggg aggcagagat tgcagtaagc tgagatcgca 13681 gcactgcact ccagcctggg cgacagagta agactctgtc tcaaaaataa aataaataaa 13741 tcaatcagat attccaatct tttcctttat ttatttattt attttctatt ttggaaacac 13801 agtccttcct tattccagaa ttacacatat attctatttt tctttatatg ctccagtttt 13861 ttttagacct tcacctgaaa tgtgtgtata caaaatctag gccagtccag cagagcctaa 13921 aggtaaaaaa taaaataata aaaaataaat aaaatctagc tcactccttc acatcaaaat 13981 ggagatacag ctgttagcat taaataccaa ataacccatc ttgtcctcaa taattttaag 14041 cgcctctctc caccacatct aactcctgtc aaaggcatgt gccccttccg ggcgctctgc 14101 tgtgctgcca accaactggc atgtggactc tgcagggtcc ctaactgcca agccccacag 14161 tgtgccctga ggctgcccct tccttctagc ggctgccccc actcggcttt gctttcccta 14221 gtttcagtta cttgcgttca gccaaggtct gaaactaggt gcgcacagag cggtaagact 14281 gcgagagaaa gagaccagct ttacaggggg tttatcacag tgcaccctga cagtcgtcag 14341 cctcacaggg ggtttatcac attgcaccct gacagtcgtc agcctcacag ggggtttatc 14401 acagtgcacc cttacaatca ttccatttga ttcacaattt ttttagtctc tactgtgcct 14461 aacttgtaag ttaaatttga tcagaggtgt gttcccagag gggaaaacag tatatacagg 14521 gttcagtact atcgcatttc aggcctccac ctgggtcttg gaatgtgtcc cccgaggggt 14581 gatgactacc tcagttggat ctccacaggt cacagtgaca caagataacc aagacacctc 14641 ccaaggctac cacaatgggc cgccctccac gtgcacatgg ccggaggaac tgccatgtcg 14701 gaggtgcaag cacacctgcg catcagagtc cttggtgtgg agggagggac cagcgcagct 14761 tccagccatc cacctgatga acagaaccta gggaaagccc cagttctact tacaccagga 14821 aaggc //
pNL4-3载体图谱 pNL4.3载体HIV-1质粒图谱pdf版和pNL4-3载体序列 pNL4.3载体HIV-1质粒序列等相关资料下载
pNL4-3质粒 pNL4.3载体HIV-1载体应用举例
NL4-3-ΔEnv-EGFP
2020-11-25pNL4-3-Luc-R-E-
2020-11-25
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