质粒名称: | pSLIK-GFP |
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出品公司: | 爱迪基因 |
目录编号: | 66844 |
存储实验室: | Christian Metallo |
载体骨架基本信息 |
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载体名称: | pSLIK-hygro |
载体出品公司: | Ian Frasier, Addgene Plasmid #25737 |
载体抗性: | 氨苄青霉素 |
空载体大小: | 13918 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 哺乳动物表达,慢病毒 |
筛选标记: | 潮霉素 |
高拷贝/低拷贝: | -- |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | GFP |
物种来源: | Aequorea victoria |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | CMV |
标签/融合蛋白: | -- |
克隆方法: | Gateway克隆 |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | CMV-F |
3' 测序引物 | huB-Cpro-R |
文献引用方法 |
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材料和方法部分: | pSLIK-GFP was a gift from Christian Metallo (Addgene plasmid # 66844 ; http://n2t.net/addgene:66844 ; RRID:Addgene_66844) |
参考文献部分: | Tracing compartmentalized NADPH metabolism in the cytosol and mitochondria of mammalian cells. Lewis CA, Parker SJ, Fiske BP, McCloskey D, Gui DY, Green CR, Vokes NI, Feist AM, Vander Heiden MG, Metallo CM. Mol Cell. 2014 Jul 17;55(2):253-63. doi: 10.1016/j.molcel.2014.05.008. Epub 2014 May 29. 10.1016/j.molcel.2014.05.008 PubMed 24882210 |
买家导航
pSLIKGFP载体基本信息
pSLIKGFP载体图谱质粒图谱和多克隆位点信息
pSLIKGFP载体简介
pSLIKGFP载体序列
LOCUS pSLIK-GFP 13529 bp ds-DNA circular SYN 21-DEC-2018 DEFINITION Tet-inducible expression of GFP in mammalian cells. ACCESSION . VERSION . KEYWORDS pSLIK-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13529) AUTHORS Lewis CA, Parker SJ, Fiske BP, McCloskey D, Gui DY, Green CR, Vokes NI, Feist AM, Vander Heiden MG, Metallo CM TITLE Tracing compartmentalized NADPH metabolism in the cytosol and mitochondria of mammalian cells. JOURNAL Mol Cell. 2014 Jul 17;55(2):253-63. doi: 10.1016/j.molcel.2014.05.008. Epub 2014 May 29. PUBMED 24882210 REFERENCE 2 (bases 1 to 13529) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 21, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13529 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 382..580 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 598..778 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 825..950 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1443..1676 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 2203..2320 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2377..2401 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind 2440..2710 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" promoter 2743..2781 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2778..2802 /label=LNCX /note="Human CMV promoter, forward primer" CDS 2888..3607 /codon_start=1 /product="enhanced GFP" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(2933..2954) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3194..3213) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3541..3562 /label=EGFP-C /note="EGFP, forward primer" protein_bind complement(3660..3684) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 3734..4945 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 4782..4801 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" CDS 5070..5816 /codon_start=1 /product="improved tetracycline-controlled transactivator" /label=rtTA-Advanced /note="In the Tet-On(R) system, rtTA-Advanced binds to the Tet-responsive element and stimulates transcription only in the presence of tetracycline or doxycycline." /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG" misc_feature 5974..6436 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(6030..6047) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 6257..6276 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6495..7514 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE" misc_feature 7980..8568 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8033..8053) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8451..8462) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(8571..8587) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8572..8588) /label=pBluescriptKS /note="For pBluescript vector" LTR 9097..9277 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(9303..9320) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 9309..9533 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 9579..10007 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9666..9685) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9876..9897 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(10016..10036) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 10021..10350 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 10201..10336 /label=SV40 ori /note="SV40 origin of replication" primer_bind 10263..10282 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 10398..10445 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 10464..10838 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 10968..11089 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(11005..11024) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 11059..11078 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(11138..11154) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(11138..11154) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(11151..11173) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 11162..11178 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(11186..11216) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11231..11252 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(11369..11386) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(11540..12128) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(11620..11639) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(12299..13159) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 12922..12941 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(13160..13264) /gene="bla" /label=AmpR promoter primer_bind complement(13339..13358) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg 601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga 841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa 901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc 961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat 1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg 1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc 1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc 1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa 1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat 1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta 1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga 1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca 1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat 1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag 1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg 1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg 1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa 1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa 1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac 1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt 1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca 2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa 2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg 2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg 2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac 2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag 2341 cagagatcca gtttggttaa ttctagacag ttatcaacaa gtttgtacaa aaaagcaggc 2401 tttaaaggaa ccaattcagt cgactggatc ctttaccact ccctatcagt gatagagaaa 2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac 2521 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata 2581 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga 2641 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca 2701 gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg 2761 gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca 2821 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccgaacta 2881 gtccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 2941 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 3001 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 3061 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca 3121 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 3181 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 3241 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 3301 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 3361 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 3421 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 3481 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca 3541 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 3601 caagtaaact agtcgactag ggataacagg gtaattgttt gaatgaggct tcagtctaga 3661 cccagctttc ttgtacaaag tggttgataa catctcgagc cataaagatg gttaattaac 3721 ccacccaaga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc 3781 acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg 3841 acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca gtatcagcag 3901 aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt tccagagagc 3961 ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc tccgtggggc 4021 ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag 4081 ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt ggtgagtagc 4141 gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt 4201 gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg 4261 gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa gacgcttgtg 4321 aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca agaacccaag 4381 gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg ctggggcacc 4441 atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg tcgtctgttg 4501 cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg 4561 ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt ggggccacct 4621 gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg gcctagggta 4681 ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag tgaggcgtca 4741 gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc ggttttgaac 4801 tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt ttgaaatgta 4861 atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg ctaaattctg 4921 gccgtttttg gcttttttgt tagacgaagc ttggtaccga gctcggatct ccaccccgta 4981 ccggtcctgc aggatttaaa tggcgcgccg ctagcgctac cggactcaga tctcgagctc 5041 aagcttcgaa ttctgcagtc gaattcacca tgtctagact ggacaagagc aaagtcataa 5101 acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg 5161 ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc 5221 tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg 5281 aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc 5341 tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg 5401 aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac 5461 tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc 5521 atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga 5581 gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg 5641 aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg 5701 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg 5761 atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaactaa 5821 gtaaggatcc gcggccgcac tagaggaatt ccgcccctct cccccccccc cctncccccc 5881 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt 5941 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt 6001 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa 6061 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac 6121 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg 6181 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt 6241 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca 6301 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt 6361 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg 6421 aaaaacacga tgataagctt accggtacgc gtgatagctt gccacaaccc gtaccaaaga 6481 tggatagatc cggaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa 6541 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca 6601 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct 6661 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc 6721 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg 6781 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg 6841 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac 6901 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc 6961 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc 7021 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg 7081 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga 7141 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt 7201 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag 7261 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct 7321 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc 7381 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga 7441 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga 7501 gggcaaagga atagagtaga tgccgaccga acaagagctg atttcgagaa cgcctcagcc 7561 agcaactcgc gcgagcctag caaggcaaat gcgagagaac ggccttacgc ttggtggcac 7621 agttctcgtc cacagttcgc taagctcgct cggctgggtc gcgggagggc cggtcgcagt 7681 gattcaggcc cttctggatt gtgttggtcc ccagggcacg attgtcatgc ccacgcactc 7741 gggtgatctg actgatcccg cagattggag atcgccgccc gtgcctgccg attgggtgca 7801 gatctctaga tctgtacaag taaagcggcc gcgactctag atcataatca gccataccac 7861 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca 7921 taaaatgaat gcaattgttg ttgtttagtc cctcccaatt cgatatcaag cttatcgata 7981 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 8041 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 8101 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 8161 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 8221 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 8281 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 8341 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg 8401 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 8461 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 8521 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc 8581 gacctcgatc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc 8641 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca 8701 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 8761 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat 8821 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca 8881 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag 8941 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat 9001 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt 9061 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc 9121 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 9181 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 9241 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc 9301 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 9361 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 9421 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 9481 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 9541 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 9601 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 9661 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 9721 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 9781 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 9841 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 9901 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 9961 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 10021 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 10081 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 10141 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 10201 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 10261 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 10321 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc 10381 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat 10441 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg 10501 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact 10561 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg 10621 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg 10681 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg 10741 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg 10801 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt 10861 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga 10921 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg 10981 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 11041 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta 11101 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 11161 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 11221 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 11281 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 11341 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 11401 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 11461 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 11521 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 11581 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 11641 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 11701 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 11761 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 11821 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 11881 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 11941 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 12001 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 12061 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 12121 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 12181 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 12241 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 12301 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 12361 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 12421 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 12481 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 12541 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 12601 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 12661 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 12721 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 12781 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 12841 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 12901 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 12961 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 13021 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 13081 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 13141 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 13201 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 13261 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc 13321 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct 13381 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag 13441 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct 13501 tcgcgatgta cgggccagat atacgcgtt //
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