| 质粒名称: | PCDNA3-myc-DNMT1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 36939 |
| 存储实验室: | Arthur Riggs |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5400 bp |
| 完整质粒大小: | 10251 bp |
| 载体修饰: | pcDNA3/Myc was constructed by Zhaoxia Chen (see Comments section for details). DNMT1 was cloned into the pcDNA3/Myc vector. |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | DNMT1 |
| 物种来源: | 人源 |
| 插入大小: | 4851 bp |
| GenBank编号 | X63692 |
| 启动子 | CMV |
| 标签/融合蛋白: | Myc (N terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3/Myc-DNMT1 was a gift from Arthur Riggs (爱迪基因 plasmid # 36939) |
| 参考文献部分: | The histone methyltransferase SETDB1 and the DNA methyltransferase DNMT3A interact directly and localize to promoters silenced in cancer cells. Li H, Rauch T, Chen ZX, Szabo PE, Riggs AD, Pfeifer GP. J Biol Chem. 2006 Jul 14;281(28):19489-500. Epub 2006 May 8. 10.1074/jbc.M513249200 PubMed 16682412 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pcDNA3/Myc-DNMT1 10297 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION Mammalian expression of human DNMT1 with myc tag.
ACCESSION .
VERSION .
KEYWORDS pcDNA3/Myc-DNMT1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10297)
AUTHORS Li H, Rauch T, Chen ZX, Szabo PE, Riggs AD, Pfeifer GP
TITLE The histone methyltransferase SETDB1 and the DNA methyltransferase
DNMT3A interact directly and localize to promoters silenced in
cancer cells.
JOURNAL J Biol Chem. 2006 Jul 14;281(28):19489-500. Epub 2006 May 8.
PUBMED 16682412
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|PCDNA3-myc-DNMT1|
FEATURES Location/Qualifiers
source 1..10297
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 895..904
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 904..933
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
promoter complement(5850..5868)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(5851..5868)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(5888..5905)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5894..6118
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 6164..6592
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6251..6270)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6461..6482
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6601..6621)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6606..6935
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6786..6921
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6848..6867
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 7002..7796
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(7056..7075)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7666..7685
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 7970..8091
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8007..8026)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8061..8080
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(8140..8156)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8140..8156)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8153..8175)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8164..8180
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8188..8218)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8233..8254
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8371..8388)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8542..9130)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8622..8641)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9301..10161)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9924..9943
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10162..10266)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgccacc
901 atggagcaga agctgatctc agaggaggac ctggaattca tgccggcgcg taccgcccca
961 gcccgggtgc ccacactggc cgtcccggcc atctcgctgc ccgacgatgt ccgcaggcgg
1021 ctcaaagatt tggaaagaga cagcttaaca gaaaaggaat gtgtgaagga gaaattgaat
1081 ctcttgcacg aatttctgca aacagaaata aagaatcagt tatgtgactt ggaaaccaaa
1141 ttacgtaaag aagaattatc cgaggagggc tacctggcta aagtcaaatc ccttttaaat
1201 aaagatttgt ccttggagaa cggtgctcat gcttacaacc gggaagtgaa tggacgtcta
1261 gaaaacggga accaagcaag aagtgaagcc cgtagagtgg gaatggcaga tgccaacagc
1321 ccccccaaac ccctttccaa acctcgcacg cccaggagga gcaagtccga tggagaggct
1381 aagcctgaac cttcacctag ccccaggatt acaaggaaaa gcaccaggca aaccaccatc
1441 acatctcatt ttgcaaaggg ccctgccaaa cggaaacctc aggaagagtc tgaaagagcc
1501 aaatcggatg agtccatcaa ggaagaagac aaagaccagg atgagaagag acgtagagtt
1561 acatccagag aacgagttgc tagaccgctt cctgcagaag aacctgaaag agcaaaatca
1621 ggaacgcgca ctgaaaagga agaagaaaga gatgaaaaag aagaaaagag actccgaagt
1681 caaaccaaag aaccaacacc caaacagaaa ctgaaggagg agccggacag agaagccagg
1741 gcaggcgtgc aggctgacga ggacgaagat ggagacgaga aagatgagaa gaagcacaga
1801 agtcaaccca aagatctagc tgccaaacgg aggcccgaag aaaaagaacc tgaaaaagta
1861 aatccacaga tttctgatga aaaagacgag gatgaaaagg aggagaagag acgcaaaacg
1921 acccccaaag aaccaacgga gaaaaaaatg gctcgcgcca aaacagtcat gaactccaag
1981 acccaccctc ccaagtgcat tcagtgcggg cagtacctgg acgaccctga cctcaaatat
2041 gggcagcacc caccagacgc ggtggatgag ccacagatgc tgacaaatga gaagctgtcc
2101 atctttgatg ccaacgagtc tggctttgag agttatgagg cgcttcccca gcacaaactg
2161 acctgcttca gtgtgtactg taagcacggt cacctgtgtc ccatcgacac cggcctcatc
2221 gagaagaata tcgaactctt cttttctggt tcagcaaaac caatctatga tgatgaccca
2281 tctcttgaag gtggtgttaa tggcaaaaat cttggcccca taaatgaatg gtggatcact
2341 ggctttgatg gaggtgaaaa ggccctcatc ggcttcagca cctcatttgc cgaatacatt
2401 ctgatggatc ccagtcccga gtatgcgccc atatttgggc tgatgcagga gaagatctac
2461 atcagcaaga ttgtggtgga gttcctgcag agcaattccg actcgaccta tgaggacctg
2521 atcaacaaga tcgagaccac ggttcctcct tctggcctca acttgaaccg cttcacagag
2581 gactccctcc tgcgacacgc gcagtttgtg gtggagcagg tggagagtta tgacgaggcc
2641 ggggacagtg atgagcagcc catcttcctg acgccctgca tgcgggacct gatcaagctg
2701 gctggggtca cgctgggaca gaggcgagcc caggcgaggc ggcagaccat caggcattct
2761 accagggaga aggacagggg acccacgaaa gccaccacca ccaagctggt ctaccagatc
2821 ttcgatactt tcttcgcaga gcaaattgaa aaggatgaca gagaagacaa ggagaacgcc
2881 tttaagcgcc ggcgatgtgg cgtctgtgag gtgtgtcagc agcctgagtg tgggaaatgt
2941 aaagcctgca aggacatggt taaatttggt ggcagtggac ggagcaagca ggcttgccaa
3001 gagcggaggt gtcccaatat ggccatgaag gaggcagatg acgatgagga agtcgatgat
3061 aacatcccag agatgccgtc acccaaaaaa atgcaccagg ggaagaagaa gaaacagaac
3121 aagaatcgca tctcttgggt cggagaagcc gtcaagactg atgggaagaa gagttactat
3181 aagaaggtgt gcattgatgc ggaaaccctg gaagtggggg actgtgtctc tgttattcca
3241 gatgattcct caaaaccgct gtatctagca agggtcacgg cgctgtggga ggacagcagc
3301 aacgggcaga tgtttcacgc ccactggttc tgcgctggga cagacacagt cctcggggcc
3361 acgtcggacc ctctggagct gttcttggtg gatgaatgtg aggacatgca gctttcatat
3421 atccacagca aagtgaaagt catctacaaa gccccctccg aaaactgggc catggaggga
3481 ggcatggatc ccgagtccct gctggagggg gacgacggga agacctactt ctaccagctg
3541 tggtatgatc aagactacgc gagattcgag tcccctccaa aaacccagcc aacagaggac
3601 aacaagttca aattctgtgt gagctgtgcc cgtctggctg agatgaggca aaaagaaatc
3661 cccagggtcc tggagcagct cgaggacctg gatagccggg tcctctacta ctcagccacc
3721 aagaacggca tcctgtaccg agttggtgat ggtgtgtacc tgccccctga ggccttcacg
3781 ttcaacatca agctgtccag tcccgtgaaa cgcccacgga aggagcccgt ggatgaggac
3841 ctgtacccag agcactaccg gaaatactcc gactacatca aaggcagcaa cctggatgcc
3901 cctgagccct accgaattgg ccggatcaaa gagatcttct gtcccaagaa gagcaacggc
3961 aggcccaatg agactgacat caaaatccgg gtcaacaagt tctacaggcc tgagaacacc
4021 cacaagtcca ctccagcgag ctaccacgca gacatcaacc tgctctactg gagcgacgag
4081 gaggccgtgg tggacttcaa ggctgtgcag ggccgctgca ccgtggagta tggggaggac
4141 ctgcccgagt gcgtccaggt gtactccatg ggcggcccca accgcttcta cttcctcgag
4201 gcctataatg caaagagcaa aagctttgaa gatcctccca accatgcccg tagccctgga
4261 aacaaaggga agggcaaggg aaaagggaag ggcaagccca agtcccaagc ctgtgagccg
4321 agcgagccag agatagagat caagctgccc aagctgcgga ccctggatgt gttttctggc
4381 tgcggggggt tgtcggaggg attccaccaa gcaggcatct ctgacacgct gtgggccatc
4441 gagatgtggg accctgcggc ccaggcgttc cggctgaaca accccggctc cacagtgttc
4501 acagaggact gcaacatcct gctgaagctg gtcatggctg gggagaccac caactcccgc
4561 ggccagcggc tgccccagaa gggagacgtg gagatgctgt gcggcgggcc gccctgccag
4621 ggcttcagcg gcatgaaccg cttcaattcg cgcacctact ccaagttcaa aaactctctg
4681 gtggtttcct tcctcagcta ctgcgactac taccggcccc ggttcttcct cctggagaat
4741 gtcaggaact ttgtctcctt caagcgctcc atggtcctga agctcaccct ccgctgcctg
4801 gtccgcatgg gctatcagtg caccttcggc gtgctgcagg ccggtcagta cggcgtggcc
4861 cagactagga ggcgggccat catcctggcc gcggcccctg gagagaagct ccctctgttc
4921 ccggagccac tgcacgtgtt tgctccccgg gcctgccagc tgagcgtggt ggtggatgac
4981 aagaagtttg tgagcaacat aaccaggttg agctcgggtc ctttccggac catcacggtg
5041 cgagacacga tgtccgacct gccggaggtg cggaatggag cctcggcact ggagatctcc
5101 tacaacgggg agcctcagtc ctggttccag aggcagctcc ggggcgcaca gtaccagccc
5161 atcctcaggg accacatctg taaggacatg agtgcattgg tggctgcccg catgcggcac
5221 atccccttgg ccccagggtc agactggcgc gatctgccca acatcgaggt gcggctctca
5281 gacggcacca tggccaggaa gctgcggtat acccaccatg acaggaagaa cggccgcagc
5341 agctctgggg ccctccgtgg ggtctgctcc tgcgtggaag ccggcaaagc ctgcgacccc
5401 gcagccaggc agttcaacac cctcatcccc tggtgcctgc cccacaccgg gaaccggcac
5461 aaccactggg ctggcctcta tggaaggctc gagtgggacg gcttcttcag cacaaccgtc
5521 accaaccccg agcccatggg caagcagggc cgcgtgctcc acccagagca gcaccgtgtg
5581 gtgagcgtgc gggagtgtgc ccgctcccag ggcttccctg acacctaccg gctcttcggc
5641 aacatcctgg acaagcaccg gcaggtgggc aatgccgtgc caccgcccct ggccaaagcc
5701 attggcttgg agatcaagct ttgtatgttg gccaaagccc gagagagtgc ctcagctaaa
5761 ataaaggagg aggaagctgc taaggactag gtcgaccgag atctctcgag gtaccgcggc
5821 cgctcgagca tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc
5881 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
5941 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
6001 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
6061 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
6121 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
6181 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
6241 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
6301 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
6361 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
6421 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
6481 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
6541 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
6601 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
6661 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
6721 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
6781 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
6841 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
6901 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
6961 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
7021 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
7081 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
7141 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
7201 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
7261 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
7321 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
7381 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
7441 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
7501 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
7561 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
7621 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
7681 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
7741 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
7801 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
7861 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
7921 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
7981 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
8041 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
8101 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
8161 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
8221 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
8281 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
8341 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
8401 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
8461 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
8521 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
8581 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
8641 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
8701 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
8761 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
8821 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
8881 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
8941 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
9001 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
9061 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
9121 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
9181 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
9241 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
9301 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
9361 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
9421 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
9481 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
9541 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
9601 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
9661 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
9721 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
9781 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
9841 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
9901 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
9961 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
10021 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
10081 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
10141 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
10201 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
10261 tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
